Article
Critical Care Medicine
Luis Felipe Reyes, Esteban Garcia, Elsa D. Ibanez-Prada, Cristian C. Serrano-Mayorga, Yuli V. Fuentes, Alejandro Rodriguez, Gerard Moreno, Alirio Bastidas, Josep Gomez, Angelica Gonzalez, Christopher R. Frei, Leo Anthony Celi, Ignacio Martin-Loeches, Grant Waterer
Summary: This study aimed to determine the impact of macrolide-based therapy on long-term mortality in patients with community-acquired pneumonia admitted to the ICU. The results showed that macrolide-based treatment can significantly reduce the risk of long-term mortality in these patients.
Review
Infectious Diseases
Sang -Ho Choi, Antoni Cesar, Timothy Arthur Chandos Snow, Naveed Saleem, Nishkantha Arulkumaran, Mervyn Singer
Summary: For hospitalized adults with mild-to-moderate community-acquired pneumonia (CAP), respiratory fluoroquinolone monotherapy or β-lactam plus macrolide combination therapy are suggested as first-line options according to guidelines. However, the efficacy of these regimens has not been adequately evaluated.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2023)
Article
Infectious Diseases
Lauren Freeman, Ashley Milkovits, Lauren McDaniel, Nathan Everson
Summary: This study compared the rates of treatment modifications and failures between A + C therapy and A + G therapy for Enterococcus faecalis infective endocarditis (EFIE). The results showed that A + C therapy had lower rates of treatment modifications and adverse events, with the most common adverse event being nephrotoxicity, while the A + G therapy had a higher incidence of acute kidney injury.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2022)
Article
Environmental Sciences
Ming Zheng, Suwen Tang, Yangyang Bao, Kevin D. Daniels, Zuo Tong How, Mohamed Gamal El-Din, Jie Wang, Liang Tang
Summary: A fully automated SPE coupled with UHPLC-MS/MS method was developed for analysis of antibiotics with optimized sample preparation and instrument parameters. This method can meet the needs of trace antibiotic residues detection in waters with high sensitivity and precision.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Food Science & Technology
Durmus Sert, Emin Mercan
Summary: The study found that ozone treatment can significantly reduce potentially harmful substances in milk concentrate and whey concentrate, improving the physicochemical properties of the samples. Ozone-treated samples had softer texture, higher lightness, lower yellowness, and lower microbial load.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Environmental Sciences
Ievgeniia Prekrasna, Mariia Pavlovska, Artem Dzhulai, Evgen Dykyi, Nikiforos Alygizakis, Jaroslav Slobodnik
Summary: A large-scale study in the Black Sea reveals an alarming antibiotic resistance problem, calling for regular monitoring of antibiotic resistance gene abundance in the region.
FRONTIERS IN ENVIRONMENTAL SCIENCE
(2022)
Article
Microbiology
Mohamad Maatouk, Ahmad Ibrahim, Jean-Marc Rolain, Vicky Merhej, Fadi Bittar
Summary: Candidate Phyla Radiation (CPR) microbes have been identified as a new branch in the tree of life, representing over 26% of microbial diversity. Despite their small size, reduced genomes, and reliance on symbiotic relationships with bacterial hosts, CPR microbes are abundant in various environments. They possess a diverse range of antibiotic resistance genes and are active participants in microbial competition, equipped with mechanisms for combat in different habitats.
Article
Engineering, Environmental
Natalia Ormeno-Cano, Jelena Radjenovic
Summary: The graphene sponge electrodes doped with atomic boron and nitrogen showed significant effectiveness in the electrochemical degradation of antibiotics. The removal efficiency varied for different antibiotics at different electrolyte flow rates, influenced by factors such as gas bubble formation.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Biochemistry & Molecular Biology
Rasel Khan Manik, Zimam Mahmud, Israt Dilruba Mishu, Md Sourav Hossen, Zakir Hossain Howlader, A. H. M. Nurun Nabi
Summary: This study provides important insights into the prevalence and mechanisms of antibiotic resistance in hospital wastewater isolates. The results showed that resistance to multiple antibiotic classes was common among the isolates, and the most common resistance genes were blaTEM, blaCTX-M1, and blaNDM. Polymyxin was found to be the most effective antibiotic against wastewater isolates.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2023)
Article
Microbiology
Hussein Chalhoub, Stefanie Kampmeier, Barbara C. Kahl, Francoise Van Bambeke
Summary: This study investigates the impact of efflux and target mutations on the drug resistance of Achromobacter to multiple drugs and finds that high efflux activity is associated with elevated MICs for amikacin or azithromycin.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Chemistry, Medicinal
Xie-Peng Liu, Wei Lv, Fei Zhao, Jing Ding, Jue-Ru Zhang, Feng Xue, Jian-Zhong Zhang, Li-Yong Liu, Mark Cushman, Yun Li, Jian-Hua Liang
Summary: The hybrids of macrolides and quinolones bridged with linkers from 11,12-cyclic carbamate of macrolides showed different structure-activity relationships and improved metabolic stability compared to telithromycin. The optimized macrolone was 38-fold more potent than telithromycin against A2058/2059-mutated Mycoplasma pneumoniae, potentially due to a novel mode of action between the carboxylic acid of quinolone moiety and the bacterial ribosome.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2022)
Article
Environmental Sciences
Claudia Sanz, Marta Casadoi, Dorde Tadic, Edward J. Pastor-Lopez, Laia Navarro-Martin, Joan Parera, Jordi Tugues, Carlos A. Ortiz, Josep M. Bayona, Benjamin Pina
Summary: The study found that organic soil amendments have a certain impact on both ARG loads and microbiomes in soil samples, especially after the second harvesting cycle. The slurry solid fraction had the largest impact on both ARG loads and microbiome variation, while its digestion derivatives had a smaller impact.
ENVIRONMENTAL RESEARCH
(2022)
Article
Spectroscopy
Ye Jiang, Xiaochan Wang, Guo Zhao, Yinyan Shi, Yao Wu
Summary: The study demonstrates the use of multifunctional Fe3O4@mTiO2@Ag core-shell nanoparticles as enhanced substrates for in-situ surface-enhanced Raman spectroscopy (SERS) detection of quinolone antibiotics in aqueous environments. The nanoparticles achieved a detection limit of 1 x 10-9 mol/L for five antibiotics and 1 x 10-8 mol/L for one antibiotic. There was a quantitative relationship between antibiotic concentrations and SERS peak intensities, and the nanoparticles also assisted in the photocatalytic degradation of antibiotics in water.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Engineering, Environmental
Amine Ezzariai, Eric Pinelli, Loubna El Fels, Georges Merlina, David Riboul, Ahmed Khadra, Maialen Barret, Julie Jimenez, Marlene Z. Lacroix, Lamfeddal Kouisni, Alain Bousquet-Melou, Dominique Patureau, Mohamed Hafidi
Summary: This study investigated the fate of antibiotics during composting and its relationship with organic matter fractionation. The results showed that sludge composting effectively removed most antibiotics, but some antibiotics persisted in the final compost. The study also found that the concentration of antibiotics affected the behavior of organic fractions, and different antibiotics had different effects on different plants.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2022)
Review
Infectious Diseases
Sabrina Lunara Santos Pavelquesi, Ana Carolina Almeida de Oliveira Ferreira, Angeislenie Ricelle Magalhaes Rodrigues, Calliandra Maria de Souza Silva, Daniela Castilho Orsi, Izabel Cristina Rodrigues da Silva
Summary: The study revealed high resistance rates of Salmonella to tetracyclines and sulfonamides, with poultry and pork being the most frequent samples used to isolate Salmonella. The most frequently detected tetracycline resistance genes in Salmonella were tetA and tetB, while sul1 was the most common sulfonamide resistance gene. These genes are associated with plasmids, transposons, or both, and have potential for transfer to other bacteria, environments, animals, and humans.
Article
Food Science & Technology
Caroline Liboreiro Paiva, Dea A. M. Netto, Valeria A. V. Queiroz, Maria Beatriz A. Gloria
Summary: This study quantified the levels of free bioactive amines during germination of tannin and tannin-free sorghum seeds and seedlings. It was found that tannin-free sorghum seeds had higher levels of spermidine, spermine, putrescine, and total amines compared to tannin sorghum. Throughout germination, putrescine was prevalent followed by spermidine. Spermine was only detected in the 7th germination day in tannin sorghum. The findings suggest that by selecting different genotypes, the polyamine contents in germinated sorghum can be modulated for specific food applications.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Pharmacology & Pharmacy
Matheus Augusto de Castro, Pedro Henrique Reis, Christian Fernandes, Ricardo Geraldo de Sousa, Thomas Toshio Inoue, Silvia Ligorio Fiaiho, Armando Silva-Cunha
Summary: Ocular chemical burns can be effectively treated with a poloxamer-based thermoresponsive in-situ gelling system containing hyaluronic acid and indomethacin. The optimized system (F2) has a gelling temperature of 34.2 degrees C and shows improved wound healing in the ocular surface.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Food Science & Technology
Paulo Tulio de Souza Silveira, Maria Beatriz Abreu Gloria, Isabela Portelinha Tonin, Marina Oliveira Paraiso Martins, Priscilla Efraim
Summary: The presence and levels of bioactive amines during the processing of cocoa were investigated in this study. The fermentation time, pulp content, and cocoa variety were found to influence the content of bioactive amines. Cadaverine was detected during drying in all cocoa varieties, reaching levels of up to 17.96 mg/kg dwb. Most bioactive amines decreased during roasting, except for phenylethylamine, which increased.
Article
Pharmacology & Pharmacy
Gabriel Silva Marques Borges, Pierre Sicard, Cristina de Mello Gomides Loures, Fernanda Gontijo Cristina Evangelista, Camila Campos Sales, Adriano de Paula Sabino, Christian Fernandes, Lucas Antonio Miranda Ferreira, Sylvain Richard
Summary: All-trans retinoic acid and arsenic trioxide are effective treatments for acute promyelocytic leukemia, but they have certain drawbacks such as patient relapse and cardiotoxicity. In this study, a self-nanoemulsifying drug delivery system enriched with tocotrienols was developed to enhance the cytotoxicity of all-trans retinoic acid and mitigate the cardiotoxic effects of arsenic trioxide. The system showed promising results in improving the antileukemic properties of all-trans retinoic acid, but it also influenced the electrocardiogram changes caused by arsenic trioxide administration.
Article
Chemistry, Medicinal
Maria Betania de Freitas-Marques, Talita Santos do Valle, Barbara Caroline Rodrigues de Araujo, Rita de Cassia de Oliveira Sebastiao, Wagner da Nova Mussel, Maria Irene Yoshida, Christian Fernandes
Summary: This study evaluated the thermal energy and tableting effects of benznidazole (BZN), excipients, and tablets using techniques such as infrared spectroscopy, X-ray diffraction, and thermal analysis. The results revealed the dehydration of talc and alpha-lactose monohydrate and the conversion of beta-lactose to stable alpha-lactose during tableting. The findings highlight the importance of considering thermal energy and tableting effects in the manufacturing of BZN.
DRUG DEVELOPMENT AND INDUSTRIAL PHARMACY
(2023)
Article
Chemistry, Applied
Geisiane Santos Silva, Bruno M. Dala-Paula, Eliete S. Bispo, Maria Beatriz A. Gloria
Summary: The influence of under-fermented cocoa on bioactive amines in chocolate and their in vitro bioaccessibility was investigated. Adding different proportions of under-fermented cocoa during chocolate production can modulate the levels and bioaccessibility of amines.
Article
Chemistry, Applied
D. C. S. da Silva, F. B. de Carvalho-Guimaraes, I. P. Valente, N. Cunha, S. C. da C. Sanches, D. A. da Silva, J. O. C. Silva Junior, L. H. M. da Silva, A. M. da C. Rodrigues
Summary: This study evaluated the fatty acid composition, nutritional quality, FTIR spectra, and rheological behavior of blends of annatto seed oil (ASO) and cupuassu seed fat (CSF) at different ratios (30:70, 50:50, and 70:30 % w/w). The results showed that ASO increased the content of polyunsaturated fatty acids in the blends, while CSF conferred higher contents of monounsaturated fatty acids. The blends exhibited low atherogenicity and thrombogenicity indices, indicating their nutritional advantages. The Newtonian fluid behavior and FTIR results suggested that mixing ASO and CSF at different proportions did not affect the functional groups. ASO showed higher activation energy value, indicating its viscosity was more affected by temperature changes. The Newtonian model proved suitable for describing the behavior of the samples.
Article
Biochemical Research Methods
Melina Luiza Vieira Diniz, Jessica Mauricio Batista, Pedro Henrique Reis da Silva, Christian Fernandes
Summary: This study aimed to develop and validate a MEPS coupled to high performance liquid chromatography method for the analysis of benznidazole in human plasma. The method was proved to be selective, precise, accurate, robust and linear in the range from 0.5 to 6.0 μg mL-1, and it was applied successfully to assess this drug in plasma samples.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2023)
Article
Food Science & Technology
Dayala Albuquerque da Silva, Antonio Manoel da Cruz Rodrigues, Adenilson Oliveira dos Santos, Rebeca Salvador-Reyes, Luiza Helena Meller da Silva
Summary: This study characterized blends of pracaxi oil/cupuassu fat and pracaxi oil/palm stearin in different proportions, and found that enzymatic interesterification improved the texture and stability of the blends.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Ophthalmology
Rodrigo Jorge, Isabela Franco Villela, Christian Fernandes, Thais Marino de Azevedo Bastos, Ingrid U. Scott, Armando da Silva Cunha, Silvia Ligorio Fialho, Pedro Henrique Reis da Silva, Andre Messias
Summary: This article reports a case of pseudophakic cystoid macular edema (PCME) in which conventional treatment was ineffective but improved after intravitreal acetazolamide implant injection. Complete resolution of symptoms was observed 4 weeks after the injection. Further investigation is needed to confirm these findings.
DOCUMENTA OPHTHALMOLOGICA
(2023)
Article
Chemistry, Applied
Roberto Cesar Santos de Sousa, Maria Beatriz Abreu Gloria
Summary: Brazil is facing a growing problem of overweight and obesity, and various measures have been taken to address this issue, such as reducing sugar intake and implementing new packaging labeling. As a result, the use of low or non-caloric sweeteners (LNCS) has increased. This study examined the presence of LNCS in six food groups in Brazil, and found that 17.7% of the included products contained at least one LNCS, with an average of 2.21 LNCS per sweetened product. Non-alcoholic beverages and dairy products had the highest occurrence of LNCS.
FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT
(2023)
Article
Food Science & Technology
Douglas Evangelista Braga, Rafael von Sperling de Souza, Fabiano Narciso Paschoal, Raquel Eduardo Bickel, Daniela Peralva Lima, Jose Eduardo Goncalves, Maria Beatriz Abreu Gloria
Summary: The presence of mycotoxins in meat and meat products can result from contaminated feed carry-over, processing and storage contamination, and contaminated spices and ingredients. The occurrence of mycotoxins in dry fermented sausage is described worldwide, but little information is available for Brazilian products. This study investigated the occurrence of aflatoxins (B1, B2, G1, and G2) and ochratoxin A (OTA) in dry fermented sausage using a validated dilute and shoot method.
FOOD ANALYTICAL METHODS
(2023)
Article
Food Science & Technology
Evelyn Mayumi H. Konagano, Nelson R. Ferreira, Gilson C. A. Chagas-Junior, Maria Beatriz A. Gloria, Eloisa Helena De Aguiar Andrade, Lidiane D. Do Nascimento, Luiza Helena Da Silva Martins, Alessandra S. Lopes
Summary: This study investigated the influence of Cladosporium cladosporioides on cocoa fermentation. It was found that the use of C. cladosporioides starter culture led to changes in the physico-chemical characteristics, phenolic compounds, free bioactive amines, and volatile compounds in fermented and dried cocoa.
JOURNAL OF FOOD AND NUTRITION RESEARCH
(2022)
Article
Food Science & Technology
Samiria de Jesus Lopes Santos, Luiza Helena Meller da Silva, Antonio Manoel da Cruz Rodrigues
Summary: This study investigated the drying process of Dioscorea trifida and found it to have high dehydration rate and mass transfer coefficient. The mass transfer process became more efficient with increasing temperature. Additionally, the process was found to be endothermic rather than spontaneous.
FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Gilson Celso Albuquerque Chagas Junior, Nelson Rosa Ferreira, Maria Beatriz A. Gloria, Rubens Menezes Gobira, Felipe de Andrade Maia, Alessandra Santos Lopes
Summary: This study identified LAB in Amazonian cocoa prior to fermentation, and found five species that may contribute to enhancing the quality of chocolate.
FERMENTATION-BASEL
(2022)
Article
Chemistry, Analytical
Qian Wu, Li Tian, Xiangyu Shan, Huiling Li, Chao Li, Juan Lu
Summary: A novel ECL sensor was developed by modifying Fe3CuO4-Ru(bpy)3+ and GO-CdS@ZnS on the electrode for the detection of permethrin. The sensor exhibited high sensitivity and a wide detection range.
Article
Chemistry, Analytical
Tingting Gong, Lei Liao, Bingying Jiang, Ruo Yuan, Yun Xiang
Summary: In this study, a fluorescent biosensing system for high sensitivity detection of MT was established using a new Ag+-stabilized DNA triplex probe (Ag+-SDTP) combined with catalytic hairpin assembly (CHA) and CRISPR/Cas12a signal enhancements. The sensing method is able to selectively detect MT from other non-specific molecules and can achieve low level detection of MT in diluted human serums, demonstrating its potential for monitoring disease-specific MT biomarker at trace levels.
Article
Chemistry, Analytical
Estrella Sanz Rodriguez, Kyra Wood, Paul R. Haddad, Brett Paull
Summary: This study presents a simple, rapid, and reliable method for determining boron in aqueous solutions using suppressed ion chromatography coupled to electrospray ionisation-triple quadrupole mass spectrometry. The method achieved a low limit of detection and demonstrated excellent analytical performance.
Article
Chemistry, Analytical
Aline Cournut, Paul Moustiez, Yannick Coffinier, Christine Enjalbal, Claudia Bich
Summary: Alzheimer's disease is a major cause of senile dementia and is characterized by the aggregation of neurofibrillary tangles and amyloid plaques in the brain. The A beta 1-42 peptide is considered the primary cause of neurotoxicity due to its insolubility and subsequent aggregation. A beta 1-42 and A beta 1-40 have been identified as relevant biomarkers for AD diagnosis, and SurfaceAssisted Laser Desorption/Ionization Mass Spectrometry (SALDI-MS) was used to monitor the A beta 1-42/A beta 1-40 ratio without prior sample treatment.
Article
Chemistry, Analytical
Dotse Selali Chormey, Buse Tugba Zaman, Tulay Borahan Kustanto, Sezin Erarpat Bodur, Suleyman Bodur, Elif Ozturk Er, Sezgin Bakirdere
Summary: This review provides an overview of the harmful effects of endocrine disrupting chemicals (EDCs) on human health and introduces the methods for analyzing EDCs. Recent research has focused on using green chemicals, such as deep eutectic solvents (DESs), for microextraction to ensure environmental safety.
Article
Chemistry, Analytical
Yan Zhang, Renfeng Xu, Jingjing Wu, Zhenghong Zhang, Yuhuang Wang, Hongqin Yang, Sheng Zhang
Summary: This study visualized the pore-like structures in prostate cancer cell membranes after treatment with the anticancer drug paclitaxel, demonstrating its mechanism of action related to oxidative damage.
Article
Chemistry, Analytical
Chihong Ma, Qin Zhou, Jinjin Shi, Hua Gao, Di Huang, Huimin Xue, Han Wang, Zhenzhong Zhang, Sen Yang, Junli Zhang, Kaixiang Zhang
Summary: We developed a CRISPR-empowered electrochemical biosensor, named PER-E-CRISPR, for amplification-free and sensitive detection of miR-21. The biosensor combines the advantages of CRISPR/Cas13a and primer exchange reaction (PER) for target amplification. Under optimized conditions, the PER-E-CRISPR assay exhibits a linear range from 10-13 to 10-7 M and a limit of detection of 30.2 fM. The biosensor shows excellent performance in actual plasma, indicating promising prospects for miRNA detection in molecular diagnosis.
Article
Chemistry, Analytical
Gang Liang, Jie Zhao, Yufei Gao, Tao Xie, Jianhui Zhen, Ligang Pan, Wenwen Gong
Summary: Molecular docking (MD) analysis is widely used for studying the interaction between aptamers and small molecules. This study established steady-state aptamers of tertiary structures (SATS) using specific aptamers of tetracycline antibiotics as docking models. Molecular docking results revealed multiple binding sites in the SATS of aptamers, with significant variations in binding free energy (BFE) and docking score (DS). The study also proposed a method for MD analysis based on SATS, providing insights into binding mode and predicting binding sites.
Article
Chemistry, Analytical
Yixuan Xu, Changchang Teng, Huiping Dang, Dalong Yin, Lifeng Yan
Summary: Stable organic radical molecules with high NIR fluorescence quantum efficiency have been synthesized and encapsulated into nanoparticles, which show great potential for both photodynamic therapy (PDT) and photothermal therapy (PTT) of tumors.
Article
Chemistry, Analytical
Yu Su, Dandan Yang, Yanjie Wang, Jie Ding, Lan Ding, Daqian Song
Summary: In this study, a highly selective molecularly imprinted polymer Cu-MIPs@CS was designed and synthesized for bisphenol A (BPA). Cu-MIPs@CS showed significantly enhanced imprinting factor and selective factor towards BPA compared to MIPs@CS. It also exhibited superior discrimination ability between BPA and its structural analogue. Additionally, Cu-MIPs@CS were successfully applied as a solid phase extraction adsorbent for the determination of BPA in drinking water samples.
Article
Chemistry, Analytical
Yi Qiao, Qiongdan Zhang, Yukun He, Tianguang Cheng, Jing Tu
Summary: Single cell heterogeneity plays an important role in many biological phenomena, and distinguishing cells with specific mutations is beneficial for clinical diagnosis and drug screening. This study introduces a gel plate platform that can distinguish cell types based on their phenotypes, with low equipment requirement. The gel platform has potential in point-of-care circumstances and single-cell stimulation responses that prioritize efficiency and simplicity.
Article
Chemistry, Analytical
Chengke Wang, Shuyang Sun, Ping Wang, Huawei Zhao, Wenling Li
Summary: The food safety of aquatic products is a global concern. Nanotechnology-based analyses have advantages in the detection of bacteria, metal ions, and small molecule contaminants. This review summarizes the recent advances in biosensing strategies for aquatic products and highlights the application of nanomaterials, lateral flow-based biosensors, surface-enhanced Raman scattering, microfluidic chips, and molecular imprinting technologies.
Article
Chemistry, Analytical
Liping Zhu, Zeng Tang, Xuemei Zhang, Li Zhu, Tian Meng, Linying Yu, Ting Xiao, Shasha Lu, Xiaoli Xiong, Xiurong Yang
Summary: A sensitive off-on electrochemiluminescence DNA sensor was developed using Exo III-assisted cascade amplification system. The sensor achieved high selectivity and ultra-high sensitivity in detecting target DNA by utilizing ECL and SPR effects. This study provides a new perspective for designing highly sensitive and programmable ECL biosensors.
Article
Chemistry, Analytical
Tong Li, Mengxu Sun, Suping Xia, Ting Huang, Rong-Tian Li, Chunrong Li, Zong Dai, Jin-Xiang Chen, Jun Chen, Nuan Jia
Summary: Here, we developed a binary system based on DNA tetrahedron and fluorogenic RNA aptamers for highly specific and label-free mRNA imaging in living cells. This system achieved high specificity label-free detection of nucleic acids with a detection limit of 1.34 nM. The method has potential applications in early disease diagnosis and new drug development.
Article
Chemistry, Analytical
Yao Zhao, Shu Zhang, Wang Yao, Yuxuan Zhu, Jing Qian, Juan Yang, Nianjun Yang
Summary: A new hierarchical nanostructured composite with high conductivity and catalytic activity is synthesized. The sensor exhibits significant catalytic effect and high sensitivity for monitoring the environmental endocrine disruptor bisphenol A (BPA).
