Article
Engineering, Environmental
Chien-Ping Wang, Chin-Sheng Chang, Wei-Chen Lin
Summary: This study investigated the effects of irradiance distribution, outer radius, radiative power, and flow rate on the inactivation efficiency of Escherichia coli using UV-C LEDs in a flow-through reactor. Results showed that using 24 LEDs yielded the highest inactivation efficiency, and increasing the outer radius increased exposure time but reduced average irradiance. Additionally, an increase in flow rate led to a sharp decrease in inactivation rate due to reduced exposure time.
JOURNAL OF WATER PROCESS ENGINEERING
(2021)
Article
Chemistry, Multidisciplinary
Xi Luo, Yifeng Wang, Weilong Zheng, Xiaolong Sun, Gaowei Hu, Longfei Yin, Yingying Zhang, Fengwei Yin, Yongqian Fu
Summary: Computer-aided rational design was used to improve the thermostability and catalytic activity of LrLDH, providing a reference for the modification of other enzymes.
Article
Chemistry, Analytical
Wen Liu, Linbo Wei, Dongmei Wang, Chengye Zhu, Yuting Huang, Zhengjun Gong, Changyu Tang, Meikun Fan
Summary: This study proposes a method to enhance the differentiation of bacterial SERS spectra by applying external environmental stress. The results show that appropriate stress can increase the differences in bacterial SERS spectra.
ANALYTICAL CHEMISTRY
(2022)
Article
Microbiology
Ethan R. Wyrsch, Kristina Nesporova, Hassan Tarabai, Ivana Jamborova, Ibrahim Bitar, Ivan Literak, Monika Dolejska, Steven P. Djordjevic
Summary: By conducting a comprehensive genomic study on Escherichia coli isolates from the Australian silver gull, this research reveals the presence and distribution of antibiotic resistance genes in different lineages of E. coli. The findings demonstrate the potential transmission of resistant and pathogenic E. coli between wildlife and human populations. The study highlights the importance of understanding the role of bystander species in the spread of antibiotic resistance.
Article
Biotechnology & Applied Microbiology
Yujia Jiang, Runze Pan, Yuxuan Tao, Wenlong Xiao, Wankui Jiang, Fengxue Xin, Wenming Zhang, Min Jiang
Summary: This study attempted to improve the environmental tolerance of E. coli for carboxylic acid production through biological membrane engineering. By introducing whiB and cti genes, the engineered E. coli achieved a higher SA production of 80.0 g/L in a 5-L fermenter, which was 37.0% higher than the control group. Further incorporation of fabA and fabB genes enhanced the strain's survival rate and metabolic performance under harsh environments, resulting in a SA production of 29.9 g/L with a yield of 0.79 g/g under low pH conditions in the fermenter. Membrane engineering proved to be an effective strategy in improving strain robustness and facilitating large-scale carboxylic acid production.
BIOCHEMICAL ENGINEERING JOURNAL
(2023)
Article
Engineering, Chemical
Maikon Eduardo dos Santos Soldan, Eduardo Borges Lied, Ismael Laurindo Costa, Paulo Rodrigo Stival Bittencourt, Ilton Jose Baraldi, Renata Mello Giona, Ana Paula Trevisan, Fernando Hermes Passig, Karina Querne de Carvalho
Summary: The present study investigates the adsorption behavior of ciprofloxacin hydrochloride (CIP) onto the cationic resin Amberlite IR120. The results suggest that IR120 holds promising potential as an adsorbent for CIP removal and can completely eliminate toxicity towards Escherichia coli organisms.
CHEMICAL ENGINEERING SCIENCE
(2023)
Article
Food Science & Technology
Federica Giacometti, Annalisa Pezzi, Giorgio Galletti, Marco Tamba, Giuseppe Merialdi, Silvia Piva, Andrea Serraino, Silva Rubini
Summary: The study investigated the antimicrobial resistance patterns of Salmonella and E. coli isolates from bivalve molluscs and water samples near Ferrara, Italy, finding high levels of resistance, with most resistant strains showing resistance to multiple antimicrobial agents.
Article
Infectious Diseases
Yitao Duan, Shuangqing Liu, Yuting Gao, Peng Zhang, Daqing Mao, Yi Luo
Summary: Macrolides can activate the transcription of the msr(E)-mph(E) operon through the involvement of HNS and CRP. The operon was found to exist widely in diverse species of bacteria, with Acinetobacter baumannii, Klebsiella pneumoniae, Escherichia coli, and Proteus mirabilis being the main carriers. These bacteria were primarily isolated from human clinical samples and had a wide geographical distribution.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2022)
Article
Food Science & Technology
Erton Gomes da Silva, Leonardo Ereno Tadielo, Thiago Henrique Belle, Emanoelli Aparecida Rodrigues dos Santos, Jhennifer Arruda Schmiedt, Fabio Sossai Possebon, Juliano Goncalves Pereira, Luciano dos Santos Bersot
Summary: This study found that reducing the water flow in the final wash of chicken slaughter process does not affect the microbiological quality of the chicken carcasses.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Immunology
Nadghia F. Leite-Sampaio, Cicera Natalia F. L. Gondim, Celestina E. Sobral de Souza, Henrique D. M. Coutinho
Summary: This study investigated the antibacterial and antimicrobial modulating activity of alpha-pinene and borneol against Escherichia coli and enteropathogenic and enterotoxigenic serotypes. The results showed that alpha-pinene and borneol exhibited low antimicrobial action against multidrug-resistant Escherichia coli, but no antimicrobial activity was observed against enteropathogenic and enterotoxigenic serotypes. A synergistic action of borneol with ciprofloxacin against enterotoxigenic serotypes was noted.
MICROBIAL PATHOGENESIS
(2022)
Article
Environmental Sciences
Yi Hong, Frederic Soulignac, Adelaide Roguet, Chenlu Li, Bruno J. Lemaire, Rodolfo Scarati Martins, Francoise Lucas, Brigitte Vincon-Leite
Summary: This study develops an integrated modeling approach to assess the impact of stormwater drainage on recreational water quality, demonstrating the potential for urban bathing water quality management through simulating the dynamics of Escherichia coli in urban catchments and lakes.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Chemistry, Physical
Balasubramani Ramprakash, Aran Incharoensakdi
Summary: Bacterial encapsulation is a novel biotechnological strategy for generating fuels and valuable chemicals. In this study, an optimal alginate encapsulated TiO2/Escherichia coli hybrid system was established to efficiently produce hydrogen under visible light. The encapsulated system exhibited enhanced hydrogen production even in the presence of oxygen.
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
(2022)
Article
Food Science & Technology
Haotian Deng, Jinyan Zhu, Yuqi Tong, Yanwen Kong, Chang Tan, Mingyue Wang, Meizhi Wan, Xianjun Meng
Summary: Aronia melanocarpa anthocyanins (AMAs) exhibit strong antibacterial activity against Escherichia coli by damaging the cell wall and membrane structure of the bacteria. By targeting multiple cellular components, AMAs have the potential to be used as natural food preservatives.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Biochemical Research Methods
Bor Kavcic, Gasper Tkacik, Tobias Bollenbach
Summary: Phenomenological relations like Ohm's or Fourier's laws, common in physics, have been less explored in biology, limiting predictive theory. This study builds a minimal biophysical model based on bacterial growth laws to predict antibiotic interactions. The model successfully accounts for different drug interactions, providing a mechanistic understanding of higher-order interactions and the impact of resistance genes. Experimentally verified, the model opens new possibilities for predicting drug interactions in diverse biological systems.
PLOS COMPUTATIONAL BIOLOGY
(2021)
Article
Biology
Gitanjali NandaKafle, Taylor Huegen, Sarah C. Potgieter, Emma Steenkamp, Stephanus N. Venter, Volker S. Brozel
Summary: Escherichia coli isolates from water, sediment, and aquatic plants in a peri-urban pond displayed similarities in phylogroup distribution, virulence gene distribution, and antibiotic resistance, while isolates from snails and cattle feces showed significant differences. Population structure analysis revealed three distinct genetic backgrounds associated with bovine, snail, and aquatic environments, supporting niche preference of E. coli isolates in the ecosystem studied.
Article
Public, Environmental & Occupational Health
Winnie Zambrana, Jingyan Tong, Claire E. Anderson, Alexandria B. Boehm, Marlene K. Wolfe
Summary: This study compared the efficacy of ash and sand as handwashing agents to commonly recommended methods. The results showed that using ash or sand can reduce viruses, but soap is more effective, especially when used for shorter durations.
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE
(2023)
Letter
Medicine, General & Internal
Marlene K. Wolfe, Alexander T. Yu, Dorothea Duong, Madhura S. Rane, Bridgette Hughes, Vikram Chan-Herur, Marisa Donnelly, Shua Chai, Bradley J. White, Duc J. Vugia, Alexandria B. Boehm
NEW ENGLAND JOURNAL OF MEDICINE
(2023)
Article
Engineering, Environmental
Lauren C. Kennedy, Veronica P. Costantini, Kimberly A. Huynh, Stephanie K. Loeb, Wiley C. Jennings, Sarah Lowry, Mia C. Mattioli, Jan Vinje, Alexandria B. Boehm
Summary: Human norovirus, a major cause of acute gastroenteritis, can be transmitted through water but its persistence in water is not well understood. Various factors such as genome damage and capsid cleavage can affect the loss of infectivity of human norovirus in surface water.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Review
Environmental Sciences
Sarah A. Lowry, Marlene K. Wolfe, Alexandria Boehm
Summary: Concentrations of nucleic acids from various respiratory viruses in wastewater solids collected from treatment plants can be correlated to clinical data on disease occurrence in the community. To establish this correlation, viral nucleic acid concentrations in human excretions need to be considered. In this study, a systematic review and meta-analysis were conducted to characterize the concentrations and presence of different respiratory viruses in various excretions. More concentration data, especially longitudinal data, are needed across all respiratory virus types and excretion types for a quantitative linking of virus wastewater concentrations to numbers of infected individuals.
JOURNAL OF WATER AND HEALTH
(2023)
Article
Multidisciplinary Sciences
Ryan T. T. Searcy, Jacob R. R. Phaneuf, Alexandria B. B. Boehm
Summary: Fecal indicator bacteria (FIB) are monitored to assess water quality and health risk at beaches, but infrequent monitoring can lead to inaccurate assessments. This study conducted high-frequency FIB sampling at an enclosed harbor in California, showing more variability in FIB concentrations compared to open beach sites. Correlation and regression analyses revealed strong associations between FIB concentrations and chlorophyll a, turbidity, wind speed, and tide level. The study emphasizes the importance of sampling FIB concentrations and environmental parameters at appropriate temporal resolutions for water quality monitoring.
Article
Multidisciplinary Sciences
Claire E. Anderson, Marlene K. Wolfe, Alexandria B. Boehm
Summary: This study aimed to test the virucidal effect of antimicrobial treatment on the Supertowel and to evaluate its viability as a handwashing alternative. The results showed that the Supertowel had similar viral inactivation as a regular microfiber towel, indicating minimal virucidal potential.
Article
Multidisciplinary Sciences
Sooyeol Kim, Alexandria B. Boehm
Summary: The study examines the effectiveness of using wastewater surveillance in schools to detect the presence of COVID-19 infections. The results show that infected individuals can be identified through wastewater testing, especially in solid samples which have a higher concentration of the virus compared to liquid samples.
Review
Multidisciplinary Sciences
Meghan M. Shea, Jacob Kuppermann, Megan P. Rogers, Dustin Summer Smith, Paul Edwards, Alexandria B. Boehm
Summary: The lack of universal guidelines in environmental DNA (eDNA) research has led to challenges in data accessibility and usability. A systematic review of 60 peer-reviewed articles on marine metabarcoding studies revealed barriers such as the absence of common context and vocabulary, missing metadata, limitations in supplementary information, and a concentration of research in the United States. Small choices made by authors and journals can greatly impact the discoverability and reusability of data. Despite these challenges, there is consistency and creativity in data storage choices, as well as a trend towards open access publishing.
Article
Multidisciplinary Sciences
Alexandria B. Boehm, Marlene K. Wolfe, Krista R. Wigginton, Amanda Bidwell, Bradley J. White, Bridgette Hughes, Dorothea Duong, Vikram Chan-Herur, Heather N. Bischel, Colleen C. Naughton
Summary: We measured the concentrations of various viruses in wastewater solids at twelve wastewater treatment plants in Central California, USA, including SARS-CoV-2, influenza A and B viruses, respiratory syncytial virus (RSV), mpox virus, human metapneumovirus, norovirus GII, and pepper mild mottle virus nucleic acids. Measurements were conducted daily for up to two years, following best practices for making environmental molecular biology measurements using digital droplet (reverse-transcription-) polymerase chain reaction (RT-PCR). These data can provide insights into disease occurrence in communities contributing to the wastewater.
Article
Engineering, Environmental
Laura Roldan-Hernandez, Alexandria B. Boehm
Summary: Despite the widespread adoption of wastewater surveillance, more research is needed to understand the fate and transport of viral genetic markers in wastewater. This study examined the solid-liquid partitioning behavior of four viruses in wastewater and found that RNA concentrations were higher in the solid fraction than the liquid fraction by approximately 3-4 orders of magnitude.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Environmental Sciences
Alexandria B. Boehm, Marlene K. Wolfe, Bradley J. White, Bridgette Hughes, Dorothea Duong, Niaz Banaei, Amanda Bidwell
Summary: This study aims to investigate whether HuNoV GII preferentially adsorbs to wastewater solids and to explore the concentrations of HuNoV GII in wastewater solids in wastewater treatment plants across the United States. The study also aims to develop and apply a mass-balance model that predicts the fraction of individuals shedding HuNoV based on measured concentrations in wastewater solids.
JOURNAL OF EXPOSURE SCIENCE AND ENVIRONMENTAL EPIDEMIOLOGY
(2023)
Article
Engineering, Environmental
Lena Mutzner, Kefeng Zhang, Richard G. Luthy, Hans Peter H. Arp, Stephanie Spahr
Summary: Urban stormwater, once managed primarily for flood control and property protection, is now increasingly recognized as an underutilized water resource. However, conventional stormwater treatment systems are inefficient at removing persistent and toxic organic substances, which may pose risks when urban runoff is used for drinking water sources or discharged into surface waters. By reviewing numerous stormwater monitoring studies, we identified 49 persistent, mobile and toxic (PMT) and very persistent, very mobile (vPvM) substances in urban stormwater. The lack of surveillance data on these substances and their fate in stormwater control measures hinders reliable risk assessments of stormwater capture. Unified guidelines are needed to monitor and assess the risks of PMT/vPvM substances in urban stormwater runoff and establish improved stormwater management criteria for safe water supply.
ENVIRONMENTAL SCIENCE-WATER RESEARCH & TECHNOLOGY
(2023)
Review
Environmental Sciences
Andrew Mertens, Benjamin F. Arnold, Jade Benjamin-Chung, Alexandria B. Boehm, Joe Brown, Drew Capone, Thomas Clasen, Erica Fuhrmeister, Jessica A. Grembi, David Holcomb, Jackie Knee, Laura H. Kwong, Audrie Lin, Stephen P. Luby, Rassul Nala, Kara Nelson, Sammy M. Njenga, Clair Null, Amy J. Pickering, Mahbubur Rahman, Heather E. Reese, Lauren Steinbaum, Jill Stewart, Ruwan Thilakaratne, Oliver Cumming, John M. Colford, Ayse Ercumen
Summary: This article conducts a systematic review and analysis to examine the effects of water, sanitation, and hygiene interventions on the presence of pathogens and faecal contamination in the environment. The findings show that while these interventions can reduce pathogen detection, they have limited impact on human and animal faecal markers. It suggests that the implemented sanitation measures may not adequately address human waste and reduce exposure to enteropathogens in the environment.
LANCET PLANETARY HEALTH
(2023)
Article
Engineering, Environmental
Mary E. Schoen, Amanda L. Bidwell, Marlene K. Wolfe, Alexandria B. Boehm
Summary: IAV RNA in wastewater settled solids can serve as an indicator for tracking the onset and peak of influenza season, providing early warning and helping inform vaccination and intervention strategies.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Engineering, Environmental
Laura Roldan-Hernandez, Alexandria B. Boehm
Summary: Despite the widespread adoption of wastewater surveillance, more research is needed to understand the fate and transport of viral genetic markers in wastewater. In this study, the solid-liquid partitioning behavior of four viruses in wastewater was examined using laboratory experiments and endogenous viruses in raw wastewater. The results showed that the RNA concentrations of the viruses were higher in the solid fraction than the liquid fraction by approximately 3-4 orders of magnitude. Further research is needed to understand how virus and wastewater characteristics might influence the partitioning of viral genetic markers in wastewater.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)