Cas1 and the Csy complex are opposing regulators of Cas2/3 nuclease activity

A CRISPR evolutionary arms race: structural insights into viral anti-CRISPR/Cas responses

(2016) Jiuyu Wang et al.   CELL RESEARCH

Deciphering and shaping bacterial diversity through CRISPR

(2016) Alexandra E Briner et al.   CURRENT OPINION IN MICROBIOLOGY

Requirements for Pseudomonas aeruginosa Type I-F CRISPR-Cas Adaptation Determined Using a Biofilm Enrichment Assay

(2016) Gary E. Heussler et al.   JOURNAL OF BACTERIOLOGY

Adaptation in CRISPR-Cas Systems

(2016) Samuel H. Sternberg et al.   MOLECULAR CELL

Cas3-Derived Target DNA Degradation Fragments Fuel Primed CRISPR Adaptation

(2016) Tim Künne et al.   MOLECULAR CELL

Structural basis for promiscuous PAM recognition in type I–E Cascade from E. coli

(2016) Robert P. Hayes et al.   NATURE

CRISPR–Cas adaptation: insights into the mechanism of action

(2016) Gil Amitai et al.   NATURE REVIEWS MICROBIOLOGY

A molecular arms race: new insights into anti-CRISPR mechanisms

(2016) John Mallon et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Protecting genome integrity during CRISPR immune adaptation

(2016) Addison V Wright et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Structural basis of Cas3 inhibition by the bacteriophage protein AcrF3

(2016) Xiaofei Wang et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Diverse evolutionary roots and mechanistic variations of the CRISPR-Cas systems

(2016) Prarthana Mohanraju et al.   SCIENCE

Interference-driven spacer acquisition is dominant over naive and primed adaptation in a native CRISPR–Cas system

(2016) Raymond H. J. Staals et al.   Nature Communications

A molecular arms race: new insights into anti-CRISPR mechanisms

(2016) John Mallon et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Protecting genome integrity during CRISPR immune adaptation

(2016) Addison V Wright et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Structural basis of Cas3 inhibition by the bacteriophage protein AcrF3

(2016) Xiaofei Wang et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Inactivation of CRISPR-Cas systems by anti-CRISPR proteins in diverse bacterial species

(2016) April Pawluk et al.   Nature Microbiology

Surveillance and Processing of Foreign DNA by the Escherichia coli CRISPR-Cas System

(2015) Sy Redding et al.   CELL

SnapShot: CRISPR-RNA-Guided Adaptive Immune Systems

(2015) Joshua Carter et al.   CELL

Structural and Mechanistic Basis of PAM-Dependent Spacer Acquisition in CRISPR-Cas Systems

(2015) Jiuyu Wang et al.   CELL

CTFFIND4: Fast and accurate defocus estimation from electron micrographs

(2015) Alexis Rohou et al.   JOURNAL OF STRUCTURAL BIOLOGY

A Conserved Structural Chassis for Mounting Versatile CRISPR RNA-Guided Immune Responses

(2015) Ryan N. Jackson et al.   MOLECULAR CELL

Two Distinct DNA Binding Modes Guide Dual Roles of a CRISPR-Cas Protein Complex

(2015) Timothy R. Blosser et al.   MOLECULAR CELL

Discovery and Functional Characterization of Diverse Class 2 CRISPR-Cas Systems

(2015) Sergey Shmakov et al.   MOLECULAR CELL

Integrase-mediated spacer acquisition during CRISPR–Cas adaptive immunity

(2015) James K. Nuñez et al.   NATURE

Foreign DNA capture during CRISPR–Cas adaptive immunity

(2015) James K. Nuñez et al.   NATURE

CRISPR-Cas immunity in prokaryotes

(2015) Luciano A. Marraffini   NATURE

Multiple mechanisms for CRISPR–Cas inhibition by anti-CRISPR proteins

(2015) Joseph Bondy-Denomy et al.   NATURE

An updated evolutionary classification of CRISPR–Cas systems

(2015) Kira S. Makarova et al.   NATURE REVIEWS MICROBIOLOGY

Mechanism of foreign DNA recognition by a CRISPR RNA-guided surveillance complex from Pseudomonas aeruginosa

(2015) MaryClare F. Rollins et al.   NUCLEIC ACIDS RESEARCH

Foreign DNA acquisition by the I-F CRISPR–Cas system requires all components of the interference machinery

(2015) Daria Vorontsova et al.   NUCLEIC ACIDS RESEARCH

Different genome stability proteins underpin primed and naïve adaptation inE. coliCRISPR-Cas immunity

(2015) Ivana Ivančić-Baće et al.   NUCLEIC ACIDS RESEARCH

Directional R-Loop Formation by the CRISPR-Cas Surveillance Complex Cascade Provides Efficient Off-Target Site Rejection

(2015) Marius Rutkauskas et al.   Cell Reports

Recurrent turnover of senescent cells during regeneration of a complex structure

(2015) Maximina H Yun et al.   eLife

Fitting CRISPR-associated Cas3 into the Helicase Family Tree

(2014) Ryan N. Jackson et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

DNA interrogation by the CRISPR RNA-guided endonuclease Cas9

(2014) Samuel H. Sternberg et al.   NATURE

Structures of CRISPR Cas3 offer mechanistic insights into Cascade-activated DNA unwinding and degradation

(2014) Yanwu Huo et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Cas1–Cas2 complex formation mediates spacer acquisition during CRISPR–Cas adaptive immunity

(2014) James K Nuñez et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Detection and characterization of spacer integration intermediates in type I-E CRISPR–Cas system

(2014) Zihni Arslan et al.   NUCLEIC ACIDS RESEARCH

Priming in the Type I-F CRISPR-Cas system triggers strand-independent spacer acquisition, bi-directionally from the primed protospacer

(2014) Corinna Richter et al.   NUCLEIC ACIDS RESEARCH

Molecular insights into DNA interference by CRISPR-associated nuclease-helicase Cas3

(2014) Bei Gong et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Direct observation of R-loop formation by single RNA-guided Cas9 and Cascade effector complexes

(2014) Mark D. Szczelkun et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

CasA mediates Cas3-catalyzed target degradation during CRISPR RNA-guided interference

(2014) M. L. Hochstrasser et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Degenerate target sites mediate rapid primed CRISPR adaptation

(2014) P. C. Fineran et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

In vitro reconstitution of Cascade-mediated CRISPR immunity in Streptococcus thermophilus

(2013) Tomas Sinkunas et al.   EMBO JOURNAL

In VitroReconstitution of anEscherichia coliRNA-guided Immune System Reveals Unidirectional, ATP-dependent Degradation of DNA Target

(2013) Sabin Mulepati et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Comparative genomics of defense systems in archaea and bacteria

(2013) Kira S. Makarova et al.   NUCLEIC ACIDS RESEARCH

In defense of phage

(2013) Blake Wiedenheft   RNA Biology

The CRISPR/Cas Adaptive Immune System of Pseudomonas aeruginosa Mediates Resistance to Naturally Occurring and Engineered Phages

(2012) K. C. Cady et al.   JOURNAL OF BACTERIOLOGY

RELION: Implementation of a Bayesian approach to cryo-EM structure determination

(2012) Sjors H.W. Scheres   JOURNAL OF STRUCTURAL BIOLOGY

CRISPR Immunity Relies on the Consecutive Binding and Degradation of Negatively Supercoiled Invader DNA by Cascade and Cas3

(2012) Edze R. Westra et al.   MOLECULAR CELL

Bacteriophage genes that inactivate the CRISPR/Cas bacterial immune system

(2012) Joe Bondy-Denomy et al.   NATURE

Proteins and DNA elements essential for the CRISPR adaptation process in Escherichia coli

(2012) Ido Yosef et al.   NUCLEIC ACIDS RESEARCH

CRISPR Interference Directs Strand Specific Spacer Acquisition

(2012) Daan C. Swarts et al.   PLoS One

In Vivo Protein Interactions and Complex Formation in the Pectobacterium atrosepticum Subtype I-F CRISPR/Cas System

(2012) Corinna Richter et al.   PLoS One

Cascade-mediated binding and bending of negatively supercoiled DNA

(2012) Edze R. Westra et al.   RNA Biology

Molecular memory of prior infections activates the CRISPR/Cas adaptive bacterial immunity system

(2012) Kirill A. Datsenko et al.   Nature Communications

Structure and activity of the Cas3 HD nuclease MJ0384, an effector enzyme of the CRISPR interference

(2011) Natalia Beloglazova et al.   EMBO JOURNAL

Cas3 is a single-stranded DNA nuclease and ATP-dependent helicase in the CRISPR/Cas immune system

(2011) Tomas Sinkunas et al.   EMBO JOURNAL

Structural and Biochemical Analysis of Nuclease Domain of Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)-associated Protein 3 (Cas3)

(2011) Sabin Mulepati et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

RNA-guided complex from a bacterial immune system enhances target recognition through seed sequence interactions

(2011) B. Wiedenheft et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Interference by clustered regularly interspaced short palindromic repeat (CRISPR) RNA is governed by a seed sequence

(2011) E. Semenova et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Appion: An integrated, database-driven pipeline to facilitate EM image processing

(2009) Gabriel C. Lander et al.   JOURNAL OF STRUCTURAL BIOLOGY

DoG Picker and TiltPicker: Software tools to facilitate particle selection in single particle electron microscopy

(2009) N.R. Voss et al.   JOURNAL OF STRUCTURAL BIOLOGY

Structural Basis for DNase Activity of a Conserved Protein Implicated in CRISPR-Mediated Genome Defense

(2009) Blake Wiedenheft et al.   STRUCTURE