Article
Agriculture, Dairy & Animal Science
Maria Moessler, Kathrin Rychli, Volker Michael Reichmann, Thiemo Albert, Thomas Wittek
Summary: In this study, the concentration of immunoglobulin G (IgG) in alpaca colostrum was determined and its correlations with fat, protein, lactose, and minerals were calculated. The results showed that the IgG concentration decreased significantly within the first four days after parturition. There were negative correlations between IgG and fat/lactose content, but strong positive correlations with protein, magnesium, and cobalt content. The protein concentration of colostrum could be used to estimate the IgG content.
Article
Biotechnology & Applied Microbiology
Mais Sweiss, Sulaf Assi, Lina Barhoumi, Dhiya Al-Jumeily, Megan Watson, Megan Wilson, Tom Arnot, Rod Scott
Summary: The portable ATR-FTIR spectroscopy provides a rapid and accurate method for monitoring the biochemical composition of microalgal biomass during different growth stages. Machine learning analytics can differentiate between microalgae isolates based on their species. Univariate or multivariate regression can accurately quantify key microalgal constituents. Carbohydrates are more accurately quantified using multivariate methods, while proteins and lipids are more accurately quantified using univariate regression.
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
(2023)
Article
Chemistry, Analytical
Cecilia Rossi, Alfonso Zoleo, Renzo Bertoncello, Moreno Meneghetti, Rita Deiana
Summary: Illuminated manuscripts are the result of a complex interaction of different skills, reflecting historical and geographical traditions in their uses and techniques. Defining the characteristics and materials of these artifacts is essential for reconstructing their history and facilitating comparison with other works. Non-invasive methods, particularly using portable instruments such as multispectral imaging and spectroscopic measurements, offer valuable support for in-depth understanding of the techniques and practices behind the creation of illuminated manuscripts.
Article
Plant Sciences
Clifton G. Scarboro, Stephanie M. Ruzsa, Colleen J. Doherty, Michael W. Kudenov
Summary: In this study, a bispectral imaging system was developed to differentiate between leaf regions infected with gray mold disease and unharmed areas, aiding in crop management. By selecting two optimal spectral bands and combining resulting images, a high contrast between infected and healthy regions was achieved. The system showed a true-positive rate of 95.25% and a false-positive rate of 9.316% in laboratory conditions.
Article
Chemistry, Applied
Cassius E. O. Coombs, Robert R. Liddle, Luciano A. Gonzalez
Summary: The study successfully differentiated between grass-fed and grain-fed beef using portable NIR and Raman spectrometers, with higher accuracy in fat samples than lean samples for both spectral techniques. Predictions for days on feed and marbling using these techniques were less precise for lean samples compared to fat samples.
JOURNAL OF NEAR INFRARED SPECTROSCOPY
(2021)
Article
Agriculture, Multidisciplinary
Aimen El Orche, Omar Elhamdaoui, Amine Cheikh, Brahim Zoukeni, Miloud El Karbane, Mohamed Mbarki, Mustapha Bouatia
Summary: The study established and validated three multivariate approaches for accurate detection and reliable assessment of the authenticity of argan oil, achieving the rapid identification of counterfeit argan oil.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2022)
Article
Medicine, Research & Experimental
Jiatong Chai, Zeyu Sun, Shunli Zhang, Qi Zhou, Jiancheng Xu
Summary: This study established reference intervals for immunoglobulins using different methods and found that sex and age have an impact on immunoglobulins G and M. The reference intervals established by the four methods all passed verification, providing a methodological reference for further research and benefiting the application of clinical data.
SCANDINAVIAN JOURNAL OF CLINICAL & LABORATORY INVESTIGATION
(2022)
Article
Environmental Sciences
Sara Knezevic, Jelena Ostojic, Milos Ognjanovic, Aleksandra Kovacevic, Dragan Manojlovic, Vesna Stankovic, Dalibor Stankovic
Summary: This study successfully detected triclosan (TCS) using a double approach and proposed two different electrochemical platforms. The results obtained from laboratory analysis and on-site application were satisfactory. Furthermore, the study demonstrated that the influence of potential interferents could be neglected.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Review
Pediatrics
Lauren E. H. Westenberg, Jasper V. Been, Sten P. Willemsen, Jolande Y. Vis, Andrei N. Tintu, Wichor M. Bramer, Peter H. Dijk, Eric A. P. Steegers, Irwin K. M. Reiss, Christian V. Hulzebos
Summary: Importance quantification of bilirubin in blood is crucial for early diagnosis and timely treatment of neonatal hyperbilirubinemia. Handheld point-of-care devices can address the current issues with conventional laboratory-based bilirubin quantification. The evaluation of diagnostic accuracy shows that point-of-care devices have faster turnaround time and require less blood volume compared to laboratory-based quantification, but the precision of measurement needs improvement.
Article
Environmental Sciences
Ziyi Liu, Luning Li, Weiming Xu, Xuesen Xu, Zhicheng Cui, Liangchen Jia, Wenhao Lv, Zhihui Shen, Rong Shu
Summary: As part of China's Tianwen-1 Mars mission, the MarSCoDe instrument on the Zhurong rover uses LIBS to detect the chemical components of materials on the Martian surface. This study investigates the influence of chemometrics and spectral data preprocessing on LIBS quantification accuracy. By collecting 2340 LIBS spectra from 39 geochemical samples in a simulated Martian environment, the study compares the performance of two chemometrics algorithms and evaluates the impact of various preprocessing methods. The results show that using a back-propagation neural network (BPNN) and Mg-peak wavelength correction preprocessing can improve the quantification accuracy.
Article
Pharmacology & Pharmacy
A. M. S. Alnaqbi, B. A. Bhongade, A. Azzawi
Summary: The validated diffuse reflectance infrared Fourier transform spectroscopic method for quantitative analysis of levofloxacin in solid dosage forms provides an eco-friendly approach with precise and accurate results. The linear calibration curve obtained in the study demonstrated high correlation coefficient and the method was found to be suitable for solid state samples. The proposed method showed good precision, accuracy and reproducibility, making it appropriate for quantitative analysis of levofloxacin in solid dosage forms.
INDIAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2021)
Article
Biochemical Research Methods
Eleonora Truzzi, Lucia Marchetti, Davide Bertelli, Stefania Benvenuti
Summary: This study aimed to detect counterfeit essential oils using mid-infrared spectroscopy coupled with chemometrics. The results showed that the developed method was robust in quantifying counterfeits, offering a research advance in phytochemistry and providing an easily applicable method for essential oil quality assessment.
PHYTOCHEMICAL ANALYSIS
(2021)
Article
Soil Science
Michael Vohland, Bernard Ludwig, Michael Seidel, Christopher Hutengs
Summary: The study compared laboratory-based and in situ fusion approaches for visible to near and mid-infrared chemometric modelling in soil studies. It found that regionally stratified approach and model ensemble averaging were beneficial for accurate estimations. The research suggested that simple averaging procedures could advance multi-sensor applications integrating vis-NIR and MIR data for in situ soil spectroscopy, especially in regions with heterogeneous soil conditions.
Article
Medicine, General & Internal
Ajda Mutlu Mihcioglu, Mehmet Bedir Akyol
Summary: Kawasaki disease is an acute febrile multisystem disease that primarily affects children between 6 months and 5 years. Coronary artery involvement is the most serious complication of this disease. This study retrospectively evaluated patients diagnosed and treated for Kawasaki disease at the authors' center.
MEDICAL JOURNAL OF BAKIRKOY
(2023)
Article
Agriculture, Dairy & Animal Science
Christopher B. Riley, Chris W. Rogers, Kirrilly R. Thompson, Danielle Guiver, Barbara Padalino
Summary: This study examines the injuries sustained by horses during road transport in New Zealand and identifies the factors associated with these injuries. The findings highlight the significant incidence of injury and related death during horse transport by road in New Zealand and the key risk factors involved.
Article
Multidisciplinary Sciences
Abdelfattah Selim, Hayat Ali Alafari, Kotb Attia, Muneera D. F. AlKahtani, Fatima M. Albohairy, Ibrahim Elsohaby
Summary: Surra is a parasitic disease caused by Trypanosoma evansi (T. evansi) and transmitted by biting flies. A study in three Egyptian governorates found a high prevalence of T. evansi infection in camels, with older camels, spring season, females, and poor conditioned camels being more susceptible. The card agglutination test for T. evansi showed higher sensitivity than the thin blood smear method and both tests had perfect specificity. The findings suggest that the card agglutination test is a useful tool for the routine control of trypanosomiasis in camels.
SCIENTIFIC REPORTS
(2022)
Article
Agriculture, Dairy & Animal Science
Omid Nekouei, Denis Yau, Brett MacKinnon, Ioannis Magouras, Anne Conan, Ibrahim Elsohaby, Surya Paudel, Dirk U. Pfeiffer
Summary: This study aimed to assess the quality of newly hatched broiler chicks in Hong Kong and provide tailored recommendations for their management. The study found that imported batches generally had higher body weight, length, and antibody levels compared to local batches, while local batches had a higher proportion of abnormal navels. Establishing a well-managed broiler breeder farm and hatchery in Hong Kong is highly recommended to enhance the health and productivity of the local broiler chicken industry.
Article
Agronomy
Wael El-Deeb, Olimpia Iacob, Mahmoud Fayez, Ibrahim Elsohaby, Abdulrahman Alhaider, Hermine Mkrtchyan, Abdelazim Ibrahim, Naser Alhumam
Summary: This study assessed the role of blood biomarkers in calves infected with C. parvum and found that these biomarkers were useful for diagnosis and treatment efficacy.
Article
Veterinary Sciences
Ibrahim Elsohaby, Juan Carlos Arango-Sabogal, Abdelfattah Selim, Kotb A. Attia, Roua A. Alsubki, Arif M. Mohamed, Ameer Megahed
Summary: The objective of this study was to evaluate the diagnostic accuracy of three different tests for detecting Mycobacterium avium subsp. paratuberculosis (MAP) infections in sheep in Egypt. The study found that ELISA had the highest sensitivity among the tests and comparable specificity to the other two tests. ELISA could be a promising alternative for detecting MAP in sheep due to its high sensitivity, cost-effectiveness, and shorter laboratory processing time.
PREVENTIVE VETERINARY MEDICINE
(2022)
Article
Agriculture, Dairy & Animal Science
Megan Ross, Kathryn Proudfoot, Katrina Merkies, Ibrahim Elsohaby, Molly Mills, Kathleen Macmillan, Shawn Mckenna, Caroline Ritter
Summary: This research investigated horse owner attitudes and experiences related to horse welfare, finding that horse owners believe that outdoor and group housing is better for horse welfare, but there is disagreement on whether the standard of care for horses in those housing types is better than indoor and individual housing. Owner attitudes correspond with their housing decisions and consider various horse welfare aspects.
Article
Infectious Diseases
Mahmoud Fayez, Ahmed Elmoslemany, Ahmad A. Al Romaihi, Abdulfattah Y. Azzawi, Abdullah Almubarak, Ibrahim Elsohaby
Summary: Household cats in Saudi Arabia were found to be potential reservoirs of antimicrobial resistance (AMR), with a high frequency of multidrug resistant (MDR) and extended-spectrum beta-lactamases (ESBL) producing E. coli observed. Risk factors associated with MDR and ESBL-producing E. coli colonization in cats included previous antimicrobial treatment and the presence of a child in the cat's family. These findings raise concerns about the transmission of AMR from cats to humans and highlight the importance of a One Health approach.
Article
Multidisciplinary Sciences
Rasha M. M. Abou Elez, Amira S. A. Attia, Hala M. N. Tolba, Reham G. A. Anter, Ibrahim Elsohaby
Summary: This study aimed to identify the presence of Cryptosporidium in samples collected from domestic pigeons, pigeon fanciers, and drinking water, as well as investigate the antiprotozoal activity of biosynthesized silver nanoparticles (AgNPs) on the viability of isolated Cryptosporidium parvum (C. parvum). Cryptosporidium species were found in 16.4% of the examined samples, with C. parvum being the most common. Cryptosporidium was more frequently detected in domestic pigeons than in pigeon fanciers or drinking water.
SCIENTIFIC REPORTS
(2023)
Article
Agriculture, Dairy & Animal Science
Luca Panizzi, Keren E. Dittmer, Matthieu Vignes, Jennie S. Doucet, Kristene Gedye, Mark R. Waterland, Chris W. Rogers, Hiroki Sano, C. Wayne McIlwraith, Christopher B. Riley
Summary: This study aimed to determine if the measurement of cell-free DNA could be used for the early diagnosis of osteoarthritis in horses. Osteoarthritis was induced in some horses, and joint fluid and plasma samples were collected for measurement. The results showed that the concentration of cell-free DNA in joint fluid was significantly higher in horses with osteoarthritis compared to the control group, but there were no significant differences in plasma cell-free DNA concentrations. Measurement of cell-free DNA in joint fluid may be useful for early diagnosis of osteoarthritis in horses.
Article
Agriculture, Dairy & Animal Science
Christopher B. Riley, Barbara Padalino, Chris W. Rogers, Kirrilly R. Thompson
Summary: There is an increased understanding of shared human-animal risk in terms of one welfare, whereby when animals are at risk, so are people, so preventing injury to one species may also prevent injury to the other. This study aimed to determine the frequency and associated factors of road equine transport-related injuries to humans in New Zealand. The results showed that 10.5% of horse industry participants experienced injuries while preparing, loading, traveling, or unloading horses, with hand injuries being the most common. Wearing helmets and gloves, and adopting strategies to eliminate equine injuries are recommended to reduce the risk of injury to horse handlers during road transport.
Article
Microbiology
Heba A. Ahmed, Ibrahim Elsohaby, Amina M. Elamin, Abeer E. Abd El-Ghafar, Gamilat A. Elsaid, Mervat Elbarbary, Rasha A. Mohsen, Tamer M. El Feky, Rasha M. El Bayomi
Summary: This study investigated the occurrence, pathotypes, virulotypes, genotypes, and antimicrobial resistance patterns of ESBL-producing E. coli in retail meat samples and workers in retail meat shops in Egypt. The results showed that the highest proportion of ESBL-producing E. coli isolates was found in duck meat and workers' hand swabs. Pathotyping revealed that the isolates belonged to groups A and B1. Virulotyping showed that the most prevalent virulence gene was Shiga toxin 2 (stx2) associated gene. Genotyping of the identified isolates from human and meat sources showed 100% similarity within the same cluster. All isolates were classified as MDR with an average MAR index of 0.7. AgNPs-H2O2 at various concentrations showed complete bacterial growth inhibition.
Editorial Material
Veterinary Sciences
Ibrahim Elsohaby, Luca Villa
Summary: Zoonotic diseases, like a game of tag, remind us of our interconnectedness with the animal kingdom and the importance of taking proactive measures to prevent their spread.
BMC VETERINARY RESEARCH
(2023)
Article
Microbiology
Rasha M. M. Abou Elez, Ibrahim Elsohaby, Abdul-Raouf Al-Mohammadi, Marwa Seliem, Asmaa B. M. B. Tahoun, Amira I. Abousaty, Reem M. Algendy, Eman A. A. Mohamed, Nashwa El-Gazzar
Summary: This study identified the presence of Listeria spp., including L. monocytogenes, in various samples in Egypt, and characterized their serotypes, virulence-associated genes, antimicrobial resistance patterns, and biofilm formation. It was found that a significant percentage of the samples were positive for Listeria spp., with L. innocua and L. monocytogenes being the most common species. The L. monocytogenes isolates exhibited virulence-associated genes, multidrug resistance, and strong biofilm-forming abilities. Additionally, the study investigated the potential antibacterial and anti-biofilm effects of a specific strain of Lactobacillus plantarum against L. monocytogenes isolates.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Hend M. El Damaty, Azza S. El-Demerdash, Norhan K. Abd El-Aziz, Sarah G. Yousef, Ahmed A. Hefny, Etab M. Abo M. Remela, Asmaa Shaker, Ibrahim Elsohaby
Summary: Caseous lymphadenitis (CLA) is an infectious disease caused by Corynebacterium pseudotuberculosis in sheep and goats. In this study, C. pseudotuberculosis was isolated and identified from CLA in smallholder sheep and goats. The resistance patterns, virulence genes, and resistance genes of the isolates were determined. Molecular characterization and genotyping analysis revealed the presence of various virulence and resistance genes in the isolates. The findings highlight the importance of implementing control strategies and increasing awareness to mitigate the economic impact of CLA in smallholder farming.
Article
Microbiology
Sara H. Mahrous, Farouk A. El-Balkemy, Naser Z. Abo-Zeid, Mamdouh F. El-Mekkawy, Hend M. El Damaty, Ibrahim Elsohaby
Summary: This study aimed to isolate and identify Klebsiella pneumoniae, determine its antimicrobial resistance patterns and biofilm formation abilities, and investigate the effects of cinnamon oil on the bacteria. The results showed a high level of multidrug-resistant K. pneumoniae with strong and moderate biofilm formation abilities in pneumonic sheep and goats. Cinnamon oil demonstrated potential antibacterial and anti-biofilm properties against K. pneumoniae.