Article
Immunology
Yanping Qin, Weitao Wan, Jiangwei Li, Zhongyu Wang, Yue Yang, Jun Li, Haitao Ma, Ziniu Yu, Zhiming Xiang, Yuehuan Zhang
Summary: This study identified and cloned a novel FasL gene, ChFasL, from the genome of Crassostrea hongkongensis. The results demonstrated the significant regulatory role of ChFasL in marine bivalve, highlighting its importance in understanding the immune function, pathogenic death mechanism, and disease resistance breeding.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Immunology
Paula F. Krieg, Jana K. Sonner, Roberta Kurelic, Jan Broder Engler, Marlena F. Scharenberg, Simone Bauer, Viacheslav O. Nikolaev, Manuel A. Friese
Summary: GPR52 regulates cAMP levels in T cells but does not affect T cell function.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Immunology
Xiaoxue Deng, Lingxia Wang, Yunze Zhai, Qiuyue Liu, Fengxue Du, Yu Zhang, Wenxing Zhao, Tingtao Wu, Yiwen Tao, Jie Deng, Yongbing Cao, Pei Hao, Jiazi Ren, Yunli Shen, Zuoren Yu, Yuejuan Zheng, Haibing Zhang, Haikun Wang
Summary: Regulatory T (T-reg) cells are important for maintaining immune balance, and RIPK1 plays a critical role in regulating T-reg cell survival and homeostasis by controlling both apoptosis and necroptosis mechanisms.
CELLULAR & MOLECULAR IMMUNOLOGY
(2023)
Article
Immunology
Amine Mellouk, Tom Hutteau-Hamel, Julie Legrand, Hanaa Safya, Mohcine Benbijja, Francoise Mercier-Nome, Karim Benihoud, Jean M. Kanellopoulos, Pierre Bobe
Summary: The development of lymphoproliferative and lupus diseases in MRL/lpr mice depends on the interaction between the Fas(lpr) mutation and MRL genetic background. P2X7 receptor defect leads to abnormal accumulation of B220(+) CD4(-)CD8(-) double negative T cells in MRL/lpr mice, and the cooperation between P2X7 and Fas is critical for T-cell homeostasis.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Chaitrali Saha, Jingyu Li, Xuerong Sun, Xinguang Liu, Gonghua Huang
Summary: Fas-mediated apoptosis plays a significant role in various cellular processes. The immune regulation controlled by Fas can have both beneficial and harmful effects on immune disorders. The role of apoptosis in the ageing process is still unclear, but this study shows that Fas signaling can induce premature senescence in mouse embryonic fibroblasts, while Fas deficiency leads to senescence through increased SA-beta-galactosidase activity, p53 phosphorylation, and the expression of p16, p21, and IL-6.
Article
Immunology
Cameron R. Bastow, Ervin E. Kara, Timona S. Tyllis, Carola G. Vinuesa, Shaun R. McColl, Iain Comerford
Summary: Follicular T cells, including T follicular helper (T-FH) and T follicular regulatory (T-FR) cells, play essential roles in supporting and regulating antibody responses. This study found that follicular T cells express not only CXCR5 and CXCR4, but also CCR6, CCR2 and CXCR3. CCR6 is particularly highly expressed in T-FR cells and gating on CCR6(+)CXCR5(hi)PD-1(hi) T cells enriches for T-FR cells. However, CCR6 is not essential for development of the germinal center response and does not affect T-FR cell development and localization during splenic humoral responses.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Noor Mustafa, Jone Mitxelena, Arantza Infante, Olatz Zenarruzabeitia, Ainhoa Eriz, Ainhoa Iglesias-Ara, Ana M. Zubiaga
Summary: Disruption of E2f2 in mice leads to T-cell hyperactivation and disproportionate cell cycle entry. However, E2f2(-/-) mice do not develop lymphoproliferative condition. We find that E2f2 plays an anti-apoptotic role through the Fas pathway in vitro and in vivo. Knockout of E2f2 in TCR-stimulated T cells results in overexpression of proapoptotic genes, enhanced apoptosis, and activated p53 pathway. However, E2f2-Fas/FasL signaling is p53-independent. E2f2 is recruited to Fas and FasL gene promoters to repress their expression. E2f2(-/-) mice are prone to immune-mediated liver injury due to aberrant lymphoid Fas/FasL activation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Clinical Neurology
Suresh L. Mehta, Anil K. Chokkalla, TaeHee Kim, Saivenkateshkomal Bathula, Bharath Chelluboina, Kahlilia C. Morris-Blanco, Aleah Holmes, Anik Banerjee, Anjali Chauhan, Juneyoung Lee, Venugopal R. Venna, Louise D. McCullough, Raghu Vemuganti
Summary: The brain-specific long noncoding RNA called Fos downstream transcript (FosDT) is rapidly induced in the brain following focal ischemia. Knockout rats lacking FosDT show enhanced sensorimotor recovery and reduced brain damage post-stroke. Improved outcomes in FosDT(-/-) rats are associated with reduced inflammation, apoptosis, mitochondrial dysfunction, and oxidative stress.
Article
Immunology
Britta E. Jones, Megan D. Maerz, Henry T. Bahnson, Ashwin Somasundaram, Lucas H. McCarthy, Cate Speake, Jane H. Buckner
Summary: The coinhibitory receptor LAG-3 plays a role in regulating T cell activation and proliferation, and its low expression is associated with altered T cell function and resistance to cell death in subjects with RRMS. Dysregulation of LAG-3 expression persists after T cell stimulation, suggesting its potential as a target for autoimmune disease treatment.
JOURNAL OF IMMUNOLOGY
(2022)
Article
Immunology
Sarah K. Whiteside, Francis M. Grant, David S. Gyori, Alberto G. Conti, Charlotte J. Imianowski, Paula Kuo, Rabab Nasrallah, Firas Sadiyah, Sergio A. Lira, Frank Tacke, Robert L. Eil, Oliver T. Burton, James Dooley, Adrian Liston, Klaus Okkenhaug, Jie Yang, Rahul Roychoudhuri
Summary: CCR8 function is not essential for Treg cell accumulation and immunosuppressive function within tumors, suggesting that depletion of CCR8(+) Treg cells rather than blockade of CCR8 function may be a more promising avenue for selective immunotherapy.
Review
Pharmacology & Pharmacy
Fei Xiong, Qiao Zhou, Xiaobo Huang, Peng Cao, Yi Wang
Summary: This article summarizes the role of ferroptosis in the pathogenesis of nonalcoholic steatohepatitis (NASH) and discusses the relationship between ferroptosis, mitochondrial dysfunction, and NASH. Potential pharmacological therapies targeting ferroptosis in NASH are also proposed.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Jiangtao Ren, Xuhua Zhang, Zhenhui Zhang, Jiafeng Pan, Zhexue Hao, Jin Li, Jun Liu
Summary: The complex and costly manufacturing process of CAR-T cell therapy has hindered its widespread adoption. Induced pluripotent stem cells (iPSCs) have potential as an alternative cellular immunotherapy due to their unlimited self-renewal potential and ability to differentiate into functional immune cells. Careful selection of the original cell for iPSC seed preparation is crucial due to the presence of epigenetic imprint. Our research shows that lymphocyte-origin cells are superior to embryonic stem cells in differentiating into functional immune cells. Blocking Fas-FasL induced apoptosis in T cells significantly improves iPSC generation without affecting their differentiation potential.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Geetika Bassi, Suresh Mishra
Summary: Mitochondria play a crucial role in steroidogenesis, particularly in cholesterol transport and P450scc enzyme synthesis. A study on transgenic mice reveals that PHB1 has a regulatory role in Leydig cell steroidogenesis, involving cell signaling, cholesterol homeostasis, and mitochondrial biology.
Article
Developmental Biology
Dawei Zhu, Jie Huang, Xing Gu, Li Li, Jian Han
Summary: This study revealed that downregulation of aromatase in the placenta of preeclampsia patients is associated with hormonal imbalance and PE-like symptoms in pregnant mice. The decreased CYP19A1 expression enhanced trophoblast cell invasion and migration for placental implantation, while also increasing apoptosis and inflammatory response. Activation of the PI3K/AKT signaling pathway may play a key role in these processes, offering a potential novel method for early prediction and treatment of PE.
MOLECULAR HUMAN REPRODUCTION
(2021)
Review
Biochemistry & Molecular Biology
Shizhen Zhao, Honggang Wang
Summary: EVA1A, a protein associated with regulating autophagy and cell apoptosis, plays significant roles in physiological processes like embryonic neurogenesis and cardiac remodeling. However, the underlying molecular mechanisms require further exploration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Xuhua Zhang, John P. Dowling, Jianke Zhang
CELL DEATH & DISEASE
(2019)
Article
Multidisciplinary Sciences
John P. Dowling, Mohamed Alsabbagh, Christina Del Casale, Zheng-Gang Liu, Jianke Zhang
NATURE COMMUNICATIONS
(2019)
Article
Immunology
Rohan Keshwara, Thomas Shiels, Elena Postnikova, Drishya Kurup, Christoph Wirblich, Reed F. Johnson, Matthias J. Schnell
Article
Immunology
Drishya Kurup, Christine R. Fisher, Todd G. Smith, Tiago Abreu-Mota, Yong Yang, Felix R. Jackson, Nadia Gallardo-Romero, Richard Franka, Victor Bronshtein, Matthias J. Schnell
JOURNAL OF INFECTIOUS DISEASES
(2019)
Article
Immunology
Drishya Kurup, Christoph Wirblich, Holly Ramage, Matthias J. Schnell
Article
Microbiology
Drishya Kurup, Delphine C. Malherbe, Christoph Wirblich, Rachael Lambert, Adam J. Ronk, Leila Zabihi Diba, Alexander Bukreyev, Matthias J. Schnell
Summary: The study developed an inactivated rabies virus vectored vaccine platform, resulting in a vaccine against SARS-CoV-2 named CORAVAX. CORAVAX demonstrated effectiveness in preventing viral replication and lung disease in hamster models, showing potential for robust immune responses in humans.
Article
Immunology
Delphine C. Malherbe, Drishya Kurup, Christoph Wirblich, Adam J. Ronk, Chad Mire, Natalia Kuzmina, Noor Shaik, Sivakumar Periasamy, Matthew A. Hyde, Julie M. Williams, Pei-Yong Shi, Matthias J. Schnell, Alexander Bukreyev
Summary: The study developed a SARS-CoV-2 ConVac vaccine which, through animal experiments, showed that a single dose of the vaccine could rapidly control viral replication after disease challenge, protecting animals from weight loss and lung virus replication, with some inflammation still detected.
Article
Immunology
Jillian M. DiMuzio, Baron C. Heimbach, Raymond J. Howanski, John P. Dowling, Nirja B. Patel, Noeleya Henriquez, Chris Nicolescu, Mitchell Nath, Antonio Polley, Jamie L. Bingaman, Todd Smith, Benjamin C. Harman, Matthew K. Robinson, Michael J. Morin, Pavel A. Nikitin
Summary: A study on the anti-viral memory B cell repertoire of convalescent patients showed that antibodies were produced against multiple viral proteins, with a majority targeting the Spike protein. This study suggests advantages of a function-based approach in identifying highly effective anti-viral antibodies against SARS-CoV-2.
Article
Allergy
Drishya Kurup, Jacob Myers, Matthias J. Schnell
Summary: This review examines the path of vaccine discovery for COVID-19 and discusses challenges and opportunities. It also covers emerging mutations within the SARS-CoV-2 spike protein and other issues that need to be addressed.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2022)
Article
Virology
Drishya Kurup, Christoph Wirblich, Leila Zabihi Diba, Rachael Lambert, Megan Watson, Noor Shaikh, Holly Ramage, Charalambos Solomides, Matthias J. Schnell
Summary: This study reports the immunogenicity and efficacy of an adjuvanted single-dose vaccine called CORAVAX in hamsters. CORAVAX induces high levels of antibody immune response, preventing adverse reactions and diseases caused by virus infection. High levels of immune response to rabies were also observed.
Article
Immunology
Drishya Kurup, Christoph Wirblich, Rachael Lambert, Leila Zabihi Diba, Benjamin E. Leiby, Matthias J. Schnell
Summary: The study showed that a measles vaccine expressing both ZIKV-E and -NS1 provided complete clearance of the virus and full protection for fetuses in a lethal Zika challenge model. NS1 antibodies were found to enhance the protective effects of ZIKV-E antibodies in the female reproductive tract.
Article
Immunology
Pavel A. Nikitin, Jillian M. DiMuzio, John P. Dowling, Nirja B. Patel, Jamie L. Bingaman-Steele, Baron C. Heimbach, Noeleya Henriquez, Chris Nicolescu, Antonio Polley, Eden L. Sikorski, Raymond J. Howanski, Mitchell Nath, Halley Shukla, Suzanne M. Scheaffer, James P. Finn, Li-Fang Liang, Todd Smith, Nadia Storm, Lindsay G. A. McKay, Rebecca Johnson, Lauren E. Malsick, Anna N. Honko, Anthony Griffiths, Michael S. Diamond, Purnanand Sarma, Dennis H. Geising, Michael J. Morin, Matthew K. Robinson
Summary: Through the study of the B-cell repertoire of recovered COVID-19 patients, we identified human antibodies that can neutralize various variants of SARS-CoV-2. These antibodies form a cocktail that can effectively inhibit viral infection in vitro and in vivo, and induce antiviral responses in the body.
SCIENCE IMMUNOLOGY
(2022)
Article
Immunology
Catherine Yankowski, Christoph Wirblich, Drishya Kurup, Matthias J. Schnell
Summary: The objective of this study is to analyze the effectiveness of the recombinant rabies virus-vectored SARS-CoV-2 vaccine, CORAVAX, as a COVID-19 vaccine strategy. The study found that CORAVAX is immunogenic and protective against SARS-CoV-2, and it addresses the issue of waning immunity seen in other COVID-19 vaccines. By selecting the right adjuvants and tackling pre-existing rabies-vector immunity, CORAVAX has shown long-term potential as a COVID-19 vaccine.
Article
Immunology
Catherine Yankowski, Drishya Kurup, Christoph Wirblich, Matthias J. Schnell
Summary: Ebola virus is a major cause of severe hemorrhagic fever with a high mortality rate. An Ebola virus glycoprotein expressing inactivated bivalent filovirus/rabies virus vaccine called FILORAB1 has shown immunogenicity and protection against Ebola virus in mice and non-human primates, especially when formulated with toll-like receptor 4 agonist GLA and squalene oil-in-water emulsion. The immune response to FILORAB1 is long-lasting and characterized by maintained serum antibody titers and long-lived cells in the spleen and bone marrow.
Article
Immunology
Drishya Kurup, Christine R. Fisher, Gabrielle Scher, Catherine Yankowski, AnnaMarie Testa, Rohan Keshwara, Tiago Abreu-Mota, Rachael Lambert, Melissa Ferguson, William Rinaldi, Leonard Ruiz, Christoph Wirblich, Matthias J. Schnell
Summary: The study evaluated the immunogenicity of adjuvanted monovalent rabies virus-based vaccine candidates against filoviruses and Lassa virus in nonhuman primates. Results showed strong and lasting immune responses up to 365 days, with no significant differences between single vaccines and trivalent or tetravalent vaccines. The study demonstrates the potential of a pentavalent vaccine platform against multiple viruses.
JOURNAL OF INFECTIOUS DISEASES
(2021)