期刊
ENVIRONMENTAL SCIENCE & TECHNOLOGY
卷 49, 期 24, 页码 14605-14613出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.est.5b03988
关键词
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资金
- Department of Forestry and Natural Resources, Purdue University, IN
- Indiana Genomic Initiative at Indiana University (INGEN)
- Lilly Endowment, Inc.
Daphnia pulex is a widely used toxicological model and is known for its sensitivity to cadmium (Cd). Recent research suggests that microRNAs (miRNAs) play a critical role in animal responses to heavy metals. To investigate the functions of D. pulex miRNAs under Cd exposure, we analyzed the miRNA profiles of D. pulex after 48 h using miRNA microarrays and validated our findings by q-PCR. miRNA dpulet-7 was identified as a stably expressed gene and used as a reference. We identified 22 and 21 differentially expressed miRNAs under low (20 mu g/L CdCl2) and high-exposure (40 mu g/L CdCl2) concentrations compared to controls, respectively. Cellular functions of predicted miRNA target Cd-responsive genes included oxidative stress, ion transport, mitochondrial damage, and DNA repair. An insulin-related network was also identified in relation to several Cd-responsive miRNAs. The expression of three predicted target genes for miR-71 and miR-210 were evaluated, and expression of two of them (SCN2A and SLC31A1) was negatively correlated with the expression of their regulator miRNAs. We show miR-210 is hypoxia-responsive in D. pulex and propose Cd and hypoxia induce miR-210 via a same HIF1 alpha modulated pathway. Collectively, this research advances our understanding on the role of miRNAs in response to heavy-metal exposure.
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