期刊
TRENDS IN BIOTECHNOLOGY
卷 35, 期 4, 页码 347-359出版社
ELSEVIER SCIENCE LONDON
DOI: 10.1016/j.tibtech.2016.09.003
关键词
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资金
- National Natural Science Foundation of China [51573063, 21174044, 21674040, 51403042]
- Guangdong Science and Technology Planning Project [2014B010104004, 2013B090600126]
- Natural Science Foundation for Distinguished Young Scholars of Guangdong Province [2016A030306013]
- Guangdong Program for Support of Top-Notch Young Professionals [2015TQ01R604]
- Guangzhou Science and Technology Planning Project [201604010013]
- Scientific Research Projects of Guangzhou [201607010328]
Heterogeneities and oncogenesis essentially result from proteomic disorders orchestrated by changes in DNA and/or cytoplasmic mRNA. These genetic fluctuations, however, cannot be decoded through conventional label-free methods (e.g., patch clamps, electrochemical cellular biosensors, etc.) or morphological characterization. Molecular beacons (MBs) have recently emerged as efficient probes for interrogating biomarkers in live cancer cells. MBs hybridize with their intracellular targets (e.g., mRNAs, DNAs, or proteins), emitting a fluorescent signal that can be quantified and correlated with the expression levels of their targets. In this review we discuss MB probes with different delivery platforms for intracellular probing as well as novel MB designs for detecting a variety of targets in living cancer cells. Finally, we describe current trends in MB based intracellular biosensors.
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