Article
Biochemistry & Molecular Biology
Aleksandra Walewska, Paulina Kosikowska-Adamus, Marta Tomczykowska, Bartosz Jaroszewski, Adam Prahl, Grzegorz Bulaj
Summary: Human beta-defensin 3 (HBD-3) is an antimicrobial and immunomodulatory peptide with multiple biological functions. This study optimized the synthetic method of HBD-3, improving the synthesis efficiency and enabling structural and functional studies. Additionally, an alternative synthesis method with improved oxidative folding was proposed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Applied
Giuseppina Sabatino, Annunziata D'Ercole, Lorenzo Pacini, Matteo Zini, Arianna Ribecai, Alfredo Paio, Paolo Rovero, Anna Maria Papini
Summary: The study utilized microwave-assisted solid-phase peptide synthesis to prepare Eptifibatide and explored various solution and solid-phase disulfide formation methods. The research found that the solid-phase strategy optimized the preparation process, resulting in the development of an automated production procedure.
ORGANIC PROCESS RESEARCH & DEVELOPMENT
(2021)
Article
Chemistry, Applied
Dmitry Avdeev, Michael V. Ovchinnikov, Michael G. Medvedev, Alexander S. Molokoedov, Maria V. Sidorova
Summary: An industry-ready approach for the synthesis of terlipressin is developed, which includes the assembly of the peptide chain and closure of the disulfide bond on a polymeric carrier with specific precautions for protecting the N-terminal a-amino group. Factors influencing the purity of crude disulfide and the optimization of the solid-phase cyclization process were studied. The approach has high fidelity and reproducibility, making it suitable for large-scale peptide synthesis (up to 15 g of the pure product demonstrated). Molecular modeling revealed the significant influence of the state of the N-terminal amino group (free or protected) on the ability of two cysteine side chains to interact, providing insights for rational choice of protecting groups in peptide synthesis.
ORGANIC PROCESS RESEARCH & DEVELOPMENT
(2023)
Article
Chemistry, Analytical
Sheng Wang, Peize Wu, Ming Li, Ting Huang, Naijie Shi, Liuxing Feng, Hongmei Li
Summary: Oxytocin is important for medical purposes and there is a need for standardization in its assays. A method for purity assignment of synthetic oxytocin with a disulfide linkage was developed, and impurities were identified and quantified using liquid chromatography-high-resolution tandem mass spectrometry. The study also assessed non-peptide related impurities using various analytical techniques, with a resulting assigned value traceable to SI with a small measurement uncertainty.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2022)
Article
Chemistry, Organic
Jan Pawlas, Jon H. Rasmussen
Summary: This study reports a catalytic approach to solid-phase peptide synthesis, which utilizes readily available reagents and an inexpensive green solvent. The method demonstrates high stereoselectivity in couplings and achieves high purity in peptide synthesis.
Article
Chemistry, Multidisciplinary
Jan Pawlas, Jon H. Rasmussen
Summary: This study demonstrates the feasibility of using Fmoc/t-Bu solid-phase peptide synthesis under aqueous conditions, utilizing a resin and solvent combination to improve resin swelling and solubility of starting materials. By developing a waste recycling method, the reuse of waste was achieved, reducing the amount of starting materials used and replacing hazardous solvents with an aqueous medium.
Article
Chemistry, Organic
Ryan Moreira, Jacob Wolfe, Scott D. Taylor
Summary: Researchers have developed a new total synthesis method for daptomycin with a high overall yield of 22% by investigating reactions in both solid-phase and solution conditions. The simplicity and efficiency of this new method will aid in synthesizing analogs of daptomycin. Additionally, elucidating the mechanism of this side reaction simplifies the process of preparing other kynurenine-containing natural products via Fmoc SPPS.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2021)
Article
Chemistry, Physical
Fengyi Cao, Gangqing Ma, Lin Mei, Genxing Zhu, Meng Song, Qi Qin, Mingli Jiao
Summary: In this study, a cysteine-containing PAF26 peptide derivative was designed to develop a covalently crosslinked antimicrobial hydrogel, which showed strong crosslinked networks and excellent antimicrobial abilities against Candida albicans, Staphylococcus aureus, and Escherichia coli.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2021)
Article
Biochemistry & Molecular Biology
Zi-Fu Ni, Na Li, Pei Xu, Ze-Wang Guo, Min-Hua Zong, Wen-Yong Lou
Summary: This study constructed disulfide bond and backbone cyclization variants of EcMAL enzyme to improve its catalytic activity and thermostability. The combination strategy showed synergistic promotion effect on activity and stability, providing a new method for adjusting enzyme thermostability through modification away from the active center.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Polymer Science
Xiaoxing Zhang, Hui Lin, Yiran Hu, Dalin Ding, Fujin Cai, Yunjian Wu
Summary: This study successfully synthesized high-performance epoxy insulating materials with varying disulfide bond contents, which enhance the mechanical properties of the epoxy resin while fulfilling the prerequisites for electrical applications. The self-healing test showed an optimal repair efficiency of 81.23%.
POLYMER DEGRADATION AND STABILITY
(2023)
Article
Chemistry, Organic
Jie-Sheng Tian, Yu He, Zhi-Yuan Gao, Xu Liu, Shuang-Feng Dong, Peng Wu, Teck-Peng Loh
Summary: A metal-free, water-tolerant, one-pot process for ortho-acylation of phenols has been developed, promoted by the iodine source/hydrogen peroxide system. This transformation involves ether formation, iodocyclization, C-C bond cleavage, and oxidative hydrolysis in a one-step manner, as supported by control experiments.
Article
Chemistry, Multidisciplinary
Peter H. G. Egelund, Sandip Jadhav, Vincent Martin, Henrik Johansson Castro, Franziska Richner, Sebastian Thordal Le Quement, Frank Dettner, Carolin Lechner, Ralph Schoenleber, Daniel Sejer Pedersen
Summary: This study explores the use of pyrrolidine as a base to expand the available solvent polarity space for green solid-phase peptide synthesis, investigating the effects of different solvent mixtures on Fmoc removal and coupling reactions, and comparing the synthesis results of different peptide targets.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2021)
Article
Food Science & Technology
Weijia Zhu, Liqin Qin, Youqiang Xu, Hongyun Lu, Qiuhua Wu, Weiwei Li, Chengnan Zhang, Xiuting Li
Summary: Three strategies were investigated to improve the thermostability of xylanase, including reducing surface entropy, constructing intramolecular disulfide bonds, and molecular cyclization. Molecular simulations showed that all mutants exhibited improved thermostability and catalytic efficiency compared to the wild-type enzyme.
Article
Chemistry, Multidisciplinary
Huanyu Tao, Qilong Wu, Xuejun Zhao, Peicong Lin, Sheng-You Huang
Summary: MODPEP2.0 is an efficient tool for sampling cyclic peptide structures, which can quickly generate conformations of cyclic peptides with considerable accuracy and computational efficiency compared to other sampling algorithms.
JOURNAL OF CHEMINFORMATICS
(2022)
Article
Chemistry, Multidisciplinary
Dong-in Kim, Seong-jae Han, Yong-beom Lim
Summary: This study utilizes cyclic proteins and peptides to determine the maximum possible bending in the alpha-helix and finds that the radius of curvature is approximately 10 times smaller than those of typical alpha-helices found in natural proteins. This finding opens up possibilities for designing de novo peptides and proteins with unique structures and peculiar functions.
CHEMICAL COMMUNICATIONS
(2022)
Article
Chemistry, Multidisciplinary
Xin Zhang, Pengcheng Sun, Lingzi Huangshan, Bi-Huang Hu, Phillip B. Messersmith
CHEMICAL COMMUNICATIONS
(2015)
Article
Engineering, Biomedical
George W. Dombi, Kaushalkumar Purohit, Lenore M. Martin, Sze C. Yang
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE
(2015)
Article
Biochemistry & Molecular Biology
Giovanni J. Pagano, Roberta S. King, Lenore M. Martin, Linda A. Hufnagel
PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS
(2015)
Article
Engineering, Biomedical
Jing Su, Bi-Huang Hu, William L. Lowe, Dixon B. Kaufman, Phillip B. Messersmith
Article
Biophysics
Ming Gong, Yan-Bing Wang, Ming Li, Bi-Huang Hu, Yong-Kuan Gong
COLLOIDS AND SURFACES B-BIOINTERFACES
(2011)
Article
Biotechnology & Applied Microbiology
Keerthi P. Venkataramanan, Yogi Kurniawan, Judy J. Boatman, Cassandra H. Haynes, Katherine A. Taconi, Lenore Martin, Geoffrey D. Bothun, Carmen Scholz
JOURNAL OF BIOTECHNOLOGY
(2014)
Article
Chemistry, Organic
Zhongqiang Liu, Bi-Huang Hu, Phillip B. Messersmith
TETRAHEDRON LETTERS
(2010)
Article
Chemistry, Physical
Faramarz Joodaki, Lenore M. Martin, Michael L. Greenfield
Summary: The study investigated the hinge formation in the designed antimicrobial peptide LM7-2 through simulations of its dynamics and vibrations in solution. Results showed that the formation of a helix-hinge-helix conformation in LM7-2 led to altered frequencies in amide bands II and III, indicating a structural change affecting its inherent vibrations. The research also suggested a mechanism for hinge formation involving hydrogen bonding with water molecules replacing intramolecular hydrogen bonds as the peptide backbone folds.
JOURNAL OF PHYSICAL CHEMISTRY B
(2021)
Article
Chemistry, Multidisciplinary
Faramarz Joodaki, Lenore M. Martin, Michael L. Greenfield
Summary: Studies have shown that the cell membrane plays a crucial role in the antibiotic resistance of Staphylococcus aureus. Researchers developed a complex computational model to simulate the structure and dynamics of the membrane. The results revealed the thickness of the membrane and the ordering of molecules, with lower ordering observed in carbon atoms of branched lipids.
Article
Chemistry, Physical
Michael L. Greenfield, Lenore M. Martin, Faramarz Joodaki
Summary: Lipid bilayers undergo phase transitions and exhibit different structures depending on temperature and lipid surface concentration. The area per lipid head group and the radial distribution function are used to characterize the bilayer phase. A novel method based on Voronoi tessellation is introduced to determine the area distribution of individual lipids in a multicomponent system. The results show that lipids and water molecules occupy similar areas in the interfacial region, and lipid tails contribute significantly to the membrane side of the bilayer-water interface.
JOURNAL OF PHYSICAL CHEMISTRY B
(2022)
Article
Chemistry, Physical
Faramarz Joodaki, Lenore M. Martin, Michael L. Greenfield
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2019)
Meeting Abstract
Cell Biology
G. Pagano, R. King, L. M. Martin, J. Schumacher, L. A. Hufnagel
MOLECULAR BIOLOGY OF THE CELL
(2012)
Meeting Abstract
Biochemistry & Molecular Biology
Roxanne LaCroix, Krishna Tamminedi, David E. Ryder, Jay F. Sperry, Lenore M. Martin
Meeting Abstract
Biochemistry & Molecular Biology
K. Tamminedi, B. Chaudhary, J. Sperry, R. LaCroix, L. Martin