期刊
SYSTEMATIC AND APPLIED MICROBIOLOGY
卷 40, 期 3, 页码 123-134出版社
ELSEVIER GMBH
DOI: 10.1016/j.syapm.2017.01.003
关键词
MALDI-TOF MS; Acetic acid bacteria; Taxonomy; Whole genome sequencing; ANI
资金
- Federal Public Planning Service-Science Policy, Belgium
- Research Foundation-Flanders (FWO-Vlaanderen)
- Research Council of Ghent University
- Vrije Universiteit Brussel
- Hercules Foundation
- Chinese Scholarship Council
- Ghent University
The classification and identification of acetic acid bacteria (AAB) underwent much change during the last two decades. However, several closely related AAB species are difficult to differentiate and rapid and correct species level identification remains challenging. In the present study, 281 well-characterized AAB strains representing AAB species of the family Acetobacteraceae were used to construct a matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) identification database. The database was evaluated using 270 poorly characterized AAB strains. 16S rRNA and/or multilocus gene sequence analysis and whole-genome sequencing for the calculation of average nucleotide identity values were performed on a selection of strains to verify the accuracy of the obtained results. Virtually all poorly characterized AAB strains were accurately identified to the species level. Most of the remaining strains were considered to represent novel AAB species or belonged to species represented by too few strains in the identification database. The data also revealed that Gluconobacter nephelii and Gluconobacter uchimurae are later heterotypic synonyms of Gluconobacter japonicus and Gluconobacter oxydans, respectively. (C) 2017 Elsevier GmbH. All rights reserved.
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