期刊
REVISTA DA SOCIEDADE BRASILEIRA DE MEDICINA TROPICAL
卷 50, 期 1, 页码 92-98出版社
SOC BRASILEIRA MEDICINA TROPICAL
DOI: 10.1590/0037-8682-0316-2016
关键词
Rat lungworm; Giant African snail; Eosinophilic meningitis; Nematode; Emerging parasitosis
资金
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2011/05893-8]
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [11/05893-8] Funding Source: FAPESP
Introduction: Angiostrongylus cantonensis causes eosinophilic meningoencephalitis in humans. Worldwide expansion of this nematode is linked to the dispersion of their hosts. This study aimed to determine the prevalence of A. cantonensis infection in Achatina fulica in the nine municipalities that make up Baixada Santista, Sao Paulo, Brazil. Methods: Angiostrongylus cantonensis larvae were analyzed using optical microscopy. We performed polymerase chain reaction and restriction fragment length polymorphism using restriction endonuclease ClaI, directed to the internal transcribed spacer region 2 of A. cantonensis larval DNA. Results: Of the 540 snails analyzed, 117 (21.7%) were infected by A. cantonensis. For morphological and morphometric analyses, 60 larvae were used. Second-stage larvae were, on average, 358.2 mu m long and 26.4 mu m wide, while third-stage larvae were, on average, 450 mu m long and 21.12 mu m wide. The tails of the larvae ended in a fine tip. Conclusions: All municipalities comprising Baixada Santista had A. fulica that were naturally infected with A. cantonensis. All of the observed characteristics were typical of the species.
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