期刊
ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY
卷 34, 期 5, 页码 1045-1055出版社
WILEY
DOI: 10.1002/etc.2884
关键词
Copper; Mitosis; Microtubule; DNA damage; DNA synthesis
资金
- National Natural Science Foundation of China [31070242]
- Outstanding Scientists Project of Guangdong Provincial Government
- Guangdong Pearl River Scholar Funded Scheme
- Research Fund for the Doctoral Program of Higher Education of China [20114407110006]
- Scientific Research Foundation of Graduate School of South China Normal University [2012kyjj114]
Copper (Cu) is considered to be an indispensable microelement for plants. Excessive Cu, however, is toxic and disturbs several processes in the plant. The present study addressed the effects of ionic Cu (2.0 mu M and 8.0 mu M) on mitosis, the microtubule cytoskeleton, and DNA in root tip cells of Allium cepa var. agrogarum L. to better understand Cu toxicity on plant root systems. The results indicated that Cu accumulated in roots and that root growth was inhibited dramatically in Cu treatment groups. Chromosomal aberrations (for example, C-mitosis, chromosome bridges, chromosome stickiness, and micronucleus) were observed, and the mitotic index decreased during Cu treatments at different concentrations. Microtubules were one of the target sites of Cu toxicity in root tip meristematic cells, and Cu exposure substantially impaired microtubule arrangements. The content of -tubulin decreased following 36h of exposure to 2.0 mu M or 8.0 mu M of Cu in comparison with the control group. Copper increased DNA damage and suppressed cell cycle progression. The above toxic effects became more serious with increasing Cu concentration and prolonged exposure time. Environ Toxicol Chem 2015;34:1045-1055. (c) 2015 SETAC
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