Article
Biochemistry & Molecular Biology
Meriem Hamdi, Karina Canon-Beltran, Rosane Mazzarella, Yulia N. Cajas, Claudia L. V. Leal, Alfonso Gutierrez-Adan, Encina M. Gonzalez, Juliano C. Da Silveira, Dimitrios Rizos
Summary: Extracellular vesicles (EVs) containing abundant miRNAs have been identified in the bovine reproductive tract, with potential roles in cell-to-cell communication and reproductive functions.
Article
Biochemistry & Molecular Biology
Sohyeon Moon, Ok-Hee Lee, Byeongseok Kim, Jinju Park, Semi Hwang, Siyoung Lee, Giwan Lee, Hyukjung Kim, Hyuk Song, Kwonho Hong, Jaejin Cho, Youngsok Choi
Summary: The expression of YAP in the mouse endometrium is periodic and regulated by hormones. YAP plays an important role in the regulation of uterine endometrium dynamics through Hippo signaling.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Anna Laitakari, Lingzhi Liu, Thomas M. Frimurer, Birgitte Holst
Summary: GPR39 is a widely expressed G-protein coupled receptor in various tissues, activated by changes in extracellular Zn2+ concentration. It plays important roles in physiological functions and in diseases such as inflammation, cardiovascular diseases, bone formation, male fertility, addiction, and cancer. Novel synthetic agonists have revealed new functions, presenting opportunities for drug development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Yiran Zhou, Han Yan, Wenjun Liu, Chengqing Hu, Yuan Zhou, Ruya Sun, Yida Tang, Chao Zheng, Jichun Yang, Qinghua Cui
Summary: This study provides a comprehensive transcriptomic landscape of female mice during different phases of the reproductive cycle, revealing the widespread regulation of neuro-functions, immune functions, and blood coagulation. The study also identifies key transcription factors and pathways involved in the estrous cycle. Moreover, the study demonstrates the significant impact of the estrous cycle on the cardiovascular system and highlights the enhanced efficacy of certain hypotensive drugs during the estrus phase. Overall, this research contributes valuable insights for investigating the reproductive cycle and precision medicine.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Yifan Xu, Anthony P. Barnes, Nabil J. Alkayed
Summary: This review examines the role of GPR39 as a zinc-responsive member of the ghrelin family of G protein-coupled receptors in regulating various neurovascular and neurologic functions. It also discusses its expression pattern in the central nervous system and its recognized role in neurovascular, neurological, and neuropsychiatric disorders.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biology
Yentel Mateo-Otero, Francisco Madrid-Gambin, Marc Llavanera, Alex Gomez-Gomez, Noemi Haro, Oscar J. Pozo, Marc Yeste
Summary: Using pig as a model, this study investigated the relationship between metabolic activity of sperm and their quality, function, and fertility outcomes. High-quality sperm were associated with higher levels of glycolysis-derived metabolites, and the metabolic status of sperm greatly influenced oocyte fertilisation and embryo development. Overall, this study demonstrates that the basal metabolic activity of sperm influences their function, extending beyond fertilisation.
COMMUNICATIONS BIOLOGY
(2023)
Article
Endocrinology & Metabolism
Klaudia Barabas, Gergely Kovacs, Viola Vertes, Erzsebet Kovesdi, Peter Faludi, Ildiko Udvaracz, Daniel Pham, Dora Reglodi, Istvan M. Abraham, Zsuzsanna Nagy
Summary: This study found that embryonic deletion of PACAP significantly alters the structure and function of the GnRH-kisspeptin neuronal network, leading to changes in fertility. Specifically, PACAP KO mice experienced altered estrous cycles with longer periods of diestrus and decreased time in estrus. Furthermore, the number of kisspeptin neurons decreased in the ARC region, while ERα expression levels showed differential changes in different brain regions.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Developmental Biology
Elle C. Roberson, Anna M. Battenhouse, Riddhiman K. Garge, Ngan Kim Tran, Edward M. Marcotte, John B. Wallingford
Summary: This study found significant gene expression differences along the anteroposterior axis of the mouse oviduct, but relatively few changes in gene expression across the estrous cycle. The gene expression variances along the anteroposterior axis included genes related to embryonic development. The researchers speculated that the stable transcriptional dynamics in the mouse oviduct across the estrous cycle may be influenced by its extremely rapid five-day cycle.
DEVELOPMENTAL BIOLOGY
(2021)
Article
Neurosciences
Weinan Zhao, Qing Li, Yu Ma, Zhiyong Wang, Bingqian Fan, Xiaojing Zhai, Mengfan Hu, Qing Wang, Moruo Zhang, Chunyan Zhang, Yixue Qin, Sha Sha, Zhonghao Gan, Fan Ye, Yihan Xia, Guangchao Zhang, Li Yang, Shiya Zou, Zheng Xu, Sunhui Xia, Yumei Yu, Mannan Abdul, Jun-Xia Yang, Jun-Li Cao, Fang Zhou, Hongxing Zhang
Summary: Research on psychiatry- and pain-related behaviors in humans show strong sex differences, but the use of female mice in preclinical studies is limited due to potential behavioral variations over the estrous cycle. However, a study on female C57BL/6J mice found that these behaviors remain relatively consistent throughout different phases of the estrous cycle, suggesting that the estrous cycle may not be a major contributor to behavioral variability in female mice.
FRONTIERS IN NEUROSCIENCE
(2021)
Article
Neurosciences
Cathryn A. Cutia, Leanna K. Leverton, Xiyu Ge, Rana Youssef, Lori T. Raetzman, Catherine A. Christian-Hinman
Summary: Clinical evidence suggests that there are differential outcomes of comorbid conditions in patients with temporal lobe epilepsy (TLE) based on the lateralization of the seizure focus. This study evaluated the effects of targeting left or right dorsal hippocampus in a model of TLE, and found that it produced different outcomes in granule cell dispersion, body weight gain, and reproductive endocrine dysfunction in female mice. The findings suggest that lateralization of the epileptogenic insult may contribute to similar reproductive endocrine outcomes.
EXPERIMENTAL NEUROLOGY
(2022)
Article
Biochemistry & Molecular Biology
Zhipeng Sun, Yufang Liu, Xiaoyun He, Ran Di, Xiangyu Wang, Chunhuan Ren, Zijun Zhang, Mingxing Chu
Summary: This study investigates the molecular regulation of goat fecundity in the oviducts at the proteome and transcriptome levels. The results reveal genes involved in gamete storage, maturation, fertilization, and early embryonic development, providing insights into the molecular mechanisms underlying fecundity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Neurosciences
Katarzyna Mlyniec
Summary: Zinc plays a crucial role in the pathophysiology and antidepressant response of depression, interacting with various neuropeptides like GPR39 and BDNF. Reduced zinc levels are associated with depression, and zinc-mediated signaling may influence depressive-like behavior.
CURRENT NEUROPHARMACOLOGY
(2021)
Article
Endocrinology & Metabolism
Naira da Silva Mansano, Henrique Rodrigues Vieira, Roberta Araujo-Lopes, Raphael Escorsim Szawka, Jose Donato Jr, Renata Frazao
Summary: This study found that acute fasting can modulate the activity of the HPG axis and has gender-specific effects on Kisspeptin neurons. In female mice, fasting reduces the levels of luteinizing hormone and prolactin, while increasing follicle-stimulating hormone levels. Fasting also reduces Kisspeptin gene expression. Additionally, GABAergic transmission and the effects of neuropeptide Y on ARHKisspeptin neurons show gender differences.
Article
Reproductive Biology
Andressa Gonsioroski, Daryl D. Meling, Liying Gao, Michael J. Plewa, Jodi A. Flaws
Summary: Exposure to Iodoacetic acid altered estrous cyclicity, ovarian gene expression, and estradiol levels in mice, leading to changes in apoptotic factors, cell cycle regulators, steroidogenic factors, estrogen receptors, and oxidative stress markers.
BIOLOGY OF REPRODUCTION
(2021)
Article
Biochemistry & Molecular Biology
Urszula Doboszewska, Katarzyna Socala, Mateusz Pierog, Dorota Nieoczym, Jan Sawicki, Malgorzata Szafarz, Kinga Gawel, Anna Rafalo-Ulinska, Adam Sajnog, Elzbieta Wyska, Camila V. Esguerra, Bernadeta Szewczyk, Marzena Mackowiak, Danuta Baralkiewicz, Katarzyna Mlyniec, Gabriel Nowak, Ireneusz Sowa, Piotr Wlaz
Summary: This study found that TC-G 1008 exacerbated seizures and epileptogenesis, but its mechanism may not be solely through activating the GPR39 receptor. Therefore, activating GPR39 may not be a viable therapeutic strategy for treating epilepsy.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Reproductive Biology
Haolin Li, Xiaomeng Pei, Hao Yu, Wei Wang, Dagan Mao
Summary: This study investigated the effects of APN/AdipoRon on autophagy and apoptosis in goat luteal cells, and found that autophagy and apoptosis play important roles in the structural regression of goat corpus luteum.
Article
Reproductive Biology
Yan-Qiu Wang, He-Xuan Qu, Yan-Wei Dong, Jia-Jia Qi, Hua-Kai Wei, Hao Sun, Hao Jiang, Jia-Bao Zhang, Bo-Xing Sun, Shuang Liang
Summary: This research reveals the crucial role of Ferroptosis Suppressor Protein 1 (FSP1) in regulating the development and quality of porcine early embryos. Inhibition of FSP1 impairs blastocyst formation, induces glutathione-independent ferroptosis, and leads to oxidative stress due to mitochondrial dysfunction.
Article
Reproductive Biology
Xiong Lai, Ruizhuo Liu, Mengyu Li, Yaochun Fan, Hongxia Li, Guotao Han, Ruijie Guo, Hairui Ma, Huimin Su, Wanjin Xing
Summary: This study identifies WDR54 as a protein involved in sperm-oocyte fertilization and shows that it interacts with IZUMO1 and JUNO. The formation of the JWIJ complex on the oocyte surface suggests its importance in sperm-oocyte adhesion and fusion. This discovery provides new insights into the mechanisms of mammalian sperm-oocyte adhesion and fusion.
Article
Reproductive Biology
S. Nandi, B. Sampath Kumar, P. S. P. Gupta, S. Mondal, V. Girish Kumar
Summary: This study examined the effects of plant bioactive compounds on the survival and growth parameters of ovine preantral follicles granulosa cells (GCs) and the expression of key developmental genes. The results showed that KAE, MYR and QUE had dose-dependent responses on the morphological and functional parameters of the cells, with KAE being the most potent in augmenting ovarian functions.
Article
Reproductive Biology
Francisco E. Martin-Cano, Gemma Gaitskell-Phillips, Laura Becerro-Rey, Eva da Silva, Javier Masot, Eloy Redondo, Antonio Silva-Rodriguez, Cristina Ortega-Ferrusola, Maria Cruz Gil, Fernando J. Pena
Summary: The presence of a more efficient glycolysis mechanism depending on pyruvate in stallion spermatozoa can counteract the detrimental effects of higher glucose concentrations. Incubating spermatozoa with 10 mM pyruvate in a modified Tyrode's media with 67 mM glucose showed increased motility and maintained viability. This improvement is attributed to the conversion of lactate to pyruvate and increased NAD(+) enhancing glycolysis efficiency.
Article
Reproductive Biology
Thanida Sananmuang, Denis Puthier, Catherine Nguyen, Kaj Chokeshaiusaha
Summary: Ongoing progress in mRNA-Sequencing technologies has contributed to the refinement of assisted reproductive technologies. This study utilized mRNA-Seq datasets of various mammalian species to analyze differential transcript usage (DTU) and identified ten cross-species DTU genes. Gene Ontology (GO) term enrichment analysis revealed the involvement of these genes in RNA and cell-cycle control mechanisms, suggesting their importance during oocyte maturation. Further exploration of these genes' transcript isoforms may lead to the discovery of novel markers for reproductive technologies.
Article
Reproductive Biology
Chenglei Song, Zhe Zhang, Yilin Wei, Yaqing Dou, Kunlong Qi, Xiuling Li, Feng Yang, Xinjian Li, Kejun Wang, Ruimin Qiao, Xuelei Han
Summary: In this study, the changes in protein levels in boar sperm with different liquid preservation abilities were analyzed. Key proteins related to sperm liquid preservation ability were identified. The findings provide new insights into the molecular mechanisms of boar sperm liquid preservation ability.
Article
Reproductive Biology
Genevieve M. VanWye, Emily G. Smith, Christine Spinka, Michael F. Smith, Matthew C. Lucy, Jordan M. Thomas
Summary: A series of experiments evaluated treatment schedules for controlling the estrous cycle in beef heifers. The results showed that the duration of progestin treatment and the timing of prostaglandin administration had an impact on estrus expression and pregnancy rates.
Article
Reproductive Biology
Hongwei Duan, Shuai Yang, Longfei Xiao, Shanshan Yang, Zhenxing Yan, Fang Wang, Xiaofei Ma, Lihong Zhang, Yong Zhang, Junjie Hu, Xingxu Zhao
Summary: This study first elucidated the mechanism by which melatonin regulates progesterone secretion in the corpus luteum of sheep, namely by promoting progesterone secretion through the regulation of autophagy. This has important implications for further exploring the role of melatonin in regulating sheep ovarian function.
Article
Reproductive Biology
Zhijie Zheng, Yingfang Guo, Yonghui Zheng, Haichong Wu
Summary: This study demonstrates that miR-92b plays a protective role in LTA-induced endometritis by targeting FZD10 and reducing the production of pro-inflammatory cytokines, thus alleviating uterine injury.
Article
Reproductive Biology
Areeg Almubarak, Sanghoon Lee, Il-Jeoung Yu, Yubyeol Jeon
Summary: This study found that Nobiletin (NOB) can promote the cryosurvival and embryo development of porcine sperm after in vitro fertilization (IVF). The addition of NOB improves sperm motility, acrosome integrity, viability, and mitochondrial membrane potential, reduces lipid peroxidation, and increases cleavage and blastocyst formation rates. Furthermore, NOB treatment upregulates the expression of genes related to pluripotency and cell differentiation in the resulting embryos.
Article
Reproductive Biology
Yuan Li, Lishu Li, Wenjie Xiong, Xing Duan, Huaming Xi
Summary: This study investigated the cytotoxicity and toxicological mechanisms of fluorochloridone (FLC) on cultured goat Sertoli cells. The results showed that FLC exposure induced oxidative stress, promoted apoptosis and autophagy, inhibited lysosomal biogenesis, and blocked autophagic flux in goat Sertoli cells. FLC also caused mitochondrial dysfunction and disturbed mitophagy, leading to cell apoptosis. The antioxidant N-acetyl-L-cysteine (NAC) reduced FLC-induced ROS accumulation and reversed the disorder of autophagy levels.
Article
Reproductive Biology
Gabriela Ramos Leal, Thais de Almeida Oliveira, Mariana Pedrosa de Paula Guimaraes, Lucas Francisco Leodido Correia, Erlandia Marcia Vasconcelos, Joanna Maria Goncalves Souza-Fabjan
Summary: This study investigated the time course of lipid accumulation during IVM and the effects of lipid modulators on cat oocytes. The results showed that adding lipid modulators can reduce lipid content and improve viability after cryopreservation.
Article
Reproductive Biology
Isabella Rio Feltrin, Amanda Guimaraes da Silva, Cecilia Constantino Rocha, Priscila Assis Ferraz, Paola Maria da Silva Rosa, Thiago Martins, Juliano Coelho da Silveira, Milena Lopes Oliveira, Mario Binelli, Guilherme Pugliesi, Claudia Maria Bertan Membrive
Summary: The study evaluated the effects of E2 treatment on Nelore heifers on day 15 of the estrous cycle, finding that E2 promoted an increase in PGFM concentrations and accelerated functional and structural luteolysis by upregulating the expression of PGR and OXTR. This suggests that within 3 hours after E2 stimulus, the expression of these receptors is associated with triggering luteolysis in cattle.
Article
Reproductive Biology
Aline Matos Arrais, Angelo Jose Burla Dias, Claudio Luiz Melo de Souza, Alinne Gloria Curcio, Marco Roberto Bourg de Mello
Summary: This study evaluated the effect of phospholipase C (PLC) on the capacitation of cryopreserved ovine semen. The results showed that the inhibitor U73122 can prevent premature capacitation and acrosome reaction induced by the freezing procedure, without affecting the kinetics and integrity of the sperm membranes.