期刊
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
卷 181, 期 -, 页码 47-54出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.saa.2017.03.032
关键词
Graphene oxide; L-cys-AgNPs; GO-L-cys-Ag nanocomposite; Antibacterial activity; SEM
类别
资金
- DST-FIST [SR/FF/CS-39/2011]
- UGC-SAP [F.540/7/DRS-II/2016(SAP-I)]
- C-COST,Raipur [1104 CCOST MRP]
- Pt. Ravishanker Shukla University, Raipur [79/12/Fin./2014]
Graphene oxide (GO) sheets decorated with amino acid L-cysteine (L-cys) functionalized silver nanoparticles (GO-L-cys-Ag) was synthesized by AgNO3, trisodium citrate, and NaBH4. GO-L-cys-Ag nanocomposite was characterized by transmission electron microscopy (TEM), Fourier transform infrared (FTIR) spectra, ultraviolet-visible (UV-vis) absorption spectra, which demonstrated that a diameter of L-cys-AgNPs compactly deposited on GO. Antibacterial activity tests of GO-L-cys-Ag nanocomposite were carried out using Escherichia coli MTCC 1687 and Staphylococcus aureus MTCC 3160 as model strains of Gram-negative and Gram-positive bacteria, respectively. The effect of bactericide dosage on antibacterial activity of GO-L-cys-Ag nanocomposite was examined by plate count, well diffusion and broth dilution methods. Morphological observation of bacterial cells by scanning electron microscope (SEM) showed that GO-L-cys-Ag nanocomposite was more destructive to cell membrane of Escherichia coli than that of Staphylococcus aureus. The above technique establish that the bactericidal property of GO-L-cys-Ag nanocomposite with wide range of applications in biomedical science. (C) 2017 Elsevier B.V. All rights reserved.
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