期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 243, 期 -, 页码 484-488出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2016.12.008
关键词
Lateral flow assays; Hook effect; Semi-quantitative; Point-of-care testing
资金
- International Science & Technology Cooperation Program of China [2013DFG02930]
- National Instrumentation Program [2013YQ190467]
- Fundamental Research Funds for the Central Universities
- National Key Research and Development Program [2016YFC1101302]
- Ministry of Higher Education (MOHE), Government of Malaysia [UM.C/HIR/MOHE/ENG/44]
Lateral flow assays (LFAs) have found applications in clinical diagnostics, veterinary medicine, food safety, environmental monitoring, bio-defence, drug abuse, etc. However, conventional LFAs with single test zone for one analyte (sLFA) show qualitative, positive/negative results (that are usually not so accurate for decision-making) and suffer from the hook effect (that easily induces false negative results). In this study, a modified LFA with multiple test zones for one analyte (mLFA) is developed for detection of nucleic acid showing two methods to interpret assay results. One is the sum of detection signals of test zones, which exhibits a stable and broader detection range compared with sLFA. The other is the pattern of test zones that is presented is correlated to the concentration of analyte, facilitating semi-quantitative detection without the aid of a reader or analyzer. (C) 2016 Elsevier B.V. All rights reserved.
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