Article
Plant Sciences
Yanyan Cao, Qiannan Diao, Youyuan Chen, Haijun Jin, Yongping Zhang, Hongmei Zhang
Summary: A novel QTL named qCmPMR-12 for powdery mildew resistance in melon was identified on chromosome 12, ranging from 22.0 Mb to 22.9 Mb. The candidate gene MELO3C002434 encoding an ankyrin repeat-containing protein was suggested to be associated with resistance to PM. Furthermore, 15 polymorphic SNPs were successfully converted to KASP markers with high significance (P < 0.0001).
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Shuai Hou, Fangkun Wu, Zhiqiang Wang, Ning Yan, Hao Chen, Haojie Li, Peiyu Yang, Ying Zhang, Caixia Li, Yu Lin, Jian Ma, Lin Huang, Yaxi Liu
Summary: Stripe rust is a destructive disease that significantly reduces wheat yields globally. To control this disease, we developed a resistant cultivar called 'N2496', which showed high resistance and could be used in breeding programs. Through crossing 'N2496' with common wheat cultivars, we identified a quantitative trait locus (QTL) for stripe rust resistance on chromosome arm 6BL. The QTL explained a high percentage of phenotypic variation and was validated in other populations.
Article
Plant Sciences
Xi Yang, Jingyi Wang, Xinguo Mao, Chaonan Li, Long Li, Yinghong Xue, Liheng He, Ruilian Jing
Summary: This study analyzed leaf rolling degree in wheat and identified a major locus, named LERD1, on chromosome 7A that is associated with leaf rolling degree. Twenty-one candidate genes were predicted in this locus, and TraesCS7A01G543300, a basic helix-loop-helix transcription factor, was identified as the most likely candidate gene for LERD1.
Article
Plant Sciences
Huyen Bui, Robert Greenhalgh, Gunbharpur S. Gill, Meiyuan Ji, Andre H. Kurlovs, Christian Ronnow, Sarah Lee, Ricardo A. Ramirez, Richard M. Clark
Summary: This study identified certain maize lines with high resistance to Tetranychus urticae, but not to Oligonychus pratensis. It found QTL on chromosome 6 related to resistance to T. urticae, part of which originated from line B96.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Huangkai Zhou, Kuanqiang Tang, Guang Li, Wenqiang Liu, Hui Yu, Xiaohui Yuan, Suxin Yang, Madan K. Bhattacharyya, Xianzhong Feng
Summary: The M2-seq method enables efficient and rapid mapping of candidate mutant genes in the M-2 generation, removing background variations and aiding in the identification of causal mutations.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Junmei Wang, Yahong Li, Fei Xu, Hongxing Xu, Zihang Han, Lulu Liu, Yuli Song
Summary: Utilizing SLAF technology and BSR-seq method, a candidate region for pmHYM on chromosome 7BL of wheat landrace cultivar Hongyoumai was identified, with differential gene expression and a potential candidate gene revealed through gene cloning.
Article
Biochemistry & Molecular Biology
Xiangping Dai, Takashi Kiuchi, Yanyan Zhou, Shunze Jia, Yusong Xu, Susumu Katsuma, Toru Shimada, Huabing Wang
Summary: The study conducted a comprehensive analysis of the evolution of insect beta-fructofuranosidase transferred through HGT, identifying posttransfer duplications in Lepidoptera and mutations affecting physiological function in Bombyx mori. These findings indicate that gene transfer enhances the digestive and metabolic adaptation of lepidopteran insects.
MOLECULAR BIOLOGY AND EVOLUTION
(2021)
Article
Genetics & Heredity
Ting Fang, Yiwei Bai, Wenxuan Huang, Yueying Wu, Zhihui Yuan, Xiaoyan Luan, Xinlei Liu, Lianjun Sun
Summary: This study investigated the gene regulatory network affecting the ratio of four-seed pods in soybean using bulked segregant RNA sequencing technology. Potential gene regulation pathways associated with the ratio of four-seed pods were identified, providing insights into the genetic basis of this agronomic trait.
FRONTIERS IN GENETICS
(2021)
Article
Plant Sciences
K. Stephen, K. Aparna, R. Beena, R. P. Sah, Uday Chand Jha, Sasmita Behera
Summary: The damage caused by high temperature is a significant abiotic stress on rice production. In this study, a heat tolerant rice cultivar (NERICA-L 44) and a heat susceptible cultivar (Uma) were crossed to generate F1 and F2 populations. The F2 population was exposed to heat stress and evaluated for tolerance. The analysis revealed markers associated with heat tolerance and identified a newly reported marker (RM337) linked to high temperature tolerance in rice.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
Yue Zhao, Yufeng Sun, Kun Cao, Xiaoyan Zhang, Jing Bian, Chengwei Han, Ying Jiang, Lei Xu, Xiaonan Wang
Summary: In this study, the SLAF-Seq and Bulked Segregant analysis (BSA) techniques were used to rapidly identify genes related to hemp fiber content. By extracting and sequencing DNA from hybrid plants, 25,133 high-quality SNPs were detected and 15 genes highly likely to regulate fiber content were identified. Further validation confirmed a strong positive correlation between six of these genes and hemp fiber content. This research provides novel mechanistic inputs for the fast identification of genes related to important agronomic traits of hemp and other crops in the textile industry.
Article
Biochemistry & Molecular Biology
Pei Li, Guo Li, Ya-Wen Zhang, Jian-Fang Zuo, Jin-Yang Liu, Yuan-Ming Zhang
Summary: This study proposes an integrated strategy combining BSA and whole-genome sequencing for mapping various types of QTLs. The method predicts genotypes and constructs a new statistic, G(w), for detecting quantitative trait genes. In simulated and real dataset analyses, this method outperforms traditional BSA and QTL mapping methods.
PLANT COMMUNICATIONS
(2022)
Article
Plant Sciences
Nuria F. Bermejo, Ghita Hoummadi, Sergi Munne-Bosch
Summary: Chia seeds are highly valued for their nutritional benefits, but chia sprouts have even better nutritional properties, particularly in terms of beta-carotene content. This study found that methyl salicylate can significantly increase the beta-carotene content in chia sprouts without affecting their fresh weight or germination rate. While further research is needed before industrial application, it is evident that methyl salicylate shows promise as a method to enhance beta-carotene levels in chia sprouts.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Article
Biotechnology & Applied Microbiology
Joshua G. Philips, Elena Martin-Avila, Andrea V. Robold
Summary: This review provides updated evidence on the likelihood, factors, and barriers for horizontal gene transfer in genetically modified plants, and presents the legislation and frameworks followed by the Australian Gene Technology Regulator in considering the risks posed by horizontal gene transfer.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Xu Ding, Jie-Xia Liu, Tong Li, Ao-Qi Duan, Lian Yin, Hao Wang, Li-Li Jia, Yan-Hua Liu, Hui Liu, Jian-Ping Tao, Ai-Sheng Xiong
Summary: The overexpression of AgZDS gene in Arabidopsis thaliana and celery resulted in increased accumulation of lutein and beta-carotene and up-regulated expression levels of genes involved in carotenoid biosynthesis. Additionally, the antioxidant capacity of A. thaliana and photosynthetic capacity of celery were enhanced in transgenic plants.
Article
Agriculture, Multidisciplinary
Yan Zhang, Mingzhe Li, Baoping Zhou, Zaibin Wang
Summary: This paper proposes an automatic cotton spider mites' damage grading algorithm for improved deep residual network based on transfer learning (C-ResNet50) to address the problems of low accuracy, time consumption, and high cost in traditional artificial diagnosis of cotton spider mites. By collecting images of cotton spider mites and healthy cotton leaf under different backgrounds, a dataset was constructed and C-ResNet50 was improved and compared with other models. The experimental results showed that C-ResNet50 achieved the best classification effect with an average accuracy rate of 98.10% in identifying the level of cotton spider mites damage.
COMPUTERS AND ELECTRONICS IN AGRICULTURE
(2023)
Article
Agronomy
Catherine Mermans, Wannes Dermauw, Sven Geibel, Thomas Van Leeuwen
Summary: Afoxolaner demonstrated strong antagonistic activity against GABACls of T. urticae, making it a powerful tool for controlling spider mites. In contrast, other drugs such as fluralaner, fipronil, and endosulfan showed minimal antagonistic activities against GABACls channels of T. urticae.
PEST MANAGEMENT SCIENCE
(2023)
Article
Ecology
Dylan M. Klure, Robert Greenhalgh, Thomas L. Parchman, Marjorie D. Matocq, Lanie M. Galland, Michael D. Shapiro, M. Denise Dearing
Summary: In a shrubland in California, a 3-year recapture study on Bryant's woodrat and desert woodrat revealed a high frequency of hybridization. The hybrids have comparable survival rates to the dominant parental species, indicating that ecological factors have limited influence on hybridization in this environment.
Article
Entomology
Masahiro Osakabe, Satoshi Shimano
Summary: A study found that the flashy red pigment of the red velvet mite consists of astaxanthin and 3-hydroxyechinenone, with a small amount of beta-carotene. The concentration of astaxanthin is 127 times higher in the red velvet mite compared to other mites. These keto-carotenoids with high antioxidant activity may contribute to the survival of the red velvet mite in harsh environments with intense sunlight, UV-B radiation, and radiant heat.
EXPERIMENTAL AND APPLIED ACAROLOGY
(2023)
Article
Agronomy
Konstantinos Mavridis, Aris Ilias, Kyriaki Maria Papapostolou, Kyriaki Varikou, Kleita Michaelidou, Anastasia Tsagkarakou, John Vontas
Summary: Molecular diagnostics have been developed for monitoring insecticide resistance in western flower thrips populations, providing evidence-based strategies for resistance management.
PEST MANAGEMENT SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Nedal Darif, Kathrin Vogelsang, Elena Vorgia, David Schneider, Elena Deligianni, Sven Geibel, John Vontas, Shane Denecke
Summary: Cell penetrating peptides (CPPs) are small peptides that can deliver molecular cargo into cells. This study characterized the entry of four fluorescently tagged CPPs into insect cells and dissected midgut tissues. The results showed that CPPs can penetrate the plasma membrane of ovarian and midgut-derived lepidopteran cells through endosomal uptake and that this process can be inhibited by specific endocytosis inhibitors. The study also found differences in the quantity and mode of penetration among different CPPs, with CPP-1838 showing the highest efficiency in penetrating membranes through passive diffusion.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2023)
Article
Virology
Sofia Balaska, Mattia Calzolari, Annalisa Grisendi, Mara Scremin, Michele Dottori, Konstantinos Mavridis, Romeo Bellini, John Vontas
Summary: The expanding distribution of sand flies, especially in Italy, is leading to an increase in the incidence of visceral leishmaniasis. Regular monitoring is needed to ensure the sustainability and effectiveness of vector control interventions, despite the widespread use of insecticides.
Article
Biochemistry & Molecular Biology
Adam Pym, John G. M. Mina, Bartlomiej J. Troczka, Angela Hayward, Eve Daum, Jan Elias, Russell Slater, John Vontas, Chris Bass, Christoph T. Zimmer
Summary: The tobacco whitefly, Bemisia tabaci, is a polyphagous crop pest that causes significant economic damage worldwide. Neonicotinoids are commonly used insecticides for the control of this species. Understanding the mechanisms responsible for resistance to neonicotinoids in B. tabaci is crucial for effective control and damage limitation. This study reveals that qualitative changes in the cytochrome P450 gene CYP6CM1 alter its metabolic capacity and enhance detoxification of neonicotinoids.
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Xueping Lu, Marilou Vandenhole, Dimitra Tsakireli, Spiros A. Pergantis, John Vontas, Wim Jonckheere, Thomas Van Leeuwen
Summary: Acequinocyl and bifenazate, as powerful acaricides, have been found to induce resistance development in spider mites. Target-site resistance resulting from mutations in the Qo pocket of cytochrome b has been identified as the main resistance mechanism. A new mutation, L258F, has been discovered in field populations of Tetranychus urticae, and its role in resistance has been confirmed. However, the resistance in this strain is likely due to a combination of target-site resistance and increased detoxification through P450 enzymes.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2023)
Article
Infectious Diseases
Frederic Labbe, Maha Abdeladhim, Jenica Abrudan, Alejandra Saori Araki, Ricardo N. Araujo, Peter Arensburger, Joshua B. Benoit, Reginaldo Pecanha Brazil, Rafaela V. Bruno, Gustavo Bueno da Silva Rivas, Vinicius Carvalho de Abreu, Jason Charamis, Iliano V. Coutinho-Abreu, Samara G. da Costa-Latge, Alistair Darby, Viv M. Dillon, Scott J. Emrich, Daniela Fernandez-Medina, Nelder Figueiredo Gontijo, Catherine M. Flanley, Derek Gatherer, Fernando A. Genta, Sandra Gesing, Gloria I. Giraldo-Calderon, Bruno Gomes, Eric Roberto Guimaraes Rocha Aguiar, James G. C. Hamilton, Omar Hamarsheh, Mallory Hawksworth, Jacob M. Hendershot, Paul V. Hickner, Jean-Luc Imler, Panagiotis Ioannidis, Emily C. Jennings, Shaden Kamhawi, Charikleia Karageorgiou, Ryan C. Kennedy, Andreas Krueger, Jose M. Latorre-Estivalis, Petros Ligoxygakis, Antonio Carlos A. Meireles-Filho, Patrick Minx, Jose Carlos Miranda, Michael J. Montague, Ronald J. Nowling, Fabiano Oliveira, Joao Ortigao-Farias, Marcio G. Pavan, Marcos Horacio Pereira, Andre Nobrega Pitaluga, Roenick Proveti Olmo, Marcelo Ramalho-Ortigao, Jose M. C. Ribeiro, Andrew J. Rosendale, Mauricio R. V. Sant'Anna, Steven E. Scherer, Nagila F. C. Secundino, Douglas A. Shoue, Caroline da Silva Moraes, Joao Silveira Moledo Gesto, Nataly Araujo Souza, Zainulabueddin Syed, Samuel Tadros, Rayane Teles-de-Freitas, Erich L. Telleria, Chad Tomlinson, Yara M. Traub-Cseko, Joao Trindade Marques, Zhijian Tu, Maria F. Unger, Jesus Valenzuela, Flavia Ferreira, Karla P. V. de Oliveira, Felipe M. Vigoder, John Vontas, Lihui Wang, Gareth D. Weedall, Elyes Zhioua, Stephen Richards, Wesley C. Warren, Robert M. Waterhouse, Rod J. Dillon, Mary Ann McDowell
Summary: Phlebotomine sand flies are important vectors of human disease, transmitting Leishmania parasites that cause devastating diseases. This study sequenced the genomes of two important sand fly vector species and identified genes involved in disease transmission and population structures. The findings provide a foundation for future efforts to prevent the spread of Leishmania parasites.
PLOS NEGLECTED TROPICAL DISEASES
(2023)
Article
Food Science & Technology
Rafaela Panteleri, Amalia Anthousi, Shane Denecke, Debora Boaventura, Ralf Nauen, John Vontas
Summary: The fall armyworm is a global invasive agricultural pest causing major crop losses. Control strategies rely on insecticides and transgenic crops, but resistance development is a significant issue. Mutations in the SfABCC2 gene have been associated with Bt toxin resistance in the fall armyworm. This study expressed SfABCC2 in Drosophila melanogaster to validate resistance mutations and cross-resistance issues.
Article
Agronomy
Emre Inak, Berke Demirci, Marilou Vandenhole, Gokhan Soylemezoglu, Thomas Van Leeuwen, Umut Toprak
Summary: Understanding the molecular mechanisms of resistance and cross-resistance between different ketoenol acaricides is crucial to manage resistance. P450 monooxygenases and carboxyl/cholinesterases were found to be involved in resistance. Gene expression analysis confirmed the involvement of P450-mediated resistance, while no target-site mutations were detected.
Article
Entomology
Yumiko Oide, Masahiro Osakabe
Summary: An incomplete species recognition system can result in reproductive interference, leading to reproductive problems in F-1 hybrid females produced from interspecific mating between Panonychus citri and Panonychus osmanthi. The competitiveness of P. citri males was higher, but their species recognition ability was lower compared to P. osmanthi males, indicating the occurrence of potential reproductive interference. The study scored 7 out of 10.
EXPERIMENTAL AND APPLIED ACAROLOGY
(2023)
Article
Parasitology
France-Paraudie A. Kouadio, Nadja C. Wipf, Angele S. Nygble, Behi K. Fodjo, Christabelle G. Sadia, John Vontas, Konstantinos Mavridis, Pie Mueller, Chouaibou S. Mouhamadou
Summary: This study aims to investigate the impact of different agricultural practices on phenotypic insecticide resistance and associated molecular resistance mechanisms in Anopheles gambiae s.l. The results show that mosquito populations from all agricultural sites were resistant to several insecticides, with variation in the frequency of resistance mutations and gene expression levels. The findings suggest that agricultural practices might have a role in shaping insecticide resistance profiles in malaria vectors.
PARASITES & VECTORS
(2023)
Article
Agronomy
Christine Njiru, Corinna Saalwaechter, Konstantinos Mavridis, John Vontas, Sven Geibel, Nicky Wybouw, Thomas Van Leeuwen
Summary: A target site mutation in a spider mite pest has been found to confer resistance to certain acaricides and has significant fitness costs. These findings highlight the importance of considering multiple factors in assessing the impact of resistance mutations on pest populations.
PEST MANAGEMENT SCIENCE
(2023)
Article
Agronomy
Masaya Tanaka, Junya Yase, Takeshi Kanto, Masahiro Osakabe
Summary: This study investigates the control of pests through non-chemical methods and finds that green pest control methods are more sustainable, cost-effective, and feasible compared to traditional pesticide-based approaches.
PEST MANAGEMENT SCIENCE
(2023)