期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 114, 期 17, 页码 E3424-E3433出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1701978114
关键词
SHPRH; rRNA transcription; histone H3 methylation; mTOR
资金
- National Human Genome Research Institute [HG200376-01]
- National Institute of Child Health and Human Developmen
- Institute for Basic Science [IBS-R022-D1-2015]
- National Research Foundation of Korea Ministry of Science, ICT, and Future Planning (MSIP) [2010-0028684]
Many DNA repair proteins have additional functions other than their roles in DNA repair. In addition to catalyzing PCNA polyubiquitylation in response to the stalling of DNA replication, SHPRH has the additional function of facilitating rRNA transcription by localizing to the ribosomal DNA (rDNA) promoter in the nucleoli. SHPRH was recruited to the rDNA promoter using its plant homeodomain (PHD), which interacts with histone H3 when the fourth lysine of H3 is not trimethylated. SHPRH enrichment at the rDNA promoter was inhibited by cell starvation, by treatment with actinomycin D or rapamycin, or by depletion of CHD4. SHPRH also physically interacted with the RNA polymerase I complex. Taken together, we provide evidence that SHPRH functions in rRNA transcription through its interaction with histone H3 in a mammalian target of rapamycin (mTOR)-dependent manner.
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