Review
Chemistry, Analytical
Jincymol Kappen, Malgorzata Skorupa, Katarzyna Krukiewicz
Summary: The detection of cancer biomarkers, especially using electrochemical biosensors based on conducting polymers (CPs) and their composites, has shown great promise in early diagnosis, treatment response determination, and disease progression monitoring. These CP-based biosensors possess high sensitivity and specificity due to the large surface area, porosity, and functional groups of CPs. They allow for the capture and detection of cancer biomarkers, such as DNAs, miRNAs, proteins, enzymes, neurotransmitters, and whole cancer cells, with significantly enhanced sensitivity and a limit of detection as low as 0.5 fM for miRNA and 10 cells for cancer cell detection. Multiplex biomarker detection and cell capture methods are considered the most promising category, offering more accurate and reliable results. This review provides a comprehensive summary of CP-based electrochemical biosensors and their potential applications in cancer diagnosis and treatment.
Article
Engineering, Electrical & Electronic
Marcin Kowalski, Mateusz Brodowski, Karolina Dziabowska, Marta Skwarecka, Mateusz Ficek, Dawid Nidzworski, Robert Bogdanowicz
Summary: This study reports the development of an electrochemical immunosensor for the detection of bacteria from the Pseudomonas genus. The sensor demonstrated high sensitivity and specificity, and utilized boron-doped carbon nanowalls as the sensing material. The study highlighted the importance of material choice and modification procedure in determining sensor performance.
IEEE SENSORS JOURNAL
(2022)
Article
Polymer Science
Adel Yavarinasab, Mostafa Abedini, Hamed Tahmooressi, Sajjad Janfaza, Nishat Tasnim, Mina Hoorfar
Summary: This study analyzed the application of potentiodynamic electrochemical impedance spectroscopy (PDEIS) for the selective in situ recognition of biological trace elements. An electrochemical sensor was developed using electropolymerization of aniline (Ani) on the surface of homemade pencil graphite electrodes (PGE). The results showed the potential of PDEIS as a complementary tool for metallic ion sensing.
Article
Polymer Science
Alexey I. I. Volkov, Rostislav V. V. Apraksin
Summary: In this study, the effects of different salt additives on the electrochemical properties, morphology, and structure of PEDOT:PSS films were systematically investigated. The results showed a close relationship between the electrochemical properties of the films and the nature of the additives, which could be correlated with the Hofmeister series. This research provides insights into the modification processes within PEDOT:PSS films and the potential for fine-tuning their properties by selecting appropriate salt additives.
Article
Engineering, Electrical & Electronic
Stefano Bonaldo, Erica Cretaio, Elisabetta Pasqualotto, Matteo Scaramuzza, Lara Franchin, Sara Poggi, Alessandro Paccagnella
Summary: This study presents a cheap biosensing method for rapid detection of Lactococcus lactis phages. The detection is based on live bacteria covering the sensor electrodes, and the electrical response is measured by electrochemical impedance spectroscopy. Experimental results show that the proposed method outperforms other methods in terms of detection capability and time.
IEEE SENSORS JOURNAL
(2023)
Review
Chemistry, Multidisciplinary
Alvaro Teran-Alcocer, Francisco Bravo-Plascencia, Carlos Cevallos-Morillo, Alex Palma-Cando
Summary: Electrochemical sensors based on conducting polymers are low-cost, rapid, and versatile for detecting analytes in liquid solutions. The focus is on the synthetic processes, morphological characterization, and nanostructure formation to enhance sensitivity for detecting molecules in the human body and water pollutants. Polypyrroles, polyanilines, and polythiophenes are commonly used conducting polymers for building electrochemical sensors.
Article
Engineering, Electrical & Electronic
Juntao Chen, Ning Lu, Xu Wang, Yangzi Chen, Miao Guo, Ying Xu
Summary: Antibiotic detection can be done through direct detection or indirect detection. In this study, a novel electrode modification method using electrochemical impedance measurement was introduced for accurate quantification of antibiotic concentration based on bacterial proliferation. The experimental results showed high accuracy in antibiotic prediction, providing a better method for accurate antibiotic evaluation.
IEEE SENSORS JOURNAL
(2022)
Article
Electrochemistry
Serge Kernbach
Summary: The para- and ortho-isomers of water and dissolved molecular oxygen have different spin configurations and can be excited to change macroscopically measurable properties of aqueous solutions. This study characterizes the ionic dynamics and surface tension effects of dissolving carbon dioxide and hydrogen peroxide in pure water using electrochemical impedance spectroscopy. The results show distinguishable reaction pathways and anomalous quasi-periodical fluctuations, indicating potential applications in affordable electrochemical sensors and quantum biology.
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
(2022)
Article
Chemistry, Multidisciplinary
Tina Skorjanc, Andraz Mavric, Mads Nybo Sorensen, Gregor Mali, Changzhu Wu, Matjaz Valant
Summary: This study synthesized a novel cationic covalent organic polymer (COP) named CATN, which showed potential for interacting with negatively charged bacterial cell surfaces through electrostatic interactions. The CATN-coated interdigitated electrode arrays were used in two-electrode electrochemical impedance spectroscopy (EIS), and the system showed sensitivity to bacterial concentrations as low as 2 CFU mL(-1).
Article
Chemistry, Analytical
Wouter Stilman, Derick Yongabi, Soroush Bakhshi Sichani, Florian Thesseling, Olivier Deschaume, Tristan Putzeys, Tiago C. Pinto, Kevin Verstrepen, Carmen Bartic, Michael Wubbenhorst, Marc Heyndrickx, Patrick Wagner
Summary: A new technique combining ultrathin surface-imprinted polymer layers as receptors with impedance spectroscopy has been developed for the sensitive and rapid detection of Saccharomyces strains in complex food matrices. The study demonstrated strong selectivity, and the results for yeast were found to be transferable to other microorganisms.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Biochemical Research Methods
Pablo C. Serrano, Gisele E. Nunes, Lindiomar B. Avila Jr, Carleane P. S. Reis, Aldo M. C. Gomes, Francoise T. Reis, Maria L. Sartorelli, Silvia Pedroso Melegari, William G. Matias, Ivan H. Bechtold
Summary: Saxitoxin, a harmful cyanotoxin, requires monitoring in water bodies. An electrochemical impedimetric biosensor using a specific aptamer was developed for its detection, showing high sensitivity and selectivity.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Engineering, Chemical
Marielle DuToit, Edgard Ngaboyamahina, Mark Wiesner
Summary: In this study, protein fouling within a polymeric microfiltration membrane was characterized in real time using nonFaradaic electrochemical impedance spectroscopy. By modeling the electrochemical system with equivalent circuit components, both qualitative and quantitative monitoring of protein fouling was achieved, marking the first attempt to deploy a conductive polymeric membrane as an active electrode in an EIS setup.
JOURNAL OF MEMBRANE SCIENCE
(2021)
Article
Chemistry, Multidisciplinary
Gideon Wackers, Peter Cornelis, Tristan Putzeys, Marloes Peeters, Jan Tack, Freddy Troost, Theodor Doll, Nicolas Verhaert, Patrick Wagner
Summary: The article discusses the development of a catheter-based, biomimetic sensor for quantifying histamine levels in human bowel fluid, aiming to become a minimally invasive diagnostic tool for functional bowel disorders. The sensor utilizes molecularly imprinted polypyrrole coatings for impedimetric sensing electrodes, showing negligible cross-sensitivity to competitor molecules and primarily affecting the resistive component of the electrode-liquid interface. This sensor integrated into a catheter can measure histamine concentrations in real-time, providing potential benefits for diagnostic tests.
Article
Chemistry, Analytical
Pia Binder, Franziska V. Oberhaus
Summary: Self-assembled monolayers (SAMs) are widely used for various applications, but SAMs that can effectively inhibit nonspecific binding for electrochemical sensors have not been developed. In this study, diglycolamine was introduced for modifying electrodes with outstanding antifouling performance. The sensor design, consisting of diglycolamine and an aptamer, is easy to prepare, low-cost, and highly specific. The method can be applied to various detection techniques and has the potential to change the way we analyze complex biological samples.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Abd-Elgawad Radi, Reham Oreba, Reda Elshafey
Summary: A reusable molecularly imprinted polypyrrole (MIP(O-PPy)) electrode was developed to detect profenofos in food, with optimized experimental parameters and excellent recognition performance.
Article
Engineering, Electrical & Electronic
V. Yu Nesterov, O. Sokolovskaya, L. A. Golovan, D. Shuleiko, A. Kolchin, D. E. Presnov, P. K. Kashkarov, A. Khilov, D. A. Kurakina, M. Yu Kirillin, E. A. Sergeeva, S. Zabotnov
Summary: The possibility of manufacturing silicon nanoparticles by picosecond laser fragmentation of silicon microparticles in water is analyzed. The average particle sizes are found to have a nonmonotonic dependence on the initial mass concentration of the micropowder. Simulations show that fragmentation occurs in different regions depending on the concentration, with deeper propagation resulting in fragmentation in the paraxial region while higher concentrations lead to surface fragmentation due to strong extinction. Spectrophotometry measurements indicate the potential use of these fragmented nanoparticles in optical imaging of biological objects.
QUANTUM ELECTRONICS
(2022)
Article
Physics, Applied
Y. A. Ovchenkov, D. A. Chareev, E. S. Kozlyakova, E. E. Levin, M. G. Mikheev, D. E. Presnov, A. S. Trifonov, O. S. Volkova, A. N. Vasiliev
Summary: Our study on FeSe1-xTex crystals with x < 0.15 reveals phase separation into phases with different stoichiometry of iron. This phase separation suggests structural instability of the iron plane within the studied compositions. We tentatively propose an explanation for this structural instability based on possible changes in bond polarity and the unique d-d exchange in the iron plane.
SUPERCONDUCTOR SCIENCE & TECHNOLOGY
(2022)
Review
Chemistry, Analytical
Maria A. Komkova, Arkady A. Karyakin
Summary: This review discusses the pathway from advanced electrocatalysts to nanozymes that can outperform natural enzymes. Catalytically synthesized Prussian blue nanoparticles, which have high catalytic rate constants, are the best candidates for mimicking peroxidase activity. These nanoparticles, referred to as nanozymes, exhibit enzymatic specificity and can be used to modify non-traditional materials. By covering the Prussian blue core with a nickel hexacyanoferrate shell, the resulting core-shell nanozymes surpass natural enzymes in terms of activity. The study also found that nanozymes have a dramatically improved bimolecular rate constants compared to biological catalysts.
Article
Biochemistry & Molecular Biology
Ilya Lyagin, Nikolay Stepanov, Denis Presnov, Artem Trifonov, Elena Efremenko
Summary: The self-assembling of nanosized materials is a promising field for research and development, and various approaches are used to obtain inorganic, organic, and composite nanomaterials with different functionality. In this study, self-assembling nanocomplexes were prepared using enzymes and polypeptides, and their properties were investigated using low-molecular weight biologically active compounds. Molecular modeling was used to screen the possible formation of catalytically active self-assembling nanocomplexes, and two enzymes and two compounds were selected for further characterization. These nanocomplexes showed significant improvement in the antibacterial activity of colistin and polymyxin B against both Gram-positive and Gram-negative bacteria.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Physics, Applied
Y. A. Ovchenkov, D. A. Chareev, D. E. Presnov, O. S. Volkova, A. N. Vasiliev
Summary: In this study, the transport properties of FeSe1-xTex and pure FeSe crystals were compared. The results showed significant differences in structural transitions and temperature dependence between the two, suggesting a change in the ground state and the presence of quantum criticality. Furthermore, the phase diagram of FeSe1-xTex at low x resembled that of FeSe under pressure.
JOURNAL OF SUPERCONDUCTIVITY AND NOVEL MAGNETISM
(2023)
Article
Chemistry, Analytical
Galina V. V. Presnova, Gleb A. A. Zhdanov, Luibov Yu. Filatova, Mariya M. M. Ulyashova, Denis E. E. Presnov, Maya Yu. Rubtsova
Summary: Gold nanoparticles (AuNPs) are used to enhance color intensity for DNA membrane microarrays through seed-mediated growth. The method involves hybridization of biotin-labeled DNA with capture oligonucleotide probes, followed by detection of AuNPs through a streptavidin-AuNP conjugate. The optimized seed-mediated enlargement of AuNPs resulted in larger particles and improved signal/background ratio.
Article
Chemistry, Multidisciplinary
M. A. Komkova, R. G. Valeev, Y. G. Kolyagin, E. A. Andreev, A. N. Beltukov, V. N. Nikitina, A. K. Yatsimirsky, A. A. Karyakin, A. A. Eliseev
Summary: This study provides a comprehensive investigation of chemical states in boronate-substituted polyaniline (PANI) under different pH and potential conditions. The results show that the boronate substituent allows for a high proportion of protonated amino groups in neutral and basic media, leading to increased electroactivity and conductivity of the polymer. These findings open up new possibilities for sensing and electrocatalysis research.
MATERIALS TODAY CHEMISTRY
(2022)
Article
Chemistry, Analytical
Maria A. Komkova, Andrei A. Eliseev, Artem A. Eliseev, Valeria S. Kasimovskaya, Andrei A. Poyarkov, Arkady A. Karyakin
Summary: We propose pulse power generation (PPG) amperometry as an advanced readout for Prussian blue (PB)-based (bio)sensors. The suggested pulse regime, which involves periodic opening and shorting of the circuit, provides a 15-fold increased signal-to-background ratio compared to the conventional continuous short-circuiting mode. The improved sensitivity and stability of the pulse readout allow reliable monitoring of blood glucose variations through sweat analysis.
ANALYTICAL CHEMISTRY
(2023)
Article
Agricultural Engineering
Svetlana Shekhurdina, Elena Zhuravleva, Andrey Kovalev, Egor Andreev, Emil Kryukov, Natalia Loiko, Alexandra Laikova, Nadezhda Popova, Dmitriy Kovalev, Vivekanand Vivekanand, Yuriy Litti
Summary: Conductive materials and dielectric materials were investigated to study the impact of direct interspecies electron transfer (DIET) on methane production from highly concentrated volatile fatty acids. Stainless-steel mesh and carbon felt significantly improved methane yield and production rate compared to control and dielectric materials. The promotion of DIET by conductive materials is influenced by various factors, including the specificity of electrogenic groups to the material surface.
BIORESOURCE TECHNOLOGY
(2023)
Article
Chemistry, Analytical
Vita N. Nikitina, Elena V. Daboss, Darya V. Vokhmyanina, Ivan D. Solovyev, Egor A. Andreev, Maria A. Komkova, Arkady A. Karyakin
Summary: We propose a novel approach to increase the upper limit of linearity in glucose test strips by using membrane-forming mixtures for co-immobilization of enzymes and mediators in polymer matrices, which results in a calibration range of up to 50 mM.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Maria A. Komkova, Vera A. Shavokshina, Alexander A. Zarochintsev, Denis M. Melnik, Ilya O. Aparin, Timofei S. Zatsepin, Arkady A. Karyakin
Summary: We propose catalytically synthesized nanozymes based on Prussian Blue (PB) and azidomethyl-substituted poly (3,4-ethylenedioxythiophene) (azidomethyl-PEDOT) as novel electrocatalytic labels for DNA/RNA sensors. The electrocatalytic labels enable direct electrocatalysis of H2O2 reduction, allowing robust detection of target sequences with concentrations below 0.2 nM in blood serum within an hour. We believe that this technology sets new avenues for point-of-care DNA/RNA sensing.
Article
Chemistry, Multidisciplinary
Andrei A. Eliseev, Konstantin E. Gurianov, Andrei. A. Poyarkov, Maria A. Komkova, Ilia S. Sadilov, Andrei P. Chumakov, Dmitrii I. Petukhov
Summary: This paper presents a comparative study on the swelling processes of free-standing graphene oxide membranes and GO laminates encapsulated with epoxy glue. The research reveals that the d-spacing of free-standing graphene oxide membranes can vary greatly depending on the ambient humidity and electrolyte type and concentration. On the other hand, the swelling of epoxy-encapsulated GO is limited. The physical constriction and intercalation of amines into GO laminates are believed to be the causes of this swelling suppression.
Review
Chemistry, Multidisciplinary
Arkady A. Karyakin
Summary: This paper critically reviews the catalytic properties of peroxidase mimicking nanozymes. The main factors affecting nanoparticles activity are discussed and the catalytic properties are normalized for comparison. Nitrogen-coordinated iron atoms have the highest catalytic activities in hydrogen peroxide reduction. However, metal/metal oxide and 'single atom' nanozymes have additional activities that impair their application abilities. Nanoparticles synthesized from Prussian Blue display enzymatic selectivity in addition to high catalytic activity.
RUSSIAN CHEMICAL REVIEWS
(2023)
Article
Chemistry, Analytical
Maria A. Komkova, Anna S. Alexandrovich, Arkady A. Karyakin
Summary: This study reports the drop-cast production of glucose biosensors using pyrroloquinoline quinone dependent glucose dehydrogenase (PQQ GDH) for efficient bioelectrocatalysis. Poly(Methylene Green) (p(MG)) nanoparticles were used as anchors for enzyme immobilization. The resulting biosensors showed advantageous characteristics and high sensitivity, making them suitable for non-invasive diabetes monitoring.
Article
Materials Science, Multidisciplinary
Ilya Lyagin, Olga Maslova, Nikolay Stepanov, Denis Presnov, Elena Efremenko
Summary: Composite fiber materials with antibacterial properties were obtained by modifying and functionalizing bacterial cellulose, polylactide, and usual fibers. The addition of natural polymers and nanoparticles significantly improved the antibacterial activity.
