Review
Cell & Tissue Engineering
Maryam Ghasemi, Elham Roshandel, Mozhdeh Mohammadian, Behrouz Farhadihosseinabadi, Parvin Akbarzadehlaleh, Karim Shamsasenjan
Summary: In recent years, mesenchymal stromal cells (MSCs) have gained scientific attention for their potential in treating incurable diseases. However, concerns about side effects and limitations of direct cell transplantation have led to the exploration of MSC secretome, which consists of bioactive molecules involved in neuro-regeneration. This study reviews the therapeutic effects and mechanisms of MSC secretome in central nervous system disorders, such as Alzheimer's disease, multiple sclerosis, and Parkinson's disease, with the aim of discovering new treatment options.
STEM CELL RESEARCH & THERAPY
(2023)
Review
Cell & Tissue Engineering
Christian Ardianto, Robert Shen, Jimmy F. A. Barus, Poppy Kristina Sasmita, Yuda Turana, Lilis Lilis, Veronika Maria Sidharta
Summary: Parkinson's disease is a common progressive neurodegenerative disease characterized by complex pathological mechanisms. The secretomes released by medicinal signaling cells show potential as a preventive approach by targeting various mechanisms involved in the disease.
REGENERATIVE THERAPY
(2022)
Article
Biochemistry & Molecular Biology
Maria Ageles de Pedro, Maria Pulido, Veronica alvarez, Federica Marinaro, Ana Maria Marchena, Francisco Miguel Sanchez-Margallo, Javier G. Casado, Esther Lopez
Summary: This study found that Menstrual blood-derived stromal cells (MenSCs) have a good survival capacity under acute hypoxia conditions and have potential applications in tissue regeneration through their secretome, making them a potential cell source for wound healing interventions.
MOLECULAR MEDICINE
(2023)
Review
Cell Biology
Rebecca M. Harman, Charlotte Marx, Gerlinde R. van de Walle
Summary: The therapeutic potential of MSC secretome is being intensely studied, but its complexity and variability pose challenges for its application in human medicine. A deeper understanding of the factors that make up the secretome and the ability to manipulate MSCs to consistently secrete factors of biologic importance will improve MSC therapy.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Pharmacology & Pharmacy
Seungwon An, Khandaker Anwar, Mohammadjavad Ashraf, Hyungjo Lee, Rebecca Jung, Raghuram Koganti, Mahmood Ghassemi, Ali R. Djalilian
Summary: In this study, the researchers investigated the use of human bone-marrow-derived MSC secretome (MSC-S) for treating corneal epithelial wounds. They found that MSC extracellular vesicles (EV)/exosomes played a crucial role in mediating the wound-healing effects of MSC-S. Furthermore, they discovered that the concentration and incubation period of MSC-CM had an impact on wound healing.
Review
Biochemistry & Molecular Biology
Georgy Sagaradze, Anna Monakova, Anastasia Efimenko
Summary: Adult stem cells are regulated by their specific microenvironments called stem cell niches, and dysfunction of the niche components can lead to chronic or acute disorders. To address this, regenerative medicine treatments such as gene and cell therapy are being investigated, with a focus on mesenchymal stromal cells (MSCs) and their secretomes. However, the development of MSC secretome-based products is not fully regulated, which has complicated their clinical translation and resulted in failed trials. This review discusses the development of potency assays for MSC secretome-based products, specifically their effects on stem cell niches and spermatogonial stem cell niche.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell & Tissue Engineering
Piotr Poplawski, Weronika Zarychta-Wisniewska, Anna Burdzinska, Joanna Boguslawska, Anna Adamiok-Ostrowska, Karolina Hanusek, Beata Rybicka, Alex Bialas, Helena Kossowska, Roksana Iwanicka-Rokicka, Marta Koblowska, Leszek Paczek, Agnieszka Piekielko-Witkowska
Summary: In this study, it was found that advanced RCC cells secrete AREG, FN1, and HA to induce MSCs migration, and MSCs' gene expression is reprogrammed to facilitate their migration. The identified components of RCC secretome may serve as potential therapeutic targets.
STEM CELL RESEARCH & THERAPY
(2023)
Review
Cell & Tissue Engineering
Ahmed Lotfy, Noha M. AboQuella, Hongjun Wang
Summary: Mesenchymal stromal/stem cells (MSCs) are widely used in cell therapy due to their immunomodulatory and regenerative properties. The production of various factors via extracellular vesicles, especially exosomes, governs the efficacy of MSCs. MSC-derived exosomes (MSC-Exos) show advantages over MSCs themselves by reducing adverse effects. Clinical studies are assessing the therapeutic effect of MSC-Exos in different diseases, and production methods, dosage, and administration routes are being explored.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Biochemistry & Molecular Biology
Elia Bari, Fulvio Tartara, Fabio Cofano, Giuseppe di Perna, Diego Garbossa, Sara Perteghella, Marzio Sorlini, Delia Mandracchia, Lorella Giovannelli, Paolo Gaetani, Maria Luisa Torre, Lorena Segale
Summary: The study found that the mesenchymal stem cell-secretome Lyosecretome has a significant improvement effect on the osteoinductive and osteoconductive properties of titanium cages, leading to increased mineralized matrix when used during culturing.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Niyaz Al-Sharabi, Reinhard Gruber, Mariano Sanz, Samih Mohamed-Ahmed, Einar K. Kristoffersen, Kamal Mustafa, Siddharth Shanbhag
Summary: The secretome of human bone marrow MSC (BMSC) was compared to that of leukocyte- and platelet-rich fibrin (L-PRF), in terms of wound healing and regeneration. The results showed that BMSC secretome had more differentially expressed proteins involved in wound healing, suggesting its potential for periodontal and bone regeneration. Further evaluation of the in vivo efficacy of this therapy is needed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Cell Biology
Trinidad Montero-Vilchez, Alvaro Sierra-Sanchez, Manuel Sanchez-Diaz, Maria Isabel Quinones-Vico, Raquel Sanabria-de-la-Torre, Antonio Martinez-Lopez, Salvador Arias-Santiago
Summary: Mesenchymal stromal cell-conditioned medium (MSC-CM) shows promising potential in treating skin conditions by improving wound healing, hair restoration, skin rejuvenation, and some inflammatory skin diseases. Further research is needed to confirm its safety, effectiveness, and to standardize the manufacturing process.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Immunology
Sanaz Keshavarz Shahbaz, Amir Hossein Mansourabadi, Davood Jafari
Summary: Mesenchymal stem cells (MSCs) have the potential to differentiate into different cell subtypes and have attracted attention in clinical investigations. They can ameliorate immune-related disorders such as graft-versus-host diseases (GVHD) through immunomodulatory effects. Genetic engineering can be used to improve MSC immunosuppressive characteristics and enhance their therapeutic applications.
CLINICAL AND EXPERIMENTAL IMMUNOLOGY
(2022)
Article
Cell & Tissue Engineering
Marwan M. Merkhan, Matthew T. Shephard, Nicholas R. Forsyth
Summary: Modulation of environmental oxygen alters both the concentration and composition of the human mesenchymal stem cell (hMSC) secretome, which is likely to have significant implications for improving understanding and efficacy of hMSC-based therapeutics.
JOURNAL OF TISSUE ENGINEERING
(2021)
Review
Biochemistry & Molecular Biology
Simona-Rebeca Nazarie (Ignat), Sami Gharbia, Anca Hermenean, Sorina Dinescu, Marieta Costache
Summary: Cell therapy with transplanted mesenchymal stem cells (MSCs) can promote liver regeneration, but may pose risks. The secretome of MSCs, including soluble factors and EVs, serves as a safer and more effective cell-free strategy for liver disease treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Cell Biology
Ali Hassanzadeh, Amjad Hussein Altajer, Heshu Sulaiman Rahman, Marwan Mahmood Saleh, Dmitry O. Bokov, Walid Kamal Abdelbasset, Faroogh Marofi, Majid Zamani, Yoda Yaghoubi, Mahboubeh Yazdanifar, Yashwant Pathak, Max Stanley Chartrand, Mostafa Jarahian
Summary: MSC-based therapy in cancer treatment is attracting attention due to their tumor-targeting properties, although controversial, increasing evidence suggests their inhibitory effects on tumor cells. Research focuses on using MSCs as delivery vehicles for chemotherapeutic drugs, immune cell-activating cytokines, and exploring their potential in inducing tumor regression.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Engineering, Biomedical
Tiffany S. Pinho, Deolinda Silva, Jorge Cibrao Ribeiro, Ana Marote, Rui Lima, Salete J. Batista, Rita Melo, Clarisse Ribeiro, Cristiana B. Cunha, Irina S. Moreira, Senentxu Lanceros-Mendez, Antonio J. Salgado
Summary: This study focuses on the application of poled and coated PVDF films for neural differentiation and found that these films can induce neuronal differentiation under mechanical stimulation, which is further enhanced with a pre-coating step of poly-d-lysine and laminin.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2023)
Article
Clinical Neurology
Andreia G. Pinho, Susana Monteiro, Valentina Liberato, Diogo J. Santos, Jonas Campos, Jorge R. Cibra, Nuno A. Silva, Nidia de Sousa, Anton Barreiro-Iglesias, Antonio J. Salgado
Summary: This study aimed to understand the potential of baclofen as a treatment to promote recovery after spinal cord injury (SCI). The results showed that baclofen can improve locomotor and bladder function control in mice after SCI, and it may modulate the immune response. Although further research is needed to explore the mechanisms behind the functional improvements produced by baclofen, this study opens up new avenues for designing pharmacological strategies to promote SCI recovery.
Review
Cell Biology
Joana Sofia Correia, Sara Duarte-Silva, Antonio Jose Salgado, Patricia Maciel
Summary: Spinocerebellar ataxias are heritable neurodegenerative diseases characterized by aggregation of mutant polyglutamine protein in neurons, causing dysfunction and death in the central nervous system. Stem cell transplantation has shown promise in pre-clinical trials for spinocerebellar ataxia types 1, 2, and 3, but the best route of administration and long-term effects are still uncertain. Further studies are needed to optimize cell type selection and delivery strategies, while considering the potential risks of repetitive transplantation. Despite challenges, cell-transplantation therapies have provided transient but beneficial outcomes in spinocerebellar ataxias.
NEURAL REGENERATION RESEARCH
(2023)
Article
Immunology
Wanjing Cen, Felix Umrath, Antonio Jose Salgado, Siegmar Reinert, Dorothea Alexander
Summary: The secretomes derived from osteogenically induced jaw periosteum-derived mesenchymal stem cells (JPCs) have inhibitory effects on the maturation of monocyte-derived dendritic cells (MoDCs), as they reduce the formation of MoDC clusters and down-regulate the expression of CD80, CD86, and CD197 on MoDCs. The secretomes also enhance the uptake capacity of MoDCs and promote the expansion of regulatory T cells. Furthermore, the secretomes affect the gene expression of MoDCs, up-regulating CD209, HLA-DRA, CSF3, IL10, and IL8, and down-regulating IFN gamma and IL12B.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Cell Biology
Joana Martins-Macedo, Antonio Mateus-Pinheiro, Catia Alves, Fernando Veloso, Eduardo D. Gomes, Ines Ribeiro, Joana S. Correia, Tiago Silveira-Rosa, Nuno D. Alves, Ana J. Rodrigues, Joao M. Bessa, Nuno Sousa, Joao F. Oliveira, Patricia Patricio, Luisa Pinto
Summary: This study developed an automated system for exposing rodents to a standard unpredictable chronic mild stress (uCMS) protocol. Comparing manual (uCMS) and automated (auCMS) stress-exposure protocols, the data showed similar impacts on behavioral and physiological measurements. This automated method represents a step forward in preclinical research with advantages in time and standardization.
Article
Multidisciplinary Sciences
Nidia de Sousa, Diego Robledo, Laura Gonzalez-Llera, Andreia G. Pinho, Diogo J. Santos, Susana Monteiro, Jonas Campos, Jorge R. Cibrao, Nuno A. Silva, Laura Sanchez, Antonio J. Salgado, Anton Barreiro-Iglesias
Summary: Spinal cord injury (SCI) has no available pharmacological treatments for promoting neurological recovery. Baclofen treatment has been shown to promote functional recovery in mice with compression SCI. This study provides transcriptomic data from adult mouse spinal cords collected 7 days after compression SCI and baclofen administration. The data includes raw RNA-seq data and bioinformatic analyses.
Article
Engineering, Biomedical
Deolinda Silva, Lucas Schirmer, Tiffany S. Pinho, Passant Atallah, Jorge R. Cibrao, Rui Lima, Joao Afonso, Sandra B-Antunes, Claudia R. Marques, Joao Dourado, Uwe Freudenberg, Rui A. Sousa, Carsten Werner, Antonio J. Salgado
Summary: Adipose tissue-derived stem cells (ASCs) secrete factors that promote regenerative mechanisms, such as axonal growth and inflammation reduction, leading to functional recovery in spinal cord injury (SCI) cases. However, systemic delivery may cause off-target effects, while local administration has low efficiency. This study presents a delivery system based on a hydrogel that continuously releases pro-regenerative cytokines and growth factors, inducing neural differentiation and improving motor function in SCI models.
ADVANCED HEALTHCARE MATERIALS
(2023)
Review
Cell Biology
Rita Silva-Pedrosa, Antonio Jose Salgado, Pedro Eduardo Ferreira
Summary: Cellular models offer opportunities to study human diseases, avoiding ethical restrictions and costs associated with animal models. iPSCs technology overcomes the complexities of hESCs and contributes to diagnosis, therapeutics, and regenerative medicine. 3D cell culture systems, like organoids, provide closer replication of cellular morphology and polarity, enabling the study of organ development, disease modeling, and drug discovery. Although organoids have limitations, they have become a powerful tool for understanding human physiology and the pathogenesis of various diseases.
Article
Biochemistry & Molecular Biology
Rita Caridade-Silva, Bruna Araujo, Joana Martins-Macedo, Fabio G. Teixeira
Summary: Preventing degeneration and the loss of dopaminergic neurons in Parkinson's Disease (PD) remains a challenge, and developing disease-modifying approaches is imperative. N-acetylcysteine (NAC) has shown promising potential in preserving the dopaminergic system and modulating PD mechanisms. In this study, NAC enhanced dopaminergic neuron viability and improved motor deficits in a rat model of PD, suggesting that it may serve as a modulator of PD degenerative mechanisms.
Review
Cell & Tissue Engineering
Bruna Lopes, Andre Coelho, Rui Alvites, Ana Catarina Sousa, Patricia Sousa, Alicia Moreira, Luis Atayde, Antonio Salgado, Stefano Geuna, Ana Colette Mauricio
Summary: Animal models play a crucial role in peripheral nerve injury regeneration studies, but there is a lack of usage of large animal models. The application of large animal models needs to be expanded to improve translational medicine for clinical practice.
REGENERATIVE MEDICINE
(2023)
Article
Cell Biology
Sofia Pereira das Neves, Joao Carlos Sousa, Ricardo Magalhaes, Fuying Gao, Giovanni Coppola, Sebatien Meriaux, Fawzi Boumezbeur, Nuno Sousa, Joao Jose Cerqueira, Fernanda Marques
Summary: Multiple sclerosis is a chronic inflammatory disease of the central nervous system. Recent research has shown that reactive astrocytes in MS lesions undergo metabolic alterations and exhibit increased reactivity, potentially contributing to the progression of the disease.
Article
Cell & Tissue Engineering
Ana Marote, Diogo Santos, Barbara Mendes-Pinheiro, Claudia Serre-Miranda, Sandra Anjo, Joana Vieira, Filipa Ferreira-Antunes, Joana Sofia Correia, Caroline Borges-Pereira, Andreia G. Pinho, Jonas Campos, Bruno Manadas, Manuel R. Teixeira, Margarida Correia-Neves, Luisa Pinto, Pedro M. Costa, Laurent Roybon, Antonio J. Salgado
Summary: This study compared the secretomes of long-term cultured BM-MSCs and iMSCs, and conducted a comprehensive characterization of both sources. The results showed that although there were significant differences in proliferation between the two sources, the secretome composition of both sources was similar in pre-senescent and senescent states. These findings suggest that shifting from BM-MSCs to a more advantageous source like iMSCs may yield similar therapeutic effects as the gold-standard MSC source.
STEM CELL REVIEWS AND REPORTS
(2023)
Meeting Abstract
Cell & Tissue Engineering
Jorge Ribeiro, Rita Silva, Susana Monteiro, Rui Lima, Sofia Serra, Fabio Teixeira, Jose Graca, Nuno Silva, Antonio Salgado
TISSUE ENGINEERING PART A
(2022)
Meeting Abstract
Cell & Tissue Engineering
Deolinda Silva, Passant Atallah, Lucas Schirmer, Ayham Frra, Tiffany Santos Pinho, Antonio Jose Salgado, Carsten Werner
TISSUE ENGINEERING PART A
(2022)
Meeting Abstract
Cell & Tissue Engineering
Sandra Barata-Antunes, Rui A. Sousa, Bruno F. B. Silva, Antonio J. Salgado
TISSUE ENGINEERING PART A
(2022)
