Review
Microbiology
Valerio M. Sora, Gabriele Meroni, Piera A. Martino, Alessio Soggiu, Luigi Bonizzi, Alfonso Zecconi
Summary: The One Health approach highlights the importance of antimicrobial resistance in both public health and food animal production, with specific focus on the ExPEC strains of E. coli which may transfer resistance genes to other pathogenic bacteria, posing a serious threat to public health globally.
Article
Infectious Diseases
Isabella F. S. Figueiredo, Lorena G. Araujo, Raissa G. Assuncao, Itaynara L. Dutra, Johnny R. Nascimento, Fabricia S. Rego, Carolina S. Rolim, Leylane S. R. Alves, Mariana A. Frazao, Samilly F. Cadete, Luis Claudio N. da Silva, Joicy C. de Sa, Eduardo M. de Sousa, Waldir P. Elias, Flavia R. F. Nascimento, Afonso G. Abreu
Summary: The study showed that cinnamaldehyde has potential in treating sepsis by reducing inflammation, increasing cell production in bone marrow and spleen, and improving survival rates in animals.
Article
Infectious Diseases
Amel Mhaya, Rahma Trabelsi, Sabine Aillerie, Fatima M'Zali, Dominique Begu, Slim Tounsi, Radhouane Gdoura, Corinne Arpin
Summary: Multiple ESBL-producing strains were identified in Tunisian patients, including two emerging virulent clones, ST131-C2 and ST131-C1-M27, which were found to be prevalent in both community and hospital settings.
Article
Immunology
Ying Yu, Bo Hu, Huanhuan Fan, Hailing Zhang, Shizhen Lian, Hongye Li, Shuangshuang Li, Xijun Yan, Shaohui Wang, Xue Bai
Summary: The study identified molecular epidemiology and biological characteristics of Escherichia coli associated with hemorrhagic pneumonia (HP) in mink from five Chinese provinces. Analysis included serotyping, antimicrobial susceptibility, detection of virulence genes, phylogenetic grouping, whole-genome sequencing, drug resistant gene, multilocus sequence typing (MLST), and biofilm formation assays.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Microbiology
Victoria Ballen, Yaiza Gabasa, Carlos Ratia, Melany Sanchez, Sara Soto
Summary: This study characterized 376 extraintestinal pathogenic E. coli strains collected from hospitals in Catalonia, Spain, between 2016 and 2017. The strains showed high resistance to several antibiotics and varied biofilm formation ability. Various virulence and antimicrobial resistance genes were detected, including the colibactin-encoding genes associated with colorectal cancer. The study also investigated the relationship between colibactin and biofilm formation.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Urology & Nephrology
Steven Rosenberg, Marc Bonten, Wouter Haazen, Bart Spiessens, Darren Abbanat, Oscar Go, Florian Wagenlehner, Neal Shore, Yosuke Hagiwara, Patricia Ibarra de Palacios, Jeroen Geurtsen, Peter Hermans, Jan Poolman
Summary: This study evaluated the incidence of microbiologically confirmed invasive ExPEC disease in patients undergoing TRUS-PNB, finding the highest incidence in the U.S. The prevalence of 10 selected O-serotypes was 52.0%, and E. coli isolates showed highest resistance rates to levofloxacin and ciprofloxacin at 76%.
JOURNAL OF UROLOGY
(2021)
Article
Immunology
Eveline Weerdenburg, Todd Davies, Brian Morrow, Aldert L. Zomer, Peter Hermans, Oscar Go, Bart Spiessens, Thijs van den Hoven, Gunter van Geet, Moussa Aitabi, Chitrita DebRoy, Edward G. Dudley, Marc Bonten, Jan Poolman, Jeroen Geurtsen
Summary: Extraintestinal pathogenic Escherichia coli (ExPEC) is a major cause of bacteremia worldwide, and the dominant O serotype among ExPEC bacteremia isolates is O25B, particularly among multidrug-resistant isolates.
CLINICAL INFECTIOUS DISEASES
(2023)
Article
Food Science & Technology
Lucas Harrison, Gregory H. Tyson, Errol Strain, Rebecca L. Lindsey, Nancy Strockbine, Olgica Ceric, Gamola Z. Fortenberry, Beth Harris, Sheryl Shaw, Glenn Tillman, Shaohua Zhao, Uday Dessai
Summary: This study used genomic data to assess potential sources of ExPEC strains and found a higher likelihood of human infection-related strains in food animals and companion animals. Additionally, strains in high relative risk groups were more commonly resistant to antimicrobial drugs. This study highlights the importance of a One Health approach in identifying and managing human pathogens.
Article
Immunology
Jin Hu, Dongfang Wang, Xingfa Huang, Yang Yang, Xin Lian, Wenjun Wang, Xiao Xu, Yulan Liu
Summary: This study explores the role of TolC in the pathogenesis of ExPEC strain PPECC42 and finds that TolC is closely associated with the virulence of ExPEC.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Infectious Diseases
Catia Longhi, Linda Maurizi, Antonietta Lucia Conte, Massimiliano Marazzato, Antonella Comanducci, Mauro Nicoletti, Carlo Zagaglia
Summary: Multiple-antibiotic-resistant extra-intestinal pathogenic Escherichia coli (ExPEC) is a common cause of nosocomial and community-acquired infections. The coexistence of antibiotic and heavy metal resistance in ExPEC strains is increasing, and the mechanisms for heavy metal tolerance are not fully understood.
Article
Public, Environmental & Occupational Health
Wan-Ling Chen, Ming-Fang Cheng, Fan-Chen Tseng, Pin-Chien Wu, I-Fei Huang, Yun-Wen Chen, Yee-Hsuan Chiou, Yao-Shen Chen, Susan Shin-Jung Lee, Wan-Yu Hung, Jiun-Ling Wang, Chih-Hsin Hung
Summary: In this study, the distribution and resistance mechanisms of ESBL-producing E. coli strains were investigated in 120 children. The analysis revealed that these strains were mainly found in densely populated urban areas, with ST131 being the most challenging group. Therefore, the importance of enhancing environmental surveillance and sanitary procedures is emphasized.
JOURNAL OF INFECTION AND PUBLIC HEALTH
(2023)
Article
Microbiology
Magdalena Ksiezarek, Angela Novais, Luisa Peixe
Summary: This study explored the population diversity and antimicrobial resistance of E. coli in the urogenital microbiome and evaluated their genomic relationship with ExPEC strains from healthy and diseased hosts. It found that the healthy urogenital microbiome could be a source of potentially pathogenic and antibiotic resistant E. coli strains.
Article
Infectious Diseases
Virginia Nunez-Samudio, Maydelin Pecchio, Gumercindo Pimentel-Peralta, Yohana Quintero, Mellissa Herrera, Ivan Landires
Summary: The study conducted in two hospitals in central Panama identified ESBL-producing E. coli strains as a significant public health concern in Central America, with the pandemic clone ST43/ST131 carrying CTX-M-15. This is the first report confirming the presence of this antimicrobial-resistant organism in inpatients and outpatients in the region, highlighting potential challenges in treating infections.
Article
Microbiology
Andre Becker S. Saidenberg, Arnoud H. M. van Vliet, Marc Stegger, Thor Bech Johannesen, Torsten Semmler, Marcos Cunha, Alessandro C. de O. Silveira, Eleine Kuroki Anzai, Isabel C. A. Scaletsky, Anders Dalsgaard, Roberto M. La Ragione, Terezinha Knobl
Summary: Extraintestinal pathogenic Escherichia coli (ExPEC) is a globally distributed pathogen associated with human and companion animal diseases, while avian pathogenic pathotype (APEC) severely impacts poultry health and production. The similarities between APEC and human ExPEC suggest a potential for zoonotic transmission.
VETERINARY MICROBIOLOGY
(2022)
Article
Veterinary Sciences
Virginie Guerin, Alban Farchi, Frederic Cawez, Paola Mercuri, Pierrick Lucas, Yannick Blanchard, Marc Saulmont, Jacques Mainil, Damien Thiry
Summary: Escherichia coli producing Extended-Spectrum-beta-Lactamases (ESBL) are a major global public health hazard. This study aimed to determine the prevalence of ESBL in pathogenic and non-pathogenic E. coli isolated from young diarrheic and septicaemic calves over three calving seasons. The results showed a gradual decrease in ESBL-resistance in pathogenic E. coli, with no significant difference in genetic background or CTX-M content between pathogenic and non-pathogenic E. coli.
RESEARCH IN VETERINARY SCIENCE
(2022)
Review
Public, Environmental & Occupational Health
Rajlakshmi Viswanathan, Alok Chakrabarty, Sulagna Basu
Summary: In the aftermath of a natural disaster, microbiologists play a crucial role in multispecialty rapid response teams by providing laboratory diagnosis, situational analysis, evaluation, planning, prevention and control support. Their tasks include risk assessment for identifying epidemic and endemic diseases, evaluation of existing laboratory setups, establishment of services, training of laboratory staff, and focus on infection prevention and control. Their expertise proves valuable in providing timely and useful support for infectious disease management in disaster-affected areas.
TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE
(2021)
Article
Microbiology
Sharmi Naha, Kirsty Sands, Subhankar Mukherjee, Bijan Saha, Shanta Dutta, Sulagna Basu
Summary: This study characterized carbapenemase-producing isolates in an Indian neonatal unit in terms of their resistome, transmissibility, and genome diversity. It identified diverse sequence types and highlighted the extent of plasmid transmission across different strains. The study also found the coexistence of different carbapenemase genes in these neonatal isolates, emphasizing the need for prompt attention to neonatal health care in the face of antimicrobial resistance.
Article
Microbiology
Subhasree Roy, Somdatta Chatterjee, Amrita Bhattacharjee, Pinaki Chattopadhyay, Bijan Saha, Shanta Dutta, Sulagna Basu
Summary: This study found high resistance of neonatal septicaemic Acinetobacter baumannii to fluoroquinolones, particularly moxifloxacin, with multiple active resistance mechanisms, including overexpression of efflux pumps. The diversity of strains and the presence of novel sequence types indicate a complex epidemiological relationship, while mutations within GyrA, ParC, and efflux pump regulators play a critical role in resistance.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Kirsty Sands, Maria J. Carvalho, Edward Portal, Kathryn Thomson, Calie Dyer, Chinenye Akpulu, Robert Andrews, Ana Ferreira, David Gillespie, Thomas Hender, Kerenza Hood, Jordan Mathias, Rebecca Milton, Maria Nieto, Khadijeh Taiyari, Grace J. Chan, Delayehu Bekele, Semaria Solomon, Sulagna Basu, Pinaki Chattopadhyay, Suchandra Mukherjee, Kenneth Iregbu, Fatima Modibbo, Stella Uwaezuoke, Rabaab Zahra, Haider Shirazi, Adil Muhammad, Jean-Baptiste Mazarati, Aniceth Rucogoza, Lucie Gaju, Shaheen Mehtar, Andre N. H. Bulabula, Andrew Whitelaw, Timothy R. Walsh
Summary: The BARNARDS study analyzed bacterial isolates from neonates with sepsis in LMICs, showing antimicrobial-resistant Klebsiella, Escherichia coli, and Enterobacter as main species. Antimicrobial resistance is rising in neonatal sepsis, with poorly understood mechanisms that limit treatment options in LMICs.
NATURE MICROBIOLOGY
(2021)
Article
Infectious Diseases
Kathryn M. Thomson, Calie Dyer, Feiyan Liu, Kirsty Sands, Edward Portal, Maria J. Carvalho, Matthew Barrell, Ian Boostrom, Susanna Dunachie, Refath Farzana, Ana Ferreira, Francis Frayne, Brekhna Hassan, Ellis Jones, Lim Jones, Jordan Mathias, Rebecca Milton, Jessica Rees, Grace J. Chan, Delayehu Bekele, Abayneh Mahlet, Sulagna Basu, Ranjan K. Nandy, Bijan Saha, Kenneth Iregbu, Fatima Modibbo, Stella Uwaezuoke, Rabaab Zahra, Haider Shirazi, Najeeb U. Syed, Jean-Baptiste Mazarati, Aniceth Rucogoza, Lucie Gaju, Shaheen Mehtar, Andre N. H. Bulabula, Andrew Whitelaw, Johan G. C. van Hasselt, Timothy R. Walsh
Summary: When advocating antibiotic treatments, accessibility and affordability must be considered, tailored to the variance in economic health structures across low- and middle-income countries. Funding comes from The Bill & Melinda Gates Foundation. This article is published under an Open Access license.
LANCET INFECTIOUS DISEASES
(2021)
Article
Microbiology
Priyanka Jain, Rajlakshmi Viswanathan, Gourab Halder, Sulagna Basu, Shanta Dutta
Summary: This report presents the draft whole-genome sequences of two multidrug-resistant strains of Salmonella enterica serovar Senftenberg sequence type 14, which were resistant to ciprofloxacin, ceftriaxone, and/or azithromycin. These strains were isolated from neonatal stool and goat meat in Kolkata, India.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2022)
Article
Infectious Diseases
Sharmi Naha, Kirsty Sands, Suchandra Mukherjee, Shanta Dutta, Sulagna Basu
Summary: This study aimed to understand the susceptibility trend and molecular mechanisms of colistin resistance in neonatal isolates over a 12 year period. The results showed that colistin-resistant Klebsiella pneumoniae had multiple mechanisms of resistance, including overexpression of two-component systems and AcrAB-TolC pump and its regulators. The presence of strains with dual carbapenemases causing sepsis in neonates is alarming.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2022)
Review
Medicine, General & Internal
Subhasree Roy, Goutam Chowdhury, Asish K. Mukhopadhyay, Shanta Dutta, Sulagna Basu
Summary: Acinetobacter baumannii is a leading cause of nosocomial infections due to its ability to acquire antibiotic resistance genes and form biofilms. This review discusses the evolution of antibiotic resistance, mechanisms of biofilm formation, infections associated with A. baumannii colonization on medical devices, and strategies for preventing biofilm formation.
FRONTIERS IN MEDICINE
(2022)
Article
Infectious Diseases
Kirsty Sands, Maria J. Carvalho, Owen B. Spiller, Edward A. R. Portal, Kathryn Thomson, William John Watkins, Jordan Mathias, Calie Dyer, Chinenye Akpulu, Robert Andrews, Ana Ferreira, Thomas Hender, Rebecca Milton, Maria Nieto, Rabaab Zahra, Haider Shirazi, Adil Muhammad, Shermeen Akif, Muhammad Hilal Jan, Kenneth Iregbu, Fatima Modibbo, Stella Uwaezuoke, Grace J. Chan, Delayehu Bekele, Semaria Solomon, Sulagna Basu, Ranjan Kumar Nandy, Sharmi Naha, Jean-Baptiste Mazarati, Aniceth Rucogoza, Lucie Gaju, Shaheen Mehtar, Andre N. H. Bulabula, Andrew Whitelaw, Timothy R. Walsh
Summary: This study analyzed neonatal blood samples from low- and middle-income countries in Africa and South Asia, finding that Staphylococcus aureus is the dominant pathogen causing neonatal sepsis, with some resistance to antibiotics. Further research is needed to understand the species diversity and resistance mechanisms of Gram-positive bacteria and their impact on neonatal outcomes.
BMC INFECTIOUS DISEASES
(2022)
Article
Microbiology
Subhasree Roy, Vivek Junghare, Shanta Dutta, Saugata Hazra, Sulagna Basu
Summary: Resistance-nodulation-division-type efflux system AdeABC plays a crucial role in carbapenem resistance among Acinetobacter baumannii. This study investigates the role of its regulator AdeRS in carbapenem-resistant A. baumannii (CRAB) and explores the interaction of carbapenems and pump inhibitor PA beta N with the AdeB structure. The study identifies AdeRS mutations associated with overexpression of AdeABC and provides insights into the atomic level causes of pump inhibition, facilitating the discovery of novel inhibitors.
Article
Microbiology
M. J. Carvalho, K. Sands, K. Thomson, E. Portal, J. Mathias, R. Milton, D. Gillespie, C. Dyer, C. Akpulu, I Boostrom, P. Hogan, H. Saif, A. Ferreira, M. Nieto, T. Hender, K. Hood, R. Andrews, W. J. Watkins, B. Hassan, G. Chan, D. Bekele, S. Solomon, G. Metaferia, S. Basu, S. Naha, A. Sinha, P. Chakravorty, S. Mukherjee, K. Iregbu, F. Modibbo, S. Uwaezuoke, L. Audu, C. P. Edwin, A. H. Yusuf, A. Adeleye, A. S. Mukkadas, R. Zahra, H. Shirazi, A. Muhammad, S. N. Ullah, M. H. Jan, S. Akif, J. B. Mazarati, A. Rucogoza, L. Gaju, S. Mehtar, A. N. H. Bulabula, A. Whitelaw, L. Roberts, T. R. Walsh
Summary: This study in low-income settings revealed associations between the gut microbiota of mothers and their neonates, discovering a correlation between the carriage of beta-lactamase genes and the risk of neonatal sepsis.
NATURE MICROBIOLOGY
(2022)
Article
Microbiology
Subhankar Mukherjee, Punyasloke Bhadury, Shravani Mitra, Sharmi Naha, Bijan Saha, Shanta Dutta, Sulagna Basu
Summary: This study characterized hvKP/CR-hvKP strains causing neonatal sepsis in terms of resistance and virulence. About 26% of the strains belonged to hvKP, with hvKP-K2 being the prevalent pathotype. The majority of the strains exhibited strong biofilm-forming and high serum resistance ability. Additionally, this study reported for the first time the NDM-1-producing hvKP ST11-K2 and ST15-K54 strains causing fatal neonatal sepsis.
MICROBIOLOGY SPECTRUM
(2023)
Article
Infectious Diseases
Ankur Rao, Sharmi Naha, Amrita Bhattacharjee, Pinaki Chattopadhyay, Shanta Dutta, Sulagna Basu
Summary: The presence and dissemination of plasmid-mediated AmpC genes (pAmpCs) in bacteria have made them cephalosporin-resistant. This study analyzed the prevalence and diversity of pAmpCs in Klebsiella pneumoniae and Escherichia coli isolated from septicaemic neonates. The study found that pAmpCs were present in 9% of strains, with blaCMY-42 and blaDHA-1 variants being predominant. Some strains also co-harbored carbapenemase genes, blaNDM and blaOXA-48.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2023)
Article
Microbiology
Amrita Bhattacharjee, Kirsty Sands, Shravani Mitra, Ritojeet Basu, Bijan Saha, Olivier Clermont, Shanta Dutta, Sulagna Basu
Summary: This study analyzed 80 isolates of Escherichia coli collected from neonates with sepsis between 2009 and 2019. The majority of the isolates were multidrug-resistant and 44% were carbapenem-resistant due to the presence of the bla(NDM) gene. Different phylogroups and clonal complexes were identified, with ST167 and ST131 being the most common epidemic clones.
MICROBIOLOGY SPECTRUM
(2023)
Article
Public, Environmental & Occupational Health
Rebecca Milton, David Gillespie, Calie Dyer, Khadijeh Taiyari, Maria J. Carvalho, Kathryn Thomson, Kirsty Sands, Edward A. R. Portal, Kerenza Hood, Ana Ferreira, Thomas Hender, Nigel Kirby, Jordan Mathias, Maria Nieto, William J. Watkins, Delayehu Bekele, Mahlet Abayneh, Semaria Solomon, Sulagna Basu, Ranjan K. Nandy, Bijan Saha, Kenneth Iregbu, Fatima Z. Modibbo, Stella Uwaezuoke, Rabaab Zahra, Haider Shirazi, Syed U. Najeeb, Jean-Baptiste Mazarati, Aniceth Rucogoza, Lucie Gaju, Shaheen Mehtar, Andre N. H. Bulabula, Andrew C. Whitelaw, Timothy R. Walsh, Grace J. Chan
Summary: This study aimed to determine the incidence and associations with neonatal sepsis and all-cause mortality in facility-born neonates in low-income and middle-income countries (LMICs). Findings reveal a high incidence of neonatal sepsis and its significant impact on mortality rates in LMICs.
LANCET GLOBAL HEALTH
(2022)