Article
Food Science & Technology
Jiale Zheng, Li Liu, Xiangmei Li, Zhenlin Xu, Zuoqi Gai, Xu Zhang, Hongtao Lei, Xing Shen
Summary: In this study, a rapid and sensitive method for detecting Burkholderia gladioli in food was developed using the CRISPR/Cas12a system combined with recombinant enzyme polymerase amplification (RPA). The optimized RPA-CRISPR/Cas12a assay was able to specifically detect Burkholderia gladioli at a constant temperature without the need for large equipment. The method showed a detection limit of 10(-3) ng/μL at the genomic DNA level and 10(1) CFU/mL at the bacterial quantity level. Real food samples, including rice noodles, fresh white noodles, and glutinous rice flour, were tested, and the method showed a detection limit of 10(1) CFU/mL, 10(2) CFU/mL, and 10(2) CFU/mL, respectively, without any enrichment steps. The entire detection process, including sample pretreatment and DNA extraction, could be completed within one hour. Compared to the qPCR method, the RPA-CRISPR/Cas12a method was simpler and more sensitive, allowing for rapid and easy visual detection of Burkholderia gladioli suitable for field detection.
Article
Biotechnology & Applied Microbiology
Natan De Jesus Pimentel-Filho, Stephan Fuchs, Maria Cristina Baracat-Pereira, Hilario Cuquetto Mantovani, Katharina Riedel, Maria Cristina Dantas Vanetti
Summary: This study investigates the stress response of Staphylococcus aureus to bovicin HC5 using a proteomic approach. The results show that sublethal concentrations of the bacteriocin repress protein synthesis, leading to growth reduction in the bacteria, while inducing certain proteins related to energy-efficient catabolism. This knowledge can help identify potential targets for controlling this pathogen.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Review
Food Science & Technology
Jin-Hee Kim, Se-Wook Oh
Summary: This review discusses techniques for detecting pathogens in food, including methods for separating and concentrating bacterial pathogens for molecular analysis. Pre-treatment techniques such as centrifugation, filtration, and immunomagnetic separation can reduce the time needed for cultural enrichment and overcome challenges in applicability across different food matrices.
Article
Food Science & Technology
Jeong-Eun Hyun, Sung-Kwon Moon, Sun-Young Lee
Summary: This study found that 460/470 nm LED illumination significantly reduced the levels of E. coli O157:H7, P. fluorescens, and L. monocytogenes, and induced oxidative stress leading to cell death by increasing intracellular ROS levels and causing damage to the membrane and DNA.
Article
Food Science & Technology
Zhengfei Yang, Weijia Zhang, Yongqi Yin, Weiming Fang, Huaiguo Xue
Summary: This article discusses the application of metal-organic frameworks (MOFs) in the preparation of sensors for toxins and pathogens analysis, including the synthesis methods of MOFs, introduction of toxins and pathogens, recent progress in MOF-based sensors, and challenges and future perspectives.
Article
Food Science & Technology
Xuan Weng, Cheng Zhang, Hai Jiang
Summary: Rapid and accurate detection of foodborne pathogens is crucial for preventing outbreaks, and microfluidic nanobiosensors show promise in achieving this by reducing assay time, enhancing sensitivity, and improving accuracy.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Food Science & Technology
Natharin Ngamwongsatit, Soraya Chaturongakul, Ratchaneewan Aunpad
Summary: Microbial contamination in foods can cause illnesses and significant losses in both the food industry and public health sectors. This study developed a multiplex PCR (m-PCR) assay for the rapid and reliable detection of six common foodborne pathogens and hygiene indicator microorganisms. The m-PCR showed comparable results to the gold-standard culture technique but had a significantly shorter processing time.
FOODBORNE PATHOGENS AND DISEASE
(2023)
Article
Chemistry, Analytical
Yao Zhang, Xingrui Liao, Gege Yu, Juan Wei, Peng Wang, Yueqi Wang, Yinnan Jing, Jiamin Wang, Pengyu Chen, Jianlong Wang, Hong Wang, Yanru Wang
Summary: The development of nanobodies devoid of the Fc fraction has been described for the first time, which enables the real-time monitoring of foodborne pathogens. The phage-mediated sandwich chemiluminescent enzyme immunoassay (CISA) showed high sensitivity for detecting Cronobacter sakazakii.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Yao Zhang, Xingrui Liao, Gege Yu, Juan Wei, Peng Wang, Yueqi Wang, Yinnan Jing, Jiamin Wang, Pengyu Chen, Jianlong Wang, Hong Wang, Yanru Wang
Summary: The development of stable, high-affinity, and low-cost nanoprobes is crucial for real-time monitoring of foodborne pathogens. This study describes the development of nanobodies devoid of the Fc fraction, which exhibit low cost, strong stability, high binding affinity, and adequate load capacity. By using a phage-mediated sandwich ELISA and a chemiluminescent enzyme immunoassay, the study achieved sensitive detection of Cronobacter sakazakii in dairy products.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Mohamed Fethi Diouani, Maher Sayhi, Zehaira Romeissa Djafar, Samir Ben Jomaa, Kamel Belgacem, Hayet Gharbi, Mihai Ghita, Laura-Madalina Popescu, Roxana Piticescu, Dhafer Laouini
Summary: Quality and food safety are major concerns and growing challenges in the world. Scientists are developing new means for the rapid and efficient detection and identification of bacteria in food and water resources. This study successfully used antibiotic-functionalized metallic nanoparticles to isolate and identify foodborne bacterial strains Bacillus cereus and Shigella flexneri.
Article
Food Science & Technology
Sazzad Hossen Toushik, Jun-Ha Park, Kyeongjun Kim, Md Ashrafudoulla, Mevo Senakpon Isaie Ulrich, Md Furkanur Rahman Mizan, Pantu Kumar Roy, Won-Bo Shim, Young-Mog Kim, Si Hong Park, Sang-Do Ha
Summary: The study shows that bioactive substances from lactic acid bacteria and plant-derived essential oils can act as biocidal agents, efficiently inhibiting biofilm formation on seafood processing surfaces and potentially serving as preservatives in the seafood industry.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Agriculture, Multidisciplinary
Fulong Deng, Zhongjun Zhao, Ruxin Wang, Chengfang Xiang, Yantong Lv, Wenwen Li, Yixiang Duan
Summary: A novel rapid detection method based on microbial volatile organic compounds (MVOCs) emitted from foodborne bacteria was established using ultraviolet photoionization time-of-flight mass spectrometry (UVP-TOF-MS). The results showed distinct differences in MVOCs among different species of bacteria, and characteristic MVOCs for each bacterium were selected using a feature selection algorithm. Online monitoring of MVOCs during bacterial growth revealed metabolomic patterns and the highest abundance of MVOCs during the logarithmic phase. The machine learning models achieved a good classification performance for different bacteria cultured in various food matrixes, with an accuracy of over 0.95. This study demonstrated the effective rapid detection of bacteria using MVOC analysis and its significant potential for bacterial monitoring in the food industry.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Food Science & Technology
Sazzad Hossen Toushik, Kyeongjun Kim, Md Ashrafudoulla, Md Furkanur Rahman Mizan, Pantu Kumar Roy, Shamsun Nahar, Younsoo Kim, Sang -Do Ha
Summary: This study isolated two LAB strains with probiotic potential from kimchi, and demonstrated their antibiofilm activity against foodborne pathogens on seafood models and food contact surfaces. The combined LAB isolates showed higher inhibition on both planktonic and biofilm cells after co-culture with the pathogens, highlighting their potential as green bioprotective agents in the seafood industry.
Article
Food Science & Technology
Xinyu Liao, Yue Zhang, Qiyi Zhang, Jianwei Zhou, Tian Ding, Jinsong Feng
Summary: This review systematically summarizes the advances in microfluidic systems for detecting foodborne pathogens, with a focus on different substrates and enabled functions. It discusses the integration of IoT for real-time food safety inspection and future directions in microfluidic technology for detecting and monitoring foodborne pathogens.
TRENDS IN FOOD SCIENCE & TECHNOLOGY
(2023)
Article
Microbiology
Nagham Anis, Laetitia Bonifait, Segolene Quesne, Louise Bauge, Marianne Chemaly, Muriel Guyard-Nicodeme
Summary: In Europe, a process hygiene criterion is set for Salmonella and Campylobacter on broiler carcasses after chilling. The reference methods for verifying compliance with the criterion are time-consuming and expensive. Therefore, a duplex PCR method was developed to simultaneously detect Salmonella spp. and quantify Campylobacter in the same sample after the pre-enrichment step for Salmonella recovery.
Article
Microbiology
Isidro Garcia-Menino, Pilar Lumbreras, Luz Leston, Monica Alvarez-Alvarez, Vanesa Garcia, Jens Andre Hammerl, Javier Fernandez, Azucena Mora
Summary: Analyzing the clonal structure and antimicrobial resistance of E. coli isolates in urinary tract infections is important for detecting changes in emerging uropathogenic clones and guiding optimal control and antibiotic prescribing.
MICROBIOLOGY SPECTRUM
(2022)
Article
Infectious Diseases
Mykhailo Savin, Gabriele Bierbaum, Nico T. Mutters, Ricarda Maria Schmithausen, Judith Kreyenschmidt, Isidro Garcia-Menino, Silvia Schmoger, Annemarie Kaesbohrer, Jens Andre Hammerl
Summary: This study characterizes carbapenem-resistant Klebsiella spp. in wastewater and surface water in Germany. The isolates showed resistance to multiple antibiotics and a high diversity of antibiotic-resistance genes. Virulence factors were also present in most isolates. The findings suggest the possible dissemination of resistant bacteria in the environment and the risk of colonization and infection in humans, livestock, and wildlife.
Article
Biochemistry & Molecular Biology
Jens Andre Hammerl, Sabrin El-Mustapha, Michelle Boelcke, Hannah Trampert, Andrea Barac, Claudia Jaeckel, Ashish K. Gadicherla, Stefan Hertwig
Summary: The study identified three groups of temperate phages in pathogenic Y. enterocolitica strains, each with different lytic activity against different strains.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Svenja Woudstra, Anneke Luecken, Nicole Wente, Yanchao Zhang, Stefanie Leimbach, Maya Katrin Gussmann, Carsten Kirkeby, Volker Kromker
Summary: Samples were collected from various locations in a dairy cow farm, and it was found that most Corynebacterium spp. isolated from milk samples were also present in the cows' environment. However, the most relevant species for intramammary infections, C. bovis, was not found in any environmental niche.
Article
Public, Environmental & Occupational Health
Hendrik Wilking, Sandra Beermann, Ides Boone, Johannes Dreesman, Volker Fingerle, Joern Gethmann, Raskit Lachmann, Marina Lamparter, Anne Mayer-Scholl, Anika Meinen, Meike Schoel, Beneditta Suwono
Summary: Bacterial zoonotic pathogens, which can be transferred between animals and humans, often cause diseases with severe outcomes. The transmission paths are diverse, including oral intake, respiratory infection, vector-borne transmission, etc. The emergence and spread of antibiotic-resistant bacterial pathogens are of great concern in public health.
BUNDESGESUNDHEITSBLATT-GESUNDHEITSFORSCHUNG-GESUNDHEITSSCHUTZ
(2023)
Article
Agriculture, Dairy & Animal Science
I. Hansson, A. Silvera, K. Ren, S. Woudstra, A. Skarin, W. F. Fikse, P. P. Nielsen, L. Ronnegard
Summary: Understanding the social interactions of dairy cows in group settings is crucial for improving herd management and optimizing animal health and welfare outcomes. This study aimed to identify the characteristics of cows that are associated with their number of social contacts in different functional areas, such as feeding and resting areas.
JOURNAL OF DAIRY SCIENCE
(2023)
Article
Agriculture, Dairy & Animal Science
S. Woudstra, N. Wente, Y. Zhang, S. Leimbach, M. K. Gussmann, C. Kirkeby, V. Kromker
Summary: To effectively prevent and control bovine mastitis, it is important to consider infection pathways and durations. A study was conducted on a commercial dairy herd, collecting quarter foremilk samples from all lactating cows over a period of 18 weeks. Different strains of bacteria were identified, with some causing more infections than others. The median infection durations varied for different pathogens, and contagious transmission was found to be a significant factor in longer-lasting infections.
JOURNAL OF DAIRY SCIENCE
(2023)
Article
Virology
Christian Willy, Joachim J. Bugert, Annika Y. Classen, Li Deng, Anja Duechting, Justus Gross, Jens A. Hammerl, Imke H. E. Korf, Christian Kuehn, Simone Lieberknecht-Jouy, Christine Rohde, Markus Rupp, Maria J. G. T. Vehreschild, Kilian Vogele, Sarah Wienecke, Martin Witzenrath, Silvia Wuerstle, Holger Ziehr, Karin Moelling, Felix Broecker
Summary: Bacteriophage therapy shows promise in addressing global and German antibiotic resistance crises. The current situation in Germany regarding phage therapy and research is outlined. Critical shortcomings include a lack of clinical trials, regulation, and phages for clinical use. Phage therapy is being used on a limited number of patients as individual treatment trials.
Article
Microbiology
Dorothee Serian, Yury Churin, Jens Andre Hammerl, Manfred Rohde, Arne Jung, Anja Mueller, Min Yue, Corinna Kehrenberg
Summary: In this study, seven Bordetella avium phages were isolated and characterized, showing broad host range and strong bacteriolytic activity. These phages used lipopolysaccharides as host receptors and exhibited a myovirus structure. Although phenotypically thought to be lytic, these phages were found to undergo a lysogenic phase, but infection did not confer stable host superinfection immunity. These findings are important for potential phage therapy against avian bordetellosis.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Svenja Woudstra, Nicole Wente, Yanchao Zhang, Stefanie Leimbach, Carsten Kirkeby, Maya Katrin Gussmann, Volker Kroemker
Summary: In order to design cost-effective prevention strategies for mastitis in dairy cow farms, it is important to understand the pathways through which the causative pathogens infect. This study investigated the sources of bacterial strains causing intramammary infections in one dairy cow herd. The results showed that Staphylococcus aureus can be spread between quarters during milking, highlighting the importance of measures to prevent its transmission.
Article
Veterinary Sciences
Andreas R. Boeker, Alexander Bartel, Phuong Do Duc, Antonia Hentzsch, Frederike Reichmann, Roswitha Merle, Heidi Arndt, Linda Dachrodt, Svenja Woudstra, Martina Hoedemaker
Summary: Regional benchmarking data is crucial for farmers to compare and improve the animal health situation of their herds. However, in German dairy cow farms, there is a lack of comprehensive data regarding udder health. This study aimed to investigate the incidence of clinical mastitis, evaluate udder health indicators, and assess the implementation of mastitis monitoring measures in different regions of Germany. The results can be used as benchmarks for farmers and advisors to assess the udder health situation in their herds.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Virology
Julia Anabell Braeuer, Jens Andre Hammerl, Sabrin El-Mustapha, Julius Fuhrmann, Andrea Barac, Stefan Hertwig
Summary: Telomere phages are a small group of temperate phages that replicate as linear plasmids and have a specific host specificity. Their unique characteristics make them important in research.
Article
Microbiology
Ulrike Binsker, Kathrin Oelgeschlaeger, Bernd Neumann, Guido Werner, Annemarie Kaesbohrer, Jens A. Hammerl
Summary: The mcr-1.26 gene, carried by E. coli isolated from poultry samples in Germany, was found to be mainly transmitted through plasmids. The genetic sequences of the plasmids in poultry and human isolates were highly similar, suggesting that poultry husbandry is the primary source of mcr-1.26.
MICROBIOLOGY SPECTRUM
(2023)
Article
Public, Environmental & Occupational Health
Idesbald Boone, Michele Luca D'Errico, Luigi Iannetti, Gaia Scavia, Rosangela Tozzoli, Steen Ethelberg, Tim Eckmanns, Klaus Stark, Hendrik Wilking, Sebastian Haller
Summary: Healthcare-associated foodborne outbreaks can cause serious illness and death, particularly in vulnerable hospital populations. A survey of 35 healthcare facilities in Germany and Italy investigated the availability and usability of electronic food menu data for investigations of these outbreaks. The study found significant variability in the storage time and formats of menu data in the surveyed facilities.
EPIDEMIOLOGY AND INFECTION
(2023)
Article
Microbiology
Quantao Zhang, Thomas Alter, Eckhard Strauch, Jens Andre Hammerl, Keike Schwartz, Maria Borowiak, Carlus Deneke, Susanne Fleischmann
Summary: This study characterized the virulence potential of NOVC strains isolated from retail seafood at both genotypic and phenotypic levels. The results showed differences in the virulence potential among the analyzed strains, which may have implications for future seafood monitoring and risk assessment.
Review
Food Science & Technology
Peiling Yan, Weiqing Lan, Jing Xie
Summary: Sodium alginate (SA) is an important marine polysaccharide that has extensive applications in food preservation and quality maintenance. Modification methods can be used to improve the properties of SA and make it compatible with other materials. Solvent-casting films and gels are common methods for using modified SA in food preservation.
TRENDS IN FOOD SCIENCE & TECHNOLOGY
(2024)
Article
Food Science & Technology
Zhihui Yu, Yating Gao, Xinyi Jia, Shuai Cui, Li Ma, Dan Zheng, Xin Li, Long Li, Lixin Zhang, Yisheng Chen
Summary: This review provides a new perspective on the state-of-the-art research and application of high-intensity ultrasound (HIU) for modifying blue food proteins (BFPs). The effects of HIU treatment on the functional properties and structural composition of BFPs are comprehensively evaluated.
TRENDS IN FOOD SCIENCE & TECHNOLOGY
(2024)
Review
Food Science & Technology
Honghong Zhang, Haoran Fan, Xueming Xu, Dan Xu
Summary: This review provides a comprehensive summary of changes in different components in frozen dough, including starch, gluten protein, lipid, and yeast, from a multiscale perspective. The methods of improving the production process of frozen dough, from main materials to end products, have been discussed. Key findings include the use of high gluten strength wheat flour and composite flour with high dietary fiber to improve the freezing resistance of gluten-starch matrix, as well as various methods to improve yeast freeze tolerance and the effects of different freezing rates.
TRENDS IN FOOD SCIENCE & TECHNOLOGY
(2024)
Article
Food Science & Technology
Junhua Xie, Qiqiong Li, Shaoping Nie
Summary: This review provides an overview of bEVs' biology, evidence for their health benefits, and possible molecular pathways involved in their health-promoting effects, as well as perspectives for applications in foods and pharmaceuticals.
TRENDS IN FOOD SCIENCE & TECHNOLOGY
(2024)
Article
Food Science & Technology
Jose Bueno-Mancebo, Adriana Artola, Raquel Barrena, Fernando Rivero-Pino
Summary: This review provides an overview of the characterization, production, and applications of sophorolipids (SLs) in the food industry. SLs exhibit bioactivity and environmental friendliness, making them suitable for various applications in food packaging, agriculture, and other areas. The importance of SLs in the context of the One Health approach is emphasized.
TRENDS IN FOOD SCIENCE & TECHNOLOGY
(2024)
Letter
Food Science & Technology
Saurabh Singh
Summary: This paper is interesting and relevant to determining the moisture content of food products. However, it lacks the classification of oven drying methods, which are cost-effective and important for drying solids and semisolids.
TRENDS IN FOOD SCIENCE & TECHNOLOGY
(2024)
Article
Food Science & Technology
Yong Cheng, Beijun Ang, Chaoyi Xue, Zhenyu Wang, Liduan Yin, Tong Wang, Qiuming Chen, Zhaojun Wang, Maomao Zeng, Wei Zhang, Jie Chen, Zhiyong He
Summary: Pollen fermentation can improve its nutrient profile, degrade allergenic compounds, enhance bioavailability, and promote gut health. Pollen can serve key roles in fermented food production and create opportunities for innovative product development. However, quality and safety considerations are important in pollen fermentation.
TRENDS IN FOOD SCIENCE & TECHNOLOGY
(2024)
Review
Food Science & Technology
Fanghua Guo, Fereidoon Shahidi
Summary: This contribution summarizes the potential of anthocyanins as alternatives to antibiotics, focusing on their anti-oxidation, anti-inflammation, absorption, metabolism, antibacterial effects, and the possible mechanisms behind these effects. The use of encapsulation to improve stability and bioavailability of anthocyanins, as well as targeted delivery to the lower digestive tract, shows promise in replacing antibiotics. However, more in vivo studies are needed to further support these findings.
TRENDS IN FOOD SCIENCE & TECHNOLOGY
(2024)
Article
Food Science & Technology
Muhammad Rehan Khan, Elena Torrieri, Florent Allais, Sami Fadlallah
Summary: This opinion paper explores the potential of integrating synthetic biology into microbial polymers to produce tailored biopolymers for food packaging applications. By leveraging synthetic biology tools and adopting safety assessment protocols established within the regulatory framework, this innovative approach has the potential to not only replace conventional methods but also address environmental concerns associated with traditional food packaging.
TRENDS IN FOOD SCIENCE & TECHNOLOGY
(2024)
