Article
Biochemistry & Molecular Biology
Andres H. Morales, Johan S. Hero, Ana E. Ledesma, M. Alejandra Martinez, Maria C. Navarro, Maria I. Gomez, Cintia M. Romero
Summary: This study investigates the effects of pH and ionic strength fluctuations on protein-support interactions during lipase immobilization. The optimal immobilization conditions do not necessarily result in the highest hydrolytic activity. Adsorption isotherm analysis confirms this observation. Furthermore, the study demonstrates the interfacial hyperactivation of the lipase towards acidic pH and highly charged environments, and examines the mechanism of surface hydrophobic protein-carrier interactions.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Energy & Fuels
Fareeha Nadeem, Tahir Mehmood, Zahid Anwar, Shagufta Saeed, Muhammad Bilal, Bisma Meer
Summary: This study demonstrated the maximum production of lipase via solid-state fermentation using lignocellulosic biomass. The optimized conditions led to a high yield of lipase, which was successfully immobilized on chitosan and showed potential for applications in the detergent industry.
BIOMASS CONVERSION AND BIOREFINERY
(2021)
Article
Chemistry, Applied
Sara Arana-Pena, Nathalia S. Rios, Diego Carballares, Luciana R. B. Goncalves, Roberto Fernandez-Lafuente
Summary: CALB and CRL showed similar properties under different immobilization conditions, while RML displayed significant changes. The effects of immobilization conditions on substrate activity and stability vary greatly, requiring careful selection based on specific circumstances.
Article
Chemistry, Multidisciplinary
Itoh Toshiyuki
Summary: The use of ILs as solvents and stabilizers for enzymes, specifically lipases, allows for the recyclable use of enzymes, acceleration of transesterification, and enhancement of enzyme stability and reaction activity. This IL engineering approach has a significant impact on lipase-catalyzed reactions and has the potential to improve enzyme capabilities.
Article
Biotechnology & Applied Microbiology
Shushu Wang, Shan Li, Runtang Liu, Wei Zhang, Huajin Xu, Yi Hu
Summary: In this study, Candida rugosa lipase (CRL) was activated and covalently immobilized on a nanocomposite material using surfactants. The nanocomposite material, fabricated by combining magnetic nanoparticles with chitosan, proved to be an ideal support for enzyme immobilization. The immobilized CRL showed improved stability and reusability.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biophysics
Hongbo Suo, Moju Li, Renmin Liu, Lili Xu
Summary: In this study, imidazolium-based ionic liquid was successfully grafted to magnetic polydopamine nanoparticles for lipase immobilization. The immobilized lipase showed excellent activity and stability, with increased affinity to substrate. Additionally, the immobilized lipase can be easily separated from the reaction system with a magnet, providing new ideas for further studies in this field.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Article
Biochemistry & Molecular Biology
Zeping Lu, Mei Chen, Tongtong Jin, Binbin Nian, Yi Hu
Summary: Ionic liquids (ILs) are used as chemical modifiers to improve the efficiency, activity, and stability of enzymes. This study investigated the impact of ILs with different chain lengths on the enzymatic properties of Candida antarctica lipase B (CALB) and found that ILs significantly influenced CALB's absorption orientation. Molecular dynamics simulations showed that ILs with suitable chain lengths formed strong interactions with CNTs and ILs, stabilizing CALB's open state and improving its activity and stability. This study provides insights into the design of green modifiers for better immobilized enzymes and lays the groundwork for future research on IL modifiers.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Chemistry, Physical
Balaji Panchal, Yongjing Hao, Zhibin Han, Tao Chang, Zheng Zhu, Xionglei Wang, Shenjun Qin
Summary: In this study, fatty acid esters were efficiently synthesized using a reusable lipase immobilized with a novel porous ionic polymer. The immobilized lipase showed excellent catalytic activity and stability, making it a cost-effective and environmentally friendly catalyst for biodiesel production.
JOURNAL OF CATALYSIS
(2022)
Article
Biochemistry & Molecular Biology
Moju Li, Xusheng Dai, Aifeng Li, Qi Qi, Wenhui Wang, Jia Cao, Zhenting Jiang, Renmin Liu, Hongbo Suo, Lili Xu
Summary: In this study, an ionic liquid-modified magnetic metal-organic framework composite was prepared and used as a support for enzyme immobilization. The immobilized enzyme showed higher catalytic activity, thermal stability, and anti-denaturant properties compared to soluble enzymes. The results demonstrated that the ionic liquid-modified Fe3O4@UiO-66-NH2 has great potential for immobilized enzymes.
Article
Chemistry, Applied
Lili Xu, Xinyue Geng, Rui Cao, Jiahao Zhu, Fei Chen, Cuihong Li, Lexuan Wang, Xuekun Wang, Hongbo Suo
Summary: By preparing magnetic MOFs and using an ionic liquid as a linker, enzyme immobilization was successfully achieved. The immobilized enzyme showed excellent activity and stability, with high efficiency in recycling and reusability. This research provides a meaningful reference for the application of other MOF composites in the field of enzyme immobilization.
MICROPOROUS AND MESOPOROUS MATERIALS
(2023)
Article
Green & Sustainable Science & Technology
Zhijin Zhang, Yingjie Du, Geling Kuang, Xuejian Shen, Xiaotong Jia, Ziyuan Wang, Yuxiao Feng, Shiru Jia, Fufeng Liu, Muhammad Bilal, Jiandong Cui
Summary: A novel lipase hybrid biocatalyst with superior catalytic activity and stability was developed through dual activation of calcium ions and deep-eutectic solvents. Compared to free lipase, this catalyst exhibited improved substrate selectivity, tolerance to chemical denaturants, and reusability.
Article
Biotechnology & Applied Microbiology
Anna Wolny, Agnieszka Siewniak, Jakub Zdarta, Filip Ciesielczyk, Piotr Latos, Sebastian Jurczyk, Long D. Nghiem, Teofil Jesionowski, Anna Chrobok
Summary: In this study, supported ionic liquid phase (SILP) was used as a carrier for lipase from Aspergillus oryzae (LAO) to catalyze the enantiomeric resolution of racemic ibuprofen. The results showed high conversion of racemic ibuprofen and high enantiomeric excess (ee) of the desired enantiomer, (S)-(+)-ibuprofen ester. This approach has significant potential for industrial applications.
ENVIRONMENTAL TECHNOLOGY & INNOVATION
(2022)
Article
Nanoscience & Nanotechnology
Min Wu, Song Chen, Yang Mei, Lan Liu, Yong Wei
Summary: By incorporating ionic liquids and soft polyethylene glycol into a rigid epoxy precursor and curing, researchers have developed adhesives with high initial bonding strength and rapid detaching efficiency, showcasing a new approach for the design of fast-detachable adhesives.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Chemistry, Physical
Qian Wang, Limei Sheng, Xuan Guo, Rong Chen, Chengjie Zhou, Fu Yang
Summary: This paper presents the use of functional polymers as carriers for enzymes and noble metals in the DKR reaction. By modulating the weak forces of the polymers, the catalysts exhibited synergistic effects and long-term stability, leading to high conversion and optical purity of the products. Furthermore, the catalysts could be easily recovered and recycled with high efficiency.
APPLIED CATALYSIS A-GENERAL
(2023)
Article
Chemistry, Multidisciplinary
Andres H. Morales, Johan S. Hero, Ana E. Ledesma, Hugo A. Perez, Maria C. Navarro, Maria I. Gomez, Cintia M. Romero
Summary: This study provides a comprehensive investigation on the adsorption process of Candida rugosa lipase (CRL) on Ca2Fe2O5 iron oxide nanoparticles (NPs). Protein-support interactions were analyzed through various methods, including response surface methodology, surface charge determination, spectroscopic and in silico analyses. The results revealed the optimal conditions for maximum protein immobilization differed from those for maximum hydrolytic activity. Electrostatic and hydrophobic interactions were found to contribute to protein-support binding. This research contributes to our understanding of the design of biocatalysts immobilized in iron oxide NPs.
Review
Biotechnology & Applied Microbiology
Priscila Esteves Faria, Aline M. Castro, Denise Maria Guimaraes Freire, Rafael D. Mesquita
Summary: This article elucidates the details of isomer production of 2,3-butanediol and acetoin, non-oxidative metabolism products of microorganisms, through bioinformatics analysis and compares the stereo-triads of other enzymes. These results are of great importance for understanding the biosynthesis and industrial production of BD/AC.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2023)
Article
Polymer Science
Martina C. C. Pinto, Isabelly Sousa, Luciana Dutra, Stefanni S. Everton, Jaqueline Greco-Duarte, Eliane Pereira Cipolatti, Erika Cristina G. Aguieiras, Evelin A. Manoel, Denise Maria G. Freire, Jose Carlos Pinto
Summary: This study focuses on developing new enzymatic biocatalysts for esterification and transesterification reactions by synthesizing polymer supports with distinct functional compounds. Different polymerization strategies were used to synthesize the supports, leading to particles with high surface areas and highly active biocatalysts that achieved esterification conversions above 80%. The new functional matrices showed great potential for use in the biodiesel industry based on their performance in esterification and transesterification reactions.
JOURNAL OF APPLIED POLYMER SCIENCE
(2022)
Article
Engineering, Chemical
Stefanni S. Everton, Isabelly Sousa, Luciana da Silva Dutra, Eliane Pereira Cipolatti, Erika Cristina G. Aguieiras, Evelin Andrade Manoel, Jaqueline Greco-Duarte, Martina C. C. Pinto, Denise Maria Guimaraes Freire, Jose Carlos Pinto
Summary: The research on enzymatic production of biodiesel in Brazil has made progress, with most studies conducted in the southeast region, while biodiesel production is concentrated in the middle western states. The use of biomass as feedstock or raw material for biocatalysts can make the production of biodiesel more sustainable and economically viable.
BRAZILIAN JOURNAL OF CHEMICAL ENGINEERING
(2023)
Article
Biochemistry & Molecular Biology
Aluisio Marques da Fonseca, Antonio Luthierre Gama Cavalcante, Antonia Mayara dos Santos Mendes, Francisco Danilo Ferreira Costa da Silva, Debora Cristina Lima Ferreira, Paulo Riceli Vasconcelos Ribeiro, Jose Cleiton Sousa dos Santos, Helcio Silva dos Santos, Eduardo Menezes Gaieta, Gabrielle Silva Marinho, Regilany Paulo Colares, Emmanuel Silva Marinho
Summary: The methanolic extract of L. camara flowers showed toxicity, antioxidant activity, and acetylcholinesterase activity. The extract was found to contain various polyphenolic compounds, including phenolic acid derivatives, phenylethanoid glycosides, and flavonoids. In silico simulation also showed that the flavonoid isoverbascoside had a strong affinity for the AChE enzyme. These findings suggest that the phytochemical content of L. camara extracts, particularly the presence of flavonoids and phenolic compounds, may contribute to their antioxidant and anticholinesterase potential.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Review
Chemistry, Physical
Batista Dala Catumba, Misael Bessa Sales, Pedro Tavares Borges, Manoel Nazareno Ribeiro Filho, Ada Amelia Sanders Lopes, Maria Alexsandra de Sousa Rios, Ajay S. Desai, Muhammad Bilal, Jose Cleiton Sousa dos Santos
Summary: Hydrogen production has a wide range of feedstocks and methods, and research in this field has been growing and diversifying. This study analyzed over 10,655 publications from 2010 to 2022 and identified the top organizations, journals, authors, and literature in hydrogen production research. The findings showed that the Chinese Academy Of Sciences, Ontario Tech University, and Xi An Jiaotong University were the organizations with the highest number of publications. The International Journal Of Hydrogen Energy had the most publications. The study also highlighted the research focus areas of photocatalytic hydrogen production and fermentative hydrogen production.
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
(2023)
Review
Biochemistry & Molecular Biology
Antonio F. S. Rodrigues, Ananias F. da Silva, Francisco L. B. da Silva, Kaiany M. dos Santos, Marcelo P. de Oliveira, Millena M. R. Nobre, Batista D. Catumba, Misael B. Sales, Allison R. M. Silva, Ana Katia S. Braz, Antonio L. G. Cavalcante, Jeferson Y. N. H. Alexandre, Paulo G. S. Junior, Roberta B. R. Valerio, Viviane de Castro Bizerra, Jose C. S. dos Santos
Summary: This article conducted an advanced and comprehensive scientometric analysis of worldwide trends in decolorizing synthetic dyes by laccase. China, India, and Turkey were identified as the main contributors to this research theme. The results of the presented analysis encompassed data generated from 1999 to 2021, spanning more than two decades of specialized research.
PROCESS BIOCHEMISTRY
(2023)
Review
Polymer Science
Francisco Simao Neto, Maria Marliete Fernandes de Melo Neta, Misael Bessa Sales, Francisco Arisson Silva de Oliveira, Viviane de Castro Bizerra, Ada Amelia Sanders Lopes, Maria Alexsandra de Sousa Rios, Jose Cleiton Sousa dos Santos
Summary: Lignocellulosic biomasses are widely used in various applications, leading to increased consumption and waste generation. Research is being conducted to explore alternative uses and examine the use of these biomasses as enzymatic support. Advanced bibliometric analysis is employed to identify the biomasses used and other research perspectives in this field.
Article
Chemistry, Physical
Joao Brandao Junior, Jean Gleison Andrade do Nascimento, Michael Pablo Franca Silva, Eliane de Aquino Lima Brandao, Viviane de Castro Bizerra, Kaiany Moreira dos Santos, Juliana de Franca Serpa, Jose Cleiton Sousa dos Santos, Aluisio Marques da Fonseca, Diego Lomonaco Vasconcelos de Oliveira, Maria Cristiane Martins de Souza
Summary: In this study, biodiesel was produced from vegetable oils through enzymatic esterification. The results showed that the enzyme catalysts had better performance compared to chemical catalysts.
Article
Biotechnology & Applied Microbiology
Paulo Goncalves de Sousa Junior, Ana Gabrielle do Nascimento Camara, Artur Ramon Tome Oliveira, Gabriella de Castro Lima, Gledson Vieira Lima, Lucas Pinheiro Coutinho, Jeferson Yves Nunes Holanda Alexandre, Leonardo F. Serafim, Marcos Carlos de Mattos, Norberto Vieira de Kassio Monteiro, Jose Cleiton Sousa dos Santos
Summary: Eversa & REG Transform 2.0 is a genetically modified lipase derived from Thermomyces lanuginosus, which has a low production cost and exhibits high substrate specificity and catalytic activity. The optimal conditions for enzymatic esterification of glycerol with acetic acid were determined using the Taguchi method, and a yield of 84.8% was achieved. Temperature was found to be the most significant parameter influencing the reaction conversion. The mechanism of esterification was investigated through theoretical studies, revealing that the nucleophilic attack step is the rate-limiting step.
BIOCHEMICAL ENGINEERING JOURNAL
(2023)
Article
Engineering, Chemical
Francisco Simao Neto, Maria Melo Neta, Ana Sousa, Luana Damasceno, Bruna Sousa, Samuel Medeiros, Rafael Melo, Ada Lopes, Jose Santos, Maria Rios
Summary: The energetic potential of the seed shell of the Neem plant was investigated using various analytical techniques, and the results showed its suitability as a biofuel due to its composition and thermal behavior. The XRF analysis further revealed the presence of several essential elements in the seed shell.
Article
Biotechnology & Applied Microbiology
Gabriela C. Breda, Priscila E. Faria, Yuri S. Rodrigues, Priscila B. Pinheiro, Maria Clara R. Nucci, Pau Ferrer, Denise M. G. Freire, Rodrigo V. Almeida, Rafael D. Mesquita
Summary: ProspectBIO is a new software for bioprospecting that can efficiently find non-annotated enzyme sequences by searching for homologs in genomes. Using this software, a novel lipase-encoding gene from Ustilago hordei was discovered and successfully expressed in yeast, resulting in an active enzyme.
Article
Engineering, Environmental
Isamayra Germano de Sousa, Gabrielly Ferreira Mota, Antonio Luthierre Gama Cavalcante, Thales Guimaraes Rocha, Patrick da Silva Sousa, Jeferson Yves Nunes Holanda Alexandre, Jose Erick da Silva Souza, Francisco Simao Neto, Francisco Thalysson Tavares Cavalcante, Ada Amelia Sanders Lopes, Jose C. S. dos Santos
Summary: In recent years, there has been an increasing demand for sustainable energy resources, prompting researchers worldwide to develop green technologies and products. Biolubricants have emerged as a viable alternative to petroleum-derived lubricants, being biodegradable, non-toxic, and easily disposable in the environment. Enzymatic biocatalysts, particularly lipases, offer a promising alternative for biolubricant production, as they generally exhibit higher energy efficiency and produce less waste compared to conventional chemical catalysts. This article discusses various aspects of lipase-catalyzed biolubricant production, including different reactions, raw material sources, and alternative process protocols such as enzymatic immobilization, alcohols, and bioreactors.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2023)
Article
Chemistry, Multidisciplinary
Paula J. M. Lima, Jouciane de S. Silva, Rafael L. F. Melo, Francisco S. Neto, Pierre B. A. Fechine, Maria V. P. Rocha, Luciana R. B. Goncalves, Jose C. S. dos Santos
Summary: In this study, chemically modified cashew apple bagasse (CAB) was used as a support for immobilizing Candida antarctica lipase A (CAL-A). The optimal activation conditions for CAB support were determined using advanced experimental design. The modified CAB showed improved thermal stability at different temperatures and pH values.
Review
Chemistry, Applied
Roberta Bussons Rodrigues Valerio, Antonio Luthierre Gama Cavalcante, Gabrielly Ferreira Mota, Isamayra Germano de Sousa, Jose Erick da Silva Souza, Francisco Thalysson Tavares Cavalcante, Katerine da Silva Moreira, Italo Rafael de Aguiar Falcao, Francisco Simao Neto, Jose C. S. dos Santos
Summary: This review presents the characteristics and potential of Rhizopus chinensis lipases in industrial applications, highlighting their catalytic role in esterification reactions in solvent-free systems. It also discusses the immobilization techniques and main biocatalytic reactions of these enzymes. The study shows that these lipases have ample potential for various biotechnological applications due to their economic feasibility and environmental friendliness.
BIOINTERFACE RESEARCH IN APPLIED CHEMISTRY
(2022)
Article
Biotechnology & Applied Microbiology
Thore Bach Thomsen, Tobias S. Radmer, Anne S. Meyer
Summary: Plastic pollution, particularly from PET, is a significant environmental challenge. This study investigates the enzyme kinetics of PET degradation and finds that the degree of crystallinity (X-C) has a substantial impact on the enzymatic reaction rate. The mobile amorphous fraction (X-MAF) is a better descriptor for the rate of enzymatic degradation. The longer lag phase observed with increased X-C is proposed to be due to increased substrate entanglement density and unproductive enzyme binding to the crystalline regions of PET.
ENZYME AND MICROBIAL TECHNOLOGY
(2024)
Article
Biotechnology & Applied Microbiology
Purna Bahadur Poudel, Dipesh Dhakal, Jong Cheol Lee, Jae Kyung Sohng
Summary: Methylation plays important roles in various biological processes, and this study characterized the function of ThnM3 in regioselective modifications of 1,8-dihydroxynaphthalene.
ENZYME AND MICROBIAL TECHNOLOGY
(2024)
Article
Biotechnology & Applied Microbiology
Feng Song, Ivan Birukou, Mark Bednarcik, Scott Young
Summary: In this study, a highly sensitive and specific method using ultra performance liquid chromatography (UPLC) was developed to measure the enzymatic activity of APH4 in plants. The method exhibited good precision and accuracy, and was validated for use with samples from different sources.
ENZYME AND MICROBIAL TECHNOLOGY
(2024)
Article
Biotechnology & Applied Microbiology
Yuan Rao, Wei Zhuang, Jinle Liu, Ting Tang, Zhi Wang, Hanjie Ying
Summary: This study describes a mild and versatile strategy to immobilize glucose oxidase (GOD) on carboxyl-activated metal-organic framework (MOF) through DNA-directed immobilization technology. The immobilized GOD showed enhanced stability and catalytic activity, making it a potential candidate for applications in biocatalysis and biotechnology.
ENZYME AND MICROBIAL TECHNOLOGY
(2024)
Article
Biotechnology & Applied Microbiology
Julen Ordenana Manso, Martin B. Nielsen, Eva Balaguer Moya, Juliana P. Sandri, Celina K. Yamakawa, Solange I. Mussatto
Summary: This study focuses on the development of an enzymatic cocktail for the conversion of corn fiber into monosaccharides. The results reveal that the recalcitrance and unique chemical composition of corn fiber require a tailored enzymatic cocktail for degradation, and attention should also be given to the pretreatment step.
ENZYME AND MICROBIAL TECHNOLOGY
(2024)
Article
Biotechnology & Applied Microbiology
Cuixia Zhou, Guangcheng Yang, Panpan Meng, Weishuai Qin, Yanyan Li, Zhenxian Lin, Wei Hui, Huitu Zhang, Fuping Lu
Summary: Bacillus licheniformis 2709 is an important industrial producer of alkaline protease (AprE), and this study identified the UP elements of the aprE gene and demonstrated the lack of significant effect of CggR, FruR, and YhcZ transcription factors on aprE expression. Enhanced aprE expression and enzyme activity were achieved by deleting a negative regulatory factor binding site in the genome. These findings provide valuable insights into the high-level production of proteins in other Bacillus species.
ENZYME AND MICROBIAL TECHNOLOGY
(2024)
Article
Biotechnology & Applied Microbiology
Xinqi Xu, Tianheng Chen, Lian Xu, Juan Lin
Summary: In this study, laccase was immobilized on modified magnetic nanoparticles, improving the enzyme's thermostability and efficiency for polymerization of phenols.
ENZYME AND MICROBIAL TECHNOLOGY
(2024)
Article
Biotechnology & Applied Microbiology
Handan Ustukarci, Gul Ozyilmaz, Ali Tuncay Ozyilmaz
Summary: A biofilm layer called biofouling is formed over time by organic molecules and microorganisms on solid surfaces immersed in a liquid medium. This study focused on the antifouling performance of polyaniline (PANI) and investigated the immobilization of hydrolytic enzymes in PANI coatings. The results showed that the alpha-amylase-containing film exhibited the highest antifouling activity. Surface properties and biofilm composition were analyzed to support these findings.
ENZYME AND MICROBIAL TECHNOLOGY
(2024)
Article
Biotechnology & Applied Microbiology
Mariana Ortiz-Sanchez, Juan Camilo Solarte-Toro, Pablo Jose Inocencio-Garcia, Carlos Ariel Cardona Alzate
Summary: This study evaluated the sustainability of biorefineries using orange peel as a raw material. By applying the concept of biorefineries and experimental data, it was found that steam distillation and polyphenolic compound extraction can improve the process performance, resulting in higher yields of different products. The integrated utilization of orange peel in biorefineries showed a high sustainability index.
ENZYME AND MICROBIAL TECHNOLOGY
(2024)