Article
Computer Science, Artificial Intelligence
Camilo A. Ruiz-Beltran, Adrian Romero-Garces, Martin Gonzalez, Antonio Sanchez Pedraza, Juan A. Rodriguez-Fernandez, Antonio Bandera
Summary: This paper presents a hardware-based embedded solution for real-time eye detection. By redesigning the popular Viola-Jones method, highly parallel single-pass image processing is achieved. Experimental validation shows that the proposed solution achieves 100% accuracy in eye detection.
EXPERT SYSTEMS WITH APPLICATIONS
(2022)
Article
Nanoscience & Nanotechnology
Gaurav Sinsinbar, Sushanth Gudlur, Bo Liedberg
Summary: Recent advancements in microbial contamination detection methods have facilitated the development of on-site and point-of-care testing for food and water samples. This study presents a novel fluorescence-based OmptinPolythiophene assay for the rapid and reliable detection of Escherichia coli in contaminated water samples. The assay demonstrates a significant reduction in detection time, from 1 hour to just 10 minutes, and includes colorimetric sensing capability for easy visual interpretation.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Spectroscopy
Anahita Afsah-Sahebi, S. Shirin Shahangian, Hamidreza Khodajou-Masouleh, Reza H. Sajedi
Summary: A multicomponent transition metal dicalchogenide-based nanozyme was synthesized and characterized, exhibiting peroxidase-mimicking activity with a higher affinity for H2O2 substrate than natural peroxidase. This nanozyme was used to develop colorimetric sensing systems for leukocytosis-related patient identification and WBC count measurement, as well as for quality assessment of honey samples and detection of biologically significant analytes.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Food Science & Technology
Foteini Roumani, Saioa Gomez, Cristina Rodrigues, Jorge Barros-Velazquez, Alejandro Garrido-Maestu, Marta Prado
Summary: The study developed a panfungal LAMP assay for fast detection of spoilage fungi in fruit preparations. With newly designed primers and two different detection strategies, the method showed high sensitivity and substantial agreement with the reference method.
Article
Materials Science, Multidisciplinary
Zihang Yang, Jingyang Zhao, Chao Liang, Hanqing Jiang
Summary: This paper presents a novel epidermal UV sensor that combines new materials and design strategies to accurately measure UV intensity. The sensor offers high sensitivity and rapid response to UVR, and is also compatible with human skin and skin color specificity. By combining UV photochromic and absorptive materials, personalized UV epidermal stickers can be developed to achieve naked-eye quasi-quantitative monitoring of UV exposure while meeting different skin types' specific needs. Experimental demonstrations validate the high applicability of these wearable UV sensors for managing UVR impact in daily life.
ADVANCED MATERIALS TECHNOLOGIES
(2023)
Article
Computer Science, Information Systems
Christine Dewi, Rung-Ching Chen, Chun-Wei Chang, Shih-Hung Wu, Xiaoyi Jiang, Hui Yu
Summary: Drowsiness is a significant risk factor for road safety. In this research, a technique for identifying eye blinks in real-time video recordings from a car dashboard camera is presented.
Article
Biochemistry & Molecular Biology
Dandan Jiang, Minghao Zheng, Xiaofan Ma, Yingzhen Zhang, Shaohua Jiang, Juanhua Li, Chunmei Zhang, Kunming Liu, Liqing Li
Summary: A fluorescent and colorimetric poly(acrylamide)-based copolymer probe P(AAm-co-RBNCH) has been designed for selective detection of Fe3+ ions. The probe shows high sensitivity and selectivity for Fe3+ ions in the range of 100-200 μM. The hydrogel sensor exhibits good swelling properties and reversibility.
Article
Computer Science, Information Systems
Yadong Xie, Fan Li, Yue Wu, Song Yang, Yu Wang
Summary: Research on using acoustic sensors on smartphones to detect drowsy driving has led to the development of D-3-Guard, a real-time detection system with high accuracy in detecting drowsy driving actions and classifying drowsiness levels. This system was validated through experiments with five drivers in real driving environments.
IEEE TRANSACTIONS ON MOBILE COMPUTING
(2021)
Article
Chemistry, Analytical
Julia Niehues, Christopher McElroy, Alexander Croon, Jan Pietschmann, Martin Frettloeh, Florian Schroeper
Summary: Foodborne zoonotic pathogens have a significant impact on food safety, necessitating faster detection methods. The use of quorum sensing combined with a special biosensor allows for rapid detection of pathogens, enabling real-time monitoring and proving to be faster than traditional cultivation methods.
Article
Chemistry, Analytical
Karthikeyan Kandasamy, Miftakhul Jannatin, Yu-Chie Chen
Summary: A rapid detection method for bacteria on sample surfaces was developed based on the endogenous enzymatic reactions of E. coli and S. aureus. The presence of the bacteria can be visually confirmed through the appearance of blue color in the swab and the formation of air bubbles in the reagent solution after two-step enzymatic reactions.
Article
Engineering, Manufacturing
Rifat-E-Nur Hossain, Jerald Lewis, Arden L. Moore
Summary: Monitoring melt pool temperature in additive manufacturing processes is crucial for detecting various defects in real time, whether they are natural occurrences or as a result of malicious hacking. Using a multi-sensor approach, this study developed a statistical method to effectively identify temperature deviations caused by intentionally introduced defects. While demonstrating effective detection for multiple defect types and sizes, the approach also presents strengths and limitations, with potential for expansion through advanced data analysis techniques like machine learning.
ADDITIVE MANUFACTURING
(2021)
Article
Multidisciplinary Sciences
Joshua T. Atkinson, Lin Su, Xu Zhang, George N. Bennett, Jonathan J. Silberg, Caroline M. Ajo-Franklin
Summary: In this study, biosensors that produce electrical readouts with fast detection times were developed by combining synthetic biology and materials engineering. Escherichia coli were programmed to produce an electrical current in response to specific chemicals, allowing for rapid detection of thiosulfate and an endocrine disruptor. This new platform provides design rules for sensing various chemicals and offers a potential solution for safeguarding ecological and human health.
Article
Chemistry, Multidisciplinary
Paula M. Castillo, Francisco J. Fernandez-Acejo, Jose M. Carnerero, Rafael Prado-Gotor, Aila Jimenez-Ruiz
Summary: The study utilizes the stabilizing effect of lysozymes to develop a quick naked-eye test for detecting urinary lysozyme concentrations over 57.2 μg/mL. By calculating color difference parameters, the test can reliably provide a positive colorimetric response in less than 15 minutes, making it useful for detecting disorders such as leukemia-associated lysozymurias.
Article
Green & Sustainable Science & Technology
Wanyuan Tang, Fan He, Ali Kashif Bashir, Xun Shao, Yanfen Cheng, Keping Yu
Summary: This paper proposes a remote sensing image rotation object detection method based on dynamic position information Transformer to address the problems of low accuracy and slow detection speed in existing algorithms. The method improves detection accuracy by enhancing the cross-attention operation of the decoder and iteratively updating the position information of object queries. It also improves the network's robustness for remote sensing image object detection using an image pyramid data processing method and introduces a rotating IoU matching loss function for oriented object detection to improve the accuracy of matching predicted boxes to true boxes. Experimental results on DOTA and SSDD datasets show that the proposed algorithm achieves an average detection accuracy of 73.70% and 90.3%, respectively, effectively improving the average detection accuracy of Transformer-based rotating object detection algorithms in aerial remote sensing images and providing better real-time detection performance.
SUSTAINABLE ENERGY TECHNOLOGIES AND ASSESSMENTS
(2023)
Article
Environmental Sciences
Long Sun, Jie Chen, Dazheng Feng, Mengdao Xing
Summary: This paper proposes a parallel ensemble deep learning framework for multi-target detection in UAV videos, achieving a balance between detection accuracy and speed.
Editorial Material
Multidisciplinary Sciences
Lu Yin, Joseph Wang
NATIONAL SCIENCE REVIEW
(2023)
Article
Biochemical Research Methods
Cristina Munoz-San Martin, Ana Montero-Calle, Maria Garranzo-Asensio, Maria Gamella, Victor Perez-Gines, Maria Pedrero, Jose M. Pingarron, Rodrigo Barderas, Noemi de-los-Santos-Alvarez, Maria Jesus Lobo-Castanon, Susana Campuzano
Summary: This work presents the first bioelectronic immunoplatforms for determining glycosylated haptoglobin (Hp) and both total and glycosylated Hp simultaneously. The immunoplatforms use non-competitive bioassays and magnetic microcarriers labeled with horseradish peroxidase (HRP) for detection. The method allows accurate determination of total and glycosylated Hp in colorectal cancer (CRC) cells, which is not possible with commercial ELISA kits and Western blot methodology due to lack of sensitivity.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Biophysics
Naveen K. Singh, Saeromi Chung, An-Yi Chang, Joseph Wang, Drew A. Hall
Summary: Stress is a common part of life and can be worsened by traumatic events. Chronic stress has negative effects on health and can be life-threatening. To address this issue, researchers have developed a wearable biomolecular sensor that can monitor cortisol levels in sweat.
BIOSENSORS & BIOELECTRONICS
(2023)
Review
Chemistry, Analytical
Esther Sanchez-Tirado, Lourdes Agui, Araceli Gonzalez-Cortes, Susana Campuzano, Paloma Yanez-Sedeno, Jose Manuel Pingarron
Summary: The study of the human microbiome is a multidisciplinary field that holds great potential for improving disease diagnosis and monitoring using microbiota biomarkers. Electrochemical biosensors, with their high sensitivity and fast response, combined with advancements in nanomaterials, offer unique tools for understanding the human microbiome and driving future developments. This review article focuses on the application of electrochemical (bio)sensors for monitoring microbial metabolites and disease biomarkers in the gastrointestinal microbiome, discussing challenges and future directions.
Article
Chemistry, Multidisciplinary
Rodolfo Mundaca-Uribe, Maya Holay, Amal Abbas, Nelly Askarinam, Janna Sofia Sage-Sepulveda, Luke Kubiatowicz, Ronnie H. Fang, Liangfang Zhang, Joseph Wang
Summary: Type 2 diabetes mellitus (T2DM) is characterized by high blood glucose levels due to insulin resistance, and metformin is the most commonly prescribed oral drug for lowering blood glucose levels in T2DM patients. However, its absorption and bioavailability are poor. This study introduces magnesium-based microstirrers to a metformin-containing pill matrix to enhance the glucose-lowering effect of metformin.
Article
Chemistry, Analytical
Beatriz Arevalo, Veronica Serafin, Maria Garranzo-Asensio, Ana Montero-Calle, Rodrigo Barderas, Paloma Yanez-Sedeno, Susana Campuzano, Jose M. Pingarron
Summary: This study presents the first amperometric biosensor capable of simultaneously determining the single or total content of various human immunoglobulin isotypes of anti-dsDNA antibodies, which are important biomarkers in autoimmune and neurodegenerative diseases. The biosensor involves the use of functionalized magnetic microparticles and specific secondary antibodies for detection. Clinical applications of the biosensor were successful in determining hIgs classes in healthy individuals, as well as patients with SLE and AD, yielding results consistent with established detection methods.
ANALYTICA CHIMICA ACTA
(2023)
Review
Chemistry, Multidisciplinary
Tamoghna Saha, Rafael Del Cano, Kuldeep Mahato, Ernesto De la Paz, Chuanrui Chen, Shichao Ding, Lu Yin, Joseph Wang
Summary: With the increasing diabetic population, the demand for glucose sensing devices has risen. Electrochemical biosensors show great potential for real-time glucose monitoring. Wearable devices have opened opportunities for noninvasive glucose monitoring. This review provides a comprehensive report on the status and potential of wearable electrochemical sensors for on-body glucose monitoring.
Article
Endocrinology & Metabolism
Eva Vargas, Ponnusamy Nandhakumar, Shichao Ding, Tamoghna Saha, Joseph Wang
Summary: Technologies and devices capable of frequent and real-time insulin measurements are urgently needed for patients with diabetes to guide optimal insulin dosing. This Perspective discusses the progress and challenges in moving insulin assays from laboratory-based assays to decentralized settings. The article explores promising technologies for insulin testing, such as disposable test strips, mobile systems, and wearable real-time insulin-sensing devices, as well as potential future developments for continuous insulin monitoring and integrated multisensor-guided closed-loop artificial pancreas systems.
NATURE REVIEWS ENDOCRINOLOGY
(2023)
Article
Oncology
Ana Montero-Calle, Maria Garranzo-Asensio, Rebeca M. Torrente-Rodriguez, Victor Ruiz-Valdepenas Montiel, Carmen Poves, Jana Dziakova, Rodrigo Sanz, Cristina Diaz del Arco, Jose Manuel Pingarron, Maria Jesus Fernandez-Acenero, Susana Campuzano, Rodrigo Barderas
Summary: The study aimed to assess whether p53 and p63 proteoforms derived from alternative splicing could have a differential seroreactivity and higher diagnostic value than canonical proteins in colorectal cancer. The researchers demonstrated the appearance of a differential seroreactivity among the proteoforms in colorectal cancer patients. These findings reveal increased complexity of the humoral immune response in cancer and highlight the potential for developing a blood-based biomarker signature for colorectal cancer detection.
Review
Chemistry, Multidisciplinary
Fangyu Zhang, Zhengxing Li, Chuanrui Chen, Hao Luan, Ronnie H. Fang, Liangfang Zhang, Joseph Wang
Summary: This review focuses on the integration of microorganisms and artificial components to create biohybrid microrobots, with a specific emphasis on microalgae-based systems. The distinct propulsion and phototaxis behaviors of microalgae, as well as methods for functionalizing their surface, are discussed. Various applications of these biohybrid microrobots in biomedical and environmental fields are presented, highlighting their advantages and challenges.
ADVANCED MATERIALS
(2023)
Review
Biochemical Research Methods
Susana Campuzano, Rodrigo Barderas, Maria Teresa Moreno-Casbas, Angeles Almeida, Jose M. Pingarron
Summary: In the era of personalization, personalized management of medicine and nutrition is crucial for life expectancy and quality of life. Implementing precision medicine and nutrition is a complex challenge that requires new technologies to meet cost, simplicity, and versatility requirements, as well as accurately determining molecular markers in biofluids at different omics levels almost in real time. This review critically discusses recent advances and positions electrochemical bioplatforms as suitable tools for advanced diagnostics, therapy, and precision nutrition.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Chuanrui Chen, Shichao Ding, Joseph Wang
Summary: Growing life expectancy presents challenges for strained health systems. Digital technologies have the potential to transform geriatric care and enable remote monitoring, promoting health and independence in aging populations. This Perspective summarizes the progress, challenges, and future opportunities of digital technologies in geriatric care, with a focus on wearables for health monitoring and maintaining independence at home.
Review
Chemistry, Analytical
Esther Sanchez-Tirado, Paloma Yanez-Sedeno, Jose Manuel Pingarron
Summary: Natural enzymes are used as special reagents in the preparation of electrochemical (bio)sensors, but their practical applications are limited due to certain drawbacks. Artificial enzymes based on nanomaterials, known as nanozymes, have been developed as an alternative, providing enhanced stability, low cost, easy production, and rapid activity. Among nanozymes, carbon-based nanozymes have been widely used in the preparation of electrochemical (bio)sensors due to their stable activity, tunability, and good biocompatibility.
Review
Chemistry, Multidisciplinary
Susana Campuzano, Jose M. Pingarron
Summary: Electrochemical affinity biosensors are rapidly evolving and becoming increasingly important in our daily lives. They combine their intrinsic attributes with advances in technology to offer a wide range of opportunities and applications. This perspective provides an updated and critical view of these biosensors and discusses the challenges and future directions they face.
Article
Biochemistry & Molecular Biology
Sandra Tejerina-Miranda, Maria Pedrero, Marina Blazquez-Garcia, Veronica Serafin, Ana Montero-Calle, Maria Garranzo-Asensio, A. Julio Reviejo, Jose M. Pingarron, Rodrigo Barderas, Susana Campuzano
Summary: This study developed the first electrochemical bioplatform for determining human endostatin, which shows high sensitivity and selectivity for diagnosing tumors and identifying patients in different stages.
BIOELECTROCHEMISTRY
(2024)
Article
Chemistry, Analytical
Qian Wu, Li Tian, Xiangyu Shan, Huiling Li, Chao Li, Juan Lu
Summary: A novel ECL sensor was developed by modifying Fe3CuO4-Ru(bpy)3+ and GO-CdS@ZnS on the electrode for the detection of permethrin. The sensor exhibited high sensitivity and a wide detection range.
Article
Chemistry, Analytical
Tingting Gong, Lei Liao, Bingying Jiang, Ruo Yuan, Yun Xiang
Summary: In this study, a fluorescent biosensing system for high sensitivity detection of MT was established using a new Ag+-stabilized DNA triplex probe (Ag+-SDTP) combined with catalytic hairpin assembly (CHA) and CRISPR/Cas12a signal enhancements. The sensing method is able to selectively detect MT from other non-specific molecules and can achieve low level detection of MT in diluted human serums, demonstrating its potential for monitoring disease-specific MT biomarker at trace levels.
Article
Chemistry, Analytical
Estrella Sanz Rodriguez, Kyra Wood, Paul R. Haddad, Brett Paull
Summary: This study presents a simple, rapid, and reliable method for determining boron in aqueous solutions using suppressed ion chromatography coupled to electrospray ionisation-triple quadrupole mass spectrometry. The method achieved a low limit of detection and demonstrated excellent analytical performance.
Article
Chemistry, Analytical
Aline Cournut, Paul Moustiez, Yannick Coffinier, Christine Enjalbal, Claudia Bich
Summary: Alzheimer's disease is a major cause of senile dementia and is characterized by the aggregation of neurofibrillary tangles and amyloid plaques in the brain. The A beta 1-42 peptide is considered the primary cause of neurotoxicity due to its insolubility and subsequent aggregation. A beta 1-42 and A beta 1-40 have been identified as relevant biomarkers for AD diagnosis, and SurfaceAssisted Laser Desorption/Ionization Mass Spectrometry (SALDI-MS) was used to monitor the A beta 1-42/A beta 1-40 ratio without prior sample treatment.
Article
Chemistry, Analytical
Dotse Selali Chormey, Buse Tugba Zaman, Tulay Borahan Kustanto, Sezin Erarpat Bodur, Suleyman Bodur, Elif Ozturk Er, Sezgin Bakirdere
Summary: This review provides an overview of the harmful effects of endocrine disrupting chemicals (EDCs) on human health and introduces the methods for analyzing EDCs. Recent research has focused on using green chemicals, such as deep eutectic solvents (DESs), for microextraction to ensure environmental safety.
Article
Chemistry, Analytical
Yan Zhang, Renfeng Xu, Jingjing Wu, Zhenghong Zhang, Yuhuang Wang, Hongqin Yang, Sheng Zhang
Summary: This study visualized the pore-like structures in prostate cancer cell membranes after treatment with the anticancer drug paclitaxel, demonstrating its mechanism of action related to oxidative damage.
Article
Chemistry, Analytical
Chihong Ma, Qin Zhou, Jinjin Shi, Hua Gao, Di Huang, Huimin Xue, Han Wang, Zhenzhong Zhang, Sen Yang, Junli Zhang, Kaixiang Zhang
Summary: We developed a CRISPR-empowered electrochemical biosensor, named PER-E-CRISPR, for amplification-free and sensitive detection of miR-21. The biosensor combines the advantages of CRISPR/Cas13a and primer exchange reaction (PER) for target amplification. Under optimized conditions, the PER-E-CRISPR assay exhibits a linear range from 10-13 to 10-7 M and a limit of detection of 30.2 fM. The biosensor shows excellent performance in actual plasma, indicating promising prospects for miRNA detection in molecular diagnosis.
Article
Chemistry, Analytical
Gang Liang, Jie Zhao, Yufei Gao, Tao Xie, Jianhui Zhen, Ligang Pan, Wenwen Gong
Summary: Molecular docking (MD) analysis is widely used for studying the interaction between aptamers and small molecules. This study established steady-state aptamers of tertiary structures (SATS) using specific aptamers of tetracycline antibiotics as docking models. Molecular docking results revealed multiple binding sites in the SATS of aptamers, with significant variations in binding free energy (BFE) and docking score (DS). The study also proposed a method for MD analysis based on SATS, providing insights into binding mode and predicting binding sites.
Article
Chemistry, Analytical
Yixuan Xu, Changchang Teng, Huiping Dang, Dalong Yin, Lifeng Yan
Summary: Stable organic radical molecules with high NIR fluorescence quantum efficiency have been synthesized and encapsulated into nanoparticles, which show great potential for both photodynamic therapy (PDT) and photothermal therapy (PTT) of tumors.
Article
Chemistry, Analytical
Yu Su, Dandan Yang, Yanjie Wang, Jie Ding, Lan Ding, Daqian Song
Summary: In this study, a highly selective molecularly imprinted polymer Cu-MIPs@CS was designed and synthesized for bisphenol A (BPA). Cu-MIPs@CS showed significantly enhanced imprinting factor and selective factor towards BPA compared to MIPs@CS. It also exhibited superior discrimination ability between BPA and its structural analogue. Additionally, Cu-MIPs@CS were successfully applied as a solid phase extraction adsorbent for the determination of BPA in drinking water samples.
Article
Chemistry, Analytical
Yi Qiao, Qiongdan Zhang, Yukun He, Tianguang Cheng, Jing Tu
Summary: Single cell heterogeneity plays an important role in many biological phenomena, and distinguishing cells with specific mutations is beneficial for clinical diagnosis and drug screening. This study introduces a gel plate platform that can distinguish cell types based on their phenotypes, with low equipment requirement. The gel platform has potential in point-of-care circumstances and single-cell stimulation responses that prioritize efficiency and simplicity.
Article
Chemistry, Analytical
Chengke Wang, Shuyang Sun, Ping Wang, Huawei Zhao, Wenling Li
Summary: The food safety of aquatic products is a global concern. Nanotechnology-based analyses have advantages in the detection of bacteria, metal ions, and small molecule contaminants. This review summarizes the recent advances in biosensing strategies for aquatic products and highlights the application of nanomaterials, lateral flow-based biosensors, surface-enhanced Raman scattering, microfluidic chips, and molecular imprinting technologies.
Article
Chemistry, Analytical
Liping Zhu, Zeng Tang, Xuemei Zhang, Li Zhu, Tian Meng, Linying Yu, Ting Xiao, Shasha Lu, Xiaoli Xiong, Xiurong Yang
Summary: A sensitive off-on electrochemiluminescence DNA sensor was developed using Exo III-assisted cascade amplification system. The sensor achieved high selectivity and ultra-high sensitivity in detecting target DNA by utilizing ECL and SPR effects. This study provides a new perspective for designing highly sensitive and programmable ECL biosensors.
Article
Chemistry, Analytical
Tong Li, Mengxu Sun, Suping Xia, Ting Huang, Rong-Tian Li, Chunrong Li, Zong Dai, Jin-Xiang Chen, Jun Chen, Nuan Jia
Summary: Here, we developed a binary system based on DNA tetrahedron and fluorogenic RNA aptamers for highly specific and label-free mRNA imaging in living cells. This system achieved high specificity label-free detection of nucleic acids with a detection limit of 1.34 nM. The method has potential applications in early disease diagnosis and new drug development.
Article
Chemistry, Analytical
Yao Zhao, Shu Zhang, Wang Yao, Yuxuan Zhu, Jing Qian, Juan Yang, Nianjun Yang
Summary: A new hierarchical nanostructured composite with high conductivity and catalytic activity is synthesized. The sensor exhibits significant catalytic effect and high sensitivity for monitoring the environmental endocrine disruptor bisphenol A (BPA).
