Article
Engineering, Biomedical
Lukasz Szymanski, Kamila Golaszewska, Anna Wiatrowska, Monika Dropik, Pawel Szymanski, Bartosz Gromadka, Patrycja Krakowiak, Justyna Wierzchowska, Damian Matak
Summary: This study evaluates the biocompatibility of 4SEAL® Hemostatic powder according to ISO 10993 standards. The results demonstrate that the powder meets the biocompatibility requirements and shows no cytotoxicity, mutagenic potential, toxicity, sensitization, irritation, or pyrogenicity. Chemical characterization analysis also reveals no compounds above the analytical evaluation threshold and no elements with concentrations higher than element-specific permissible daily exposure.
BIOMATERIALS RESEARCH
(2022)
Article
Engineering, Biomedical
Mehrnoosh Neshatian, James Holcroft, Anil Kishen, Grace De Souza, Bernhard Ganss
Summary: In this study, bio-inspired bio-nano complexes were created using amelotin (AMTN) or AMTN with an engineered collagen-binding site (AMTN-Col) to coat hydroxyapatite nanoparticles (HANP), and their ability to promote collagen mineralization was evaluated. The results showed that these bio-nano complexes could accelerate mineral formation and enhance the bond strength at the resin-dentin interface.
MATERIALS TODAY BIO
(2022)
Article
Biochemistry & Molecular Biology
Carolin Lindner, Said Alkildani, Sanja Stojanovic, Stevo Najman, Ole Jung, Mike Barbeck
Summary: The present study aimed to investigate the tissue responses and barrier functionality of a novel Guided Bone Regeneration (GBR) membrane composed of bovine collagen type I. The results showed that the bovine membrane exhibited similar host tissue reaction to porcine-based membranes and was fully biocompatible. The membrane showed good integration up to day 60 without material fragmentation, and allowed for secondary porosity and transmembrane vascularization at day 90. This suggests that the novel bovine barrier membrane is a suitable alternative to porcine-sourced membranes for GBR procedures.
Article
Biochemistry & Molecular Biology
James Bielenstein, Milena Radenkovic, Stevo Najman, Luo Liu, Yanru Ren, Baoyi Cai, Florian Beuer, Denis Rimashevskiy, Reinhard Schnettler, Said Alkildani, Ole Jung, Franziska Schmidt, Mike Barbeck
Summary: This study analyzes the in vivo responses to xenogeneic and synthetic bone substitute granules, revealing differences in bone regeneration and immune response. The synthetic BSM induces a higher pro-inflammatory response, while the xenogeneic BSM induces a higher anti-inflammatory reaction. However, both materials show comparable bone regeneration. The results demonstrate the biocompatibility of the synthetic BSM maxresorb (R) and suggest it as a suitable alternative to natural BSM for various indications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Bradley T. Estes, Masataka Enomoto, Franklin T. Moutos, Megan A. Carson, Jeffrey M. Toth, Peter Eggert, Jonathan Stallrich, Vincent P. Willard, Deborah J. Veis, Dianne Little, Farshid Guilak, B. Duncan X. Lascelles
Summary: The study demonstrated that a tissue-engineered bicomponent implant, combining new technologies and components, was able to restore hip function in a canine model of hip osteoarthritis. The implant showed potential as a therapy for moderate hip osteoarthritis, providing clinically relevant evidence of its efficacy.
Article
Biochemical Research Methods
Nikita Kovalenko, Kirill Sovin, Tatiana Malikova, Artur Smirnov, Vladimir Anpilov, Oleg Ryabushkin
Summary: A novel method for monitoring the state of biological tissues during optical treatment by measuring radiofrequency impedance kinetics has been introduced. Experimental applications demonstrate the method's high sensitivity and repeatability, making it a potential tool for selecting and optimizing radiation parameters of medical laser sources.
JOURNAL OF BIOPHOTONICS
(2022)
Article
Biochemistry & Molecular Biology
Fernando Bento Cunha, Karina Torres Pomini, Ana Maria de Guzzi Plepis, Virginia da Conceicao Amaro Martins, Eduardo Gomes Machado, Renato de Moraes, Marcelo de Azevedo e Souza Munhoz, Michela Vanessa Ribeiro Machado, Marco Antonio Hungaro Duarte, Murilo Priori Alcalde, Daniela Vieira Buchaim, Rogerio Leone Buchaim, Victor Augusto Ramos Fernandes, Eliana de Souza Bastos Mazuqueli Pereira, Andre Antonio Pelegrine, Marcelo Rodrigues da Cunha
Summary: The study investigated the effects of elastin and collagen matrix on bone repair in critical size defects in rat calvaria. The results showed that using these matrices as scaffolds was feasible and increased the formation of new bone tissue in critical size defects.
Article
Multidisciplinary Sciences
Katsutoshi Miura
Summary: The stiffness of lung tissue changes with aging. Scanning acoustic microscope (SAM), by calculating the speed-of-sound (SOS), can evaluate tissue stiffness and structure simultaneously. Aging lungs exhibit decreased SOS values, associated with the loss of smooth muscles, collagen, and elastic fibers.
Article
Chemistry, Physical
Yoshimitsu Okazaki, Shin-ichi Katsuda
Summary: The study demonstrated the excellent biocompatibility of Ti-Zr alloys in implantation, promoting bone formation. Different alloy compositions showed slight variations in their effects on surrounding tissues, and grit blasting could enhance the maximum pullout loads of the alloys.
Article
Multidisciplinary Sciences
Phong K. Nguyen, Christoph Hart, Kaitlyn Hall, Iverson Holt, Catherine K. Kuo
Summary: Adult tendons heal with scar tissue and have high re-injury rates, while fetal tendons can heal without scarring. However, there is limited understanding of fetal tendon healing due to a lack of accessible animal models. In this study, researchers developed an in vivo and ex vivo chick embryo tendon model to study fetal tendon healing. The results showed that tendon healing occurred faster in vivo, and tendons injured at an earlier embryonic stage showed improved mechanical properties. The expression levels of various markers, regulators, and mediators were found to be stage-dependent. Further studies using these models will help inform the development of regenerative therapies for adult tendons.
SCIENTIFIC REPORTS
(2023)
Article
Engineering, Biomedical
Jing Wang, He Qiu, Yang Xu, Yongli Gao, Peijie Tan, Rui Zhao, Zhanhong Liu, Yajun Tang, Xiangdong Zhu, Chongyun Bao, Hang Wang, Hai Lin, Xingdong Zhang
Summary: In this study, a skin damage animal model was prepared to investigate the biological effect of recombinant humanized type III collagen (rhCol III) on skin repair. The results indicated that rhCol III could alleviate skin photoaging and have an influence on cells, extracellular matrix, and small biological molecules.
BIOACTIVE MATERIALS
(2022)
Article
Food Science & Technology
Yingbo Peng, Wanhong He, Shuang Teng, Muneer Ahmed Jamali, Severiano R. Silva, Alfredo Jorge Costa Teixeira
Summary: In this study, we investigated the degradation of intramuscular connective tissue (IMCT) using cathepsin L. Our findings showed that cathepsin L mainly degrades the non-helical peptides of collagen in IMCT, rather than the triple helix region. We also observed that cathepsin L has a more pronounced degradation effect on the structural protein DCN at a pH of 5.0. Additionally, cathepsin L treatment resulted in a more significant decrease in the thermal denaturation temperatures (T-P and T-O) of IMCT, especially at a pH of 5.0.
Article
Chemistry, Multidisciplinary
Dominic Rutsche, Monica Nanni, Simon Rudisser, Thomas Biedermann, Marcy Zenobi-Wong
Summary: This study introduces a new collagen derivative with multiple recognition peptides for enzyme-mediated crosslinking, enabling the co-engineering of human blood and lymphatic microcapillaries. The study further demonstrates the formation of luminal structures and the maturation markers of bioengineered capillaries both in vitro and in vivo. The versatile collagen derivative is also utilized for spatially defined polymer compositions with pro- and anti-angiogenic properties, as well as the formation of vascularization independent from fibroblasts.
ADVANCED MATERIALS
(2023)
Article
Chemistry, Physical
Rone Aparecido De Grandis, Larissa Natiele Miotto, Luis Eduardo Genaro, Larissa Migliatti Polli, Ana Maria de Guzzi Plepis, Fabiana Tessari Rodrigues, Virginia da Conceicao Amaro Martins, Leonardo Pereira Franchi, Raquel Mantuaneli Scarel-Caminaga, Ticiana Sidorenko de Oliveira Capote
Summary: The aim of this study was to characterize PP matrices derived from porcine pericardium before and after sterilization, comparing ethylene oxide and gamma ray methods. After alkaline hydrolysis treatment, no significant changes in matrix thickness were observed, and the biodegradability of the material post-implantation was stable. Cell viability and genotoxicity assays showed that PP matrices did not have a significant impact on CHO-K1 cells, regardless of the sterilization method used.
Article
Engineering, Biomedical
Iulian Antoniac, Aurora Antoniac, Eugeniu Vasile, Camelia Tecu, Marco Fosca, Viktoriya G. Yankova, Julietta Rau
Summary: Novel composite scaffolds composed of collagen-hydroxyapatite and collagen-hydroxyapatite-magnesium were developed, showing promising potential for bone tissue engineering applications. The nanostructural properties and degradation behavior of the scaffolds were thoroughly investigated through advanced microscopy techniques.
BIOACTIVE MATERIALS
(2021)
