Review
Cell Biology
Akihiko Nakano
Summary: The structure of the Golgi apparatus varies among different species, but its functions remain conserved. In addition to the core layers of cisternae, the Golgi is accompanied by neighboring compartments on its cis and trans sides. High-resolution live imaging has revealed striking similarities in the behaviors of these compartments, indicating the presence of conserved mechanisms.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Sen Qiao, Xiaofang Luo, Hui Wang, Yue Fang, Lili Zhang
Summary: Valproic acid (VPA) affects the cell wall integrity (CWI) in the yeast S. pombe primarily through the target gene vas4(+) / vrg4(+), which encodes the Golgi GDP-mannose transporter Vrg4.
SCIENTIFIC REPORTS
(2021)
Article
Cell Biology
Daniel D. Bisinski, Ines Gomes Castro, Muriel Mari, Stefan Walter, Florian Froehlich, Maya Schuldiner, Ayelen Gonzalez Montoro
Summary: Membrane contact sites are specialized platforms between organelles that facilitate metabolite exchange and influence dynamics. This study identifies a novel protein, Cvm1, that forms multiple contact sites in the yeast vacuole and plays a crucial role in sphingolipid homeostasis.
JOURNAL OF CELL BIOLOGY
(2022)
Article
Biology
Sean Rogers, Hanaa Hariri, N. Ezgi Wood, Natalie Ortiz Speer, W. Mike Henne
Summary: This study reveals that yeast undergo metabolic remodeling at nucleus-vacuole junctions in response to acute glucose restriction, retaining HMGCRs and promoting mevalonate pathway flux in a spatially coordinated manner. The protein Nvj1 plays a crucial role in this process, demonstrating the importance of inter-organelle contacts in organizing metabolic reactions. Loss of HMGCR compartmentalization perturbs yeast growth following glucose starvation, indicating its role in adaptive metabolic remodeling.
Article
Biochemistry & Molecular Biology
Henning Arlt, Babu Raman, Yasmina Filali-Mouncef, Yan Hu, Alexandre Leytens, Ralph Hardenberg, Rodrigo Guimaraes, Franziska Kriegenburg, Muriel Mari, Iwona I. Smaczynska-de Rooij, Kathryn R. Ayscough, Christian Ungermann, Joern Dengjel, Fulvio Reggiori
Summary: Autophagy is a crucial cellular process that maintains homeostasis by degrading cellular components. This study reveals that Vps1 mutants affect the subcellular distribution of Atg9 and impair autophagy. The findings provide new insights into the role of dynamins in Atg9 trafficking and their potential contribution to severe human pathologies associated with autophagy defects.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Natsuki Omae, Yuka Sameshima-Yamashita, Kazunori Ushimaru, Hideaki Koike, Hiroko Kitamoto, Tomotake Morita
Summary: This study confirmed the relationship between proteases A and B orthologous genes in Pseudozyma antarctica and the degradation of biodegradable plastic-degrading enzyme (PaE), providing a new approach for large-scale production of PaE. The gene-deletion mutants showed stable production of PaE without degradation fragments, indicating the importance of proteases A and B in controlling PaE degradation.
Article
Biochemistry & Molecular Biology
Akane Sato, Takumi Kimura, Kana Hondo, Miyuki Kawano-Kawada, Takayuki Sekito
Summary: The GATA transcription factors, including Gln3 and Gat1, play a crucial role in regulating the expression of AVT transporters in cellular amino acid homeostasis. Mutations in the putative GATA-binding sites of the AVT4 promoter reduced AVT4 expression, indicating the importance of GATA factors in coordinating vacuolar amino acid transport. Prior microarray results also confirmed that other AVT transporters, such as AVT1, AVT6, and AVT7, are direct targets of GATA factors.
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2021)
Article
Agriculture, Multidisciplinary
Pingping Zhou, Yi Du, Xin Fang, Nannan Xu, Chunlei Yue, Lidan Ye
Summary: By combinational regulation of linalool synthase and farnesyl diphosphate synthase, a stress-free linalool-producing yeast cell factory was constructed, achieving the highest reported linalool production in yeast and showing potential for producing other high-value monoterpenes.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Agriculture, Multidisciplinary
Pingping Zhou, Xiuqi Zhou, Doudou Yuan, Xin Fang, Xueqing Pang, Ke Yuan, Aipeng Li, Xiqing Wang
Summary: In this study, the entrance of the substrate-binding pocket of linalool synthase was engineered to increase its catalytic efficiency, resulting in increased linalool production. Additionally, the use of cytoplasm and peroxisomes boosted linalool synthesis in Saccharomyces cerevisiae, achieving the highest linalool production in yeast to date.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Review
Plant Sciences
Yoko Ito, Tomohiro Uemura
Summary: Compared to the static image of plants, the world inside each cell is dynamic. Visualizing the working components inside cells using super-resolution microscopy is crucial for understanding cellular events, particularly in the study of plant membranes.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Cell Biology
Yoko Kagohashi, Michiko Sasaki, Alexander I. May, Tomoko Kawamata, Yoshinori Ohsumi
Summary: This study provides insights into the function and mechanism of Atg15 phospholipase, further understanding the disruption of autophagosome inner membrane structure.
JOURNAL OF CELL BIOLOGY
(2023)
Article
Multidisciplinary Sciences
David M. Hollenstein, Mariya Licheva, Nicole Konradi, David Schweida, Hector Mancilla, Muriel Mari, Fulvio Reggiori, Claudine Kraft
Summary: Vac8 plays a crucial role in coordinating the initiation of autophagosome formation by promoting the assembly of autophagy factors through avidity-driven interactions. In selective autophagy, Vac8 acts as a central hub to nucleate the phagophore assembly site at the vacuolar membrane, leading to clustering and sequestering of early autophagy factors and promoting phagophore assembly.
NATURE COMMUNICATIONS
(2021)
Article
Agriculture, Multidisciplinary
Junhua Wang, Linghuan Zhu, Youran Li, Sha Xu, Wei Jiang, Chaojuan Liang, Yakun Fang, Alex Chu, Liang Zhang, Zhongyang Ding, Guiyang Shi
Summary: By using a GGPP synthase with stronger catalytic ability and increasing the metabolic flux of the MVA pathway and the supply of IPP, the production of GGOH in Saccharomyces cerevisiae was significantly enhanced. These strategies can also be applied to enhance the production of other terpenoids in the future.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Veterinary Sciences
Annalisa Scollo, Irene Borello, Marco Ghilardi, Alberto Cavagnini
Summary: This study investigated the effects of a postbiotic from inactivated and stabilized whole-cells of Saccharomyces cerevisiae on the mother and litter of pigs. The results showed that dietary supplementation of this postbiotic during the gestation of sows can potentially improve lactation efficiency, reduce piglet mortality, and decrease antibiotic use during the weaning phase. The findings highlight the importance of using postbiotics in modern pig farming to improve animal health and production efficiency.
VETERINARY SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Rui Ma, Ping Su, Juan Guo, Baolong Jin, Qing Ma, Haiyan Zhang, Lingli Chen, Liuying Mao, Mei Tian, Changjiangsheng Lai, Jinfu Tang, Guanghong Cui, Luqi Huang
Summary: This study identified the highly specific (+)-bornyl diphosphate synthase from Cinnamomum burmanni and successfully reconstructed the (+)-borneol biosynthetic pathway in Saccharomyces cerevisiae, significantly increasing the yield. This lays the foundation for further synthesis and production of this valuable monoterpene compound.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Marcus Hoering, Christer S. Ejsing, Sabrina Krautbauer, Verena M. Ertl, Ralph Burkhardt, Gerhard Liebisch
Summary: This study evaluated the accuracy of FTMS quantification of lipid species affected by Type-II overlap, demonstrating that accurate quantification of lipid species by FTMS requires resolution-depended data analysis.
JOURNAL OF LIPID RESEARCH
(2021)
Article
Nutrition & Dietetics
Nicole L. Henriksen, Karoline Aasmul-Olsen, Ramakrishnan Venkatasubramanian, Mikkel K. E. Nygaard, Richard R. Sprenger, Anne B. Heckmann, Marie S. Ostenfeld, Christer S. Ejsing, Simon F. Eskildsen, Anette Mullertz, Per T. Sangild, Stine B. Bering, Thomas Thymann
Summary: This study found that emulsification of fat globules in infant formula with dairy-derived emulsifiers altered the plasma lipid profile and hippocampal tissue diffusivity, but had limited effects on other absorptive and learning abilities.
Article
Cell Biology
Richard R. Sprenger, Martin Hermansson, Ditte Neess, Lena Sokol Becciolini, Signe Bek Sorensen, Rolf Fagerberg, Josef Ecker, Gerhard Liebisch, Ole N. Jensen, Dennis E. Vance, Nils J. Faergeman, Robin W. Klemm, Christer S. Ejsing
Summary: This study investigates the diurnal regulation of whole-body lipid metabolism through time-series multi-omics analyses of liver and plasma, revealing that molecular oscillations are mainly entrained by adaptations to fasting, food intake, and the postprandial state. The research also highlights the key role of the hepatic phosphatidylethanolamine (PE) methylation pathway in diurnal regulation, leading to the production of two pools of oscillating phosphatidylcholine (PC) molecules in the circulation.
Article
Biotechnology & Applied Microbiology
Steffen Schmidt, Sandra F. Gallego, Iris Daphne Zelnik, Sergey Kovalchuk, Nanna Albaek, Richard R. Sprenger, Charlotte Overup, Yael Pewzner-Jung, Anthony H. Futerman, Marie W. Lindholm, Ole N. Jensen, Christer S. Ejsing
Summary: Emerging clinical data show that three ceramide molecules, Cer d18:1/16:0, Cer d18:1/24:1, and Cer d18:1/24:0, are biomarkers of a fatal outcome in patients with cardiovascular disease. This study developed a potent N-acetylgalactosamine-conjugated antisense oligonucleotide that specifically targets ceramide synthase 2 in hepatocytes. Results demonstrate that this compound effectively reduces ceramide synthase 2 mRNA level, leading to lower protein expression and activity as well as ceramide levels in the liver. Interestingly, the hepatocyte-specific antisense oligonucleotide also modulates blood plasma ceramides, suggesting that these biomarkers are regulated by ceramide biosynthesis in hepatocytes.
Article
Biochemistry & Molecular Biology
Mike F. Renne, Xue Bao, Margriet W. J. Hokken, Adolf S. Bierhuizen, Martin Hermansson, Richard R. Sprenger, Tom A. Ewing, Xiao Ma, Ruud C. Cox, Jos F. Brouwers, Cedric H. De Smet, Christer S. Ejsing, Anton I. P. M. de Kroon
Summary: Our study reveals the importance of ERMES and Vps39p in mediating the transfer of di-unsaturated phospholipids to mitochondria. This supports the concept that lipid flow between membranes is limited by molecular species-dependent lipid efflux and concentration gradients between membranes.
Review
Biochemistry & Molecular Biology
Shamroop Kumar Mallela, Sandra Merscher, Alessia Fornoni
Summary: Sphingolipids play important roles in cellular processes and abnormalities are associated with kidney diseases. Understanding the regulation of sphingolipid signaling in kidney diseases can lead to the development of therapeutics.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Nicole L. Henriksen, Karina S. Asmussen, Xiaoyu Pan, Ping-Ping Jiang, Yuki Mori, Line Christiansen, Richard R. Sprenger, Christer S. Ejsing, Stanislava Pankratova, Thomas Thymann
Summary: This study found that the fat source in diet can influence the plasma and hippocampal lipidome in three-day-old piglets, but is unable to improve brain structural and functional impairments induced by IUGR.
SCIENTIFIC REPORTS
(2022)
Article
Biochemical Research Methods
Saleh M. Khalil, Richard R. Sprenger, Martin Hermansson, Christer S. Ejsing
Summary: Matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI) is a useful technique for visualizing the spatial distribution of lipid molecules in tissues. The limitation of confident lipid identification in MSI has been overcome by the development of DDA-imaging technology. This study demonstrates the successful transfer of DDA-imaging technology onto another mass spectrometer and shows its reliability in lipid identification.
JOURNAL OF MASS SPECTROMETRY
(2022)
Article
Physiology
Nicole Lind Henriksen, Svend Hoime Hansen, Matthew Domenic Lycas, Xiaoyu Pan, Thomas Eriksen, Lars Sondergaard Johansen, Richard R. Sprenger, Christer Stenby Ejsing, Douglas G. Burrin, Kerstin Skovgaard, Vibeke Brix Christensen, Thomas Thymann, Stanislava Pankratova
Summary: Neonatal cholestasis affects the molecular bile acid and lipid composition of the brain, which may contribute to motor deficits.
PHYSIOLOGICAL REPORTS
(2022)
Article
Urology & Nephrology
Alla Mitrofanova, Antonio Fontanella, Matthew Tolerico, Shamroop Mallela, Judith Molina David, Yiqin Zuo, Marcia Boulina, Jin-Ju Kim, Javier Santos, Mengyuan Ge, Alexis Sloan, Wadih Issa, Margaret Gurumani, Jeffrey Pressly, Marie Ito, Matthias Kretzler, Sean Eddy, Robert Nelson, Sandra Merscher, George Burke, Alessia Fornoni
Summary: This study reveals the crucial role of STING activation in the progression of DKD and Alport syndrome, suggesting that targeting STING may provide a therapeutic option for glomerular diseases.
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
(2022)
Editorial Material
Biochemistry & Molecular Biology
Shamroop Kumar Mallela, Mengyuan Ge, Judith Molina, Javier Varona Santos, Jin-Ju Kim, Alla Mitrofanova, Hassan Al-Ali, Brian Marples, Sandra Merscher, Alessia Fornoni
Article
Multidisciplinary Sciences
Ewa Sitarska, Silvia Dias Almeida, Marianne Sandvold Beckwith, Julian Stopp, Jakub Czuchnowski, Marc Siggel, Rita Roessner, Aline Tschanz, Christer Ejsing, Yannick Schwab, Jan Kosinski, Michael Sixt, Anna Kreshuk, Anna Erzberger, Alba Diz-Munoz
Summary: A curvature-sensing mechanism mediated by the BAR domain protein Snx33 is identified in immune-like cells for obstacle evasion. Actin polymerization at the leading edge of migrating cells is inhibited by Snx33 in regions with inward plasma membrane curvature. Genetic perturbation of this machinery reduces cells' ability to evade obstacles and increases their speed and persistence in obstacle-free environments. This study reveals how cells interpret their environment using actin and plasma membrane biophysics.
NATURE COMMUNICATIONS
(2023)
Article
Cell Biology
Sergej Limar, Carolin Koerner, Fernando Martinez-Montanes, Viktoriya G. Stancheva, Verena N. Wolf, Stefan Walter, Elizabeth A. Miller, Christer S. Ejsing, Vanesa Viviana Galassi, Florian Froehlich
Summary: Limar et al. discovered that the yeast protein Svf1 localizes to the cis Golgi apparatus and is involved in the transport of ceramides between the ER and the Golgi. Svf1 binds ceramides and plays a role in maintaining appropriate levels of complex sphingolipids. This study provides insights into the mechanism of ceramide transport in yeast cells and its significance in sphingolipid metabolism.
JOURNAL OF CELL BIOLOGY
(2023)
Article
Endocrinology & Metabolism
Jeffrey G. McDonald, Christer S. Ejsing, Dominik Kopczynski, Michal Holcapek, Junken Aoki, Makoto Arita, Masanori Arita, Erin S. Baker, Justine Bertrand-Michel, John A. Bowden, Britta Brugger, Shane R. Ellis, Maria Fedorova, William J. Griffiths, Xianlin Han, Jurgen Hartler, Nils Hoffmann, Jeremy P. Koelmel, Harald C. Kofeler, Todd W. Mitchell, Valerie B. O'Donnell, Daisuke Saigusa, Dominik Schwudke, Andrej Shevchenko, Candice Z. Ulmer, Markus R. Wenk, Michael Witting, Denise Wolrab, Yu Xia, Robert Ahrends, Gerhard Liebisch, Kim Ekroos
Summary: The rapid growth of lipidomic data has led to a community-based movement to develop guidelines and minimum requirements for generating, reporting, and publishing such data. The creation of a dynamic checklist summarizing key details of lipidomic analyses using a common language has the potential to harmonize the field by improving traceability and reproducibility.
Meeting Abstract
Medicine, General & Internal
Manuel Hertel, Sahra Quahoud, Daniel Markgraf, Fernando Martinez-Montanes, Christer Ejsing, Michael Roden
WIENER KLINISCHE WOCHENSCHRIFT
(2021)