Article
Cell Biology
Pan Wang, Lu Zhao, Sheng Gong, Shuanglong Xiong, Junwei Wang, Dewei Zou, Jinyu Pan, Yangmin Deng, Qian Yan, Nan Wu, Bin Liao
Summary: HIF1α and HIF2α regulate each other with a negative feedback loop, affecting GBM malignant progression through the EGFR-PI3K/AKT pathway and increasing sensitivity to chemotherapy. Their interaction with Sox2 and Klf4 further contributes to stemness expression and cell cycle arrest, enhancing malignancy in GBM.
CELL DEATH & DISEASE
(2021)
Article
Oncology
Mikella Robinson, Samuel F. Gilbert, Jennifer A. Waters, Omar Lujano-Olazaba, Jacqueline Lara, Logan J. Alexander, Samuel E. Green, Gregory A. Burkeen, Omid Patrus, Zinia Sarwar, Ryne Holmberg, Christine Wang, Carrie D. House
Summary: The findings suggest that SOX2 may be a more consistent indicator of ovarian tumor initiating cells (TICs) that contribute to tumor repopulation following chemotherapy. Further studies evaluating SOX2 in TIC biology will enhance understanding of mechanisms driving ovarian cancer relapse.
Article
Biochemistry & Molecular Biology
Han Thi Ngoc To, Qui Anh Le, Hang Thi Thuy Bui, Ji-Hong Park, Dongchul Kang
Summary: This study investigated the association of pluripotency factor expression with clinicopathological characteristics of gastric cancer patients, as well as changes in expressing these factors upon stem cell-enriching spheroid culture of gastric cancer cells, regulation of sphere-forming capacity, and response to cisplatin and TRAIL treatments by Nanog and KLF4. Nanog expression was associated with a new tumor and a worse prognosis in gastric cancer patients. The expression of pluripotency factors varied among six gastric cancer cells, with KLF4 and Nanog being highly expressed in SNU-601 and SOX2 being highly expressed in SNU-484.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Chiara Verdelli, Annamaria Morotti, Giulia Stefania Tavanti, Rosamaria Silipigni, Silvana Guerneri, Stefano Ferrero, Leonardo Vicentini, Valentina Vaira, Sabrina Corbetta
Summary: Tumors of the parathyroid glands exhibit expression of core stem genes, indicating an embryonic signature. Men1 and calcium-sensing receptor are involved in regulating the expression of these genes. In addition, short-term lithium chloride treatment can modulate the expression levels of stem cell genes in parathyroid adenomas.
Article
Multidisciplinary Sciences
Aileen Julia Riesle, Meijiang Gao, Marcus Rosenblatt, Jacques Hermes, Helge Hass, Anna Gebhard, Marina Veil, Bjorn Gruening, Jens Timmer, Daria Onichtchouk
Summary: This study investigates the combinatorial mechanisms of genome activation and shows that zebrafish genome activators Pou5f3 and Nanog exhibit different roles in the activation process. They act as pioneer-like transcription factors on synergistic enhancers, promoting histone acetylation and transcription activation. However, on antagonistic enhancers, one of the factors acts as a non-pioneer-like transcription factor, competing with the activator and blocking its effects. This activator-blocker mechanism restricts widespread transcriptional activation and prevents premature expression of late developmental genes in early embryos.
NATURE COMMUNICATIONS
(2023)
Article
Medicine, General & Internal
Yi-Jen Lee, Jehng-Kang Wang, Yu-Ming Pai, Alan Frost, Vip Viprakasit, Supachai Ekwattanakit, Hui-Chieh Chin, Jah-Yao Liu
Summary: This study successfully increased the population and plasticity of stem cells in human peripheral blood through suitable culture methods, showing increased expression of stemness markers and successful induction of multi-lineage differentiation. These findings highlight the importance of appropriate culture methods in increasing pluripotent gene expression and plasticity. Inducing leukocytes from human peripheral blood to differentiate into pluripotent potential cells is a significant breakthrough in regenerative medicine.
Article
Multidisciplinary Sciences
Ljudmila Klepinina, Aleksandr Klepinin, Laura Truu, Vladimir Chekulayev, Heiki Vija, Kaisa Kuus, Indrek Teino, Martin Pook, Toivo Maimets, Tuuli Kaambre
Summary: The study demonstrates that modulating cell metabolism through NaBT can be an effective strategy for treating colorectal cancer (CRC), with significant impacts on the energy metabolism and differentiation state of CRC cells.
Article
Chemistry, Multidisciplinary
Wei Yu, Min Liao, Yang Chen, Rui Xue, Xiao-Meng Shi, Dan Liu, Fang-Fang Zhuo, Hui Tang, Zhi-Yuan Lu, Peng-Fei Tu, Bo Han, Xin Jia, Ke-Wu Zeng
Summary: This study identified phosphatidyl-ethanolamine binding protein1 (PEBP1) as a cellular target of ethyl gallate (EG), a natural small molecule for ulcerative colitis (UC) treatment. EG inhibited the phosphorylation of PEBP1 and enhanced PEBP1-Raf-1 interaction to block the NF-kappa B inflammatory pathway in macrophages. It was also found that PEBP1 serves as an essential anti-inflammation mechanism in UC therapy.
CHEMICAL COMMUNICATIONS
(2023)
Article
Biotechnology & Applied Microbiology
Ying Ding, Xia Yuan, Yichen Zou, Jiachen Gao, Xianshuai Xu, Hongyan Sun, Qisheng Zuo, Yani Zhang, Bichun Li
Summary: This study found that OCT4, SOX2, and NANOG not only play a role in maintaining stem cell pluripotency but also coordinate glycolysis and participate in somatic-cell-induced reprogramming. This provides an important foundation for further research on the molecular mechanism of somatic-cell-induced reprogramming.
Article
Chemistry, Medicinal
Nattamon Hongwiangchan, Nicharat Sriratanasak, Duangdao Wichadakul, Nithikoon Aksorn, Supakarn Chamni, Pithi Chanvorachote
Summary: CIN-RM has been shown to suppress lung cancer stem cells by inhibiting their colony and tumor spheroid formation. It also affects central survival and stem cell regulatory proteins, leading to the depletion of CSC markers. The compound's mechanism of action involves inhibiting Akt/mTOR signaling and promoting c-Myc degradation.
Review
Genetics & Heredity
Marisol Aguirre, Manuela Escobar, Sebastian Forero Amezquita, David Cubillos, Camilo Rincon, Paula Vanegas, Maria Paula Tarazona, Sofia Atuesta Escobar, Juan Camilo Blanco, Luis Gustavo Celis
Summary: The transcription factors Oct4, Sox2, Klf4, and c-Myc enable the reprogramming of somatic cells into induced pluripotent cells, with potential applications in cancer and neurodegenerative disease therapies. However, there are limitations and risks that require further investigation before their use in humans.
Article
Chemistry, Medicinal
Seyed-Omar Zaraei, Nour N. Al-Ach, Hanan S. Anbar, Randa El-Gamal, Hamadeh Tarazi, Rimas T. Tokatly, Rawan R. Kalla, Mouna A. Munther, Marwa M. Wahba, Aya M. Alshihabi, Mahmoud K. Shehata, Rawan M. Sbenati, Afnan I. Shahin, Raafat El-Awady, Taleb H. Al-Tel, Mohammed I. El-Gamal
Summary: This article presents the design, synthesis, and biological screening results of a new series of diarylurea and diarylamide derivatives with a quinoline core armed with dimethylamino or morpholino side chains. The compounds showed broad-spectrum antiproliferative activity against a panel of 60 cancer cell lines, with three of them demonstrating higher potency than the reference drug, sorafenib. The compounds also exhibited high selectivity for C-RAF kinase, making them potential inhibitors for this molecular target.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Cell Biology
Md Mahfuz Al Mamun, Muhammad Riaz Khan, Yifu Zhu, Yuwei Zhang, Shuai Zhou, Ran Xu, Ihtisham Bukhari, Rick F. Thorne, Jinming Li, Xu Dong Zhang, Guangzhi Liu, Song Chen, Mian Wu, Xiaoyuan Song
Summary: This study identifies the E3 ubiquitin ligase Stub1 as a negative regulator of pluripotency in embryonic stem cells (ESCs). Stub1 catalyzes the degradation of Sox2, Oct4, and Nanog proteins, thereby reducing pluripotency. Deficiency of Stub1 enhances somatic cell reprogramming and delays differentiation, while its overexpression triggers ESC differentiation.
Article
Cell Biology
Yichi Xu, Xin Chen, Shuya Pan, Zhi-wei Wang, Xueqiong Zhu
Summary: TM7SF2 plays a crucial role in promoting cell proliferation and metastasis, inhibiting cell apoptosis, preventing G0/G1 phase arrest, and participating in tumorigenesis and progression through the C-Raf/ERK pathway in cervical cancer. The overexpression of TM7SF2 enhances xenograft tumor growth in vivo, indicating its potential as a therapeutic target for cervical cancer treatment in the future.
CELL DEATH DISCOVERY
(2021)
Article
Biochemistry & Molecular Biology
Lucas Simoes Machado, Camila Martins Borges, Marina Amaro de Lima, Juliano Rodrigues Sangalli, Jacinthe Therrien, Lais Vicari de Figueiredo Pessoa, Paulo Fantinato Neto, Felipe Perecin, Lawrence Charles Smith, Flavio Vieira Meirelles, Fabiana Fernandes Bressan
Summary: Mechanisms of cell reprogramming by pluripotency-related transcription factors or nuclear transfer seem to be mediated by similar pathways. The study suggests that OCT4 and SOX2 may contribute to both processes and help elucidate the mechanisms responsible for pluripotency.