Article
Microbiology
Owen Higgins, Alexandra Chueiri, Louise O'Connor, Sinead Lahiff, Liam Burke, Dearbhaile Morris, Nicola Maria Pfeifer, Belen Gonzalez Santamarina, Christian Berens, Christian Menge, Manuela Canica, Vera Manageiro, Veljo Kisand, Marwa M. M. Hassan, Brian Gardner, Arnoud H. M. van Vliet, Roberto M. La Ragione, Bruno Gonzalez-Zorn, Terry J. J. Smith
Summary: CTX-M ESBL-producing E. coli is an increasing public health concern, and accurate identification of CTX-M variants is crucial for disease monitoring and treatment. CTX-M-1 and CTX-M-15 are the main ESBL-producing Enterobacteriaceae associated with animal and human infections.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Lorena Lopez-Cerero, Emilio Stolz, Marina R. Pulido, Alvaro Pascual
Summary: This study reports the first occurrence of community cases of CTX-M-27-producing Shigella sonnei ST152 in Spain. These cases were resistant to quinolones and azithromycin and included both adult males and a pediatric case. The isolates were genetically similar to an Australian isolate and differed significantly from outbreak-causing strains in England.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Biochemistry & Molecular Biology
Satoshi Nishida, Masataka Nakagawa, Yuki Ouchi, Chiaki Sakuma, Yu Nakajima, Hisayo Shimizu, Takashi Shibata, Yasunori Kurosawa, Toshiaki Maruyama, C. J. Okumura, Nami Hatayama, Yoshinori Sato, Miwa Asahara, Shinobu Ishigaki, Taiji Furukawa, Teruo Akuta, Yasuo Ono
Summary: An LFIA based on an anti-CTX-M rabbit monoclonal antibody was developed for detecting CTX-M enzyme production in Enterobacterales, showing high sensitivity and specificity with clinical isolates. This LFIA has a low detection limit, 100% sensitivity and specificity with clinical isolates, and does not cross-react with other class A beta-lactamase producing bacteria.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Infectious Diseases
Mohamed Belmahdi, Nadia Safia Chenouf, Abdelkrim Ait Belkacem, Sandra Martinez-Alvarez, Mario Sergio Pino-Hurtado, Zahra Benkhechiba, Samiha Lahrech, Ahcene Hakem, Carmen Torres
Summary: This study investigates the antimicrobial resistance and virulence threats of Escherichia coli isolates recovered from intestinal and fecal samples of chickens, turkeys, and sparrows. The study finds that sparrows could be a vector in the dissemination of ESBL-producing E. coli isolates to other environments. Additionally, this study reports the first detection of the bla(CTX-M-14) gene from sparrows at a global level and in turkeys in Algeria.
Article
Microbiology
Linda Falgenhauer, Anja Zur Nieden, Susanne Harpel, Jane Falgenhauer, Eugen Domann
Summary: The presence of ESBL-producing E. coli in surface water, closely related to human clinical isolates, suggests a potential transmission pathway. This highlights the underreported role of surface water in the spread of ESBL-producing isolates.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Fisheries
S. Ranjani, Pradeep Parthasarathy, P. Rameshkumar, U. VimalKumar, S. Hemalatha
Summary: The study successfully controlled the proliferation of bacterial pathogens causing vibriosis by synthesizing pungent acrid silver nanocolloids, which are cost-effective and can be used as an alternative to prevent disease outbreaks.
AQUACULTURE INTERNATIONAL
(2021)
Article
Infectious Diseases
Camila Pereira Silva, Celso Jose Bruno de Oliveira, Elma Lima Leite, Samuel Paulo Cibulski, Magda Fernandes, Priscylla Carvalho Vasconcelos, Larissa Maranhao Dias, Nubia Michelle Vieira da Silva, Felicio Garino Junior, Artur Cesar de Carvalho Fernandes
Summary: This study investigated the genetic context of expanded-spectrum 13-lactam resistance in a Klebsiella pneumoniae strain causing a hard-to-treat nasal infection in a domestic cat. The strain was found to carry multiple genes conferring resistance to various antimicrobial drugs. The results highlight the importance of horizontal gene transfer in the acquisition of antimicrobial resistance genes and emphasize the need for a One Health approach to understand the emergence and dissemination of antimicrobial resistance.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2022)
Article
Infectious Diseases
Elizabeth Liedhegner, Brandon Bojar, Rachelle E. Beattie, Caitlin Cahak, Krassimira R. Hristova, Troy Skwor
Summary: The study highlights the similarity in antibiotic resistance patterns and pathogenic potential between clinical strains and treated effluent E. coli populations, indicating that effluents could serve as a reservoir for future multi-drug resistant E. coli clinical infections.
Article
Infectious Diseases
Alice Elena Ghenea, Ovidiu Mircea Zlatian, Oana Mariana Cristea, Anca Ungureanu, Radu Razvan Mititelu, Andrei Theodor Balasoiu, Corina Maria Vasile, Alex-Ioan Salan, Daniel Iliuta, Mihaela Popescu, Anca-Loredana Udristoiu, Maria Balasoiu
Summary: CTX-M β-lactamases have become the most prevalent ESBLs in Enterobacteriaceae. This study identified the prevalence of bla(CTX-M-15), bla(SHV-1), and bla(TEM-1) encoding β-lactamases in Escherichia coli (E. coli) and Klebsiella pneumoniae (K. pneumoniae) strains in a Romanian hospital's ICU. The findings highlight the importance of reducing antibiotic use and improving resistance surveillance to guide local antibiotic therapy.
Article
Veterinary Sciences
Kayode Fashae, Ines Engelmann, Stefan Monecke, Sascha D. Braun, Ralf Ehricht
Summary: The study in Ibadan, Nigeria revealed the presence of ESBL-producing E. coli in both wild birds and slaughtered cattle, indicating a potential for inter-species transmission and virulence. Guidelines balancing public health and habitat conservation are recommended, with continuous surveillance needed.
BMC VETERINARY RESEARCH
(2021)
Article
Microbiology
Lorena Montero, Jorge Irazabal, Paul Cardenas, Jay P. Graham, Gabriel Trueba
Summary: The majority of untreated wastewater, containing drug-resistant and pathogenic bacteria, is released into streams in cities around the world, potentially leading to the circulation of antibiotic-resistant bacteria back to humans through agricultural produce. This study conducted in Ecuador found ESBL-producing E. coli in irrigation water, vegetables, and fruits, with certain ESBL genes associated with human infections being prevalent. The presence of mcr-1 gene responsible for colistin resistance in some isolates highlights the potential role of irrigation water in the antimicrobial resistance crisis in Ecuador.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Andrew Cameron, Rupinder Mangat, Heba H. Mostafa, Samantha Taffner, Jun Wang, Ghinwa Dumyati, Richard A. Stanton, Jonathan B. Daniels, Davina Campbell, Joseph D. Lutgring, Nicole D. Pecora
Summary: Infections caused by ESBL-producing Escherichia coli are a significant health issue globally, with ST131 being the predominant clonal type. Besides bla(CTX-M-15), other ESBL variants may also be prevalent in certain regions. The identification of ST38 ESBL-producing E. coli in different regions of the United States, carrying bla(CTX-M-27) on distinct plasmid types, suggests the potential emergence of new ESBL lineages.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Microbiology
Cecile Emeraud, Samy Figueiredo, Remy A. Bonnin, Mouna Khecharem, Souad Ouzani, Pierre-Etienne Leblanc, Agnes B. Jousset, Nicolas Fortineau, Jacques Duranteau, Laurent Dortet
Summary: Infections caused by ESBL-KP are on the rise globally and often lead to outbreaks in ICUs. Despite enhanced hygiene measures and prevention controls during the COVID-19 pandemic, an ESBL-producing K. pneumoniae isolate still caused a nosocomial outbreak in an ICU dedicated to COVID-19 patients.
Article
Infectious Diseases
Daiga Galina, Andris Balins, Anda Valdovska
Summary: This study aimed to determine the prevalence of fecal ESBL-producing E. coli in pigs on large and small farms in Latvia, with a focus on beta-lactamase genes and antimicrobial resistance profiles. The findings showed a higher prevalence of ESBL-producing E. coli on large farms, with a variety of ESBL genes distributed, predominantly bla(TEM) and bla(CTX-M).
Article
Veterinary Sciences
Hayyun Durrotul Faridah, Freshindy Marissa Wibisono, Freshinta Jellia Wibisono, Nabilatun Nisa, Fatimah Fatimah, Mustofa Helmi Effendi, Emmanuel Nnabuike Ugbo, Aswin Rafif Khairullah, Shendy Canadya Kurniawan, Otto Sahat Martua Silaen
Summary: This study aimed to determine the prevalence of ESBL-producing E. coli in broiler chickens in Indonesia. The results showed that 86.1% of the isolated E. coli were resistant to beta-lactam antibiotics, with 94.1% carrying the bla(CTX-M) gene and 38.2% carrying the bla(TEM) gene. The findings suggest that broiler chickens may serve as a potential reservoir of ESBL-producing E. coli, posing a threat to human health. Public health education is recommended to mitigate this potential risk.
JOURNAL OF VETERINARY RESEARCH
(2023)
Article
Environmental Sciences
Marija Kvesic, Hrvoje Kalinic, Mia Dzelalija, Ivica Samanic, Roko Andricevic, Ana Maravic
Summary: The study investigated the impact of partially treated submarine effluents from wastewater treatment plants on the water column, revealing that differences in discharge areas may be due to the origin of treated wastewaters and environmental conditions. Analysis showed abundant antibiotic resistance genes in the microbiomes, with pathogenic genera introduced by effluents predicted to be major sources of multidrug resistance.
ENVIRONMENTAL POLLUTION
(2022)
Article
Engineering, Biomedical
Tomislav Roncevic, Marco Gerdol, Mario Mardirossian, Matko Males, Svjetlana Cvjetan, Monica Benincasa, Ana Maravic, Goran Gajski, Lucija Krce, Ivica Aviani, Jerko Hrabar, Zeljka Trumbic, Maik Derks, Alberto Pallavicini, Markus Weingarth, Larisa Zoranic, Alessandro Tossi, Ivona Mladineo
Summary: This study reveals the important role of antimicrobial peptides in the survival of the marine parasite Anisakis and elucidates their mechanism of action against bacterial infections. These peptides exhibit potent bactericidal activity against Gram-negative bacteria, including multi-drug resistant strains, making them promising candidates for further drug development.
ACTA BIOMATERIALIA
(2022)
Article
Microbiology
Marija Kvesic, Ivica Samanic, Anita Novak, Zeljana Fredotovic, Mia Dzelalija, Juraj Kamenjarin, Ivana Goic Barisic, Marija Tonkic, Ana Maravic
Summary: This study investigated the emergence of carbapenemase-producing Enterobacteriaceae in coastal waters of the central Adriatic Sea in Croatia, revealing the presence of extensively drug resistant and colistin-resistant isolates. Whole genome sequencing identified a variety of resistance and virulence genes, as well as the co-occurrence of carbapenemase genes bla(KPC-2) and bla(OXA-48) in certain strains, raising concerns about the spread of these infectious threats in the marine environment.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biophysics
Luka Gujinovic, Ana Maravic, Hrvoje Kalinic, Mia Dzelalija, Stefanija Sestanovic, Drazen Zanchi, Ivica Samanic
Summary: Marine biofilms frequently and spontaneously form on almost any submerged solid surface in seawater. This study investigated the composition and predicted functional potential of bacterial communities in surface biofilms and confirmed Vibrio gigantis as an early and abundant colonizer, functionally linked to genes associated with cell motility, surface attachment, and quorum sensing. The study also monitored the adhesion dynamics of V. gigantis on a solid surface and found a convincing tendency of spontaneous adhering.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2022)
Article
Environmental Sciences
Mia Dzelalija, Marija Kvesic, Anita Novak, Zeljana Fredotovic, Hrvoje Kalinic, Ivica Samanic, Marin Ordulj, Slaven Jozic, Ivana Goic Barisic, Marija Tonkic, Ana Maravic
Summary: This study investigated the antibiotic resistance and virulence genes, as well as the sequence types (STs) affiliation, of vancomycin-resistant Enterococcus faecium (VREfm) recovered from marine beach waters, submarine outfall of a wastewater treatment plant, and offshore discharge of untreated sewage. The VREfm recovered from wastewater, coastal bathing waters, and the hospital showed similar virulence, multidrug resistance, and ST profiles, indicating a major public health threat.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Environmental Sciences
Ana Puljko, Svjetlana Dekic Rozman, Ivan Barisic, Ana Maravic, Marko Jelic, Ivana Babic, Milena Milakovic, Ines Petric, Nikolina Udikovic-Kolic
Summary: The study aimed to quantify enteric opportunistic pathogens (EOPs), resistance genes, and isolate enterobacterial strains from hospital wastewater. The results revealed high levels of ESBL and carbapenemase-producing Enterobacterales (CPE) isolates, all of which were multidrug-resistant. Therefore, effective pretreatment of hospital wastewater is necessary to prevent the transmission of clinically important pathogens and resistance genes.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Multidisciplinary Sciences
Danijela Santic, Iva Stojan, Frano Matic, Zeljka Trumbic, Ana Vrdoljak Tomas, Zeljana Fredotovic, Kasia Piwosz, Ivana Lepen Pleic, Stefanija Sestanovic, Mladen Solic
Summary: By combining qualitative 16S metabarcoding and quantitative CARD-FISH methods with neural gas analysis, this study revealed different patterns of the picoplankton community in response to changing environmental conditions in the Adriatic Sea. The study shows that the initial state of community structure changes according to environmental conditions and is expressed as qualitative and quantitative changes. A general pattern of increasing diversity and decreasing abundance of the community was observed, especially under harsh environmental conditions and increasing salinity.
SCIENTIFIC REPORTS
(2023)
Article
Horticulture
Marija Nazlic, Dario Kremer, Zeljana Fredotovic, Zeljka Trumbic, Valerija Dunkic, Jasna Puizina
Summary: The purpose of this study was to compare the free volatile compounds of 18 Veronica species with their DNA sequences. Two sets of DNA sequence data were generated and used for phylogenetic analysis. The results showed that the Veronica species were grouped into eight subgenera, and microwave-extracted volatiles showed better clustering in the analysis of free volatile compounds.
Article
Engineering, Environmental
Mia Dzelalija, Marija Kvesic-Ivankovic, Slaven Jozic, Marin Ordulj, Hrvoje Kalinic, Antonio Pavlinovic, Ivica Samanic, Ana Maravic
Summary: This study, for the first time, investigates antibiotic resistance in marine environments, finding that seasonal and spatial variations have an impact on microbial communities and resistomes in the ocean, depending on the trophic gradient. Pathogenic genera play a significant role as carriers of multiple resistance traits, and the diversity of resistance genes varies across different seasons and antibiotics. These findings highlight the importance of understanding the distribution and diversity of antibiotic resistance in marine ecosystems for environmental protection and public health.
Article
Agronomy
Marija Nazlic, Valerija Dunkic, Mia Dzelalija, Ana Maravic, Mihaela Mandic, Sinisa Srecec, Ivana Vrca, Elma Vuko, Dario Kremer
Summary: Agricultural production without pollution can be achieved by using bioactive plant compounds, such as essential oils. This study evaluated the antiphytoviral and antibacterial potentials of extracts from five Veronica species using different extraction methods. Some extracts showed effective inhibition of tobacco mosaic virus and demonstrated significant antibacterial activity. Valuable bioproducts can be obtained from these Veronica species for safe use in agriculture and food preservation.
Article
Biochemistry & Molecular Biology
Ivica Samanic, Blanka Dadic, Zeljka Sanader Marsic, Mia Dzelalija, Ana Maravic, Hrvoje Kalinic, Pavle Vrebalov Cindro, Zeljko Sundov, Marija Tonkic, Ante Tonkic, Jonatan Vukovic
Summary: This study investigated the molecular genetic mechanisms of clarithromycin and levofloxacin resistance in Helicobacter pylori infections in patients from Croatia. Point mutations in the 23S rRNA, gyrA, and gyrB genes were found to alter target sites or protein structure, leading to reduced efficacy of standard antibiotics. Molecular docking analysis revealed that resistance-related mutations resulted in decreased susceptibility to clarithromycin and levofloxacin due to significant differences in non-covalent interactions, ultimately resulting in antibiotic resistance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Ivana Vrca, Dina Ramic, Zeljana Fredotovic, Sonja Smole Mozina, Ivica Blazevic, Tea Bilusic
Summary: This study investigates the effect of two modern extraction methods on the volatile components of garden nasturtium seeds. The results show that the essential oil of garden nasturtium seeds exhibits stronger antiproliferative and antimicrobial activity compared to the plant extract.
FOOD TECHNOLOGY AND BIOTECHNOLOGY
(2022)
Article
Engineering, Environmental
Ana Puljko, Milena Milakovic, Stela Krizanovic, Josipa Kosic-Vuksic, Ivana Babic, Ines Petric, Ana Maravic, Marko Jelic, Nikolina Udikovic-Kolic
Summary: Extended-spectrum beta-lactamase (ESBL) and carbapenemase-producing Enterobacterales are still present in the environment even after conventional treatment in wastewater treatment plants. Enteric opportunistic pathogens such as Klebsiella pneumoniae and Acinetobacter baumannii are efficiently removed, but Escherichia coli and Enterococcus spp. still remain in relatively high concentrations in the effluent. Improvement in wastewater treatment technologies is necessary to minimize the risk of environmental contamination and public health issues related to ESBL and carbapenemase-producing Enterobacterales.
JOURNAL OF HAZARDOUS MATERIALS
(2022)