Article
Chemistry, Analytical
Yujie Sun, Qi Dong, Huan Yang, Weiling Song, Hong Zhou
Summary: In this study, a ratiometric electrochemical biosensor based on CuS quantum dots (CuS QDs) regulated by DNAzyme was developed for telomerase detection. Telomerase activity was measured by extending the substrate probe with repeated sequence, releasing CuS QDs as an input to the DNAzyme-modified electrode. The detection range was 1.0 x 10-12 - 1.0 x 10-6 IU/L, with a limit of detection of 2.75 x 10-14 IU/L. Telomerase activity in HeLa extracts was also tested to demonstrate its clinical application.
ANALYTICA CHIMICA ACTA
(2023)
Article
Spectroscopy
Xiaoping Chen, Jianwei Lin, Yafeng Zhuang, Siqi Huang, Jinghua Chen, Zhizhong Han
Summary: A new ratiometric fluorescence sensor is developed for the selective detection of chlorotetracycline (CTC) using dual-mode fluorescence method, demonstrating good selectivity and sensitivity.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Multidisciplinary Sciences
Xiang Zhang, Yu Gu, Yun Zhang, Guo-Yin Yu, Zhi-Peng Liao, Hui-Fang Wu, Chuan-Guo Shi
Summary: In this study, dual-emissive N-GOQDs fluorescent probes were successfully developed for monitoring intracellular pH. These probes possess bright fluorescence, high stability, and good biocompatibility.
Article
Chemistry, Analytical
Yudie Sun, La Fang, Yunxiang Han, Aobo Feng, Shengjun Liu, Kui Zhang, Jing-Juan Xu
Summary: Myocardial miRNAs in peripheral blood play a crucial role in the pathogenic process of myocardial infarction. Researchers have developed a ratiometric electrochemiluminescence biosensor for the rapid identification and accurate quantification of myocardial miRNAs, which has significant implications in clinical interventions for treating cardiovascular lesions.
ANALYTICAL CHEMISTRY
(2022)
Article
Materials Science, Multidisciplinary
Amadeo Sena-Torralba, Helena Torne-Morato, Claudio Parolo, Saba Ranjbar, Mohammad Amin Farahmand Nejad, Ruslan Alvarez-Diduk, Andrea Idili, Mohammad Reza Hormozi-Nezhad, Arben Merkoci
Summary: The majority of lateral flow assays use single-color labels, but this method has two limitations: it is prone to misinterpretation and does not allow precise modulation of sensitivity and dynamic range. To overcome these limitations, a ratiometric approach is developed that allows for adjustment of the assay's analytical properties.
ADVANCED MATERIALS TECHNOLOGIES
(2022)
Article
Biochemical Research Methods
Hanqiang Zhang, Yufei Li, Haixin Lu, Feng Gan
Summary: A new dual-mode ratiometric fluorescence and colorimetric probe has been developed for selective determination of Cu2+. The probe shows high accuracy and sensitivity, and has immense potential for practical applications.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Guomin Yang, Ying He, Jinwen Zhao, Shihong Chen, Ruo Yuan
Summary: This study prepared water-soluble Ir nanorods and developed a highly sensitive dual-signal nanoprobe for detecting organophosphorus pesticides, utilizing the opposite changes in two ECL signals for target detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Spectroscopy
Xinyu Yang, Jiayi Li, Xinhui Tan, Xuejiao Yang, Ping Song, Dengming Ming, Yaqiong Yang
Summary: A portable instrument-free detection method for lipopolysaccharide (LPS) analysis was developed based on a dual-emission ratiometric fluorescence sensing system. The method utilized red-emitting Au nanoclusters (Au NCs) as a reference probe and blue-emitting fluorescent silica quantum dots (Si QDs) as a response probe. The aptamer of LPS was grafted to the surface of Si QDs for specific recognition of LPS. The method showed a linear equation in the range of 50-3000 ng/mL, with a limit of detection (LOD) of 29.3 ng/mL. The method was successfully applied for analyzing LPS in normal saline with a recovery rate of 97.7-103.8%. The detection picture was transformed to Red, Green, and Blue (RGB) using a color picker platform in a smartphone for visual detection of LPS. This low-cost and easy-carry method has broad merits for visually quantitative detection of LPS.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Chemistry, Applied
Bo Peng, Chunjuan Wang, Xueyan He, Yongjun Ma, Min Zhou, Xin Ma, Shengguo Zhao, Yanjun Fang
Summary: A triple-mode colorimetric and fluorescent sensing scheme based on manganese dioxide nanoparticles (MnO2NPs) and carbon quantum dots (CQDs) was developed for nitrite determination. The proposed methods, including UV-vis detection mode and fluorescent detection mode using a smartphone-based measurement, showed accurate and convenient detection of nitrite in food and water samples. The results were validated with the AOAC standard spectrophotometric method.
Article
Engineering, Environmental
Shikha Jain, Neeraj Dilbaghi, Nitin Kumar Singhal, Ajeet Kaushik, Ki-Hyun Kim, Sandeep Kumar
Summary: Accumulation of heavy metal ions in the ecosystem is a major concern for their negative effects on animals, humans, and aquatic habitats. A rapid and portable sensing system is crucial for detecting and managing such pollutants. A fluorescence-based biosensor has been developed for the selective and sensitive detection of Pb2+ ions using a DNAzyme system that responds only to the presence of lead (II) ions (Pb2+). This novel biosensing approach offers insights into the rapid and sensitive detection of major pollutants for food and environmental safety.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Chemistry, Analytical
Xiaoyan Wang, Siyu Xiao, Changping Yang, Congyi Hu, Xue Wang, Shujun Zhen, Chengzhi Huang, Yuanfang Li
Summary: A novel ratiometric electro-chemiluminescence biosensor utilizing zinc-metal organic frameworks (Zn-MOFs) as a single luminophore has been developed for ultrasensitive detection of miRNA-133a. The biosensor, which does not require an exogenous coreactant, demonstrated excellent accuracy and stability. This study provides a new perspective on fabricating a ratiometric ECL biosensor platform using Zn-MOFs as a single emitter.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Chen Chen, Jin-Xin Ma, Hui Wang, Hui-Qiao Liu, Shu-Wei Ren, Jun-Tao Cao, Yan-Ming Liu
Summary: A spatially resolved ratiometric electrochemiluminescence immunosensor was developed for myoglobin detection, using resonance energy transfer for signal amplification. The sensor exhibited high selectivity, sensitivity, and accuracy, making it a promising tool for biomarker analysis.
NEW JOURNAL OF CHEMISTRY
(2022)
Article
Spectroscopy
Weikang Yang, Xinyu Zheng, Feng Gao, Huihui Li, Bo Fu, Dong-Yu Guo, Fuxiang Wang, Qinhe Pan
Summary: A CdTe QDs@ZIF-8 composite was prepared and applied for the detection of CTC residue in animal food products. The composite exhibited a short response time and a low detection limit, and showed excellent recyclability. Visual determination of CTC was achieved through the manufacture of portable test strips.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Chemistry, Analytical
Zhiwen Gan, Tian Zhang, Yongmei Hu, Shujun Zhen, Xiaoli Hu
Summary: Developing ratiometric fluorescence probes for biothiols is of great significance. A simple ratiometric sensor for biothiols was developed based on CdTe quantum dots, which exhibited enhanced fluorescence and quenched second-order scattering in the presence of biothiols. The sensor showed fast response, simplicity, high sensitivity, and strong specificity, making it suitable for the quantification of biothiols in real samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Keqin Yang, Li Hou, Zhifang Li, Tianran Lin, Jianniao Tian, Shulin Zhao
Summary: A ratiometric fluorescent nanoprobe for detecting peroxynitrite was successfully constructed, showing selective accumulation in mitochondria and linear detection in the range of 0-6.0 µM. This nanoprobe enables ratiometric fluorescence imaging of endogenous peroxynitrite in cell mitochondria.
Article
Chemistry, Analytical
Mengmeng Guo, Na Luo, Yueling Bai, Zhenggang Xue, Qingmin Hu, Jiaqiang Xu
Summary: A porous heterostructure WO3-C/In2O3 was designed and prepared for a miniature H2 sensor, which showed higher response value, lower operating temperature, fast response-recovery speed, and low limit of detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Feng Hu, Hui Hu, Yuting Li, Xiaohui Wang, Xiaowen Shi
Summary: Arsenic contamination in water bodies is a significant health risk. This study developed a chitosan-catechol modified electrode for rapid and accurate detection of trace amounts of arsenic. The modified electrode demonstrated good detection capability and resistance to ionic interference, making it suitable for in situ detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Yantao Zhang, Qian Liu, Tao Tian, Chunhua Xu, Pengli Yang, Lianju Ma, Yi Hou, Hui Zhou, Yongjun Gan
Summary: In this study, a lysosome-targeting buffering fluorogenic probe (Lyso-BFP) was designed and synthesized, demonstrating excellent photostability, pH specificity, and responsiveness to lysosomal acidification in living cells. The performance of Lyso-BFP in pH sensing was attributed to the inhibition of the photo-induced electron transfer process. Lyso-BFP allowed for wash-free imaging and long-term real-time monitoring of lysosome pH changes based on its off-on fluorescence behavior and buffer strategy.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Wei Cai, Wenbo Sun, Jiayue Wang, Xiaokui Huo, Xudong Cao, Xiangge Tian, Xiaochi Ma, Lei Feng
Summary: In this study, a near-infrared fluorescent probe HCBG was developed for imaging of alpha-GLC. HCBG exhibited excellent selectivity and sensitivity towards alpha-GLC in complex bio-samples, and showed good cell permeability for in situ real-time imaging. Through the high-throughput screening system established by HCBG, a natural alpha-GLC inhibitor was successfully isolated and identified. This study provides a novel fluorescence visualization tool for discovering and exploring the biological functions of diabetes-related gut microbiota, and a high-throughput screening approach for alpha-GLC inhibitor.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Trey W. Pittman, Xi Zhang, Chamindie Punyadeera, Charles S. Henry
Summary: Heart failure is a growing epidemic and a significant clinical and public health problem. Researchers have developed a portable and affordable diagnostic device for heart failure that can be used at the point-of-care, providing a valid alternative to current diagnostics approaches.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Anders O. Tjell, Barbara Jud, Roland Schaller-Ammann, Torsten Mayr
Summary: An optical hydrogen peroxide sensor based on catalytic degradation and the detection of produced oxygen is presented. The sensor offers higher resolution and better sensitivity at lower H2O2 concentrations. By removing O2 from the sample solution, a more sensitive O2 sensor can be used for measurement. The sensor has been successfully applied in a flow-through cell to measure H2O2 concentration in different flow rates.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Seong Jae Kim, Ji-hun Jeong, Gaabhin Ryu, Yoon Sick Eom, Sanha Kim
Summary: Surface-enhanced Raman spectroscopy (SERS) is a high-sensitivity, label-free detection method with various analytical applications. Researchers have developed a hydrophobic SERS substrate based on engineered carbon nanotube arrays (CNT-SERS) and studied the role of structural design at both micro and nanoscales. The substrate demonstrated controlled self-enrichment capability and enhanced sensitivity, with a significant increase in the SERS signal. The study also proposed a theoretical model and a concentration strategy inspired by plants for analyte deposition on microarrays.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Dan Zhao, Renjun Jiang, Xiaoqiang Liu, Subbiah Alwarappan
Summary: In this study, a novel ternary composite material was constructed by assembling cerium vanadate nanorods on reduced graphene oxide-microcrystalline cellulose nanosheets, and it was used for real-time monitoring of the concentration of superoxide anions in vivo. The ternary composite showed excellent conductivity, large surface area, and abundant active sites, leading to a wider linear range, high sensitivity, low detection limit, and fast response time for superoxide anion detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Tengfei Wang, Liwen Wang, Guang Wu, Dating Tian
Summary: In this study, a covalent organic framework material TaTp-COF with porous and uniform spheres was successfully prepared via hydrothermal reaction, and it was found to significantly enhance the aggregation-induced emission (AIE) of berberine. The unique emission properties of berberine on TaTp-COF were studied and utilized for the sensitive detection of berberine.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Lin Li, Yilei Ding, Lei Xu, Shuoran Chen, Guoliang Dai, Pengju Han, Lixin Lu, Changqing Ye, Yanlin Song
Summary: In this study, a novel TTI based on a ratiometric fluorescent nanosensor is designed, which has the advantages of high accuracy and low cost. Experimental and theoretical investigations confirm its pH responsiveness and demonstrate its good sensitivity and reliability. By monitoring the total volatile basic nitrogen, this TTI can accurately predict food spoilage and can be adaptively modified for different types of food. The TTI based on this nanosensor enables visual monitoring of food quality.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Fangju Chen, Xueting Wang, Wei Chen, Chenwen Shao, Yong Qian
Summary: Lung cancer is the second most common malignant tumor worldwide. Drug resistance in lung cancer leads to treatment failure and recurrence in majority of patients. This study developed a fluorescent prodrug that can be activated in cancer cells to release drugs, and its signal can be tracked by imaging. It shows a unique autophagy-driven ferroptosis effect, indicating its potential for targeting drug-resistant cancer cells.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Weichao Li, Qiming Yuan, Zhangcheng Xia, Xiaoxue Ma, Lifang He, Ling Jin, Xiangfeng Chu, Kui Zhang
Summary: This study developed a high-performance gas sensor for formaldehyde detection by modifying ZnSnO3 with ZnO QDs and SnO2 QDs. The modified sensor showed improved sensing response and lower working temperature. The presence of ZnO QDs formed rich heterojunctions, increased surface area, and provided oxygen deficiency for formaldehyde sensing reaction, thus enhancing the sensor performance. This research provides an alternative method to enhance the sensing properties of MOS by QDs modification.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Joung-Il Moon, Eun Jung Choi, Younju Joung, Jin-Woo Oh, Sang-Woo Joo, Jaebum Choo
Summary: A novel nanoplasmonic substrate was developed for biomedical applications, which showed strong hot spots for detecting biomarkers at low concentrations. The substrate, called AuNPs@M13, was made by immobilizing 60 nm gold nanoparticles onto the surface of an M13 bacteriophage scaffold. It demonstrated higher sensitivity and lower limit of detection compared to commercially available assays.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Ning Li, Ya Zhang, Ying Xu, Xiaofang Liu, Jian Chen, Mei Yang, Changjun Hou, Danqun Huo
Summary: The molecular subtype of breast cancer guides treatment and drug selection. Invasive tests can promote cancer cell metastasis, so the development of high-performance, low-cost diagnostic tools for cancer prognosis is crucial. Liquid biopsy techniques enable noninvasive, real-time, dynamic, multicomponent, quantitative, and long-term observations at the cellular, genetic, and molecular levels. A Cu-Zr metal-organic framework (MOF) nanoenzyme with monatomic Cu attachment has been synthesized and proven to have high catalytic performance. The sensor constructed using this nanoenzyme shows potential for accurate classification of breast cancer serum samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Jeongmin Kim, Hyemin Kim, Seunghyun Park, Hyeonaug Hong, Yong Jae Kim, Jiyong Lee, Jaeho Kim, Seung-Woo Cho, Wonhyoung Ryu
Summary: This study presents a method to fabricate independently functioning microneedle (MN) electrodes with narrow intervals for high precision electrochemical sensing. The optimized mixture of photocurable polymer and single-wall carbon nanotubes was used to mold single composite MNs, which were then attached to pre-patterned electrodes. Plasma etching and electropolymerization were performed to enhance the electrochemical activity, and Prussian blue and glucose oxidase were electrodeposited on the MNs for glucose detection. The MN electrodes showed good sensitivity and linearity, and the feasibility of glucose detection was demonstrated in an in vivo mouse study.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)