Article
Multidisciplinary Sciences
Sayaka Wakayama, Daiyu Ito, Yuko Kamada, Toru Shimazu, Tomomi Suzuki, Aiko Nagamatsu, Ryoko Araki, Takahiro Ishikawa, Satoshi Kamimura, Naoki Hirose, Kousuke Kazama, Li Yang, Rei Inoue, Yasuyuki Kikuchi, Erika Hayashi, Rina Emura, Ren Watanabe, Hiroaki Nagatomo, Hiromi Suzuki, Tohru Yamamori, Motoki N. Tada, Ikuko Osada, Masumi Umehara, Hiromi Sano, Haruo Kasahara, Akira Higashibata, Sachiko Yano, Masumi Abe, Satoshi Kishigami, Takashi Kohda, Masatoshi Ooga, Teruhiko Wakayama
Summary: The experiment of preserving freeze-dried mouse spermatozoa on the International Space Station (ISS) for the longest period showed that space radiation did not affect sperm DNA or fertility, producing genetically normal offspring at a success rate comparable to ground-preserved controls. These results suggest that the impact of deep space radiation on mammalian reproduction can be evaluated using spermatozoa, even without astronauts monitoring in Gateway.
Article
Biophysics
Monica Guarro, Francisca Suner, Marti Lecina, Salvador Borros, Cristina Fornaguera
Summary: This study describes an alternative and easy-to-be-applied methodology for the lyophilization of extracellular vesicles (EVs). By using sucrose as lyoprotectant, the cryopreservation efficiency was improved, and the properties and functionality of freeze-dried EVs were confirmed through various tests.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2022)
Article
Biochemistry & Molecular Biology
Nathalie Le Foll, Jean-Christophe Pont, Audrey L'Hostis, Thomas Guilbert, Frederic Bouillaud, Jean-Philippe Wolf, Ahmed Ziyyat
Summary: cFEE improves gamete interaction in humans by enhancing sperm movement parameters and increasing the percentage of hyperactivated sperm. However, it does not significantly impact the analyzed mitochondrial bioenergetic parameters, indicating that it may act through a more efficient use of energy resources.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Sayaka Wakayama, Mariko Soejima, Yasuyuki Kikuchi, Erika Hayashi, Natsuki Ushigome, Ayumi Hasegawa, Keiji Mochida, Tomomi Suzuki, Chiaki Yamazaki, Toru Shimazu, Hiromi Sano, Masumi Umehara, Hitomi Matsunari, Atsuo Ogura, Hiroshi Nagashima, Teruhiko Wakayama
Summary: This paper describes the development of a new device that allows astronauts to thaw and culture frozen mouse embryos on the ISS without direct contact. The device utilizes different methods to address the issue of using liquid nitrogen on the ISS. The application of this device is significant for astronauts in the thawing and culturing of embryos, as well as in the fields of clinical infertility and animal biotechnology.
Article
Health Care Sciences & Services
Ioana Zaha, Petronela Naghi, Liana Stefan, Cosmina Bunescu, Mihaela Radu, Mariana Eugenia Muresan, Mircea Sandor, Liliana Sachelarie, Anca Huniadi
Summary: The microfluidic sperm selection method was found to improve the outcomes of in vitro fertilization (IVF) procedures. This method, which selects spermatozoa with the lowest DNA fragmentation rate, resulted in higher blastocyst rates and clinical pregnancy rates. It has the potential to be widely used in intracytoplasmic sperm injection (ICSI) and improve the workflow of standard IVF, reducing intervention by laboratory personnel and providing more consistent incubation conditions.
JOURNAL OF PERSONALIZED MEDICINE
(2023)
Article
Microbiology
Rachita K. Kumar, Nitin Kumar Singh, Sanjaay Balakrishnan, Ceth W. Parker, Karthik Raman, Kasthuri Venkateswaran
Summary: This study predicts possible metabolic interactions in the ISS microbiome using a computational approach and sheds further light on its organization. The results show beneficial interactions between Klebsiella pneumoniae and Pantoea genus, and parasitic and amensalistic interactions between Klebsiella pneumoniae and Aspergillus and Penicillium species. Klebsiella pneumoniae has positive effects on the survival of other microorganisms, but may also have negative interactions with potential pathogens.
Article
Microbiology
Anna C. Simpson, V. V. Ramprasad Eedara, Nitin K. Singh, Namita Damle, Ceth W. Parker, Fathi Karouia, Christopher E. Mason, Kasthuri Venkateswaran
Summary: In April 2018, a strain called Cohnella hashimotonis was isolated from the wall of the Waste Hygiene Compartment on the International Space Station (ISS). This bacterium belongs to the family Paenibacillaceae and exhibits gram-positive, rod-shaped characteristics, as well as motility and the ability to metabolize various carbon compounds.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Adriana Blachowicz, Jillian Romsdahl, Abby J. Chiang, Sawyer Masonjones, Markus Kalkum, Jason E. Stajich, Tamas Torok, Clay C. C. Wang, Kasthuri Venkateswaran
Summary: This study reports the genomic, proteomic, and metabolomic alterations in Aspergillus niger strain JSC-093350089 grown in a controlled experiment aboard the International Space Station (ISS). Results show that microgravity and enhanced irradiation triggered non-synonymous point mutations in specific regions of the genome, altered protein abundance, and changed the metabolite composition.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Swati Bijlani, Nitin K. Singh, V. V. Ramprasad Eedara, Appa Rao Podile, Christopher E. Mason, Clay C. C. Wang, Kasthuri Venkateswaran
Summary: Three novel bacterial strains, IF7SW-B2(T), IIF1SW-B5, and IIF4SW-B5, belonging to the genus Methylobacterium, were isolated from the International Space Station (ISS). These strains are proposed to be a new species named Methylobacterium ajmalii based on genomic, phylogenetic, biochemical, and fatty acid analyses.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Charles S. Cockell, Rosa Santomartino, Kai Finster, Annemiek C. Waajen, Natasha Nicholson, Claire-Marie Loudon, Lorna J. Eades, Ralf Moeller, Petra Rettberg, Felix M. Fuchs, Rob Van Houdt, Natalie Leys, Ilse Coninx, Jason Hatton, Luca Parmitano, Jutta Krause, Andrea Koehler, Nicol Caplin, Lobke Zuijderduijn, Alessandro Mariani, Stefano Pellari, Fabrizio Carubia, Giacomo Luciani, Michele Balsamo, Valfredo Zolesi, Jon Ochoa, Pia Sen, James A. J. Watt, Jeannine Doswald-Winkler, Magdalena Herova, Bernd Rattenbacher, Jennifer Wadsworth, R. Craig Everroad, Rene Demets
Summary: The study found that under different gravity conditions, Sphingomonas desiccabilis and Bacillus subtilis enhanced the leaching of vanadium by 184.92% to 283.22% compared to sterile controls. Gravity did not significantly affect the mean leaching rate, demonstrating the potential for biomining on Solar System objects.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Thermodynamics
A. Sielaff, D. Mangini, O. Kabov, M. Q. Raza, A. I. Garivalis, M. Zupancic, S. Dehaeck, S. Evgenidis, C. Jacobs, D. Van Hoof, O. Oikonomidou, X. Zabulis, P. Karamaounas, A. Bender, F. Ronshin, M. Schinnerl, J. Sebilleau, C. Colin, P. Di Marco, T. Karapantsios, I. Golobic, A. Rednikov, P. Colinet, P. Stephan, L. Tadrist
Summary: This publication lays the foundation for the description of the Multiscale Boiling Experiment conducted on the International Space Station. The experiment investigates two-phase heat transfer during boiling processes by selectively igniting bubbles on a heated substrate using a short laser pulse. The study is significant for understanding the phenomena and has generated over 3000 datasets over an 11-month measurement period.
APPLIED THERMAL ENGINEERING
(2022)
Article
Reproductive Biology
Guo Chen, Li Ren, Zhanglin Chang, Yuting Zhao, Yanwen Zhang, Dong Xia, Ruqian Zhao, Bin He
Summary: High glucose concentration-induced lysine acetylation participates in the regulation of boar spermatozoa motility and acrosome integrity during preservation.
Article
Engineering, Aerospace
Adrian Blagau, Andrei Ersen, Corina Dobrescu, Octav Marghitu
Summary: This paper investigates the magnetic field data provided by the Astro Pi sensor onboard the International Space Station (ISS) and finds good agreement with the geomagnetic field model. The paper points out the difficulty of extracting the magnetic field contributed by ISS due to the ISS not being designed for magnetic field observations, but proposes an optimization procedure to overcome this issue. However, the accuracy of the results is currently limited by sensor performance and lack of attitude information, but can be improved by better sensors and data processing algorithms. The paper highlights the importance of the ISS in providing magnetic field data at low altitudes rarely reached by satellites for monitoring and modeling purposes.
Article
Engineering, Aerospace
Alina Kunitskaya, James M. Piret, Nicole Buckley, Etienne Low-Decarie
Summary: Despite extensive research, the understanding of human health changes resulting from long-duration space flight is still limited. This study provides a compilation of data from 37 health studies, analyzing 517 parameters, to enhance our understanding of the effects of long-duration spaceflight on human health. The results suggest that there are significant physiological and biochemical changes during spaceflight, while the changes post-flight are minimal.
Article
Microbiology
Camilla Urbaniak, Michael D. Morrison, James B. Thissen, Fathi Karouia, David J. Smith, Satish Mehta, Crystal Jaing, Kasthuri Venkateswaran
Summary: This study provides a comprehensive analysis of the International Space Station (ISS) surface microbiome, including microbial burden, prevalence of bacterial and fungal species, temporal and spatial changes in the microbiome and resistome, as well as the functional capabilities and microbial interactions of this unique built microbiome. The results show that the ISS surface microbiome is dominated by organisms associated with the human skin. The study also identifies antimicrobial resistance genes and assesses the functional potential of the microbiome.
Article
Plant Sciences
Akie Kobayashi, Hye-Jeong Kim, Yuta Tomita, Yutaka Miyazawa, Nobuharu Fujii, Sachiko Yano, Chiaki Yamazaki, Motoshi Kamada, Haruo Kasahara, Sachiko Miyabayashi, Toru Shimazu, Yasuo Fusejima, Hideyuki Takahashi
PHYSIOLOGIA PLANTARUM
(2019)
Article
Microbiology
Hideki Yamamura, Takuma Hayashi, Moriyuki Hamada, Takashi Kohda, Yukihiko Serisawa, Kazuyo Matsuyama-Serisawa, Youji Nakagawa, Misa Otoguro, Fujitoshi Yanagida, Tomohiko Tamura, Masayuki Hayakawa
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2019)
Article
Endocrinology & Metabolism
Mika Ikegame, Atsuhiko Hattori, Makoto J. Tabata, Kei-ichiro Kitamura, Yoshiaki Tabuchi, Yukihiro Furusawa, Yusuke Maruyama, Tatsuki Yamamoto, Toshio Sekiguchi, Risa Matsuoka, Taizo Hanmoto, Takahiro Ikari, Masato Endo, Katsunori Omori, Masaki Nakano, Sayaka Yashima, Sadakazu Ejiri, Toshiki Taya, Hiroshi Nakashima, Nobuaki Shimizu, Masahisa Nakamura, Takashi Kondo, Kazuichi Hayakawa, Ichiro Takasaki, Atsushi Kaminishi, Ryosuke Akatsuka, Yuichi Sasayama, Takumi Nishiuchi, Masayuki Nara, Hachiro Iseki, Vishwajit S. Chowdhury, Shigehito Wada, Kenichi Ijiri, Toshio Takeuchi, Tohru Suzuki, Hironori Ando, Kouhei Matsuda, Masanori Somei, Hiroyuki Mishima, Yuko Mikuni-Takagaki, Hisayuki Funahashi, Akihisa Takahashi, Yoshinari Watanabe, Masahiro Maeda, Hideaki Uchida, Akio Hayashi, Akira Kambegawa, Azusa Seki, Sachiko Yano, Toru Shimazu, Hiromi Suzuki, Jun Hirayama, Nobuo Suzuki
JOURNAL OF PINEAL RESEARCH
(2019)
Article
Multidisciplinary Sciences
Shogo Matoba, Shoko Nakamuta, Kento Miura, Michiko Hirose, Hirosuke Shiura, Takashi Kohda, Nobuaki Nakamuta, Atsuo Ogura
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2019)
Article
Astronomy & Astrophysics
Motoshi Kamada, Mariko Oka, Riko Inoue, Yayoi Fujitaka, Kensuke Miyamoto, Eiji Uheda, Chiaki Yamazaki, Toru Shimazu, Hiromi Sano, Haruo Kasahara, Tomomi Suzuki, Akira Higashibata, Junichi Ueda
LIFE SCIENCES IN SPACE RESEARCH
(2019)
Article
Biochemistry & Molecular Biology
Yuzuru Kato, Tokuko Iwamori, Youichirou Ninomiya, Takashi Kohda, Jyunko Miyashita, Mikiko Sato, Yumiko Saga
Article
Multidisciplinary Sciences
Daiji Kiyozumi, Taichi Noda, Ryo Yamaguchi, Tomohiro Tobita, Takafumi Matsumura, Kentaro Shimada, Mayo Kodani, Takashi Kohda, Yoshitaka Fujihara, Manabu Ozawa, Zhifeng Yu, Gabriella Miklossy, Kurt M. Bohren, Masato Horie, Masaru Okabe, Martin M. Matzuk, Masahito Ikawa
Article
Multidisciplinary Sciences
Joongoo Lee, Kevin J. Schwarz, Do Soon Kim, Jeffrey S. Moore, Michael C. Jewett
NATURE COMMUNICATIONS
(2020)
Article
Multidisciplinary Sciences
Kenichi Kawahori, Yoshitaka Kondo, Xunmei Yuan, Yuki Kawasaki, Nozomi Hanzawa, Kazutaka Tsujimoto, Fumiko Wada, Takashi Kohda, Akihito Ishigami, Tetsuya Yamada, Yoshihiro Ogawa, Koshi Hashimoto
SCIENTIFIC REPORTS
(2020)
Article
Multidisciplinary Sciences
Sayaka Wakayama, Daiyu Ito, Yuko Kamada, Toru Shimazu, Tomomi Suzuki, Aiko Nagamatsu, Ryoko Araki, Takahiro Ishikawa, Satoshi Kamimura, Naoki Hirose, Kousuke Kazama, Li Yang, Rei Inoue, Yasuyuki Kikuchi, Erika Hayashi, Rina Emura, Ren Watanabe, Hiroaki Nagatomo, Hiromi Suzuki, Tohru Yamamori, Motoki N. Tada, Ikuko Osada, Masumi Umehara, Hiromi Sano, Haruo Kasahara, Akira Higashibata, Sachiko Yano, Masumi Abe, Satoshi Kishigami, Takashi Kohda, Masatoshi Ooga, Teruhiko Wakayama
Summary: The experiment of preserving freeze-dried mouse spermatozoa on the International Space Station (ISS) for the longest period showed that space radiation did not affect sperm DNA or fertility, producing genetically normal offspring at a success rate comparable to ground-preserved controls. These results suggest that the impact of deep space radiation on mammalian reproduction can be evaluated using spermatozoa, even without astronauts monitoring in Gateway.
Article
Microscopy
Ryohei Yamaura, Daisuke Tamaoki, Hiroyuki Kamachi, Daisuke Yamauchi, Yoshinobu Mineyuki, Kentaro Uesugi, Masato Hoshino, Tomomi Suzuki, Toru Shimazu, Haruo Kasahara, Motoshi Kamada, Yuko T. Hanba, Atsushi Kume, Tomomichi Fujita, Ichirou Karahara
Summary: This study developed a method to visualize and quantitatively analyze the rhizoid system of the moss Physcomitrium patens, using X-ray micro-computed tomography and machine learning algorithms. The research successfully achieved the three-dimensional visualization of the moss rhizoid system.
Article
Astronomy & Astrophysics
Chiaki Yamazaki, Tomokazu Yamazaki, Mikiko Kojima, Yumiko Takebayashi, Hitoshi Sakakibara, Eiji Uheda, Mariko Oka, Motoshi Kamada, Toru Shimazu, Haruo Kasahara, Hiromi Sano, Tomomi Suzuki, Akira Higashibata, Kensuke Miyamoto, Junichi Ueda
Summary: The functional relationships between plant hormones and the growth and development of shoots in etiolated Alaska pea and etiolated Golden Cross Bantam maize seedlings under different gravities were investigated. Comprehensive analyses of 31 species of plant hormones were conducted, revealing the positive correlation between endogenous levels of auxin and bending and length of epicotyls in pea seedlings grown under microgravity conditions. On the other hand, there was almost no significant relationship between endogenous plant hormone levels and growth and development in etiolated maize seedlings grown under microgravity conditions, except for higher levels of IAA compared to Earth-grown seedlings.
LIFE SCIENCES IN SPACE RESEARCH
(2023)
Article
Developmental Biology
Hirosuke Shiura, Ryuichi Ono, Saori Tachibana, Takashi Kohda, Tomoko Kaneko-Ishino, Fumitoshi Ishino
Summary: The Peg10 gene plays a crucial role in placental formation and maintenance. The PEG10 protein shares similarity with LTR retrotransposon GAG and POL proteins, with the viral aspartic protease motif in the POL-like region being essential for retrotransposon/retrovirus life cycle. The interaction between Peg10 and Rtl1 genes is essential for the maintenance of fetal capillary endothelial cells along the feto-maternal placenta interface.
Article
Biochemistry & Molecular Biology
Mariko Mizuguchi, Toshifumi Hara, Manami Yoshita-Takahashi, Takashi Kohda, Yuetsu Tanaka, Masataka Nakamura
Summary: The study reveals that DNA methylation in T-cells affects the binding of KLF2 to hTERT, thereby regulating the expression of the hTERT gene.
BIOCHEMISTRY AND BIOPHYSICS REPORTS
(2021)
Article
Astronomy & Astrophysics
Kensuke Miyamoto, Akinori Inui, Eiji Uheda, Mariko Oka, Motoshi Kamada, Chiaki Yamazaki, Toru Shimazu, Haruo Kasahara, Hiromi Sano, Tomomi Suzuki, Akira Higashibata, Junichi Ueda
LIFE SCIENCES IN SPACE RESEARCH
(2019)