Review
Cell Biology
I. -Ying Kuo, Chih-Hsiung Hsieh, Wan-Ting Kuo, Chih-Peng Chang, Yi-Ching Wang
Summary: Cells in the tumor microenvironment require a class of checkpoints to regulate various secretion and transport processes. Recent studies have shown that Rab proteins mediate communication between diverse cargoes and substrates through vesicular trafficking and secretion pathways in the tumor microenvironment. This review focuses on the role and regulation of vesicular secretion pathways in the tumor microenvironment, and describes the latest research advances and future directions.
JOURNAL OF BIOMEDICAL SCIENCE
(2022)
Review
Biochemistry & Molecular Biology
Alazne Arrazola Sastre, Miriam Luque Montoro, Hadriano M. Lacerda, Francisco Llavero, Jose L. Zugaza
Summary: Rab and Arf GTPases play crucial roles in the pathogenesis and progression of neurodegenerative diseases, making them potential therapeutic targets.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Medicine, Research & Experimental
Jiayi Liu, Xuanjun Zheng, Xiaoqian Wu
Summary: This review summarizes the importance of Rab GTPase in cardiovascular diseases and suggests its potential as a therapeutic target.
Review
Biochemistry & Molecular Biology
Qingchun Lu, Po-Shun Wang, Ling Yang
Summary: This review focuses on highlighting Golgi-associated Rab GTPases as master regulators of autophagic flux, discussing the future research prospects in elucidating their role and mechanism in autophagy and autophagy-related diseases.
CELL AND BIOSCIENCE
(2021)
Review
Cell Biology
Klaus Ebnet, Volker Gerke
Summary: Membrane polarity, regulated by polarized membrane trafficking and the presence of intramembrane diffusion barriers, is crucial for the development of multicellular tissues. Rho and Rab family small GTPases play important roles in cell-cell junction formation and polarized membrane traffic.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Oncology
Jeong-In Park, Kyung-Hee Song, Seong-Mook Kang, Jeeyong Lee, Seong-Jun Cho, Hyun Kyung Choi, Jiyeon Ahn, Jong-Kuk Park, Jaesung Kim, Sang-Gu Hwang, Dae-Seog Lim, Joon Kim, Seung-Youn Jung, Jie-Young Song
Summary: This study demonstrates that BHMPS inhibits migration and invasion of breast cancer cells by blocking the interaction between Rab27a and Slp4, interfering with vesicle trafficking and secretion. BHMPS also decreases the expression of epithelial-mesenchymal transition transcription factors by inhibiting FAK and JNK activation, and reduces the secretion of epidermal growth factor receptor and fibronectin. In addition, BHMPS suppresses tumor growth in Rab27a-overexpressing MDA-MB-231 xenograft mice.
Article
Biochemistry & Molecular Biology
Fumitaka Kawakami, Motoki Imai, Yuki Isaka, Mark R. Cookson, Hiroko Maruyama, Makoto Kubo, Matthew J. Farrer, Makoto Kanzaki, Rei Kawashima, Tatsunori Maekawa, Shun Tamaki, Yoshifumi Kurosaki, Fumiaki Kojima, Kenichi Ohba, Takafumi Ichikawa
Summary: This study demonstrates the negative impact of LRRK2 on glucose tolerance in high-fat diet-induced mice. In adipose tissue, LRRK2 kinase activity suppresses Rab8 and Rab10-mediated GLUT4 membrane translocation, exacerbating glucose intolerance.
Article
Cell Biology
Junru Feng, Hui Lu, Wenhao Ma, Wenjing Tian, Zhuan Lu, Hongying Yang, Yongping Cai, Pengfei Cai, Yuchen Sun, Zilong Zhou, Jiaqian Feng, Jiazhong Deng, Ying Shu, Kun Qu, Weidong Jia, Ping Gao, Huafeng Zhang
Summary: Metformin is a potential anti-tumor agent in hepatocellular carcinoma (HCC), but its effectiveness varies among patients. This study reveals that levels of DOCK1, a protein, determine the response to metformin treatment. The combination of metformin with a DOCK1 inhibitor shows promising results in enhancing anti-tumor activity in HCC, particularly in patients with low DOCK1 expression.
Article
Plant Sciences
Fan-Chen Huang, Shin-Fei Chi, Pei-Ru Chien, Yin-Tzu Liu, Hsin-Nung Chang, Choun-Sea Lin, Hau-Hsuan Hwang
Summary: AtRAB8s interact with AtRTNLB proteins and are involved in Agrobacterium tumefaciens and Pseudomonas syringae infection processes.
PLANT AND CELL PHYSIOLOGY
(2021)
Article
Cell Biology
Sarah D. Neuman, Annika R. Lee, Jane E. Selegue, Amy T. Cavanagh, Arash Bashirullah
Summary: Regulated exocytosis is a crucial process where specific cargo proteins are secreted in response to stimuli. The study utilized Drosophila larval salivary glands to identify a new role for Rab GTPases in granule maturation, revealing that Rab11 recruits Rab1 to drive granule growth and both GTPases have additional functions during exocytosis.
JOURNAL OF CELL SCIENCE
(2021)
Article
Cell Biology
Suting Li, Zhi Wang, Mao Chen, Ya Xiao, Jie Min, Ming Hu, Jianming Tang, Li Hong
Summary: In this study, ArfGAP3 was found to be expressed during skeletal muscle injury and myoblast differentiation, and positively correlated with COPI-related genes. In vitro experiments showed that ArfGAP3 regulates COPI-associated vesicle and GLUT4 transport, impacting myoblast proliferation and differentiation by influencing glucose uptake. These findings provide insight into the repair process after skeletal muscle injury.
CELLULAR SIGNALLING
(2023)
Article
Multidisciplinary Sciences
Xin Yong, Guowen Jia, Zhe Liu, Chunzhuang Zhou, Jiamin Yi, Li Chen, Lu Chen, Yuan Wang, Qingxiang Sun, Daniel D. Billadeau, Zhaoming Su, Da Jia
Summary: In this study, the structure of the Drosophila Mon1-Ccz1-RMC1 complex was determined using cryogenic-electron microscopy. RMC1 acts as a scaffolding subunit and binds to both Mon1 and Ccz1, explaining the binding specificity of the complex. The assembly of RMC1 with Mon1-Ccz1 is required for cellular RAB7A activation, autophagic functions, and organismal development in zebrafish.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biochemistry & Molecular Biology
Karina Schneider, Theresa Farr, Niko Pinter, Kerstin Schmitt, Oliver Valerius, Gerhard H. Braus, Joerg Kaemper
Summary: Early endosomes play important roles in long distance transport of cargoes in filamentous fungi, controlled by specific membrane-bound proteins such as Rab5 and tethering complexes like CORVET. In Ustilago maydis, Rab5a recruits CORVET to early endosomes, with Nma1 protein modulating the lifespan of early endosomes to ensure their availability for transportation processes.
MOLECULAR MICROBIOLOGY
(2022)
Article
Cell Biology
Yuan-Jing Zou, Meng-Meng Shan, Hong-Hui Wang, Zhen-Nan Pan, Meng-Hao Pan, Yi Xu, Jia-Qian Ju, Shao-Chen Sun
Summary: Our study investigated the roles of RAB14 during oocyte meiotic maturation, showing that depletion of RAB14 caused large polar bodies and defects in spindle migration. The regulation on actin-based spindle migration and Golgi apparatus distribution by RAB14 during mouse oocyte meiotic maturation was suggested by our results.
CELL PROLIFERATION
(2021)
Article
Plant Sciences
Robert G. Orr, Fabienne Furt, Erin L. Warner, Erin M. Agar, Jennifer M. Garbarino, Sarah E. Cabral, Michelle L. Dubuke, Allison M. Butt, Mary Munson, Luis Vidali
Summary: The interaction between Rab-E and myosin XI is a conserved feature of polarised growth in plants, and it is essential for the normal plant growth. This interaction is specifically mediated by a certain residue in myosin XI, and it supports the spatial and temporal coupling of both proteins at sites of active exocytosis.
Article
Biochemistry & Molecular Biology
Bingkuan Xu, Shuai Huang, Yinghui Liu, Chun Wan, Yuanyuan Gu, Dianliang Wang, Haijia Yu
Summary: The major protein component of Lewy bodies, alpha-Synuclein, is found to aggregate faster with the presence of Mn2+ ions, which regulate the protein phase separation process. Mn2+ not only promotes the transition of alpha-Synuclein from liquid to solid phase, but also induces soluble alpha-Synuclein monomers to form solid-like condensates. This phase transition facilitated the aggregation of alpha-Synuclein. Additionally, the integration of lipid membranes into the condensates during the Mn2+-induced alpha-Synuclein phase transition was observed.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Shuai Huang, Xiaoli Mo, Jieyi Wang, Xinyi Ye, Haijia Yu, Yinghui Liu
Summary: The aggregation of alpha-synuclein is a key pathological feature of Parkinson's disease. This study reveals that the C-terminal region of alpha-synuclein regulates its liquid-liquid phase separation through electrostatic interactions. Additionally, truncated forms of alpha-synuclein can promote phase separation and amyloid aggregation. These findings shed light on the role of alpha-synuclein in Parkinson's disease pathology.
Article
Cell Biology
Tiantian Qian, Chenlu Li, Furong Liu, Kai Xu, Chun Wan, Yinghui Liu, Haijia Yu
Summary: Endoplasmic reticulum-plasma membrane contact sites (EPCSs) in plant cells play a critical role in abiotic stress tolerance. Arabidopsis SYT1, enriched in EPCSs, has been shown to mediate lipid transfer between membranes. The lipid transfer activity of SYT1 is regulated by phosphatidylinositol 4,5-bisphosphate [PI(4,5)P-2] and Ca2+.
Article
Biochemistry & Molecular Biology
Bingkuan Xu, Xiaoli Mo, Jing Chen, Haijia Yu, Yinghui Liu
Summary: This study found that myricetin can inhibit amyloid aggregation in the condensates of α-synuclein by retarding the liquid-to-solid phase transition and disassembling preformed amyloid aggregates.
Article
Radiology, Nuclear Medicine & Medical Imaging
Jingchao Li, Luqian Cui, Lingkun Ma, Haijia Yu, Huihui Song, Shujuan Dong, Yingjie Chu
Summary: This study aimed to compare the clinical characteristics and predictive value of new-onset atypical right branch bundle block (ATRBBB) in short-term and long-term mortality with the typical right branch bundle block (TRBBB) subset in acute myocardial infarction (AMI) patients. The study found that the ATRBBB group had more severe clinical symptoms and prognosis. New ATRBBB was identified as an independent predictor of in-hospital major adverse cardiovascular events (MACE) and one-year mortality in patients with AMI combined with right branch bundle block (RBBB). Immediate opening of the infarct-related vessel may prevent the development of TRBBB to ATRBBB and improve prognosis.
CONTRAST MEDIA & MOLECULAR IMAGING
(2022)
Article
Biochemistry & Molecular Biology
Furong Liu, Ruyue He, Min Zhu, Lin Zhou, Yinghui Liu, Haijia Yu
Summary: In this study, the researchers found that Munc18c protein facilitates SNARE-dependent membrane fusion through an important peptide fragment called SLP. This discovery suggests that the t-SNARE-SLP binding mode might be a conserved mechanism for the stimulatory function of SM proteins in vesicle fusion.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Min Zhu, Han Xu, Yuting Jiang, Haijia Yu, Yinghui Liu
Summary: Insulin secretion is a signal-triggered process that involves membrane fusion mediated by SNAREs. This study reveals that EGCG efficiently inhibits SNARE-mediated insulin secretory vesicle fusion, making it a potential regulator of SNARE-dependent insulin secretion.
Article
Biology
Jinlong Yin, Xin Hong, Sha Luo, Jingquan Tan, Yuanming Zhang, Yanglin Qiu, Muhammad Faizan Latif, Tao Gao, Haijia Yu, Jingke Bai, Shujun Li, Kai Xu
Summary: This study investigates the epidemic of wild tomato mosaic virus (WTMV) in China and constructs an infectious DNA clone. The results show that WTMV can infect wild eggplant, black nightshade, and tobacco plants but not various local pepper varieties. WTMV is compatible with coinfection of cucumber mosaic virus (CMV) or tobacco mosaic virus (TMV) in tobacco, but not other potyviruses. This research provides important insights into WTMV evolution, infectivity, and recent epidemics, and offers a key tool for further studies of disease-resistance and field management.
Article
Cell Biology
Wenjing Sun, Hongquan Lu, Shihua Cui, Shenghui Zhao, Haijia Yu, Huihui Song, Qiuyue Ruan, Yabin Zhang, Yingjie Chu, Shujuan Dong
Summary: This study found that NEDD4 inhibits NLRP3 inflammasome activation and pyroptosis by directly interacting with SF3A2 and indirectly promoting proteasomal degradation of NLRP3. Inhibiting NLRP3 inflammasome activation also prevents cardiac microvascular endothelial cell injury. NEDD4 could be a potential therapeutic target for microvascular injury following myocardial reperfusion.
CELL COMMUNICATION AND SIGNALING
(2023)
Editorial Material
Multidisciplinary Sciences
Haijia Yu, Jingshi Shen
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Review
Biotechnology & Applied Microbiology
Fengzhi Cui, Jianhua Liu, Tianqi Zhang, Siwen Pang, Haijia Yu, Nannan Xu
Summary: The burden of cancer is increasing, being widely recognized as one of the main reasons for deaths among humans. Nanomedicine has become increasingly important in precision oncotherapy, improving the diagnosis, treatment, and prognosis of cancer. Low-dimensional nanomaterials (LDNs) with unique physicochemical capabilities have great potential for advanced biomedical applications, allowing targeted therapy and efficacy assessments for cancer. This review aims to summarize the classification and major properties of LDNs, focusing on their applications in cancer imaging, diagnosis, and treatment.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Cardiac & Cardiovascular Systems
Luqian Cui, Shihua Cui, Shujuan Dong, Jingchao Li, Haijia Yu, Huihui Song, Yongmei Han, Yingjie Chu
Summary: The AI values were predictive of the acute conduction block of SVCI. Differentiation between lateral and non-lateral sites was achieved based on specific AI cut-off values.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Ruyue He, Furong Liu, Hui Wang, Shuai Huang, Kai Xu, Conggang Zhang, Yinghui Liu, Haijia Yu
Summary: In this study, it was discovered that ORP9 and ORP10 form a binary complex through intermolecular coiled-coil domain interaction. The PH domains of ORP9 and ORP10 specifically interact with PI4P and play a critical role in TGN targeting and regulating PI4P levels. ORP9 and ORP10 complex accelerate the transfer of PI4P between membranes and their PH domains participate in membrane tethering, while the ORD domains are required for lipid transport. Depletion of ORP9 and ORP10 leads to increased vesicle transport to the plasma membrane.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Wen Luo, Zhibin Zhang, Dongtao Zhou, Yateng Jiang, Jingjing Yang, Bangshun He, Haijia Yu, Yujun Song
Summary: Improving penetration of therapeutic agents and activating immunity in tumor centers is crucial in cancer therapies. In this study, polydopamine-coated Escherichia coli Nissle 1917 (EcN) carrying liposomes loaded with CRISPR-Cas9 plasmid (Lipo-P) demonstrated enhanced immunotherapy in deep tumors by activating innate and adaptive immune responses simultaneously. The use of bacterial-assisted photothermal-sensitized immunotherapy shows promising results in deep tumors.
Article
Chemistry, Multidisciplinary
Wen Luo, Zhibin Zhang, Dongtao Zhou, Yateng Jiang, Jingjing Yang, Bangshun He, Haijia Yu, Yujun Song
Summary: In this study, polydopamine-coated Escherichia coli Nissle 1917 (EcN) bearing CRISPR-Cas9 plasmid-loaded liposomes (Lipo-P) were used for enhanced immunotherapy in deep tumors through activation of innate and adaptive immunity simultaneously. The Lipo-P accumulated in the tumor center through hypoxia targeting and could be detached under the reduction of reactive oxygen species (ROS)-responsive linkers, leading to thermal resistance reduction and effective tumor ablation. Additionally, the mild photothermal therapy induced immunogenic cell death and triggered innate immunity through bacterial infections in tumor tissues.