期刊
PROCESS BIOCHEMISTRY
卷 59, 期 -, 页码 180-186出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.procbio.2017.05.008
关键词
Camellia sinensis; Polyphenol oxidase; Recombinant; Protein refolding
资金
- CSIR network project SIMPLE [BSC 0109]
- CSIR, New Delhi, India
Polyphenol oxidases (PPOs) are copper-containing industrially important enzymes that catalyze the synthesis of many commercially important products by using polyphenols as substrate. Camellia sinensis polyphenol oxidase (CsPPO) is interesting because it oxidizes epicatechins to yield theaflavins and thearubigins. The present study aimed to optimize the expression of CsPPO in Escherichia coll. Because CsPPO had a large number of E. coli rare codons, it yielded a poor quantity of protein in E. colt Rosetta (TM) 2 cells, which have additional tRNAs for E. coli rare codons. Thus, synthetically constructed codon-optimized CsPPO was cloned into pET-47b(+) vector and expressed in a bacterial host. Ectopic expression led to the formation of inclusion bodies. However, extensive standardization of buffers and methods of refolding such as dialysis, on-column refolding, and rapid dilution yielded active PPO from solubilized inclusion bodies with copper content of 0.880 +/- 0.095 atom/molecule of protein. Experimental data produced maximum PPO activity in a rapid dilution buffer containing 0.5 M L-arginine. Refolded CsPPO liad an optimum pH of 5.0 and K-m values of 3.10, 0.479, and 0.314 mM, and a V-max of 163.9, 82.64, and 142.8 U/mg of protein for catechol, catechin, and epicatechin, respectively.
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