期刊
ENVIRONMENTAL SCIENCE & TECHNOLOGY
卷 49, 期 24, 页码 14570-14578出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.est.5b03838
关键词
-
资金
- Australian Research Council [DE130101046]
- RMIT
- French Ministry of Economy, Industry and Digital
- Australian Research Council [DE130101046] Funding Source: Australian Research Council
The fish embryo toxicity (PET) biotest has gained popularity as one of the alternative approaches to acute fish toxicity tests in chemical hazard and risk assessment. Despite the importance and common acceptance of FET, it is still performed in multiwell plates and requires laborious and time-consuming manual manipulation of specimens and solutions. This work describes the design and validation of a microfluidic Lab-on-a-Chip technology for automation of the zebrafish embryo toxicity test common in aquatic ecotoxicology. The innovative device supports rapid loading and immobilization of large numbers of zebrafish embryos suspended in a continuous microfluidic perfusion as a means of toxicant delivery. Furthermore, we also present development of a customized mechatronic automation interface that includes a high-resolution USB microscope, LED cold light illumination, and miniaturized 3D printed pumping manifolds that were integrated to enable time-resolved in situ analysis of developing fish embryos. To investigate the applicability of the microfluidic PET (mu FET) in toxicity testing, copper sulfate, phenol, ethanol, caffeine, nicotine, and dimethyl sulfoxide were tested as model chemical stressors. Results obtained on a chip-based system were compared with static protocols performed in microtiter plates. This work provides evidence that PET analysis performed under microperfusion opens a brand new alternative for inexpensive automation in aquatic ecotoxicology.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据