Review
Biotechnology & Applied Microbiology
Martina Sandona, Lorena Di Pietro, Federica Esposito, Alessia Ventura, Antonietta Rosa Silini, Ornella Parolini, Valentina Saccone
Summary: Mesenchymal stromal cells (MSCs) are multipotent cells found in various tissues that have anti-fibrotic and immunoregulatory properties. Their secretome, including extracellular vesicles (EVs), can mimic the therapeutic effects of MSCs and offer advantages such as easier storage and safer administration for muscle regeneration in different diseases.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Simone Vumbaca, Giulio Giuliani, Valeria Fiorentini, Flavia Tortolici, Andrea Cerquone Perpetuini, Federica Riccio, Simona Sennato, Cesare Gargioli, Claudia Fuoco, Luisa Castagnoli, Gianni Cesareni
Summary: The study investigated changes in skeletal muscle secretome during regeneration process to identify potential regulatory circuits and cell-cell interactions. The analysis included profiling gene expression of various cell types and cytokines, and testing the modulation of adipogenesis and proliferation by selected cytokines. Furthermore, the study explored the interaction mediated by extracellular vesicles and their influences on cell fate during muscle regeneration.
Review
Biochemistry & Molecular Biology
Karolina Archacka, Maria A. Ciemerych, Anita Florkowska, Karolina Romanczuk
Summary: miRNAs and lncRNAs play crucial roles in gene expression regulation, especially in myogenic differentiation. Manipulating the levels of specific miRNAs and lncRNAs can improve myogenic differentiation of stem and progenitor cells, while physical activity can affect the expression of miRNAs and lncRNAs, ultimately impacting muscle health.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Pharmacology & Pharmacy
Vera Vorstandlechner, Dragan Copic, Katharina Klas, Martin Direder, Bahar Golabi, Christine Radtke, Hendrik J. Ankersmit, Michael Mildner
Summary: This study demonstrates the anti-fibrotic effect of PBMCsec on cutaneous scars in both mouse and human experimental settings. PBMCsec regulates the expression of fibrosis-related genes, attenuates myofibroblast differentiation, and inhibits the breakdown of elastic fibers. These findings suggest that PBMCsec could be a novel therapeutic option for treating skin scarring.
Review
Cell Biology
Han Su, Aubrey C. Cantrell, Heng Zeng, Shai-Hong Zhu, Jian-Xiong Chen
Summary: Pericytes, as mural cells covering microvascular capillaries, play essential roles in vascular remodeling and maintenance, interact with other cells via secretome mechanisms, and have significant impacts on vasculature formation, stabilization, and regeneration. They also function as mesenchymal cells or progenitor cells in cardiovascular regeneration, contributing to vascular remodeling in various ways.
Review
Polymer Science
Ranya Ibrahim, Hillary Mndlovu, Pradeep Kumar, Samson A. Adeyemi, Yahya E. Choonara
Summary: There is significant interest in using stem cells for cutaneous wound management, but concerns over safety, efficacy, and cost limit their clinical application. Secretome, a collection of paracrine factors and cytokines stored in mesenchymal stem cells, shows promise as a cell-free therapy for treating cutaneous wounds and promoting tissue regeneration.
Article
Biochemistry & Molecular Biology
Wei Wei, Nicholas M. Riley, Andrew C. Yang, Joon T. Kim, Stephanie M. Terrell, Veronica L. Li, Marta Garcia-Contreras, Carolyn R. Bertozzi, Jonathan Z. Long
Summary: This study introduced a proximity biotinylation strategy to label, detect, and enrich secreted polypeptides in a cell type-selective manner in mice, resulting in a proteomic atlas of secretomes from various cell types. The research confirmed known cell type-protein pairs, identified new secreted polypeptides distinguishing between cell types, and uncovered a dynamic nutrient-dependent reprogramming of hepatocyte secretome. The strategy enables dynamic and cell type-specific dissection of the plasma proteome and intercellular signaling mediators.
NATURE CHEMICAL BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Batoul Chouaib, Mandana Haack-Sorensen, Franck Chaubron, Frederic Cuisinier, Pierre-Yves Collart-Dutilleul
Summary: Mesenchymal stem cell secretome or conditioned medium (MSC-CM) is a crucial starting point for various derived products and can be used for regenerative medicine. However, issues such as manufacturing protocols and quality control must be addressed before their clinical application. This review highlights the importance of standardized procedures in conditioned medium production and the influence of various factors on the quality of the secretome and its derivatives.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Cell Biology
Gregory J. Konar, Claire Ferguson, Zachary Flickinger, Matthew R. Kent, James G. Patton
Summary: The study has identified the genetic pathways and components required for retina regeneration, as well as the key regulatory events controlling the process. Muller glia are reprogrammed as adult retinal stem cells, while miRNAs are recognized as key gene regulatory molecules in controlling retina regeneration.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Michael A. Lopez, Ying Si, Xianzhen Hu, Valentyna Williams, Fuad Qushair, Jackson Carlyle, Lyndsy Alesce, Michael Conklin, Shawn Gilbert, Marcas M. Bamman, Matthew S. Alexander, Peter H. King
Summary: Duchenne muscular dystrophy (DMD) is characterized by muscle instability, wasting, and fibrosis. This study found an aberrant upregulation of a signaling pathway and a decrease in specific microRNAs in DMD patient muscle cells. Inhibition of this pathway promoted muscle cell differentiation and suppressed inflammation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Yaling Yang, Jian Wu, Wujun Liu, Yumin Zhao, Hong Chen
Summary: This paper reviews the non-coding RNAs related to the growth and development of animal skeletal muscles, aiming to provide reference for the study of ncRNAs' role in the development of animal skeletal muscles and to offer new ideas for improving animal production performance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Rui D. Alvites, Mariana V. Branquinho, Ana C. Sousa, Bruna Lopes, Patricia Sousa, Justina Prada, Isabel Pires, Giulia Ronchi, Stefania Raimondo, Ana L. Luis, Stefano Geuna, Artur Severo P. Varejao, Ana Colette Mauricio
Summary: Cell secretome is of great interest in Regenerative Medicine. This study analyzed the secretome collected from Olfactory Mucosa Mesenchymal Stem Cells and Olfactory Ensheathing Cells and applied it to promote peripheral nerve regeneration. The results showed that this therapeutic combination had beneficial effects on nerve and muscle regeneration, but further research is needed to address irregularities in the outcomes.
Article
Biochemistry & Molecular Biology
Roberta Squecco, Alessia Tani, Flaminia Chellini, Rachele Garella, Eglantina Idrizaj, Irene Rosa, Sandra Zecchi-Orlandini, Mirko Manetti, Chiara Sassoli
Summary: This study demonstrates that bone marrow-mesenchymal stem/stromal cells (MSCs) secretome has positive effects on skeletal muscle repair, attenuating tissue damage and protecting muscle fibers from apoptosis. Additionally, MSC secretome affects key cells involved in muscle regeneration, contributing to the replenishment of muscle satellite cells and functional modifications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Cell Biology
Yingying Chen, Qinghong Xia, Yue Zeng, Yun Zhang, Meixia Zhang
Summary: Muller glia play critical roles in regulating retinal inflammation through metabolic regulation and miRNA-mediated mechanisms. Novel therapies targeting Muller glia are being explored for the treatment of inflammatory retinal diseases.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Pharmacology & Pharmacy
Yan Yan, Ming Li, Jie Lin, Yanan Ji, Kexin Wang, Dajun Yan, Yuntian Shen, Wei Wang, Zhongwei Huang, Haiyan Jiang, Hualin Sun, Lei Qi
Summary: This review summarizes the role of AMPK in regulating mitochondrial function and its impact on skeletal muscle metabolism and health.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Biochemical Research Methods
Laween Meran, Lucinda Tullie, Simon Eaton, Paolo De Coppi, Vivian S. W. Li
Summary: The authors present a protocol for bioengineering human intestinal mucosal grafts using patient-derived materials. This technology has significant potential for treating intestinal failure and studying human gastrointestinal diseases.
Article
Genetics & Heredity
Yada Kunpalin, Simen Vergote, Luc Joyeux, Onur Telli, Anna L. David, Michael Belfort, Paolo De Coppi, Jan Deprest
Summary: A study on commercially available patches used in fetal rabbit models for spina bifida repair found that covering the spinal cord with Duragen or Durepair and closing the skin preserves motor neuron density and reduces the inflammatory response.
PRENATAL DIAGNOSIS
(2023)
Article
Pediatrics
Cunera M. C. de Beaufort, Dalia Aminoff, Ivo de Blaauw, Celia Cretolle, Jens Dingemann, Natalie Durkin, Wout F. J. Feitz, JoAnne Fruithof, Caterina Grano, Carmen Mesas Burgos, Nicole Schwarzer, Graham Slater, Tutku Soyer, Cristiano Violani, Rene Wijnen, Paolo de Coppi, Ramon R. Gorter
Summary: The aim of this international study was to assess the current status of transition of care (TOC) and adult care (AC) programs for patients with anorectal malformations (ARM) and Hirschsprung disease (HD). The results showed that less than 50% of the participating centers had installed these programs, and there was considerable heterogeneity in the content and implementation of the programs. Sharing best practice examples and considering local and national healthcare programs may improve the continuity of care in the future.
JOURNAL OF PEDIATRIC SURGERY
(2023)
Article
Acoustics
S. Vergote, F. R. De Bie, J. M. N. Duffy, J. Bosteels, A. Benachi, B. Power, F. Meijer, H. L. Hedrick, C. J. Fernandes, I. K. M. Reiss, P. De Coppi, K. P. Lally, J. A. Deprest
Summary: A core outcome set for evaluating perinatal interventions in congenital diaphragmatic hernia was developed with the input of stakeholders, standardizing the selection and definition of outcomes and facilitating future research to guide clinical practice.
ULTRASOUND IN OBSTETRICS & GYNECOLOGY
(2023)
Article
Multidisciplinary Sciences
Anna Urciuolo, Giovanni Giuseppe Giobbe, Yixiao Dong, Federica Michielin, Luca Brandolino, Michael Magnussen, Onelia Gagliano, Giulia Selmin, Valentina Scattolini, Paolo Raffa, Paola Caccin, Soichi Shibuya, Dominic Scaglioni, Xuechun Wang, Ju Qu, Marko Nikolic, Marco Montagner, Gabriel L. Galea, Hans Clevers, Monica Giomo, Paolo De Coppi, Nicola Elvassore
Summary: This study develops a hydrogel-in-hydrogel bioprinting approach to dynamically control the growth landscape of three-dimensional cell cultures. The technique enables modulation of hydrogel characteristics to guide neural axon directionality, control cell migration in cancer organoids, and promote cell polarity in liver and small intestinal organoids, as well as control lung tip bifurcation.
NATURE COMMUNICATIONS
(2023)
Article
Nutrition & Dietetics
Robertina Giacconi, Francesco Piacenza, Valentina Aversano, Michele Zampieri, Alexander Buerkle, Maria Moreno Villanueva, Martijn E. T. Dolle, Eugene Jansen, Tilman Grune, Efstathios S. Gonos, Claudio Franceschi, Miriam Capri, Birgit Weinberger, Ewa Sikora, Olivier Toussaint, Florence Debacq-Chainiaux, Wolfgang Stuetz, Pieternella Eline Slagboom, Juergen Bernhardt, Maria Luisa Fernandez-Sanchez, Mauro Provinciali, Marco Malavolta
Summary: Plasma selenium levels are influenced by age, sex, nutrition, and inflammation. Inadequate selenium intake can accelerate aging and increase the risk of age-related diseases.
Article
Cell Biology
Hannah Ee Juen Yong, Katarzyna Maksym, Muhammad Ashraf Bin Yusoff, Esteban Salazar-Petres, Tatiana Nazarenko, Alexey Zaikin, Anna L. David, Sara L. Hillman, Amanda N. Sferruzzi-Perri
Summary: Fetal growth restriction (FGR) is a major cause of perinatal morbidity and mortality. This study explores the relationship between placental structure and function in healthy and FGR pregnancies through predictive modeling. The findings provide new insights into the etiology of FGR.
Article
Pediatrics
Amulya Saxena, Annika Mutanen, Ramon Gorter, Andrea Conforti, Pietro Bagolan, Paolo De Coppi, Tutku Soyer
Summary: This study reviewed the literature on the management of neonatal ovarian simple cysts and formulated a consensus statement based on current evidence and peer practice. Although there is limited data on the potential benefits and complications of prenatal ultrasound-guided aspiration, neonates after such procedures should be followed for 6 months. Neonates with simple ovarian cysts larger than 4 cm should undergo surgical interventions within the first two weeks of life, with laparoscopic cyst aspiration and fenestration using bipolar instruments as the preferred approach. Ultrasound follow-up should be done at 3 months after surgical intervention and every 3 to 4 months with the conservative approach until 1 year.
EUROPEAN JOURNAL OF PEDIATRIC SURGERY
(2023)
Article
Biology
Panagiota Kolitsida, Vladimir Nolic, Jianwen Zhou, Michael Stumpe, Natalie M. Niemi, Jorn Dengjel, Hagai Abeliovich
Summary: The mitophagic degradation of mitochondrial matrix proteins in Saccharomyces cerevisiae is selectively regulated by the matrix kinases Pkp1 and Pkp2, which are in turn regulated by the phosphatase Aup1/Ptc6. These proteins also regulate the phosphorylation status and catalytic activity of the pyruvate dehydrogenase complex, which is critical for mitochondrial metabolism. The pyruvate dehydrogenase complex has been found to have a novel function in regulating mitophagic selectivity independent of its enzymatic activity.
LIFE SCIENCE ALLIANCE
(2023)
Article
Geriatrics & Gerontology
Bastian Kochlik, Catrin Herpich, Maria Moreno-Villanueva, Susanne Klaus, Ursula Mueller-Werdan, Birgit Weinberger, Simone Fiegl, Olivier Toussaint, Florence Debacq-Chainiaux, Christiane Schoen, Juergen Bernhard, Nicolle Breusing, Efstathios S. Gonos, Claudio Franceschi, Miriam Capri, Ewa Sikora, Antti Hervonen, Mikko Hurme, P. Eline Slagboom, Martijn E. T. Dolle, Eugene Jansen, Tilman Grune, Alexander Buerkle, Kristina Norman
Summary: Growth differentiation factor-15 (GDF15) is linked to cognitive frailty and depression, with high levels of GDF15 associated with both conditions. This study analyzed data from the MARK-AGE study and found that high GDF15 concentrations were significantly associated with combined cognitive-frailty-and-depression status in both older and younger adults. These associations remained significant even after adjusting for age, BMI, sex, comorbidities, and hsCRP.
Review
Biochemistry & Molecular Biology
Joelle Giroud, Ines Bouriez, Hugo Paulus, Albin Pourtier, Florence Debacq-Chainiaux, Olivier Pluquet
Summary: Cellular senescence is a complex cell state that can occur during physiological aging or after exposure to stress signals, regardless of age. Senescent cells interact with their microenvironment by producing a heterogenous and plastic secretome referred to as the senescence-associated secretory phenotype (SASP). Understanding the cross-talk between SASP and the microenvironment is challenging due to the complexity of signal exchanges.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Biswajoy Bagchi, Priyankan Datta, Carmen Salvadores Fernandez, Priya Gupta, Shireen Jaufuraully, Anna L. L. David, Dimitrios Siassakos, Adrien Desjardins, Manish K. K. Tiwari
Summary: This study presents a flexible and cost-effective triboelectric nanogenerator (TENG) using oxidation-resistant pure copper nanowires as electrodes. The device demonstrated stable performance with an impressive open circuit voltage of 200 V and power density of 10.67 W m(-2). It is robust, flexible, transparent, and non-cytotoxic, making it promising for various energy harvesting and advanced healthcare applications.
MATERIALS HORIZONS
(2023)
Review
Genetics & Heredity
Rachel L. Sagar, Lilian Walther-Jallow, Cecilia Gotherstrom, Magnus Westgren, Anna L. David
Summary: The maternal and fetal safety of In utero stem cell transplantation (IUSCT) was investigated. A systematic review found that the acute fetal complication rate of IUSCT was 4.5% per procedure. Maternal adverse events and pregnancy outcomes have been poorly reported.
PRENATAL DIAGNOSIS
(2023)
Article
Medicine, Research & Experimental
Rebecca Spencer, Kasia Maksym, Kurt Hecher, Karel Marsal, Francesc Figueras, Gareth Ambler, Harry Whitwell, Nuno Rocha Nene, Neil J. Sebire, Stefan R. Hansson, Anke Diemert, Jana Brodszki, Eduard Gratacos, Yuval Ginsberg, Tal Weissbach, Donald M. Peebles, Ian Zachary, Neil Marlow, Angela Huertas-Ceballos, Anna L. David
Summary: This study investigates the use of maternal serum protein and ultrasound measurements to predict outcomes in pregnancies affected by severe, early-onset fetal growth restriction (FGR). The results demonstrate that ultrasound measurements and maternal serum PlGF concentration can predict important pregnancy outcomes for patients and clinicians.
JOURNAL OF CLINICAL INVESTIGATION
(2023)
Correction
Engineering, Biomedical
Oluwatosin Alabi, Sophia Bano, Francisco Vasconcelos, Anna L. David, Jan Deprest, Danail Stoyanov
INTERNATIONAL JOURNAL OF COMPUTER ASSISTED RADIOLOGY AND SURGERY
(2023)