Article
Multidisciplinary Sciences
Tianyan Liu, Till Stephan, Peng Chen, Jan Keller-Findeisen, Jingting Chen, Dietmar Riedel, Zhongtian Yang, Stefan Jakobs, Zhixing Chen
Summary: This study reports a mitochondrial inner membrane fluorescent marker, PK Mito Orange (PKMO), which features reduced phototoxicity and high stability, enabling super-resolution recordings of mitochondrial inner membrane dynamics. The PKMO dye is optically orthogonal with green and far-red markers, allowing multiplexed recordings. By using multi-color STED microscopy, interactions between mitochondria and other cellular components can be captured at sub-100 nm resolution.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biotechnology & Applied Microbiology
Andreas Boden, Francesca Pennacchietti, Giovanna Coceano, Martina Damenti, Michael Ratz, Ilaria Testa
Summary: A new 3D pRESOLFT microscope method with sub-80-nm resolution has been developed to visualize the volumetric architecture of organelles and molecules inside whole living cells, allowing for targeted 3D confinement of fluorescence and observation of dynamic structural alterations in cells.
NATURE BIOTECHNOLOGY
(2021)
Article
Nanoscience & Nanotechnology
Yujie Zheng, Yean Jin Lim, Hanqi Lin, Tienan Xu, Carmen Longbottom, Viviane Delghingaro-Augusto, Yee Lin Thong, Christopher R. Parish, Elizabeth E. Gardiner, Woei Ming Lee
Summary: This paper introduces a method to study cell migration using live cell imaging tools and proposes a combined system that integrates label-free nanoscale and fluorescence imaging. By empirically determining optimal scanning angles, quantitative 3D spatial temporal details of cellular components can be obtained. Using this combined imaging tool, researchers analyzed the migration of platelets and endothelial cells and made key findings, such as the importance of bovine serum albumin in platelet migration and the dynamics of mitochondria during cell migration.
Article
Biophysics
Tong Zhu, Guanqing Yang, Xiaolu Liu, Pan Xiang, Zhenghui Yang, Sijing Zhang, Juan Chen, Hong Wang, Senio Campos de Souza, Zhongping Zhang, Ruilong Zhang, Yupeng Tian, Jieying Wu, Xiaohe Tian
Summary: By rationally constructing a Py-BODIPY derivative, researchers successfully targeted the mitochondrial membrane in living cells without disturbing its physiological microenvironments, showing strong internalization capabilities in pre-fixed and stimulus disrupted samples. The probe's cristae specificity and excellent photostability allow for the observation of mitochondrial dynamic nano-structures with unprecedented resolution, demonstrating how mitochondrial 3D ultrastructure evolves under oxidative phosphorylation conditions.
BIOSENSORS & BIOELECTRONICS
(2021)
Review
Biochemistry & Molecular Biology
Herbert Schneckenburger
Summary: Laser plays an increasing role in live cell microscopy due to its unique properties. It is used in various techniques such as 3D microscopy, super-resolution microscopy, spectral imaging, and fluorescence lifetime imaging. Laser applications in live cell biology include fluorescence diagnosis, photodynamic therapy, and laser-assisted optoporation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Editorial Material
Optics
Sophie Brasselet
Summary: The combination of polarization modulation Fourier analysis and spatial information in a joint reconstruction algorithm for polarization-resolved fluorescence imaging improves spatial resolution and enables sensitive readout of anisotropy in cell samples.
LIGHT-SCIENCE & APPLICATIONS
(2022)
Article
Multidisciplinary Sciences
Jigmi Basumatary, Neptune Baro, Fancesca Cella Zanacchi, Partha Pratim Mondal
Summary: The blinking properties of a single molecule play a critical role in single-molecule localization microscopy (SMLM). A new method called short exposure-based SMLM (shortSMLM) using a high QE sCMOS detector is proposed for studying temporal dynamics in cellular systems. This technique improves the temporal resolution but sacrifices spatial resolution.
SCIENTIFIC REPORTS
(2023)
Review
Cell Biology
Li-An Chu, Shu-Wei Chang, Wei-Chun Tang, Yu-Ting Tseng, Peilin Chen, Bi-Chang Chen
Summary: Superresolution imaging allows visualization of detailed structures of organelles, with multi-dimensional imaging helping us understand cell function. Analyzing structural changes and molecular interactions across a large volume in 3D with different labelled targets is now necessary. Scientists have expanded 2D superresolution microscopic tools into 3D imaging techniques, focusing on reducing phototoxicity in live imaging of cell nucleus.
CURRENT OPINION IN GENETICS & DEVELOPMENT
(2021)
Article
Chemistry, Multidisciplinary
Lei G. Wang, Antonio R. Montano, Jason R. Combs, Nathan P. McMahon, Allison Solanki, Michelle M. Gomes, Kai Tao, William H. Bisson, Dani A. Szafran, Kimberley S. Samkoe, Kenneth M. Tichauer, Summer L. Gibbs
Summary: Non-destructive diffusion of fluorescent molecules across cell membranes is crucial for quantitative imaging in live cells and tissues. We have developed biologically compatible and cell-membrane-permeable fluorophores called OregonFluor (ORFluor), which allow for quantitative visualization of their intracellular distribution and protein target-specific binding using ratiometric imaging methodology. This provides a chemical toolkit for quantifying drug target availability in live cells and tissues.
Editorial Material
Biotechnology & Applied Microbiology
Giorgio Tortarolo, Suliana Manley
Summary: Super-resolution microscopy with angstrom precision could enable optical structural biology studies in cells.
NATURE BIOTECHNOLOGY
(2023)
Article
Optics
Shaocong Liu, Zhimin Zhang, Yubing Han, Lu Yang, Cuifang Kuang, Xu Liu
Summary: A novel method called ratiometric photon reassignment based on fluorescence lifetime is proposed in this study, which can improve the resolution and signal-to-noise ratio of super-resolution imaging without increasing the depletion power. Experimental results demonstrate that this method outperforms traditional STED with lower budget and data processing requirements.
Article
Chemistry, Multidisciplinary
Xinyi Kang, Wen Yang, Xu Gao, Ling Zhou, Ziyang Yu, Qun Dong, Yanyan Chen, Jianwu Dai, Hong Zhang
Summary: A novel engineered and biocompatible Janus collagen patch (JCOP) has been developed for treating uterine abnormalities infertility, achieving excellent uterine defect recovery and increasing effective live births.
ADVANCED MATERIALS INTERFACES
(2023)
Article
Optics
Wenhao Zhang, Kaidi Zhang, Wenting Wang, Yuhang Chen
Summary: Using s-SNOM and energy dispersive spectroscopy, we studied the effects of ion implantation in a silicon substrate, finding correlations between contrast and residual element concentration with ion dose, indicating the sensitivity of s-SNOM imaging to implanted ions.
Article
Chemistry, Analytical
Guoxuan Liu, Huaidong Yang, Hansen Zhao, Yinxin Zhang, Sichun Zhang, Xinrong Zhang, Guofan Jin
Summary: The study developed hyperspectral SIM technology, enabling high-speed spectral super-resolution imaging in SIM for the first time. By optically mapping the three-dimensional datacube of the sample to the detector plane, it allows simultaneous detection and retention of sample spectrum information.
ANALYTICAL CHEMISTRY
(2021)
Review
Biochemistry & Molecular Biology
Yuxin Li, Mingzhen Wang, Senlian Hong
Summary: A layer of glycans called the glycocalyx surrounds all cells in our bodies, and recent advances have allowed scientists to engineer it on live cells, leading to the creation of cellular drugs and novel medical treatments. Manipulating the structures and functions of cell-surface glycans has become possible, enabling the control of target cells and their properties for therapeutic applications.
Review
Microbiology
Peter N. Lipke, Stephen A. Klotz, Yves F. Dufrene, Desmond N. Jackson, Melissa C. Garcia-Sherman
MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS
(2018)
Article
Chemistry, Multidisciplinary
Valeria Prystopiuk, Cecile Feuillie, Philippe Herman-Bausier, Felipe Viela, David Alsteens, Giampiero Pietrocola, Pietro Speziale, Yves F. Dufrene
Article
Medicine, General & Internal
T. Bjarnsholt, K. Buhlin, Y. F. Dufrene, M. Gomelsky, A. Moroni, M. Ramstedt, K. P. Rumbaugh, T. Schulte, L. Sun, B. Akerlund, U. Romling
JOURNAL OF INTERNAL MEDICINE
(2018)
Article
Chemistry, Multidisciplinary
Cecile Feuillie, Claire Valotteau, Lionel Makart, Annika Gillis, Jacques Mahillon, Yves F. Dufrene
Article
Biochemistry & Molecular Biology
Victoria Vassen, Claire Valotteau, Cecile Feuillie, Cecile Formosa-Dague, Yves F. Dufrene, Xavier De Bolle
Article
Biochemistry & Molecular Biology
Giampiero Pietrocola, Giulia Nobile, Mariangela J. Alfeo, Timothy J. Foster, Joan A. Geoghegan, Vincenzo De Filippis, Pietro Speziale
JOURNAL OF BIOLOGICAL CHEMISTRY
(2019)
Article
Microbiology
Keenan A. Lacey, Michelle E. Mulcahy, Aisling M. Towell, Joan A. Geoghegan, Rachel M. McLoughlin
Article
Microbiology
Amy C. Pickering, Pauline Vitry, Valeriia Prystopiuk, Brandon Garcia, Magnus Hook, Jeffrey Schoenebeck, Joan A. Geoghegan, Yves F. Dufrene, J. Ross Fitzgerald
Article
Multidisciplinary Sciences
Samir Olatunji, Xiaoxiao Yu, Jonathan Bailey, Chia-Ying Huang, Marta Zapotoczna, Katherine Bowen, Maja Remskar, Rolf Mueller, Eoin M. Scanlan, Joan A. Geoghegan, Vincent Olieric, Martin Caffrey
NATURE COMMUNICATIONS
(2020)
Editorial Material
Microbiology
Charles J. Dorman, Joan Geoghegan
CURRENT OPINION IN MICROBIOLOGY
(2020)
Article
Multidisciplinary Sciences
Aisling M. Towell, Cecile Feuillie, Pauline Vitry, Thaina M. Costa, Marion Mathelie-Guinlet, Sanja Kezic, Orla M. Fleury, Maeve A. McAleer, Yves F. Dufrene, Alan D. Irvine, Joan A. Geoghegan
Summary: Staphylococcus aureus interacts with the host protein corneodesmosin on atopic dermatitis corneocytes, taking advantage of the aberrant display of corneodesmosin caused by low natural moisturizing factor levels. The bacterial proteins FnBPB and ClfB bind to the N-terminal region of corneodesmosin, facilitating strong adherence of S. aureus to atopic dermatitis corneocytes.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Multidisciplinary Sciences
Thaina M. da Costa, Albertus Viljoen, Aisling M. Towell, Yves F. Dufrene, Joan A. Geoghegan
Summary: This study identifies a novel loricrin-binding protein, FnBPB, in Staphylococcus aureus and demonstrates through single-molecule force spectroscopy that FnBPB strengthens the adhesion of S. aureus to human corneocytes through its strong binding to loricrin.
NATURE COMMUNICATIONS
(2022)
Article
Microbiology
Filippo Prencipe, Aishah Alsibaee, Zainab Khaddem, Padraig Norton, Aisling M. Towell, Afnan F. M. Ali, Gerard Reid, Orla M. Fleury, Timothy J. Foster, Joan A. Geoghegan, Isabel Rozas, Marian P. Brennan
Summary: Colonization of S. aureus can lead to systemic infections, which are difficult to treat due to antibiotic resistance. We have identified a small molecule inhibitor of the S. aureus clumping factors that has potential as a colonization inhibitor.
MICROBIOLOGY SPECTRUM
(2022)
Article
Nanoscience & Nanotechnology
Telmo O. Paiva, Albertus Viljoen, Thaina M. da Costa, Joan A. Geoghegan, Yves F. Dufrene
Summary: Attachment of Staphylococcus aureus to human skin corneocyte cells is mediated by bacterial cell-surface protein adhesins, including fibronectin-binding protein B (FnBPB). Using single-molecule experiments, it is demonstrated that FnBPB binds to corneodesmosin (CDSN) on atopic dermatitis patient corneocytes through a sophisticated two-site mechanism.
ACS NANOSCIENCE AU
(2023)
Article
Materials Science, Biomaterials
Marina Martinez-Carmona, Carmela Cela, Vera A. Kuznetsova, Joan A. Geoghegan, Yurii K. Gun'ko
Summary: The research demonstrates that chirality significantly affects the interaction of mesoporous silica nanoparticles with different cells and bacteria at the cellular level. Understanding the role of chirality in nanoparticles is crucial for the advancement of nano-biotechnology.
JOURNAL OF MATERIALS CHEMISTRY B
(2021)
