Article
Biophysics
Ye Tang, Hui Gao, Felix Kurth, Loic Burr, Konstantinos Petropoulos, Davide Migliorelli, Olivier T. Guenat, Silvia Generelli
Summary: LFIA is a well-established technique that provides immediate results, but suffers from high limits of detection. Introduction of a novel cellulose nanofiber aerogel material into LFIA strips has been shown to improve the detection sensitivity by extending the sample flow time.
BIOSENSORS & BIOELECTRONICS
(2021)
Review
Biochemistry & Molecular Biology
Jaehi Kim, Min-Sup Shin, Jonghyun Shin, Hyung-Mo Kim, Xuan-Hung Pham, Seung-min Park, Dong-Eun Kim, Young Jun Kim, Bong-Hyun Jun
Summary: Rapid, accurate, and convenient diagnosis is crucial for effective disease management, and lateral flow immunoassay (LFIA) with optical nanoparticles (NPs) has emerged as a major diagnostic tool. Researchers have developed various types of optical NPs with modified optical properties to serve as probes for LFIA. This review focuses on the literature about LFIA with optical NPs for specific target detection in diagnostics.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Sijie Liu, Xing Luo, Rui Shu, Yangjun Liao, Leina Dou, Tong Bu, Shaochi Wang, Yuechun Li, Jing Sun, Daohong Zhang, Mingqiang Zhu, Jianlong Wang
Summary: This study demonstrates the potential of stimulated surface-enhanced Raman scattering (SERS) combined with engineered nano-tracer in lateral flow immunoassays (LFIAs). The engineered core-shell multifunctional nano-tracer shows enhanced coupling efficiency, highly retained affinity, and reinforced colloid stability, leading to improved analysis performance.
Review
Biochemistry & Molecular Biology
Ge Li, Qingmei Li, Xun Wang, Xiao Liu, Yuhang Zhang, Rui Li, Junqing Guo, Gaiping Zhang
Summary: Lateral flow immunoassay (LFIA) is a popular technique in point-of-care testing (POCT) for its accessibility, speed, and affordability, and has gained significant attention during the COVID-19 outbreak for its ability to provide immediate diagnosis to users. This review introduces the principles, key components, and various detection formats of LFIA for antigens, antibodies, and haptens, as well as the integration of new trends such as novel labels, multiplexing, and digital assays. The development and future perspectives of LFIA are also discussed.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Review
Chemistry, Analytical
Donato Calabria, Maria Maddalena Calabretta, Martina Zangheri, Elisa Marchegiani, Ilaria Trozzi, Massimo Guardigli, Elisa Michelini, Fabio Di Nardo, Laura Anfossi, Claudio Baggiani, Mara Mirasoli
Summary: Paper-based lateral-flow immunoassays have achieved commercial success and are continuously growing in the field of diagnostics. These assays, which provide qualitative information about the target analyte, have the potential to be adapted for ultrasensitive quantitative analysis.
Article
Nanoscience & Nanotechnology
Jianhang Li, Guanbin Gao, Xintong Tang, Meng Yu, Meng He, Taolei Sun
Summary: The chemical and physical properties of nanobio interfaces can significantly impact the conformational transitions of adjacent biomolecules. This study investigates the isomeric effect of nanobio interfaces on the misfolding, aggregation, and fibrillation of A beta(40) using three different isomeric AuNCs. The results show that the inhibition efficiency of o-MBA-AuNCs is higher than that of m-MBA-AuNCs and p-MBA-AuNCs, providing insights for structure-based nanodrug design targeting Alzheimer's disease and other protein conformational diseases.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Biophysics
Natalia-Maria Christopoulou, Despina P. Kalogianni, Theodore K. Christopoulos
Summary: Lateral flow immunoassays (LFIA) are widely used for low-cost, simple, and rapid point-of-need testing. A study found that adding water-soluble macromolecular crowding agents can enhance the assay sensitivity without compromising its simplicity. The researchers demonstrated the improved sensitivity in detecting SARS-CoV-2 and Strep-A antigen using commercially available LFIA devices. The addition of macromolecular crowding agents is complementary to other sensitivity enhancement strategies and can potentially be applied to various analytes and LFIA configurations.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Food Science & Technology
Chunhao Wei, Aihong Wu, Liguang Xu, Chuanlai Xu, Liqiang Liu, Hua Kuang, Xinxin Xu
Summary: Foodborne pathogens can cause diseases in humans, and the traditional methods of detecting them are time-consuming. The lateral flow immunoassay (LFIA) is a popular detection platform for onsite testing of foodborne pathogens due to its time-efficiency, cost-effectiveness, portability, and ease of use. This review discusses the principles of LFIA and the use of nanomaterial labels to improve sensitivity in detecting pathogenic bacteria such as Escherichia coli, Vibrio parahaemolyticus, Staphylococcus aureus, Listeria monocytogenes, and Salmonella.
Review
Chemistry, Multidisciplinary
Jieying Liang, Kang Liang
Summary: Metal-organic frameworks (MOFs) have made significant progress in the field of bio-interface engineering, expanding the applications of biotechnology. Through design and integration with various biomacromolecules and living systems, MOFs exhibit unique interfacial interactions and application potential.
Article
Materials Science, Multidisciplinary
Jiuchuan Guo, Shuqin Chen, Jinhong Guo, Xing Ma
Summary: Lateral flow immunoassays (LFIAs) have rapidly developed and widely used in point-of-care-testing, offering advantages such as low cost, easy operation, and short time-consumption. To improve sensitivity, novel nanomaterials have been utilized as labels in LFIAs to generate various signals for quantitative or semi-quantitative detection.
JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY
(2021)
Article
Food Science & Technology
Xirui Chen, Xintao Miao, Tongtong Ma, Yuankui Leng, Liangwen Hao, Hong Duan, Jing Yuan, Yu Li, Xiaolin Huang, Yonghua Xiong
Summary: The study enhances the detection sensitivity of lateral flow immunoassay (LFIA) by synthesizing three different sizes of gold nanobeads (GNBs), with the 129 nm GNBs showing the best sensitivity for fumonisin B-1 (FB1) detection. The developed GNB-LFIA demonstrates improved performance compared to conventional LFIA based on 20-40 nm AuNPs.
Review
Chemistry, Multidisciplinary
Carlos Sanchez-Cano, Ramon A. Alvarez-Puebla, John M. Abendroth, Tobias Beck, Robert Blick, Yuan Cao, Frank Caruso, Indranath Chakraborty, Henry N. Chapman, Chunying Chen, Bruce E. Cohen, Andre L. C. Conceicao, David P. Cormode, Daxiang Cui, Kenneth A. Dawson, Gerald Falkenberg, Chunhai Fan, Neus Feliu, Mingyuan Gao, Elisabetta Gargioni, Claus-C Glueer, Florian Gruener, Moustapha Hassan, Yong Hu, Yalan Huang, Samuel Huber, Nils Huse, Yanan Kang, Ali Khademhosseini, Thomas F. Keller, Christian Koernig, Nicholas A. Kotov, Dorota Koziej, Xing-Jie Liang, Beibei Liu, Sijin Liu, Yang Liu, Ziyao Liu, Luis M. Liz-Marzan, Xiaowei Ma, Andres Machicote, Wolfgang Maison, Adrian P. Mancuso, Saad Megahed, Bert Nickel, Ferdinand Otto, Cristina Palencia, Sakura Pascarelli, Arwen Pearson, Oula Penate-Medina, Bing Qi, Joachim Raedler, Joseph J. Richardson, Axel Rosenhahn, Kai Rothkamm, Michael Rubhausen, Milan K. Sanyal, Raymond E. Schaak, Heinz-Peter Schlemmer, Marius Schmidt, Oliver Schmutzler, Theo Schotten, Florian Schulz, A. K. Sood, Kathryn M. Spiers, Theresa Staufer, Dominik M. Stemer, Andreas Stierle, Xing Sun, Gohar Tsakanova, Paul S. Weiss, Horst Weller, Fabian Westermeier, Ming Xu, Huijie Yan, Yuan Zeng, Ying Zhao, Yuliang Zhao, Dingcheng Zhu, Ying Zhu, Wolfgang J. Parak
Summary: X-ray-based analytics are widely used in physics, chemistry, materials science, and engineering, but their full potential in life sciences and medicine has not been fully utilized. Current and upcoming advances in applying these techniques to investigate the nano-bio interface are discussed, along with addressing current limitations and potential strategies for future in vivo use.
Article
Infectious Diseases
Sophie Owen, Christopher T. Williams, Gala Garrod, Alice J. Fraser, Stefanie Menzies, Lisa Baldwin, Lottie Brown, Rachel L. Byrne, Andrea M. Collins, Ana Cubas-Atienzar, Margaretha de Vos, Thomas Edwards, Camille Escadafal, Daniela M. Ferreira, Tom Fletcher, Angela Hyder-Wright, Grant A. Kay, Konstantina Kontogianni, Jenifer Mason, Elena Mitsi, Tim Planche, Jilian A. Sacks, Joseph Taylor, Stacy Todd, Caroline Tully, Luis E. Cuevas, Emily R. Adams
Summary: The evaluation of 12 LFAs showed varying sensitivity and specificity, with improved sensitivity for IgM/IgG over time since symptom onset. Compared to the EuroImmun IgG ELISA, LFAs exhibited different levels of sensitivity and specificity.
JOURNAL OF INFECTION
(2022)
Article
Materials Science, Multidisciplinary
Amadeo Sena-Torralba, Helena Torne-Morato, Claudio Parolo, Saba Ranjbar, Mohammad Amin Farahmand Nejad, Ruslan Alvarez-Diduk, Andrea Idili, Mohammad Reza Hormozi-Nezhad, Arben Merkoci
Summary: The majority of lateral flow assays use single-color labels, but this method has two limitations: it is prone to misinterpretation and does not allow precise modulation of sensitivity and dynamic range. To overcome these limitations, a ratiometric approach is developed that allows for adjustment of the assay's analytical properties.
ADVANCED MATERIALS TECHNOLOGIES
(2022)
Article
Biochemical Research Methods
Sian Sloan-Dennison, Emma O'Connor, James W. Dear, Duncan Graham, Karen Faulds
Summary: LFIA is vital for diagnosing biomolecules quickly at the POC, with SERS-based LFIA standing out as a method that enables identification and quantification of multiple targets simultaneously. Portable Raman instruments make it possible to conduct rapid and sensitive measurements at the POC, opening up new possibilities for healthcare in both urban and remote areas.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
