Article
Microbiology
Yimei Cao, Kun Li, Xiangchuan Xing, Guoqiang Zhu, Yuanfang Fu, Huifang Bao, Xingwen Bai, Pu Sun, Pinghua Li, Jing Zhang, Xueqing Ma, Jian Wang, Zhixun Zhao, Dong Li, Zaixin Liu, Zengjun Lu
Summary: In this study, a bovine broadly neutralizing monoclonal antibody (W145) against FMDV serotype A was successfully produced using fluorescence-based single-B-cell antibody technology. A competitive enzyme-linked immunosorbent assay (NAC-ELISA) was developed for the detection of neutralizing antibodies against FMDV serotype A. The NAC-ELISA showed high specificity and sensitivity and could be used to evaluate protective immunity.
JOURNAL OF CLINICAL MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Sukyo Jeong, Hyun Joo Ahn, Kyung Jin Min, Jae Won Byun, Hyun Mi Pyo, Mi Young Park, Bok Kyung Ku, Jinju Nah, Soyoon Ryoo, Sung Hwan Wee, Sang Jick Kim
Summary: A phage-displayed scFv library was constructed from FMDV-immune cattle, resulting in specific and high-affinity scFv antibodies for FMDV detection. Using natural host-derived antibodies such as bovine scFv in cELISA showed feasibility and effectiveness in serological detection of antibody response to FMDV in susceptible animals.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Virology
Liting Yan, Lijun Sun, Chunyan Guo, Lanlan Li, Jingying Sun, Xiaoyan Huang, Penghua Zhao, Xin Xie, Jun Hu
Summary: This study evaluated the neutralizing and hemagglutination inhibition activity of a murine anti-H1N1 monoclonal antibody, and identified its epitope peptide through phage display technique. The identified epitope contains key amino acids for sialic acid receptor binding in HA, contributing to the production of neutralizing antibodies.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Article
Biochemistry & Molecular Biology
Rui Chen, Yimin Wen, Enbo Yu, Junpeng Yang, Yixiao Liang, Daili Song, Yiping Wen, Rui Wu, Qin Zhao, Senyan Du, Qigui Yan, Xinfeng Han, Sanjie Cao, Xiaobo Huang
Summary: We identified two monoclonal antibodies that effectively neutralized PDCoV, with one targeting the S280-288 epitope and the other targeting the S506-513 epitope. The S280-288 epitope induced specific neutralizing antibodies and provided partial protection against disease in piglets. This study provides valuable insights for understanding the pathogenic mechanism of PDCoV, vaccine design, and antiviral drug development.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biotechnology & Applied Microbiology
Kun Li, Guoqiang Zhu, Shasha Zhou, Pu Sun, Hengmei Wang, Huifang Bao, Yuanfang Fu, Pinghua Li, Xingwen Bai, Xueqing Ma, Jing Zhang, Dong Li, Yingli Chen, Zaixin Liu, Yimei Cao, Zengjun Lu
Summary: Pigs are susceptible to foot-and-mouth disease virus (FMDV), and the humoral immune response plays a crucial role in protection. Antibodies from pigs recognize conserved antigen epitopes on capsid protein VP2 and exhibit broad reactivity against both FMDV serotypes A and O. The study reveals a novel method for analyzing the antibody response against FMDV in pigs at the clonal level.
JOURNAL OF GENERAL VIROLOGY
(2021)
Article
Virology
Yong He, Kun Li, Li Wang, Zixian Sun, Yimei Cao, Pinghua Li, Pu Sun, Huifang Bao, Shasha Zhou, Sheng Wang, Xingwen Bai, Xuerong Liu, Lixia Zhao, Xiuli Fan, Zaixin Liu, Zengjun Lu, Cheng Yang, Zhiyong Lou
Summary: This study provides important insights into the antigenic diversity and neutralizing antibodies of foot-and-mouth disease virus, demonstrating the cross-reactions of the R55 antibody with FMDV-AWH and FMDV-AAF, as well as its neutralizing effect on FMDV-AWH.
JOURNAL OF VIROLOGY
(2021)
Article
Virology
Kun Li, Yong He, Li Wang, Pinghua Li, Sheng Wang, Pu Sun, Huifang Bao, Yimei Cao, Xuerong Liu, Guoqiang Zhu, Yali Song, Xingwen Bai, Xueqing Ma, Yuanfang Fu, Hong Yuan, Jing Zhang, Jian Wang, Yingli Chen, Dong Li, Zhiyong Lou, Zaixin Liu, Zengjun Lu
Summary: Foot-and-mouth disease virus (FMDV) is a highly contagious virus that infects cloven-hoofed animals. In this study, researchers identified two cross-protective antigen sites on VP2 and VP3 using bovine-derived broadly neutralizing monoclonal antibodies. One site on VP3 was recognized by bnAb C4, while the other site on VP2 was recognized by 9 different bnAbs.
JOURNAL OF VIROLOGY
(2021)
Article
Microbiology
Sun Young Park, Jong Sook Jin, Dohyun Kim, Jae Young Kim, Sang Hyun Park, Jong-Hyeon Park, Choi-Kyu Park, Young-Joon Ko
Summary: Foot-and-mouth disease (FMD) is a highly contagious vesicular disease that affects cloven-hoofed animals and often causes enormous economic loss in the livestock industry. In this study, researchers generated VP0-specific monoclonal antibodies (mAbs) and confirmed their potential role in FMDV replication and the mechanism underlying VP0 maturation.
Article
Immunology
Lei Chen, Jiansheng Lu, Junjie Yue, Rong Wang, Peng Du, Yunzhou Yu, Jiazheng Guo, Xi Wang, Yujia Jiang, Kexuan Cheng, Zhixin Yang, Tao Zheng
Summary: A humanized monoclonal antibody 9-8-h2, derived from mice immunization, showed potent neutralization activity against HAdV55. It recognized a conformational neutralization epitope and had favorable physicochemical properties. It could be a promising molecule for the prevention and treatment of HAdV55.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Mohammad Ridhuan Mohd Ali, Jia Siang Sum, Nurul Najian Aminuddin Baki, Yee Siew Choong, Nur Asyura Nor Amdan, Fairuz Amran, Theam Soon Lim
Summary: This study aimed to develop specific monoclonal antibodies against LipL21 antigen for future use in rapid and accurate immunoassay for leptospirosis. The generated monoclonal antibody against rLipL21 will be evaluated as a detection reagent for the diagnosis of human leptospirosis in a future study.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Review
Microbiology
Fenghao Peng, Naijing Hu, Yingjun Liu, Cong Xing, Longlong Luo, Xinying Li, Jing Wang, Guojiang Chen, He Xiao, Chenghua Liu, Beifen Shen, Jiannan Feng, Chunxia Qiao
Summary: Smallpox is an infectious disease caused by the variola virus, with a high mortality rate. Although smallpox was declared eradicated in 1980, the potential threat of bioterrorism has led to resumed research on the treatment and prevention of smallpox.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Zhengwu Chang, Xiaoyu Dong, Zhao Guan, Kejia Lu, Xi Chen, Xi Wei, Hanwei Guo, Ruyi Dang, Juan Wang, Xinglong Wang, Sa Xiao, Zengqi Yang, Haijin Liu
Summary: Vaccines are widely used to prevent Newcastle disease virus. A study found that epitope mutations in the virus may decrease the effectiveness of the vaccine, but their contribution to antigenic mutation and immune escape is still controversial. The study also showed that a matched vaccine, generated through modification of the virus protein, was more effective in inhibiting viral shedding and tissue damage compared to an unmatched vaccine. However, both vaccines induced similar levels of neutralizing antibodies in vaccinated chickens.
VETERINARY MICROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Jingru Liang, Hang Dong, Fei Xu, Baowei Li, Haimei Li, Limei Chen, Mei Li, Yingchu Liu, Guosheng Jiang, Jinhua Dong
Summary: In this study, a monoclonal antibody named ESC9 was developed, and an immunosensor called ESC9 Quenchbody (Q-body) was generated for rapid and sensitive detection of estradiol. The ESC9 Q-body showed potential for clinical applications due to its excellent detection speed and sensitivity.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Immunology
Vincent Dussupt, Kayvon Modjarrad, Shelly J. Krebs
Summary: The unprecedented Zika outbreak in the Americas from 2015-2016 led to global concern and accelerated the deployment of vaccine and therapeutic countermeasures. Several potent neutralizing antibodies against Zika and related flaviviruses have been discovered in recent years. Structure-based approaches have helped identify species-specific and cross-protective epitopes with therapeutic value.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Virology
Wei Wen, Xinghua Chen, Qiang Lv, Huanchun Chen, Ping Qian, Xiangmin Li
Summary: Researchers have produced five monoclonal antibodies that can detect SVV infection, and these antibodies exhibit high neutralizing titers to SVV. The smallest linear neutralizing epitope (153)QELNEE(158) has been identified. These monoclonal antibodies and identified epitopes are important for further research on VP2 structure and function, as well as the development of diagnostic methods for different SVV strains. Moreover, the epitope recognized by these monoclonal antibodies against VP2 protein may provide insights for the development of novel SVV vaccines and oncolytic viruses.
Article
Virology
Chao Sun, Mengmeng Liu, Jitao Chang, Decheng Yang, Bo Zhao, Haiwei Wang, Guohui Zhou, Changjiang Weng, Li Yu
JOURNAL OF VIROLOGY
(2020)
Article
Virology
Decheng Yang, Chao Sun, Rongyuan Gao, Haiwei Wang, Wenming Liu, Kewei Yu, Guohui Zhou, Bo Zhao, Li Yua
JOURNAL OF VIROLOGY
(2020)
Article
Virology
Wenming Liu, Decheng Yang, Chao Sun, Haiwei Wang, Bo Zhao, Guohui Zhou, Li Yu
JOURNAL OF VIROLOGY
(2020)
Article
Virology
Chen Li, Jiabao Shi, Haiwei Wang, Efrain E. Rivera-Serrano, Decheng Yang, Guohui Zhou, Chao Sun, Craig E. Cameron, Li Yu
Summary: This study demonstrates the importance of FMDV genetic diversity in viral virulence and transmissibility in susceptible animals. The generation of a high-fidelity FMDV variant with a single amino acid substitution in polymerase highlights the impact of viral population diversity on interhost viral spread. Furthermore, the high genetic diversity of type O FMDV may contribute to its dominance in the global epidemic by increasing both virulence and transmissibility.
JOURNAL OF VIROLOGY
(2021)
Article
Virology
Nana Wang, Haiwei Wang, Jiabao Shi, Chen Li, Xinran Liu, Junhao Fan, Chao Sun, Craig E. Cameron, Hong Qi, Li Yu
Summary: Senecavirus A (SVA) is a picornavirus that causes vesicular disease in swine and is the only member of its genus. The IRES in SVA plays a crucial role in cap-independent translation. Studies have shown that the stem-loop I element in SVA's IRES is essential for IRES activity and viral replication.
Article
Immunology
Junhao Fan, Peiyu Xiao, Dongni Kong, Xinran Liu, Liang Meng, Tongqing An, Xuehui Cai, Haiwei Wang, Li Yu
Summary: The study developed a method using a recombinant virus with inserted 6xHis tag to purify and concentrate SVA vaccine antigens, and demonstrated the feasibility of this approach without compromising virus growth and antigenicity.
Article
Microbiology
Xing-Yang Cui, Da-Song Xia, Xin-Yi Huang, Xiao-Xiao Tian, Tao Wang, Yong-Bo Yang, Gang Wang, Hai-Wei Wang, Yue Sun, Yi-Hong Xiao, Zhi-Jun Tian, Xue-Hui Cai, Tong-Qing An
Summary: A novel recombinant PRRSV was isolated and found to cause mild clinical symptoms and severe tissue lesions, with a higher viral shedding. Genomic surveillance revealed an increasing number of recombinant PRRSV in Shandong province since 2015.
VETERINARY MICROBIOLOGY
(2022)
Review
Microbiology
Haiwei Wang, Xingyang Cui, Xuehui Cai, Tongqing An
Summary: RNA recombination plays a vital role in the evolution of RNA viruses, contributing to the emergence of new viral lineages, expansion in host tropism, adaptations to new environments, and virulence and pathogenesis. Recent progress in understanding the triggers and mechanisms behind recombination in positive-strand RNA viruses has helped predict the probability and outcome of viral recombination events, and design viruses with reduced recombination frequency for the development of live attenuated vaccines.
FRONTIERS IN MICROBIOLOGY
(2022)
Review
Oncology
Dankun Luo, Haiwei Wang, Qiang Wang, Wenping Liang, Bo Liu, Dongbo Xue, Yang Yang, Biao Ma
Summary: Oncolytic viruses have the potential to selectively kill tumor cells and can be genetically engineered for improved therapeutic efficacy, making them a promising immunotherapy strategy against cancer.
FRONTIERS IN ONCOLOGY
(2022)
Article
Veterinary Sciences
Meiyu Jia, Mingxia Sun, Yan-Dong Tang, Yu-Yuan Zhang, Haiwei Wang, Xuehui Cai, Fandan Meng
Summary: This study demonstrated that pig ANTXR1 is a crucial receptor mediating Senecavirus A (SVA) infection and investigated the pathways for SVA entry into pig cells, revealing the essential role of cholesterol in this process. These findings provide potential targets for the development of antiviral drugs against SVA infection in the pig population.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Virology
Yanfei Gao, Haiwei Wang, Shanghui Wang, Mingxia Sun, Zheng Fang, Xinran Liu, Xuehui Cai, Yabin Tu
Summary: Soluble VP2 protein of PPV was efficiently expressed and self-assembled into VLPs, which were purified to obtain high-purity PPV-VLPs. The developed VLP-based I-ELISA is a simple, cost-effective, and efficient method for the diagnosis of clinical pig serum and plasma samples.
Article
Microbiology
Xingyang Cui, Dasong Xia, Xinyi Huang, Yue Sun, Mang Shi, Jianqiang Zhang, Ganwu Li, Yongbo Yang, Haiwei Wang, Xuehui Cai, Tongqing An
Summary: In this study, the genomic sequences of PRRSV-2 from 1991 to 2021 were analyzed, revealing the temporal and geographical distribution of recombinant PRRSVs and the location of recombination hot spots. It was found that recombinant PRRSVs based on the L1 backbone had higher replication capacity. These findings provide new insights into PRRSV recombination and the mechanism behind it.
MICROBIOLOGY SPECTRUM
(2022)
Article
Immunology
Mingxia Sun, Shanghui Wang, Zheng Fang, Man Zhao, Yanfei Gao, Tongqing An, Yabin Tu, Haiwei Wang, Xuehui Cai
Summary: A Sandwich ELISA method based on a monoclonal antibody was developed for the quality control of PCV2 VLPs vaccines with high accuracy.
Article
Microbiology
Hai-Fan Zhao, Liang Meng, Zhi Geng, Zeng-Qiang Gao, Yu-Hui Dong, Hai-Wei Wang, Heng Zhang
Summary: This study reveals that phospholipids can bind to SVA 3C(pro) and regulate its protease activity, thereby affecting the replication and infection capacity of Seneca virus in host cells.
Review
Medicine, Research & Experimental
Haiwei Wang, Yuchuan Luo, Haisheng Wang, Feifei Li, Fanyuan Yu, Ling Ye
Summary: Osteoporosis is a significant public health problem characterized by bone loss and deterioration of bone tissue. Classic pharmacological options can improve bone mineral density and resist fractures, but may have side effects. Therefore, research on new pathogenesis and potential therapeutic targets is important for the development of effective treatments. This study provides a comprehensive review of the current advances in osteoporosis, presenting mechanistic insights and updated therapies.
Article
Veterinary Sciences
R. Mischke, K. Rumstedt, S. O. Hungerbuehler, K. Rohn, M. Schmicke
Summary: Contrast-enhanced ultrasound (CEUS) can provide an additional tool for the diagnosis of hypothyroidism in dogs and support its differentiation from non-thyroidal illness. Sedation has limited impact on CEUS results.
RESEARCH IN VETERINARY SCIENCE
(2024)
Review
Veterinary Sciences
Lucas Petitemberte de Souza, William Borges Domingues, Eduardo Bierhals Blodorn, Leandro da Silva Nunes, Hadassa Gabriela Ortiz, Eliza Rossi Komninou, Vinicius Farias Campos
Summary: This study performed a systematic review of the differential expression of sperm microRNAs in bulls related to fertility. A total of 182 unique differentially expressed miRNAs were identified, among which 49 miRNAs were found in common between at least two studies. The results suggest that miRNAs such as miR-10a, -10b, -103, -15b, -122, -125b, -126-5p, -151-5p, -193a-5p, -196a, -27a-5p, and -99b may serve as potential universal biomarkers for assessing the reproductive potential of males.
RESEARCH IN VETERINARY SCIENCE
(2024)
Article
Veterinary Sciences
Wonhee Cha, Katinca Fungbrant, Giulio Grandi, Ylva Persson
Summary: This study investigated the prevalence and development of hair loss in outdoor cattle in Sweden. It found a relatively high hair loss rate, with the occurrence of hair loss increasing over time during winters, suggesting a contagious etiology. The study also identified preventative delousing before November, keeping animals clean, and maintaining small group sizes as effective measures to reduce the risk of hair loss, while older age and access to bedding materials increased the risk.
RESEARCH IN VETERINARY SCIENCE
(2024)
Article
Veterinary Sciences
Sheeba Thomas, David Francis Lappin, David Bennett, Christopher Nile, Marcello Pasquale Riggio
Summary: This study found that Feline odontoclastic resorptive lesion (FORL) can be divided into two subgroups, with FORL-1 subgroup having a less diverse oral microbiome and higher levels of proinflammatory cytokines and chemokines, which may be associated with the severity and activity of the disease.
RESEARCH IN VETERINARY SCIENCE
(2024)
Article
Veterinary Sciences
S. L. Gayathri, M. Bhakat, T. K. Mohanty
Summary: This study used infrared thermography (IRT) to identify mastitis in Sahiwal cows. The results showed significant differences in the mean values of udder and teat surface temperature between healthy, subclinical mastitis (SCM), and clinical mastitis (CM) cases during different seasons. IRT is an efficient tool for the early identification of subclinical mastitis.
RESEARCH IN VETERINARY SCIENCE
(2024)
Article
Veterinary Sciences
Zeynep Ozdemir Kutahya, Busra Aslan Akyol, Kubra Deliklitas, Cengiz Gokbulut
Summary: In this study, the pharmacokinetic behavior of Eprinomectin was studied in two different goat breeds. The results showed no significant differences in plasma concentration between the breeds, but the clearance from the body may vary. High concentration of Eprinomectin in feces is beneficial against gastrointestinal parasites but poses an ecotoxicological risk to the environment.
RESEARCH IN VETERINARY SCIENCE
(2024)
Article
Veterinary Sciences
Mohammad Ashrafzadeh-Shiraz, Mousa Tavassoli, Bahram Dalir-Naghadeh, Alireza Sazmand
Summary: This study evaluated the efficacy of fenbendazole and ivermectin on strongyles and Parascaris sp. infecting adult riding horses in different regions of Iran. The results demonstrated varying degrees of resistance of these parasites to both anthelminthics. It emphasizes the urgency of educating horse owners about the importance of a test-and-treatment strategy and suggests evaluating the efficacy of different anthelminthics to choose the most effective treatment in each region.
RESEARCH IN VETERINARY SCIENCE
(2024)
Article
Veterinary Sciences
Moises Gonzalvez, Clara Munoz-Hernandez
Summary: The study evaluated the use of R software in research articles addressing the study of wildlife, showing an increasing trend in the number of publications over time and a wide geographical distribution globally.
RESEARCH IN VETERINARY SCIENCE
(2024)