期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 114, 期 28, 页码 E5741-E5749出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1706711114
关键词
actin; cytoskeleton; Golgi; image processing; networks
资金
- International Max Planck Research School scholarship
- Max Planck Society
- Australian Research Council [FT160100218]
- R@MAP Professorship at University of Melbourne
- Dyason travel grant
- Australian Research Council [FT160100218] Funding Source: Australian Research Council
The actin cytoskeleton is an essential intracellular filamentous structure that underpins cellular transport and cytoplasmic streaming in plant cells. However, the system-level properties of actin-based cellular trafficking remain tenuous, largely due to the inability to quantify key features of the actin cytoskeleton. Here, we developed an automated image-based, network-driven framework to accurately segment and quantify actin cytoskeletal structures and Golgi transport. We show that the actin cytoskeleton in both growing and elongated hypocotyl cells has structural properties facilitating efficient transport. Our findings suggest that the erratic movement of Golgi is a stable cellular phenomenon that might optimize distribution efficiency of cell material. Moreover, we demonstrate that Golgi transport in hypocotyl cells can be accurately predicted from the actin network topology alone. Thus, our framework provides quantitative evidence for system-wide coordination of cellular transport in plant cells and can be readily applied to investigate cytoskeletal organization and transport in other organisms.
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