Article
Dermatology
Shu-Hao Chang, Pei-Ying Lin, Tsai-Kun Wu, Chien-Sheng Hsu, Shi-Wei Huang, Zheng-Yi Li, Kuang-Ting Liu, Jun-Kai Kao, Yi-Ju Chen, Tak-Wah Wong, Chun-Ying Wu, Jeng-Jer Shieh
Summary: Our experiments demonstrated that IMQ not only caused lysosome damage and dysfunction in skin cancer cells, but also activated caspase-8 through ROS accumulation, leading to lysosomal cell death. Inhibition of lysosomal proteases and treatment with lysosome-alkalizing agents enhanced the cytotoxicity of IMQ in vitro and in vivo, highlighting the potential role of the CTSD/caspase-8 axis in IMQ-induced cell death.
JOURNAL OF DERMATOLOGICAL SCIENCE
(2022)
Review
Neurosciences
Gretchen A. Johnson, Raghu R. Krishnamoorthy, Dorota L. Stankowska
Summary: Efficient cellular communication is crucial for brain functions such as muscle contractions, memory formation, decision-making, and task execution. Calcium signaling is related to mitochondria, and mitochondrial dysfunction may play a significant role in multiple neurodegenerative diseases and aging.
FRONTIERS IN NEUROSCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Debasna Pritimanjari Panigrahi, Srimanta Patra, Bishnu Prasad Behera, Pradyota Kumar Behera, Shankargouda Patil, Birija Sankar Patro, Laxmidhar Rout, Itisam Sarangi, Sujit Kumar Bhutia
Summary: Stress-induced mitochondrial hyperfusion (SIMH) has a protective effect on cell survival, but in the absence of mitochondrial fission, SIMH can induce apoptosis related to oxidative stress. This study found that MTP18, a protein promoting mitochondrial fission, is increased in oral cancer. A novel inhibitor of MTP18 called S28 was identified, which induces SIMH and triggers apoptosis. S28 inhibits MTP18-mediated mitochondrial fission and induces autophagy, resulting in the loss of mitochondrial membrane potential and the generation of mitochondrial superoxide to induce apoptosis. Combining S28 with FDA-approved anticancer drugs enhances apoptotic activity and decreases cell viability.
FREE RADICAL BIOLOGY AND MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Yu-Jeong Choi, Youn Kyung Choi, Seong-Gyu Ko, Chunhoo Cheon, Tai Young Kim
Summary: Costunolide (CTL) induces apoptosis by increasing reactive oxygen species (ROS) generation in cancer cells. It has a stronger cytotoxic effect on SK-BR-3 cells compared to MCF-7 cells. This difference is due to the activation of mitochondrial-dependent intrinsic apoptosis pathway in SK-BR-3 cells and PINK1/Parkin-dependent mitophagy pathway in MCF-7 cells in response to CTL treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Environmental Sciences
Nengzhou Chen, Zhenkun Guo, Zhousong Luo, Fuli Zheng, Wenya Shao, Guangxia Yu, Ping Cai, Siying Wu, Huangyuan Li
Summary: Long-term exposure to paraquat may increase the risk of Parkinson's disease, with ROS-mediated mitochondrial fission contributing to excessive mitophagy, which can be significantly ameliorated by mdivi-1.
ENVIRONMENTAL POLLUTION
(2021)
Article
Multidisciplinary Sciences
Tianshu Hao, Jianglong Yu, Zhida Wu, Jie Jiang, Longlong Gong, Bingjun Wang, Hanze Guo, Huabin Zhao, Bin Lu, Simone Engelender, He He, Zhiyin Song
Summary: Mitochondria are key organelles for oxygen sensing, while lysosomes contain hydrolytic enzymes for degradation. This study shows that under hypoxia, mitochondria engulf and degrade lysosomes, leading to the formation of large mitochondria and a process called mitochondrial self-digestion. These findings uncover a mode of communication between mitochondria and lysosomes, as well as a novel pathway for mitochondrial degradation.
NATURE COMMUNICATIONS
(2023)
Article
Neurosciences
Yuliya Medvedeva, Hong Z. Yin, Afsheen Bazrafkan, Andriy Yeromin, Sung G. Ji, Eli J. Weiss-Hung, Edward Sharman, Alyssa P. Avilez, Niki Maki, Masih A. Rafi, Guilian Tian, Yama Akbari, John H. Weiss
Summary: Zn2+ accumulation and entry into mitochondria contribute to acute neuronal death following ischemia. Inhibition of Zn2+ entry, either with mitochondrial Ca2+ uniporter blockade or Zn2+ chelation, attenuates mitochondrial dysfunction and synaptic changes. Additionally, Zn2+ accumulation in damaged mitochondria after transient global ischemia suggests a potential target for neuroprotective therapy.
JOURNAL OF NEUROSCIENCE
(2022)
Article
Nutrition & Dietetics
Ciro Menale, Giovanna Trinchese, Immacolata Aiello, Giulia Scalia, Monica Dentice, Maria Pina Mollica, Nal Ae Yoon, Sabrina Diano
Summary: In this study, it was found that the addition of non-lipotoxic levels of 25 μM palmitic acid (PA) to glucose (G) increased mineralization in osteoblasts (OBs). It was also discovered that G+25 μM PA reduced mitochondria size in OBs and increased mitochondria respiration and ATP production, as well as the expression of oxidative phosphorylation genes. Treatment with the mitochondrial fission inhibitor Mdivi-1 reduced osteogenesis and mitochondrial respiration in OBs.
Article
Biochemistry & Molecular Biology
Parash Prasad, Sampurna Ghosh, Sib Sankar Roy
Summary: Glutamine deficiency in the core of tumors can increase the cancer stem cell population, and the combination therapy with MDiVi-1 and L-DON is an effective approach to reduce CSCs population in tumor. The study reveals the association between glutamine deprivation and stemness in cancer cells, highlighting the potential of targeting glutamine metabolism for cancer therapy. The findings suggest that metabolic reprogramming induced by glutamine starvation plays a key role in promoting the stem cell characteristics in tumor cells.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Cardiac & Cardiovascular Systems
Xiaoxiao Liu, Minghui Li, Zhiwei Chen, Yong Yu, Hui Shi, Ying Yu, Yucheng Wang, Ruizhen Chen, Junbo Ge
Summary: This study found that calpain plays an important role in Coxsackievirus-induced myocarditis and revealed its underlying mechanism of action. The NLRP3 inflammasome was also found to be involved in the process. These findings suggest that calpain may be a promising target for the treatment of viral myocarditis.
BASIC RESEARCH IN CARDIOLOGY
(2022)
Article
Cell Biology
Evan Downs, Amber D. Bottrell, Kari Naylor
Summary: Research has linked defects in mitochondrial dynamics and an increase in reactive oxygen species to the pathogenesis of neurodegenerative diseases. This study focused on examining the effects of ROS on mitochondrial dynamics in Dictyostelium discoideum, finding that there is no clear pattern in how different ROS generators affect mitochondrial dynamics and altering the microtubule cytoskeleton is not necessary to induce changes in response to ROS.
Article
Cell Biology
Qing-Rui Wu, Dan-Lin Zheng, Pei-Ming Liu, Hui Yang, Lu-An Li, Su-Juan Kuang, Ying-Yu Lai, Fang Rao, Yu-Mei Xue, Ji-Jin Lin, Shuang-Xin Liu, Chun-Bo Chen, Chun-Yu Deng
Summary: Mitochondrial dysfunction and impaired Ca2+ handling play crucial roles in the development of diabetic cardiomyopathy. Inhibiting Drp1 and Orai1 activity can alleviate cardiomyocyte hypertrophy and dysfunction induced by high glucose.
CELL DEATH & DISEASE
(2021)
Article
Pharmacology & Pharmacy
Ju Tang, Weixia Duan, Ping Deng, Huijuan Li, Cong Liu, Yu Duan, Min Feng, Shangcheng Xu
Summary: Cadmium exposure slows down the rapid movement of mitochondria in neurons, leading to abnormal distribution and excessive fragmentation of mitochondria, ultimately causing mitochondrial membrane potential loss and neuronal damage. Chelating overloaded cytosolic calcium is a promising strategy to prevent cadmium-induced neurotoxicity.
Article
Biochemistry & Molecular Biology
Xin Cai, Charles P. Ng, Olivia Jones, Tak Shun Fung, Keun Woo Ryu, Dayi Li, Craig B. Thompson
Summary: Lactate can enter the mitochondrial matrix and stimulate electron transport chain activity, increasing ATP synthesis and suppressing glycolysis. Unmetabolized D-lactate can reversibly inhibit oxidative phosphorylation and suppress glucose fermentation, promoting cell growth and function.
Article
Cell Biology
Dan He, Zhijie Ma, Ke Xue, Haiyan Li
Summary: JM2 inhibits breast tumor growth by inducing apoptosis and inhibiting proliferation, stimulating mitochondrial aggregation and regulating cell cycle. The JM2 grafted hyaluronic acid hydrogel effectively suppresses malignant proliferation of tumor cells and reduces breast tumor recurrence.
CELL DEATH & DISEASE
(2022)
Article
Biochemistry & Molecular Biology
Emily A. Caseley, Stephen P. Muench, Lin-Hua Jiang
Summary: The P2X7 receptor is a calcium-permeable cation channel activated by high concentrations of extracellular ATP, playing crucial roles in physiological and pathological processes. Investigation of the role of tyrosine at position 288 in the extracellular domain contributes to a deeper understanding of the structure-function relationships of the hP2X7R.
PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS
(2022)
Article
Cell Biology
Kenny Man, Habib Joukhdar, Xue D. Manz, Mathieu Y. Brunet, Lin-Hua Jiang, Jelena Rnjak-Kovacina, Xuebin B. Yang
Summary: Epigenetic reprogramming and silk scaffolds were used to promote bone formation of human dental pulp stromal cells (hDPSCs). The study demonstrated that MI192 pre-treatment significantly enhanced hDPSCs alkaline phosphatase activity and promoted bone extracellular matrix deposition and mineralisation. The hDPSCs-silk scaffold constructs also enhanced vascularisation and extracellular matrix mineralisation in vivo.
CELL AND TISSUE RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Xiaoling Jia, Chao Gao, Xia Wang, Lin-Hua Jiang, Yubo Fan
Summary: This study found that mechanical stretch had different effects on the expressions of BKCa and L-VDCC channels in rat uterine smooth muscle cells, with stretch down-regulating BKCa channel expression but upregulating L-VDCC channel expression.
JOURNAL OF MEMBRANE BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Stephanie Chadet, Jordan Allard, Lucie Brisson, Osbaldo Lopez-Charcas, Roxane Lemoine, Audrey Heraud, Stephanie Lerondel, Roseline Guibon, Gaelle Fromont, Alain Le Pape, Denis Angoulvant, Lin-Hua Jiang, Ruth Murrell-Lagnado, Sebastien Roger
Summary: The P2X4 purinergic receptor is upregulated in breast cancer patients and primarily localised in endolysosomes. It promotes breast cancer invasion, tumour growth, and metastasis by regulating lysosome acidity, promoting autophagy and cell survival. It is also associated with epithelial-to-mesenchymal transition and plays a more significant role under metabolic challenges.
Editorial Material
Physiology
Nady Braidy, Bilal Cig, Lin-Hua Jiang, Xinhua Shu
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Cell Biology
Xiaoling Jia, Xinlan Chen, Chao Gao, Haikun Wang, Chengxi Yang, Lin-Hua Jiang, Yubo Fan
Summary: The cooperation between IKCa and TRPC1 channels in mediating calcium influx and activating the ERK1/2 pathway is critical for vascular smooth muscle cell (VSMC) proliferation. Inhibition or knockdown of IKCa or TRPC1 suppresses serum-induced cell proliferation and increases in intracellular calcium concentration. Additionally, extracellular calcium removal or TRPC1 knockdown significantly inhibits VSMC proliferation. These findings provide new insights into the mechanisms underlying VSMC proliferation.
CELL PROLIFERATION
(2023)
Article
Neurosciences
Philippa Malko, Xiaoling Jia, Ian Wood, Lin-Hua Jiang
Summary: Microglial cell function and inflammation are regulated by the activation of Piezo1 channel. This study found that the activation of Piezo1 channel can inhibit the pro-inflammatory activation and production of pro-inflammatory cytokines in microglial cells by initiating intracellular Ca2+ signaling to inhibit the NF-kappa B inflammatory signaling pathway.
Review
Chemistry, Medicinal
Yaling Yin, Linyu Wei, Emily A. Caseley, Osbaldo Lopez-Charcas, Yingjuan Wei, Dongliang Li, Steve P. Muench, Sebastian Roger, Lu Wang, Lin-Hua Jiang
Summary: This review discusses the role and mechanisms of the P2X7 receptor in mediating neuroinflammation and other pathogenic events in the central nervous system, as well as its relationship with traumatic CNS damage and related complications. The article raises the perspective on the steady progress in developing CNS-penetrant P2X7 receptor-specific antagonists that leverage the ATP-P2X7 receptor signaling axis as a potential therapeutic strategy.
MEDICINAL RESEARCH REVIEWS
(2023)
Editorial Material
Cell Biology
Mo Zhang, Philippa Malko, Lin-Hua Jiang
NEURAL REGENERATION RESEARCH
(2023)
Article
Neurosciences
Han-Wei Liu, Li-Na Gong, Ke Lai, Xia-Fei Yu, Zhen-Qi Liu, Ming-Xian Li, Xin-Lu Yin, Min Liang, Hao-Song Shi, Lin-Hua Jiang, Wei Yang, Hai-Bo Shi, Lu-Yang Wang, Shan-Kai Yin
Summary: Stroke prognosis is negatively affected by elevated levels of serum bilirubin, but the mechanism of how bilirubin worsens outcomes is still unknown. This study found that post-stroke bilirubin levels, but not pre-stroke levels, are associated with infarct volume in hospitalized patients. Mouse models showed that bilirubin increases neuronal excitability and ischemic infarct size, and ischemic insults trigger the release of endogenous bilirubin, which activates TRPM2 channels. Knockout or antagonization of the TRPM2 channel attenuates these effects. These findings suggest a vicious cycle of stroke injury involving the release of endogenous bilirubin and activation of TRPM2 channels, exacerbating Ca2+-dependent brain injury.
Editorial Material
Cell Biology
Lin-Hua Jiang, Xiaoqiang Yao, Bilal cig
Article
Gastroenterology & Hepatology
Xiaobo Cai, Xiazhen Yu, Jiawen Yang, Lin Lu, Ning Hua, Xin Duan, Peiwu Ye, Lei Ni, Linhua Jiang, Wei Yang, Tingbo Liang, Peilin Yu
Summary: In this study, it was found that the TRPM2 channel plays a crucial role in the development and progression of HCC. Inhibition or silencing of TRPM2, as well as inhibition of the downstream signaling pathway, significantly suppressed the proliferation of HCC cells. Furthermore, inhibition or depletion of TRPM2 also slowed down the growth of HCC xenografts in mice, indicating its potential as a therapeutic target for HCC.
HEPATOLOGY COMMUNICATIONS
(2023)
Article
Neurosciences
Lu Wang, Yingjuan Wei, Zhenzhou Sun, Lin-Hua Jiang, Yaling Yin, Panpan Zheng, Yun Fu, Hongwei Wang, Changzheng Li, Jian-Zhi Wang
Summary: Tau protein hyperphosphorylation and NFTs formation are key features of AD, and DpdtpA can attenuate these processes by regulating the PI3K/AKT/GSK-36 signaling pathway. DpdtpA also reduces microglial activation and inflammatory responses induced by Tau, offering a potential treatment option for AD-associated neuroinflammation.
Article
Multidisciplinary Sciences
Cheng Zhong, Jing Yang, Yiyin Zhang, Xiaoxiao Fan, Yang Fan, Ning Hua, Duguang Li, Shengxi Jin, Yirun Li, Peng Chen, Yongle Chen, Xiaobo Cai, Yi Zhang, Linhua Jiang, Wei Yang, Peilin Yu, Hui Lin
Summary: This study found that TRPM2 deficiency could alleviate liver dysfunction, inflammation, and cell death caused by hepatic ischemia-reperfusion (IR) injury. Further research indicated that TRPM2 mediates hepatic IR injury through ferroptosis-related pathways. Animal experiments and in vitro studies showed that inhibition of TRPM2 and calcium depletion could significantly attenuate IR-induced ferroptosis. These findings reveal the crucial role of TRPM2-mediated ferroptosis in hepatic IR injury and suggest that pharmacological inhibition of TRPM2 may be an effective therapeutic strategy for hepatic IR injury-related diseases, such as during liver resection and transplantation.
Article
Engineering, Biomedical
Kenny Man, Cesar Alcala, Naveen V. Mekhileri, Khoon S. Lim, Lin-Hua Jiang, Tim B. F. Woodfield, Xuebin B. Yang
Summary: This study investigated a GelMA hydrogel reinforced with a 3D printed scaffold to support MI192-induced hBMSCs for bone formation. The results demonstrated that the GelMA-PEGT/PBT construct provides enhanced mechanical strength and facilitates the delivery of epigenetically-activated MSCs for bone augmentation strategies.
JOURNAL OF FUNCTIONAL BIOMATERIALS
(2022)