Article
Agriculture, Multidisciplinary
Pingping Zhou, Chunlei Yue, Yuchen Zhang, Yan Li, Xinyi Da, Xiuqi Zhou, Lidan Ye
Summary: This study constructed a rosmarinic acid producing Saccharomyces cerevisiae strain and achieved efficient biosynthesis through the removal of metabolic bottlenecks and tuning down gene expression. It provides a promising platform for fermentative production of rosmarinic acid.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Frederick G. Sunstrum, Hannah L. Liu, Sharon Jancsik, Lufiani L. Madilao, Joerg Bohlmann, Sandra Irmisch
Summary: Montbretins are rare specialized metabolites found in montbretia corms, with Montbretin A (MbA) being of interest for diabetes and obesity treatment. Despite efforts to reconstruct MbA biosynthesis, the major product was therapeutically inactive Montbretin B (MbB). The study identified two functional 4CL enzymes in montbretia, with Cc4CL1 likely involved in MbA biosynthesis and Cc4CL2 possibly contributing to lignin biosynthesis. Both Cc4CLs can be used for flavanone production to support metabolic engineering of MbA in yeast.
Article
Agriculture, Multidisciplinary
Ru Wang, Sangram Keshari Lenka, Varun Kumar, Kelem Gashu, Noga Sikron-Persi, Irena Dynkin, David Weiss, Avichai Perl, Aaron Fait, Michal Oren-Shamir
Summary: The study focuses on enhancing the production of stilbenes, specifically the resveratrol dehydrodimer viniferin, by overexpressing stilbene synthase (STS) and providing ample phenylalanine (Phe). Results show that this strategy significantly increases the accumulation of resveratrol and viniferin in plant cell cultures.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Biotechnology & Applied Microbiology
Mengyao Zhang, Qidou Gao, Yijuan Liu, Zhumei Fang, Zhiwei Gong, Zongbao K. Zhao, Xiaobing Yang
Summary: The study successfully engineered the non-conventional oleaginous yeast R. toruloides for resveratrol production, with an increased yield achieved through protein fusion, co-factor channeling, and optimization of endogenous pathways.
MICROBIAL CELL FACTORIES
(2022)
Article
Plant Sciences
Olga A. Aleynova, Andrey R. Suprun, Nikolay N. Nityagovsky, Alexandra S. Dubrovina, Konstantin V. Kiselev
Summary: This study demonstrates that plant endophytes play a significant role in stimulating stilbene production in grape cell suspension, with endophytic fungi showing a more pronounced induction effect on stilbene accumulation compared to endophytic bacteria. The increase in stilbene content was due to the activation of phenylalanine ammonia lyase (PAL) and stilbene synthase (STS) gene expression. The findings suggest that grape endophytes could potentially serve as stimulators for stilbene biosynthesis in grapevine or grape cell cultures.
Article
Agricultural Engineering
Yanzhe Shang, Ping Zhang, Wenping Wei, Jin Li, Bang-Ce Ye
Summary: By optimizing carbon metabolism, increasing gene copies, and blocking degradation, a high yield of polydatin was successfully produced in Y. lipolytica, demonstrating its great potential for glycoside synthesis.
BIORESOURCE TECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Albert Fina, Stephanie Heux, Joan Albiol, Pau Ferrer
Summary: This study improves the productivity of Pichia pastoris in producing 3-hydroxypropionic acid by combining different metabolic engineering approaches. The results show that, in combination with using glycerol as a carbon source, the metabolically engineered strains achieved the highest concentration and productivity of 3-hydroxypropionic acid reported so far in yeast.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemical Research Methods
Jennifer Perrin, Natalja Kulagina, Marianne Unlubayir, Thibaut Munsch, Ines Carqueijeiro, Thomas Duge de Bernonville, Johan-Owen De Craene, Marc Clastre, Benoit St-Pierre, Nathalie Giglioli-Guivarc'h, David Gagneul, Arnaud Lanoue, Vincent Courdavault, Sebastien Besseau
Summary: THCSpd are plant specialized metabolites with promising pharmacological activities, and can be biosynthesized in yeast using a dual enzymatic system. By exploring enzyme diversity and substrate promiscuities, various new-to-nature THCSpd can be produced, demonstrating a versatile biotechnological platform for tailor-made synthesis.
ACS SYNTHETIC BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Galina N. Veremeichik, Dmitry V. Bulgakov, Yuliya A. Konnova, Evgenia V. Brodovskaya, Valeria P. Grigorchuk, Victor P. Bulgakov
Summary: The overexpression of AtCPK1-Ca form increases pathogenesis-related protein levels in Vitis amurensis cell cultures.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agricultural Engineering
Wen-Yan Tang, Dong-Ping Wang, Yun Tian, Xiao Fan, Chong Wang, Xiang-Yang Lu, Pei-Wang Li, Xiao-Jun Ji, Hu-Hu Liu
Summary: The study successfully increased the production of squalene in Yarrowia lipolytica using pathway engineering and bioprocess engineering, achieving significant experimental results.
BIORESOURCE TECHNOLOGY
(2021)
Review
Biotechnology & Applied Microbiology
Shiyun Li, Qiyue Zhang, Jing Wang, Yingli Liu, Yunying Zhao, Yu Deng
Summary: In order to reduce reliance on petroleum, utilizing industrial microorganisms for the biosynthesis of important chemicals from simple substrates has become a focus of interest. This review explores strategies to increase intracellular malonyl-CoA levels and advances in metabolic engineering for directing malonyl-CoA to desired derivatives, including strengthening supply, reducing consumption, and controlling levels effectively. These strategies offer new insights for enhancing malonyl-CoA derivative synthesis in microorganisms.
JOURNAL OF BIOTECHNOLOGY
(2021)
Review
Food Science & Technology
Aravind Madhavan, K. B. Arun, Deepthy Alex, A. N. Anoopkumar, Shibitha Emmanual, Preeti Chaturvedi, Sunita Varjani, Archana Tiwari, Vinod Kumar, R. Reshmy, Mukesh Kumar Awasthi, Parameswaran Binod, Embalil Mathachan Aneesh, Raveendran Sindhu
Summary: Nutraceuticals have attracted attention for their health-boosting and disease-prevention characteristics. However, limitations in supply and isolation complications have hindered large-scale production. Microbial engineering at the metabolic level offers an environmentally friendly alternative.
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
(2023)
Article
Microbiology
Mei Zhang, Zhu Wang, Zhe Chi, Guang-Lei Liu, Zhen-Ming Chi
Summary: Reducing consumption of acetyl-CoA did not significantly enhance liamocin biosynthesis by engineered strains of A. melanogenm, but instead decreased cell growth. Careful selection of genes for expression is crucial to avoid negative impacts on liamocin production.
MICROBIOLOGICAL RESEARCH
(2022)
Article
Plant Sciences
Viviana Martins, Marianne Unlubayir, Antonio Teixeira, Hernani Geros, Arnaud Lanoue
Summary: The study suggests a potential interaction between Ca and MeJA signaling pathways in regulating specific secondary metabolism routes in grape cells. The combination of MeJA+Ca specifically enhances the accumulation of Eresveratrol and E-e-viniferin, while inhibiting the stimulatory effect of MeJA on flavonoid synthesis by down-regulating most flavonoid pathway genes. Ca can mimic or potentiate the effect of MeJA on the expression of JA signaling genes.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Article
Endocrinology & Metabolism
Alexander Chou, Seung Hwan Lee, Fayin Zhu, James M. Clomburg, Ramon Gonzalez
Summary: The study introduces an alternative approach using synthetic pathways for C1 bioconversion, generating multicarbon products directly from formate, formaldehyde and methanol while being orthogonal to the host metabolic network. Variants of the FORCE pathways were evaluated through thermodynamic and stoichiometric analyses, showing promising results both in vitro and in vivo. This synthetic metabolic pathway demonstrates the potential for C1 compound utilization and integration with host metabolism for synthetic methylotrophy.
Article
Microbiology
Larissa Balabanova, Aleksandra Seitkalieva, Yulia Yugay, Tatiana Rusapetova, Lubov Slepchenko, Anna Podvolotskaya, Margarita Yatsunskaya, Elena Vasyutkina, Oksana Son, Liudmila Tekutyeva, Yury Shkryl
Summary: A study was conducted to enhance the nutritional value of mycoprotein by overexpressing plant storage proteins in a fungus using an efficient genetic transformation approach, resulting in increased levels of valuable nutritional proteins and improved amino acid content in the fungal biomass.
Article
Plant Sciences
Yury Shkryl, Galina Veremeichik, Tatiana Avramenko, Tatiana Gorpenchenko, Galina Tchernoded, Victor Bulgakov
Summary: The overexpression of the rolC gene from Agrobacterium rhizogenes suppresses the expression of oxidative and antioxidative enzymes, reduces the generation of reactive oxygen species (ROS), and enhances stress tolerance in transgenic cells.
PLANT GROWTH REGULATION
(2022)
Article
Plant Sciences
Galina N. Veremeichik, Yuri N. Shkryl, Tatiana Rusapetova, Slavena A. Silantieva, Valeria P. Grigorchuk, Petr V. Velansky, Evgenia Brodovskaya, Yuliya A. Konnova, Anastasia A. Khopta, Dmitry Bulgakov, Victor P. Bulgakov
Summary: The overexpression of A4-rolB gene can increase flavonol accumulation and enhance drought tolerance in plants, which can be explained by the cooperative action of SA and ROS pathways.
Article
Biochemistry & Molecular Biology
Yulia V. Vereshchagina, Anastasiya A. Mironova, Dmitry V. Bulgakov, Victor P. Bulgakov
Summary: The rolB gene transferred from Agrobacterium rhizogenes to plants can be stably inherited in the plant offspring. Proteomic analysis of rolB-expressing Arabidopsis thaliana plants revealed both upregulated and downregulated proteins, some of which are associated with plant defense systems and interaction with pathogens. These findings contribute to our understanding of the impact of the rolB gene on plants and further research on plant defense systems.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biotechnology & Applied Microbiology
Galina N. Veremeichik, Dmitrii V. Bulgakov, Taisia O. Solomatina, Dmitrii S. Makhazen
Summary: Compared to other components of horizontal gene transfer in agrobacterial colonization of plants, the biological function of the agrobacterial oncogene rolA is poorly understood. Available information on this problem has been reviewed in this paper, but other rol oncogenes have been studied more extensively. Despite limited data, it is suggested that the rolA oncogene and its regulatory apparatus have great potential in plant biotechnology and genetic engineering.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Chemistry, Applied
G. N. Veremeichik, V. P. Grigorchuk, D. S. Makhazen, E. P. Subbotin, A. S. Kholin, N. I. Subbotina, D. V. Bulgakov, Y. N. Kulchin, V. P. Bulgakov
Summary: In this study, it was found that red/blue light resulted in a 20-fold increase in flavonol productivity in arugula plants compared to white light. However, it had no effect on anthocyanin productivity. On the other hand, red/blue light increased the anthocyanin content and productivity of arugula plants more than ten times compared to white light. Overall, this research demonstrates that flavonoid content can be significantly increased without a loss in biomass.
Article
Biology
Tatiana Y. Gorpenchenko, Galina N. Veremeichik, Yurii N. Shkryl, Yulia A. Yugay, Valeria P. Grigorchuk, Dmitry V. Bulgakov, Tatiana V. Rusapetova, Yulia V. Vereshchagina, Anastasiya A. Mironova, Evgeniyy P. Subbotin, Yuriy N. Kulchin, Victor P. Bulgakov
Summary: HOS1 gene is an important regulator of plant cold tolerance and developmental processes. Silencing HOS1 gene increases cold tolerance and moderately increases ROS levels under low and high light conditions. HOS1 is required for maintaining ROS homeostasis under cold conditions and prevents overinduction of defense mechanisms.
Article
Biochemistry & Molecular Biology
Yury N. N. Shkryl, Galina K. K. Tchernoded, Yulia A. A. Yugay, Valeria P. P. Grigorchuk, Maria R. R. Sorokina, Tatiana Y. Y. Gorpenchenko, Olesya D. D. Kudinova, Anton I. I. Degtyarenko, Maria S. S. Onishchenko, Nikita A. A. Shved, Vadim V. V. Kumeiko, Victor P. P. Bulgakov
Summary: Aristolochia manshuriensis is a rare plant used in traditional Chinese medicine and endemic to a specific region. By establishing hairy root cultures of this plant with modified genes, we were able to significantly increase the production of valuable compounds while reducing toxic components. The extracts from these hairy roots showed strong cytotoxicity against certain cancer cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Galina N. Veremeichik, Dmitry V. Bulgakov, Yuliya A. Konnova, Evgenia V. Brodovskaya, Valeria P. Grigorchuk, Victor P. Bulgakov
Summary: The overexpression of AtCPK1-Ca form increases pathogenesis-related protein levels in Vitis amurensis cell cultures.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Galina N. Veremeichik, Tatiana Y. Gorpenchenko, Tatiana V. Rusapetova, Evgenia V. Brodovskaya, Galina K. Tchernoded, Dmitry V. Bulgakov, Yurii N. Shkryl, Victor P. Bulgakov
Summary: This study investigated the stable pRiA4-transformed callus culture of Rubia cordifolia L. and found that the transformed tissue can achieve long-term cultivation without the need for plant growth regulators (PGRs). Moreover, the transformed tissue exhibited higher productivity of anthraquinone (AQ) compared to untransformed tissue. These findings have important implications in selecting optimal PGR combinations for the cultivation of rare, medicinal, and woody plants, and highlight the potential application of genetic modification in plant biotechnology.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Review
Agronomy
David Adedayo Animasaun, Peter Adeolu Adedibu, Yury Shkryl, Faith Olatayo Emmanuel, Liudmila Tekutyeva, Larissa Balabanova
Summary: Food insecurity is a pressing global issue exacerbated by climate change, conflicts, pandemics, and economic recessions. Genetic engineering of crops offers potential solutions through the development of pest and disease-resistant varieties, increased yield, and edible vaccines. However, government policies in many countries hinder the acceptance and distribution of this technology.
Article
Plant Sciences
Yulia Yugay, Zhargalma Tsydeneshieva, Tatiana Rusapetova, Olga Grischenko, Anastasia Mironova, Dmitry Bulgakov, Vladimir Silant'ev, Galina Tchernoded, Victor Bulgakov, Yury Shkryl
Summary: EVs have been isolated and characterized from callus culture of Arabidopsis thaliana, showing similarities with intact plants in terms of protein and microRNA composition.
Article
Engineering, Biomedical
Yulia A. Yugay, Maria R. Sorokina, Valeria P. Grigorchuk, Tatiana V. Rusapetova, Vladimir E. Silant'ev, Anna E. Egorova, Peter A. Adedibu, Olesya D. Kudinova, Elena A. Vasyutkina, Vladimir V. Ivanov, Alexander A. Karabtsov, Dmitriy V. Mashtalyar, Anton I. Degtyarenko, Olga V. Grishchenko, Vadim V. Kumeiko, Victor P. Bulgakov, Yury N. Shkryl
Summary: This study explores a novel method for synthesizing extracellular silver nanoparticles using Aristolochia manshuriensis cultured cells. The nanoparticles exhibited spherical morphology and were able to inhibit the growth of certain cells and pathogenic microorganisms.
JOURNAL OF FUNCTIONAL BIOMATERIALS
(2023)