期刊
TUMOR BIOLOGY
卷 39, 期 7, 页码 -出版社
SAGE PUBLICATIONS LTD
DOI: 10.1177/1010428317717123
关键词
Target-substituting subtractive SELEX technique; the serum of the lung cancer patients; tumor markers; nucleic acid aptamer
类别
资金
- Qinhuangdao Science and Technology Research and Development Plan, China [201501B034, 201401A105, 201501B051, 201502A193]
- Special Research Fund for the Doctoral Program of Higher Education [20121333120017]
- Hebei Province Key Research and Development Projects [17272402]
Lung cancer is by far the leading cause of cancer death in the world. Despite the improvements in diagnostic methods, the status of early detection was not achieved. So, a new diagnostic method is needed. The aim of this study is to obtain the highly specific nucleic acid aptamers with strong affinity to tumor markers in the serum of the lung cancer patients for targeting the serum. Aptamers specifically binding to tumor markers in the serum of the lung cancer patients were screened from the random single-stranded DNA library with agarose beads as supports and the serum as a target by target-substituting subtractive SELEX technique and real-time quantitative polymerase chain reaction technique. Subsequently, the secondary single-stranded DNA library obtained by 10 rounds of screening was amplified to double-stranded DNA, followed by high-throughput genome sequence analysis to screen aptamers with specific affinity to tumor markers in the serum of the lung cancer patients. Finally, six aptamers obtained by 10 rounds of screening were identified with high specific affinity to tumor markers in the serum of the lung cancer patients. Compared with other five aptamers, the aptamer 43 was identified both with the highest specificity to bind target molecule and without any obvious affinity to non-specific proteins. The screened aptamers have relatively high specificity to combine tumor markers in the serum of the lung cancer patients, which provides breakthrough points for early diagnosis and treatment of lung cancer.
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