期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 246, 期 -, 页码 271-277出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2017.02.067
关键词
Gold nanoparticle; Silver enhancement; Signal amplification; Self-assembly; Enzyme activity
资金
- National Research Foundation (NRF) grant - Korean government (MSIP) [2016R1A2B2011744]
- Basic Science Research Program through the NRF - Ministry of Education, Korea [2012R1A6A1029029]
- National Research Foundation of Korea [2016R1A2B2011744] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Despite the simplicity and usability of gold nanoparticle (AuNP)-based colorimetry in a large panel of bioassays, this technique still suffers from a poor detection limit. To overcome this issue, we report an improved AuNP-based colorimetric assay in combination with simple centrifugation and silver enhancement. As a proof-of-concept, two types of enzymes (i.e., protein phosphatase and protease) were constructed based on the self-assembly of thiol-stabilized AuNPs in the presence of peptides and metal ions. When the AuNP solutions were subjected to a short cycle of centrifugation and silver enhancement, the signal-to-background ratio (SBR) was distinctly improved by a factor of 5-10 in both enzyme activity assays, as compared to that of AuNP-based colorimetry. The detection limit achieved by using the silver enhancement was determined to be improved by a factor of 1.0-3.4. Furthermore, grayscale images of the silver enhancement allowed for a rapid andsimple enzyme assay that did not require measuring.the absorbance. Due to its ability to improve the detection senstivity in a facile way, we anticipate that this approach will be suitable for use in many AuNP colorimetric assays. (C) 2017 Elsevier B.V. All rights reserved.
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