期刊
ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY
卷 34, 期 9, 页码 2087-2094出版社
WILEY-BLACKWELL
DOI: 10.1002/etc.3032
关键词
Japanese medaka; Intersex; Estrogenic compounds; Androgens; Endocrine-disrupting compounds
资金
- Department of Forestry and Natural Resources at Purdue University
- Cultural Affairs and Mission Sector at the Egyptian Ministry of Higher Education
- Grant Agency of the Czech Republic (GACR) [P503/13/34049P]
Intersex in gonochoristic fish can be induced after exposure to androgens and estrogens. The main objective of the present study was to identify biomarkers that would be predictive of intersex in Japanese medaka (Oryzias latipes) after exposure to synthetic hormones. First a gene was identified, ovarian structure protein 1 (osp1), with strong female-specific expression during gonadal differentiation. The authors hypothesized that osp1 expression would decrease to male levels in females after the exposure of larvae (15-25 d postfertilization [dpf]) to 17-trenbolone (TRB; 5ng/L) and would increase to female levels in males exposed to 17-ethinylestradiol (EE2; 5ng/L) and that gonadal intersex would be induced later in life (60 dpf). Tissue distribution and cellular localization of OSP1 was investigated using Western blot and immunohistochemistry. The results indicate that this exposure regime delays testicular maturation in males and development of ovarian intersex in females. Although decreased osp1 expression in females exposed to TRB correlated to changes in ovarian phenotype, up-regulation of osp1 was not observed in males exposed to EE2. In addition, OSP1 was only observed in ovaries and localized in the cytoplasm and follicular layer of immature and mature oocytes. The authors conclude that osp1 is a promising biomarker of androgen exposure and gonadal intersex in female medaka. Environ Toxicol Chem 2015;34:2087-2094. (c) 2015 SETAC
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据