Article
Biochemistry & Molecular Biology
Chen Feng, Etienne Empweb Anger, Xiong Zhang, Shengdi Su, Chenlin Su, Shuxin Zhao, Feng Yu, Ji Li
Summary: This study suggests that UCP2 may protect HK-2 cells from the toxicity induced by AA I, and reduce the toxicity by decreasing oxidative stress and apoptosis. UCP2 could be a potential target for alleviating AA I-induced nephrotoxicity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Food Science & Technology
Yen-Chuan Ou, Jian-Ri Li, Chih-Cheng Wu, Tung-Min Yu, Wen-Ying Chen, Su-Lan Liao, Yu-Hsiang Kuan, Yu-Fan Chen, Chun-Jung Chen
Summary: Cadmium induces kidney toxicity through oxidative stress and inflammation. This study investigates the reciprocal crosstalk among oxidative stress, inflammation, and the nuclear Nrf2 pathway in cadmium-induced nephrotoxicity. The results suggest a role for HO-1 as a linking molecule in mediating nephrotoxicity-associated IL-6 expression upon cadmium exposure.
FOOD AND CHEMICAL TOXICOLOGY
(2022)
Article
Endocrinology & Metabolism
Yanqi Ma, Qikai Zhou, Pingping Zhao, Xiaoyu Lv, Caixia Gong, Jie Gao, Jingfang Liu
Summary: This study observed the glycation degree and sites of transferrin induced by different concentrations of high glucose, and investigated the effects of high glucose on human renal tubular epithelial cells. The results showed that the glycation degree of transferrin increased with high glucose stimulation, but the binding capacity with iron decreased. Additionally, in vitro stimulation of HK-2 cells with AGE-Tf led to increased cell apoptosis, decreased antioxidant capacity, and downregulated TfR expression levels.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Wenjing Li, Libing Yu, Bo Fu, Jian Chu, Chun Chen, Xijian Li, Jiahua Ma, Wei Tang
Summary: The study found that Polygonatum kingianum (PK) can effectively detoxify and prevent uranium-induced nephrotoxicity. Polysaccharides and aqueous extract of PK can enhance metabolic activity, alleviate cell damage and apoptosis. The study also found that PK alleviates uranium-induced cytotoxicity by regulating mitochondria-mediated apoptosis and the GSK-3 beta/Fyn/Nrf2 pathway.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Multidisciplinary Sciences
Chenglin Zou, Xun Tang, Tingting Guo, Tingting Jiang, Wenying Zhang, Jun Zhang
Summary: Cisplatin is an effective anti-tumor drug but can cause acute kidney injury (AKI). CTRP3, a new adipokine, has been found to have antioxidant, anti-inflammatory, and antiapoptotic properties. This study demonstrates that CTRP3 protects against cisplatin-induced AKI through the Nrf2/MAPK pathway.
Article
Environmental Sciences
Rishila Ghosh, Manushi Siddarth, Pawan Kumar Kare, Basu Dev Banerjee, Om Prakash Kalra, Ashok Kumar Tripathi
Summary: The study suggests that beta-endosulfan may be involved in the development of chronic kidney disease through oxidative stress and profibrotic signaling, leading to fibrotic changes in renal cells and possible onset of epithelial to mesenchymal transition.
ENVIRONMENTAL TOXICOLOGY
(2021)
Article
Medicine, General & Internal
Ali H. Abu Almaaty, Reham A. Elmasry, Mayada S. Farrag, Fayez Althobaiti, Adil Aldhahrani, Eman Fayad, Mona A. Hussain
Summary: The study showed that HUCB MNCs have a therapeutic effect against AKI and gentamicin-induced genotoxicity due to their regenerative property, significantly reducing renal damage and chromosomal aberrations.
FRONTIERS IN MEDICINE
(2021)
Article
Pharmacology & Pharmacy
Boxiang Zhang, Shiwen Liu, Yeqing Sun, Dan Xu
Summary: This study investigated the effects of the organochlorine pesticide endosulfan on human renal tubular epithelial cells, and found that it can induce kidney cell injury by modulating ACE2 through up-regulating miR-429. This provides new evidence for the pathogenesis of chronic kidney disease.
Article
Food Science & Technology
Enrique Garcia-Perez, Dojin Ryu, Hwa-Young Kim, Hae Dun Kim, Hyun Jung Lee
Summary: The study showed that OTA induced dose- and time-dependent cytotoxicity in both LLC-PK1 and HK-2 cells, with HK-2 cells being more sensitive to OTA toxicity. OTA caused significant oxidative stress in HK-2 cells compared to LLC-PK1 cells.
Article
Biochemistry & Molecular Biology
Keiko Hosohata, Denan Jin, Shinji Takai
Summary: In this study, the protective effect of Glaucocalxin A (GLA) on acute kidney injury (AKI) was investigated. GLA improved cell viability, suppressed reactive oxygen species (ROS) production, elevated superoxide dismutase activity, and prevented cell death in HK-2 human renal tubular epithelial cells exposed to hypoxia/reoxygenation (H/R). GLA also ameliorated the activation of the Akt/Nrf2/HO-1 signaling pathway. These findings suggest that GLA may serve as a promising therapeutic drug for AKI.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Immunology
Harzhin Hiwa Ali, Zheen Aorahman Ahmed, Tavag Ahmed Aziz
Summary: The present study evaluated the synergistic effects of telmisartan and quercetin in 5-FU induced nephrotoxicity in rats. The combination therapy significantly attenuated kidney injury and inflammation markers, as well as restored antioxidant capacity. The proposed mechanism could be the additive inhibitory effect on RAS provided by both telmisartan and quercetin.
JOURNAL OF INFLAMMATION RESEARCH
(2022)
Article
Food Science & Technology
Hui Chen, Lixing Cao, Kai Han, Han Zhang, Jinling Cui, Xuan Ma, Shuang Zhao, Chong Zhao, Shutao Yin, Lihong Fan, Hongbo Hu
Summary: This study found that Patulin (PAT) decreases the activity of SLC7A11, a core subunit of system x(c)(-), leading to a reduction in glutathione (GSH) synthesis. Additionally, PAT promotes the effects of the apoptotic inducer RSL3 in normal renal cells and exacerbates folic acid-induced nephrotoxicity in a mouse model of acute kidney injury.
FOOD AND CHEMICAL TOXICOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Hiroshi Kuwata, Yuki Tomitsuka, Emiko Yoda, Shuntaro Hara
Summary: In this study, ACSL4 knockdown was found to decrease the levels of PUFA-derived acyl-CoA, particularly leading to a decrease in DHA-containing phospholipids. This inhibition further suppressed cell death induced by ferroptosis.
BIOSCIENCE REPORTS
(2022)
Article
Water Resources
Swetha Palaniappan, Chakravarthy Marx Sadacharan, Bahman Rostama
Summary: This study found that the dose of microplastics has a negative correlation with cell viability, and affects cell metabolism and oxidative stress; Treatment with microplastics results in increased expression of superoxide dismutase-3 gene, as well as diverse mechanisms in regulating inflammatory responses.
EXPOSURE AND HEALTH
(2022)
Article
Urology & Nephrology
Shanshan Liu, Lishu Zhao, Li Zhang, Lujun Qiao, Shufang Gao
Summary: The increased expression of miR-574-5p in renal cell culture promotes cell viability, while septic patients with AKI show decreased expression of miR-574-5p. Serum miR-574-5p may serve as a novel biomarker for septic AKI.
Article
Chemistry, Medicinal
Jiri Handl, David Malinak, Jan Capek, Rudolf Andrys, Erika Rousarova, Martina Hauschke, Lenka Bruckova, Petr Cesla, Tomas Rousar, Kamil Musilek
Summary: This study found that certain double charged oximes have effects on dehydrogenase activity, while the effects of chlorinated oximes were deemed unreliable due to rapid degradation in the assay medium.
CHEMICAL RESEARCH IN TOXICOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Jan Capek, Tomas Rousar
Summary: The potential of nanomaterials in medicine and industry is vast, but their toxicity and impact on cellular pathways need to be thoroughly evaluated. Nanomaterials can induce the production of harmful reactive oxygen species in cells, while cells have various repair and antioxidant mechanisms to counteract this effect.
Article
Multidisciplinary Sciences
Majtnerova Pavlina, Capek Jan, Petira Filip, Handl Jiri, Rousar Tomas
Summary: A spectrofluorometric assay was developed to detect nuclear condensation and fragmentation in intact cells induced by various apoptotic inducers. This method showed similar sensitivity to the TUNEL assay but with the benefits of being fast, cost-effective, and high throughput. The spectrofluorometric assay could potentially become a routine method for characterizing cell death processes.
SCIENTIFIC REPORTS
(2021)
Correction
Multidisciplinary Sciences
Pavlina Majtnerova, Jan Capek, Filip Petira, Jiri Handl, Tomas Rousar
SCIENTIFIC REPORTS
(2021)
Article
Environmental Sciences
Katerina Fialova, Monika Motlochova, Lenka Cermakova, Katerina Novotna, Jana Bacova, Tomas Rousar, Jan Subrt, Martin Pivokonsky
Summary: This study investigated an alternative method of demanganization using a newly synthesized TiO2-based adsorbent. The adsorbent showed high effectiveness in removing Mn2+ due to its unique characteristics. The adsorption process involves electrostatic interactions and ion-exchange. The optimal pH for Mn2+ removal was found to be 7. Different isotherm models were selected based on the presence of different ions.
ENVIRONMENTAL TECHNOLOGY
(2023)
Article
Chemistry, Physical
Guru Karthikeyan Thirunavukkarasu, Jana Bacova, Olivier Monfort, Ewa Dworniczek, Emil Paluch, Muhammad Bilal Hanif, Sajid Rauf, Monika Motlochova, Jan Capek, Karol Hensel, Gustav Plesch, Grzegorz Chodaczek, Tomas Rousar, Martin Motola
Summary: This study compared two TiO2 nanopowders in terms of toxicity, photoinduced antimicrobial and antibiofilm properties, and photocatalytic activity. TiAP showed higher efficiency in photocatalysis and bacterial inactivation compared to P25.
APPLIED SURFACE SCIENCE
(2022)
Article
Biochemical Research Methods
Jana Vanova, David Malinak, Rudolf Andrys, Miroslav Kubat, Tomas Mikysek, Erika Rousarova, Kamil Musilek, Tomas Rousar, Petr Cesla
Summary: Acetaminophen overdose can cause acute liver injury, highlighting the need for a fast and convenient detection method. In this study, a gradient HPLC-UV/MS method was developed by synthesizing and characterizing the metabolites of acetaminophen, optimizing elution conditions, and evaluating the effects of mobile phase composition and additives on separation and detection. The method demonstrated effectiveness, sensitivity, and specificity in the analysis of human kidney HK-2 cell lysates, making it suitable for routine use in acetaminophen toxicity studies.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Food Science & Technology
Jiri Handl, Pavlina Nyvltova, Jan Capek, Petr Cesla, Anichka Hovsepyan, Sona Avetisyan, Petra Micankova, Lenka Bruckova, Pavla Stankova, Katerina Knotkova, Tigran Petrosyan, Tomas Rousar
Summary: The study compared the biological effects of bacterial melanin and synthetic melanin on neuroblastoma cells. The findings indicated that bacterial melanin caused less cell impairment and lower toxicity compared to synthetic melanin at similar dosages.
FOOD AND CHEMICAL TOXICOLOGY
(2022)
Article
Nanoscience & Nanotechnology
Jana Bacova, Petr Knotek, Katerina Kopecka, Ludek Hromadko, Jan Capek, Pavlina Nyvltova, Lenka Bruckova, Ladislava Schroterova, Blanka Sestakova, Jiri Palarcik, Martin Motola, Dana Cizkova, Ales Bezrouk, Jiri Handl, Zdenek Fiala, Emil Rudolf, Zuzana Bilkova, Jan M. Macak, Tomas Rousar
Summary: The study optimized the experimental conditions for preparing TiO2 P25 for toxicity testing in A549 cells. Results showed that the use of fetal bovine serum to maintain sufficient colloid stability of dispersed TiO2 P25 is crucial. Under these conditions, TiO2 P25 did not significantly impair A549 cells. When the defined parameters for using TiO2 P25 in A549 cells were met, similar biological effects results were obtained in two independent cell laboratories.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2022)
Article
Toxicology
Tomas Rousar, Jiri Handl, Jan Capek, Pavlina Nyvltova, Erika Rousarova, Miroslav Kubat, Lenka Smid, Jana Vanova, David Malinak, Kamil Musilek, Petr Cesla
Summary: Cysteine conjugates of acetaminophen and p-aminophenol induce harmful cellular damage in human kidney cells, including a decrease in glutathione levels and disappearance of mitochondrial membrane potential.
ARCHIVES OF TOXICOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Jana Bacova, Ludek Hromadko, Pavlina Nyvltova, Lenka Bruckova, Martin Motola, Roman Bulanek, Martina Rihova, Tomas Rousar, Jan M. Macak
Summary: This study provides the first comprehensive toxicity study of Al2O3, SiO2, ZrO2, TiO2, and WO3 fibers effects in cultured epithelial A549 cells. The tested fibers did not exhibit larger toxicity risk in human pulmonary A549 cells in comparison to nanoparticles of the same chemical composition, except for Al2O3 and TiO2 fibers causing significant cell damage.
ENVIRONMENTAL SCIENCE-NANO
(2022)
Article
Toxicology
Kathryn Page, Walter Westerink, Kristie Sullivan, Thomas McDonald, Clive Roper
Summary: This study developed a new method to assess the skin irritation of antimicrobial cleaning products. The method utilized a more human-like model and demonstrated its effectiveness through comparison with in vivo rabbit skin irritation data.
TOXICOLOGY IN VITRO
(2024)
Article
Toxicology
Arupam Patra, Thirukumaran Kandasamy, Siddhartha Sankar Ghosh, Gurvinder Kaur Saini
Summary: This study successfully produced recombinant anisoplin and demonstrated its significant anti-cancer effect and ability to induce apoptosis in breast cancer cells. The activation of related signaling pathways may be the key to cell death.
TOXICOLOGY IN VITRO
(2024)
Article
Toxicology
Thomas Haller, Alexander Jesacher, Alberto Hidalgo, Christina Schmidt
Summary: This study used live cell imaging to observe the accumulation of amiodarone in primary rat alveolar type II cells, and found that it specifically accumulates in lamellar bodies. The uptake is rapid, while storage is persistent. The main mechanisms for intracellular bioaccumulation of amiodarone are proposed to be passive diffusion, ion-trapping, and lipophilic interactions.
TOXICOLOGY IN VITRO
(2024)
Article
Toxicology
Filiz Bakar-Ates, Erva Ozkan
Summary: This study investigated the ferroptotic effect of CuB in breast cancer cells and evaluated its combination with erastin, a ferroptosis inducer. The results showed that the combination treatment significantly activated the ferroptotic pathways and altered the expression of iron-related proteins in breast cancer cells.
TOXICOLOGY IN VITRO
(2024)
Article
Toxicology
Rachel Z. Bennie, Ian C. Shaw
Summary: Estrogen mimics are synthetic and naturally occurring compounds that can interact with estrogen receptors in animals. In vitro transactivation reporter gene assay and in silico molecular modelling can be used to predict the mimicry of these compounds, reducing reliance on animal studies.
TOXICOLOGY IN VITRO
(2024)
Article
Toxicology
Sevda Isik, Semra Cicek
Summary: This study found that high doses of riboflavin can cause cytotoxicity in rainbow trout gonad cells and affect the transcriptional expressions of antioxidant enzymes and growth and reproductive genes, potentially leading to DNA damage and cell death.
TOXICOLOGY IN VITRO
(2024)
Article
Toxicology
Ying Wang, Jie Wu, Mengqi Wan, Dou Yang, Fasheng Liu, Kehao Li, Manxin Hu, Yuanyuan Tang, Huiqiang Lu, Shouhua Zhang, Yuanzhen Xiong
Summary: m-Cresol is commonly used as an intermediate for pesticides and other industrial applications. This study investigated the hepatotoxicity of m-cresol using zebrafish larvae and explored its molecular mechanisms. The results suggest that m-cresol may induce liver damage in zebrafish larvae through oxidative stress and cell apoptosis pathways.
TOXICOLOGY IN VITRO
(2024)
Article
Toxicology
Vincent J. Manna, Shannon Dwyer, Vanessa Pizutelli, Salvatore J. Caradonna
Summary: The widespread use of electronic cigarettes and the emergence of a new illness have raised concerns about the effects of e-cigarette vapor on respiratory tissues. Researchers have developed a simple device to mimic the response of human airway tissue after long-term exposure to e-cigarette vapor, and have identified differences in the effects of different vapor compositions on airway tissue.
TOXICOLOGY IN VITRO
(2024)