Article
Chemistry, Analytical
Eisuke Kanao, Shuntaro Wada, Hiroshi Nishida, Takuya Kubo, Tetsuya Tanigawa, Koshi Imami, Asako Shimoda, Kaori Umezaki, Yoshihiro Sasaki, Kazunari Akiyoshi, Jun Adachi, Koji Otsuka, Yasushi Ishihama
Summary: Extracellular vesicles (EVs) are lipid bilayer vesicles enclosing various biomolecules, showing potential as sensitive biomarkers for disease detection. A novel lectin-based affinity chromatography method is demonstrated to classify EVs based on their glycan structures, utilizing a spongy-like monolithic polymer that allows efficient in situ protein reaction. This approach helps to overcome the obstacle of differing molecular compositions of EVs from same donor cells, showing promise for elucidating their biological functions objectively.
ANALYTICAL CHEMISTRY
(2022)
Article
Cardiac & Cardiovascular Systems
Gan Lu, Demin Liu, Dina Xie, Wayne Bond Lau, Jing Liu, Theodore A. Christopher, Bernard Lopez, Lian Liu, Hang Hu, Peng Yao, Yarong He, Erhe Gao, Walter J. Koch, Jianli Zhao, Xin-Liang Ma, Yu Cao, Yajing Wang
Summary: Acute myocardial infarction causes endoplasmic reticulum stress and endocrine dysfunction in adipocytes, possibly mediated by the release of small extracellular vesicles enriched with the miR-23-27-24 cluster, to communicate with cardiomyocytes.
CIRCULATION RESEARCH
(2022)
Article
Biotechnology & Applied Microbiology
Rui Zhang, Shuai Niu, Zhihua Rong, Fengshi Li, Leng Ni, Xiao Di, Changwei Liu
Summary: Endothelial dysfunction in diabetic cardiovascular complications can be exacerbated by extracellular vesicles (EVs) derived from high glucose-induced monocytes, which transport miR-142-5p and impair endothelial cells. Inhibiting miR-142-5p may offer potential adjunctive protection for diabetic cardiovascular health.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Physical
Asako Shimoda, Risako Miura, Hiroaki Tateno, Naohiro Seo, Hiroshi Shiku, Shin-ichi Sawada, Yoshihiro Sasaki, Kazunari Akiyoshi
Summary: The study found both similarities and differences in glycan patterns on EVs obtained under different experimental conditions. EV size and isolation method impact lectin-binding patterns. Additionally, cellular uptake behaviors of EVs are influenced by EV glycan profiles and acceptor cells.
Review
Oncology
Md. Khirul Islam, Misba Khan, Kamlesh Gidwani, Kenneth W. Witwer, Urpo Lamminmaeki, Janne Leivo
Summary: Extracellular vesicles (EVs) have potential as diagnostic, prognostic, and therapeutic agents for cancer. The glycosylation patterns on the EV surface can reveal their cell of origin and be used for targeted delivery. Lectins and other glycan recognizing entities can be powerful tools for discovering and detecting novel cancer biomarkers.
BIOMARKER RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Giulia Furesi, Antonio Miguel de Jesus Domingues, Dimitra Alexopoulou, Andreas Dahl, Matthias Hackl, Johannes R. Schmidt, Stefan Kalkhof, Thomas Kurth, Hanna Taipaleenmaeki, Stefanie Conrad, Christine Hofbauer, Martina Rauner, Lorenz C. Hofbauer
Summary: Prostate cancer communicates with bone progenitor cells through extracellular vesicles, promoting vitality and proliferation of the progenitor cells while decreasing mineral deposition. Additionally, small RNAs released by prostate cancer cells may play a critical role in suppressing the activity of bone progenitor cells. These findings reveal an important communication mechanism between prostate cancer and bone.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Immunology
Chiara Tersigni, Donatella Lucchetti, Rita Franco, Filomena Colella, Caterina Neri, Laura Crispino, Alessandro Sgambato, Antonio Lanzone, Giovanni Scambia, Manu Vatish, Nicoletta Di Simone
Summary: PE is a common pregnancy disorder characterized by hypertension and proteinuria, with aberrant expression of HLA-DR in STBEVs specifically associated with PE. Further studies are needed to define the role of abnormal placental HLA-DR expression in PE pathogenesis and evaluate the potential application of detecting circulating HLA-DR positive STBEVs in diagnosing and predicting PE in early and mid-pregnancy.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Xianhui Ruan, Minghui Cao, Wei Yan, Ying Z. Jones, Asa B. Gustafsson, Hemal H. Patel, Simon Schenk, Shizhen Emily Wang
Summary: Extracellular vesicles secreted by breast cancer cells impair skeletal muscle mitochondrial homeostasis and function through the transfer of miR-122-5p to myocytes, leading to decreased mitochondrial content and energy production and increased oxidative stress. Restoring Tp53 in muscle can abolish mitochondrial myopathology and partially rescue impaired running capacity in mice carrying breast tumors. These findings suggest that breast cancer cell-derived extracellular vesicles mediate skeletal muscle mitochondrial dysfunction, contributing to muscle weakness in cancer patients.
Article
Nanoscience & Nanotechnology
Valentina Fokina, Svetlana Patrikeeva, Xiaoming Wang, Mansi Shah, Poonam Shah, William K. Russell, Mahmoud S. Ahmed, Erik Rytting, Tatiana Nanovskaya
Summary: The purpose of this study was to characterize membrane vesicles obtained from human placenta and explore their potential as biological vehicles for drug delivery. The vesicles showed stability, low toxicity, and low immunogenicity, making them suitable for further development. Additionally, the vesicles were found to distribute to various organs within 24 hours of administration. These findings suggest that placental membrane vesicles have promising applications in drug delivery.
JOURNAL OF BIOMEDICAL NANOTECHNOLOGY
(2022)
Article
Endocrinology & Metabolism
Yuehan Li, Lei Cai, Na Guo, Chang Liu, Meng Wang, Lixia Zhu, Fei Li, Lei Jin, Cong Sui
Summary: This study aimed to investigate the influence of oviductal extracellular vesicles (oEV) from patients with endometriosis on early embryo development. In vitro experiments were conducted using human oEV and murine embryos. The results showed that oEV from endometriosis patients significantly decreased blastocyst rates and down-regulated oxidative phosphorylation in blastocysts. Additionally, embryos cultured with oEV from endometriosis patients exhibited increased oxidative stress and apoptosis levels. Overall, oEV from patients with endometriosis negatively affect early embryo development by interfering with oxidative phosphorylation.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Cell Biology
Meredith Whitehead, Syabira Yusoff, Sadia Ahmad, Lukas Schmidt, Manuel Mayr, Jillian Madine, David Middleton, Catherine M. Shanahan
Summary: Vascular amyloidosis, caused by the aggregation of peptide monomers into insoluble amyloid, is a common age-related pathology. This study found that extracellular vesicles (EVs) and HSPG2 in the extracellular matrix (ECM) play key roles in medin accumulation, contributing to the development of age-associated aortic medial amyloid (AMA).
Article
Cell Biology
Wenyi Zheng, Rui He, Xiuming Liang, Samantha Roudi, Jeremy Bost, Coly Pierre-Michael, Guillaume van Niel, Samir E. L. Andaloussi
Summary: This study presents a reconfigurable glycoengineering strategy to produce extracellular vesicles (EVs) with strong cellular specificity by endogenously displaying glycans of interest on the EV surface. The strategy showcases the surface display of different glycan ligands on EVs and achieves high specificity towards specific cell types, leading to enhanced therapeutic effects and attenuation of cell damage.
JOURNAL OF EXTRACELLULAR VESICLES
(2022)
Article
Chemistry, Multidisciplinary
Yumei Cao, Jianzhao Ruan, Jingliang Kang, Xiaoyu Nie, Weiren Lan, Guangfeng Ruan, Jia Li, Zhaohua Zhu, Weiyu Han, Su'an Tang, Changhai Ding
Summary: Infrapatellar fat pad (IPFP) is associated with the progression of knee osteoarthritis (OA), as it secretes small extracellular vesicles (sEVs) that promote cartilage destruction and cellular senescence. The let-7b-5p and let-7c-5p in IPFP-sEVs directly decrease the negative regulator of senescence, lamin B receptor (LBR). This study uncovers the function and mechanism of IPFP-sEVs in the progression of OA, providing a potential strategy for OA therapy.
Review
Cell Biology
Tiantian Li, Hongchi Yu, Demao Zhang, Tang Feng, Michael Miao, Jianwei Li, Xiaoheng Liu
Summary: Vascular calcification is associated with increased risk of heart disease, stroke, and atherosclerotic plaque rupture. Extracellular vesicles, particularly matrix vesicles, play a crucial role in the early stages of vascular calcification. However, the underlying mechanism by which matrix vesicles drive vascular smooth muscle cells phenotype switching and calcification remains unclear.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Geriatrics & Gerontology
Luis Alfonso Gonzalez-Molina, Juan Villar-Vesga, Julian Henao-Restrepo, Andres Villegas, Francisco Lopera, Gloria Patricia Cardona-Gomez, Rafael Posada-Duque
Summary: Research indicates that EVs released by astrocytes in Alzheimer's disease (AD) affect neuroglial and vascular components, leading to cell death and neurotoxicity.
FRONTIERS IN AGING NEUROSCIENCE
(2021)
Review
Public, Environmental & Occupational Health
Ayah Abdul-Hussein, Ayesha Kareem, Shrankhala Tewari, Julie Bergeron, Laurent Briollais, John R. G. Challis, Sandra T. Davidge, Claudio Delrieux, Isabel Fortier, Daniel Goldowitz, Pablo Nepomnaschy, Ashley Wazana, Kristin L. Connor
Summary: This study conducted a Rapid Evidence Review within the DOHaD framework, finding that the majority of DOHaD research data come from high-income countries, with studies examining social determinants of health and paternal influences being underrepresented. Only 23% of the articles explored resiliency factors.
JOURNAL OF DEVELOPMENTAL ORIGINS OF HEALTH AND DISEASE
(2021)
Article
Biochemistry & Molecular Biology
Esha Ganguly, Raven Kirschenman, Floor Spaans, Claudia D. Holody, Thomas E. J. Phillips, C. Patrick Case, Christy-Lynn M. Cooke, Michael P. Murphy, Helene Lemieux, Sandra T. Davidge
Summary: Complications associated with prenatal hypoxia can lead to increased placental oxidative stress, impacting fetal development. A placenta-targeted treatment strategy using nMitoQ was found to improve placental mitochondrial function in both male and female placentae exposed to prenatal hypoxia. This strategy could potentially enhance placental function in complicated pregnancies by reducing oxidative stress.
Article
Physiology
Laura Marcela Reyes, Charlotte W. Usselman, Rshmi Khurana, Radha S. Chari, Michael K. Stickland, Sandra T. Davidge, Colleen G. Julian, Craig D. Steinback, Margie H. Davenport
Summary: This study found that women with preeclampsia had higher baseline mean arterial pressure, noradrenaline concentrations, and MSNA occurrence probability compared to controls, but no significant difference in baseline MSNA levels between the two groups. The chemoreflex does not contribute to increased MSNA in women with preeclampsia.
JOURNAL OF APPLIED PHYSIOLOGY
(2021)
Article
Pharmacology & Pharmacy
Nataliia Hula, Floor Spaans, Jennie Vu, Anita Quon, Raven Kirschenman, Christy-Lynn M. Cooke, Tom J. Phillips, C. Patrick Case, Sandra T. Davidge
Summary: Offspring born from pregnancies complicated by prenatal hypoxia are at higher risk of cardiovascular disease in adulthood. Cardiac tolerance to ischemia/reperfusion (I/R) insult is decreased in both male and female offspring exposed to hypoxia during fetal life, but can be improved by maternal treatment with nMitoQ. The molecular mechanisms underlying the improved cardiac tolerance to I/R may involve changes in the levels/phosphorylation of proteins important for intracellular Ca2+ cycling.
PHARMACOLOGICAL RESEARCH
(2021)
Article
Medicine, General & Internal
Kalyanaraman Kumaran, Ghattu Krishnaveni, Kumar Gavali Suryanarayana, Manohar Prabhu Prasad, Antonisamy Belavendra, Stephanie Atkinson, Ramaswamy Balasubramaniam, Robert H. J. Bandsma, Zulfiqar A. Bhutta, Giriraj Ratan Chandak, Elena M. Comelli, Sandra T. Davidge, Cindy-Lee Dennis, Geoffrey L. Hammond, Prabhat Jha, K. S. Joseph, Sadhana R. Joshi, Murali Krishna, Kang Lee, Stephen Lye, Patrick McGowan, Pablo Nepomnaschy, Vivek Padvetnaya, Saumyadipta Pyne, Harshpal Singh Sachdev, Sirazul Ameen Sahariah, Nalini Singhal, Jacquetta Trasler, Chittaranjan S. Yajnik, Janis Baird, Mary Barker, Marie-Claude Martin, Nusrat Husain, Daniel Sellen, Caroline H. D. Fall, Prakesh S. Shah, Stephen G. Matthews
Summary: The study protocol describes a comprehensive intervention to reduce non-communicable disease risks in children through a village-based cluster randomised design in rural India. The multifaceted intervention includes measures to optimize nutrition, parenting programme, lifestyle behavior change intervention, and environmental pollution reduction. The study will collect various biospecimens to assess intervention effects and conduct intention-to-treat analysis and process and economic evaluations.
Review
Biochemistry & Molecular Biology
Jamie Strain, Floor Spaans, Mohamed Serhan, Sandra T. Davidge, Kristin L. Connor
Summary: Exposome research aims to understand the various environmental exposures that impact human health, particularly the mechanism behind obesity. Maternal metabolic exposome and early environmental exposures increase the risk of offspring obesity through mechanisms such as epigenetic modifications, altered placental function, changes in gut microbiome and breast milk composition, and metabolic inflammation affecting development of the central appetite system, adipose tissues, and liver.
MOLECULAR ASPECTS OF MEDICINE
(2022)
Article
Physiology
Mazhar Pasha, Amy L. Wooldridge, Raven Kirschenman, Floor Spaans, Sandra T. Davidge, Christy-Lynn M. Cooke
Summary: The study found that advanced maternal age is associated with heightened blood pressure in non-pregnant rats, and different responses in endothelium-dependent relaxation to vasodilators and big endothelin-1, indicating compensatory mechanisms in vascular function in aged rats.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Public, Environmental & Occupational Health
Amy L. Wooldridge, Nataliia Hula, Raven Kirschenman, Floor Spaans, Christy-Lynn M. Cooke, Sandra T. Davidge
Summary: Prenatal hypoxia adversely affects pregnancy adaptations and outcomes in adult offspring.
JOURNAL OF DEVELOPMENTAL ORIGINS OF HEALTH AND DISEASE
(2022)
Article
Biochemistry & Molecular Biology
Amy L. Wooldridge, Mazhar Pasha, Palehswan Chitrakar, Raven Kirschenman, Anita Quon, Floor Spaans, Tamara Saez, Christy-Lynn M. Cooke, Sandra T. Davidge
Summary: The study found that advanced maternal age is associated with pregnancy complications and impairs uterine artery adaptations to pregnancy. Aged dams had worse pregnancy outcomes, with altered myogenic behavior and vascular structure in their arteries during pregnancy. These changes may affect fetal growth and development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Mazhar Pasha, Raven Kirschenman, Amy Wooldridge, Floor Spaans, Christy-Lynn M. Cooke, Sandra T. Davidge
Summary: The study indicates that advanced maternal age is associated with endoplasmic reticulum (ER) stress, and the ER stress inhibitor TUDCA may improve pregnancy outcomes.
Article
Biochemistry & Molecular Biology
Nataliia Hula, Ricky Liu, Floor Spaans, Mazhar Pasha, Anita Quon, Raven Kirschenman, Christy-Lynn M. Cooke, Sandra T. T. Davidge
Summary: Prenatal hypoxia predisposes offspring to cardiovascular dysfunction in adulthood, impairing endothelium-dependent vasodilation and increasing PGHS-dependent vasoconstriction. Additionally, prenatal hypoxia enhances the contribution of endothelium-dependent hyperpolarization to coronary artery vasodilation.
Article
Peripheral Vascular Disease
Roberto Villalobos-Labra, Ricky Liu, Floor Spaans, Tamara Saez, Tamara Semeria Maitret, Anita Quon, Tatsuya Sawamura, Christy-Lynn M. Cooke, Sandra T. Davidge
Summary: The study demonstrates that LOX-1 mediates the endothelial dysfunction induced by preeclampsia-STBEVs. This finding expands on the mechanisms that may lead to adverse outcomes in preeclampsia and proposes LOX-1 as a potential target for future interventions.
Article
Biochemistry & Molecular Biology
Paulami Chatterjee, Claudia D. Holody, Raven Kirschenman, Murilo E. Graton, Floor Spaans, Tom J. Phillips, C. Patrick Case, Stephane L. Bourque, Helene Lemieux, Sandra T. Davidge
Summary: Prenatal hypoxia impairs cardiac mitochondrial function in adult female offspring, but this can be improved with prenatal treatment using a nanoparticle-encapsulated mitochondrial antioxidant. The study found that prenatal hypoxia did not affect cardiac mitochondrial function in male offspring, while it decreased the capacity of certain mitochondrial pathways in females. However, treatment with nMitoQ increased respiratory coupling efficiency in female offspring exposed to prenatal hypoxia. This suggests that targeting placental oxidative stress in prenatal hypoxia may reduce the risk of cardiovascular disease in adult offspring by improving cardiac mitochondrial function, specifically in females.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)