Article
Biochemistry & Molecular Biology
Bruna Tereza Thomazini Zanella, Isabele Cristina Magiore, Bruno Oliveira Silva Duran, Guilherme Gutierrez Pereira, Igor Simoes Tiagua Vicente, Pedro Luiz Pucci Figueiredo Carvalho, Rondinelle Artur Simoes Salomao, Edson Assuncao Mareco, Robson Francisco Carvalho, Tassiana Gutierrez de Paula, Margarida Maria Barros, Maeli Dal-Pai-Silva
Summary: The study found that the antioxidant ascorbic acid plays a role in muscle recovery in fish, promoting muscle growth and gene expression. In in vivo experiments, high doses of ascorbic acid can increase muscle cell diameter and the expression of related genes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Bruno E. A. Fantinatti, Erika S. Perez, Bruna T. T. Zanella, Jessica S. Valente, Tassiana G. de Paula, Edson A. Mareco, Robson F. Carvalho, Silvano Piazza, Michela A. Denti, Maeli Dal-Pai-Silva
Summary: Our study revealed differential miRNA expression in the hybrid tambacu, identifying miRNAs controlling important molecular muscle markers that could be relevant for maximizing farming efficiency.
Review
Biochemistry & Molecular Biology
Erika Stefani Perez, Bruno Oliveira Silva Duran, Bruna Tereza Thomazini Zanella, Maeli Dal-Pai-Silva
Summary: The muscle phenotype of fish is regulated by various factors, and this study focuses on the research of fish biology and skeletal muscle growth, particularly using the pacu as an experimental model. New regulators of muscle phenotype have been identified through in vivo, in vitro, and in silico studies, contributing to a better understanding of muscle growth mechanisms and potential applications in the productive sector.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Edson Assuncao Mareco, Daniel Garcia de la Serrana, Tassiana Gutierrez de Paula, Bruna Tereza Thomazini Zanella, Bruno Oliveira da Silva Duran, Rondinelle Artur Simoes Salomao, Bruno Evaristo de Almeida Fantinatti, Victor Hugo Garcia de Oliveira, Vander Bruno dos Santos, Robson Francisco Carvalho, Maeli Dal-Pai-Silva
Summary: Using RNA-Seq analysis, we characterized the transcriptome of white muscle from Pacu, Tambaqui, and their interspecific hybrid (Tambacu). Comparative analysis revealed a higher number of expressed genes in the hybrid genotype. We also identified target genes related to cellular processes through differential expression analysis and validated their expression using RT-qPCR.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS
(2023)
Article
Fisheries
Gabriel Cardoso Neves, Liliane Soares Presa, Lucas Campos Maltez, Jose Maria Monserrat, Luciano Garcia
Summary: This study found that under low hardness conditions, NO2 exposure increased lipid peroxidation levels in muscles and gills, affecting food conversion rate; while under higher hardness conditions, lipid peroxidation levels remained stable, with no significant changes in zootechnical parameters. The addition of CaCO3 to increase calcium levels can reduce the NO2 toxicity levels in pacu juveniles, showing potential for application in water quality management.
Article
Agriculture, Dairy & Animal Science
Andressa Tellechea Rodrigues, Cleber Fernando Menegasso Mansano, Kifayat Ullah Khan, Thiago Matias Torres do Nascimento, Monique Viraes Barbosa dos Santos, Nilva Kazue Sakomura, Joao Batista Kochenborger Fernandes
Summary: This study aimed to estimate the ideal pattern of dietary essential amino acids (EAAs) for pacu in the later-juvenile growth phase. The fish fed with the control diet showed better growth performance and feed utilization. The estimated ideal pattern of dietary EAAs for pacu in this phase was determined.
ANIMAL FEED SCIENCE AND TECHNOLOGY
(2022)
Article
Fisheries
Dara Cristina Pires, Gabriel Artur Bezerra, Andre Luiz Watanabe, Celso Carlos Buglione Neto, Alvaro Jose de Almeida Bicudo, Hamilton Hisano
Summary: This study evaluated the effects of dietary protein levels on various aspects of pacu juveniles, including growth performance, hematological and biochemical parameters, carcass and fillet yields, somatic indexes, and body chemical composition. The results showed that increasing protein levels had a quadratic effect on weight gain, thermal growth coefficient, protein productive value, and fillet yield. Feed consumption and carcass yield increased linearly, while protein efficiency ratio decreased linearly. Blood parameters remained within the normal range for this species. The optimal dietary protein level for the best growth of pacu juveniles reared in the biofloc system was estimated to be approximately 310 g kg(-1) CP.
Article
Biochemistry & Molecular Biology
Bruno Oliveira Silva Duran, Bruna Tereza Thomazini Zanella, Erika Stefani Perez, Edson Assuncao Mareco, Josefina Blasco, Maeli Dal-Pai-Silva, Daniel Garcia de la Serrana
Summary: Amino acids and IGF1 play different roles in regulating gene expression in pacu-cultured myotubes, with potential molecular markers identified. Muscle miRNAs were up-regulated especially in AA samples, revealing various potential molecular networks through omics.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Fisheries
William Franco Carneiro, Tassia Flavia Dias Castro, Tharyn Reichel, Pedro Leite de Castro Uzeda, Carlos Antonio Martinez-Palacios, Luis David Solis Murgas
Summary: Ammonia is a common pollutant in intensive aquaculture systems that can harm animal health and cause economic losses. Diets supplemented with additives like spirulina meal have shown promising results in improving growth performance, immune system, and antioxidant capacity in fishes. Replacing fish meal with spirulina meal at certain levels can improve growth performance and mitigate the adverse effects of ammonia toxicity in pacu juveniles by enhancing antioxidant responses.
Article
Fisheries
Thais S. Oliveira, Kifayat U. Khan, Andre Z. Boaratti, Andressa T. Rodrigues, Matheus P. Reis, Nilva K. Sakomura, Joao B. K. Fernandes
Summary: This study aimed to determine the optimum dietary EAA pattern for adult pacu through the amino acid deletion method. The results showed that certain EAA limiting diets had an impact on fish body nitrogen retention and weight gain.
Article
Fisheries
Cristiane Meldau de Campos, Fabio Sabaddin Zanuzzo, Rodrigo Yukihiro Gimbo, Gisele Cristina Favero, Michelly Pereira Soares, Fabiana Pilarski, Elisabeth Criscuolo Urbinati
Summary: Inulin supplementation in juvenile pacu fish was found to reduce stress response and enhance innate immune responses, potentially improving the health of farmed fish. This study demonstrated the positive effects of inulin on fish immunity and stress management.
AQUACULTURE RESEARCH
(2022)
Article
Agriculture, Dairy & Animal Science
Carolina Vasconcelos Tavares de Farias, Amanda Miyuki Oshiro, Luana Camargo Sousa, Viviane do Nascimento Santana de Almeida, Leonardo Susumu Takahashi
Summary: The study found that pacu fed with 0/390 and 70/320 diets showed higher final body weight, specific growth rate, and protein efficiency ratio. Additionally, diets with amylose contents higher than 200 g/kg resulted in significantly higher crude lipid content in the whole body and muscle lipid, as well as hepatosomatic index compared to the 0/390 diet. It can be concluded that diets with more than 300 g/kg amylopectin and less than 100 g/kg amylose lead to increased starch utilization and growth in pacu.
ANIMAL FEED SCIENCE AND TECHNOLOGY
(2021)
Article
Fisheries
Daniel de Sa Britto Pinto, Lucas Pellegrin, Lilian Fiori Nitz, Jose Maria Monserrat, Luciano Garcia
Summary: The aim of this study was to investigate the effect of temperature on blood parameters, reactive oxygen species, and lipid peroxidation in pacu. The results showed that pacu can be reared in temperatures between 24 and 27 degrees Celsius, with 24.09 degrees Celsius being the optimal temperature for minimizing muscle lipid peroxidation.
AQUACULTURE INTERNATIONAL
(2022)
Article
Fisheries
Milena Freitas, Lieschen V. G. Lira, Raquel B. Ariede, John F. G. Agudelo, Rubens Ricardo de Oliveira Neto, Carolina H. S. Borges, Vito A. Mastrochirico-Filho, Baltasar F. Garcia Neto, Roberto Carvalheiro, Diogo T. Hashimoto
Summary: The study estimated genetic parameters, evaluated genotype by environment interaction, and identified morphotypes for growth traits in pacu using data from a pedigreed population in Brazil. The results showed moderate to high heritability estimates, positive genetic correlations between final weight and morphometric traits, and different morphotype patterns for pacu. GxE interaction was considered low for all traits between the two environments.
Article
Biochemistry & Molecular Biology
Iuri Moraes Neyrao, Jaqueline Dalbello Biller, Leonardo Susumu Takahashi, Elisabeth Criscuolo Urbinati
Summary: The study investigated the effects of oral hydrocortisone and acute stressor on stress, immune responses, and antioxidant system responses in juvenile Piaractus mesopotamicus. The findings showed that exposure to stressor and HC impacted circulating glucose, cortisol levels, and immune responses, while differences in biomarkers indicated that exogenous hormone was unable to precisely replicate stress responses.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Bruno Oliveira Silva Duran, Bruna Tereza Thomazini Zanella, Erika Stefani Perez, Edson Assuncao Mareco, Josefina Blasco, Maeli Dal-Pai-Silva, Daniel Garcia de la Serrana
Summary: Amino acids and IGF1 play different roles in regulating gene expression in pacu-cultured myotubes, with potential molecular markers identified. Muscle miRNAs were up-regulated especially in AA samples, revealing various potential molecular networks through omics.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Genetics & Heredity
Luiz Gustavo de Almeida Chuffa, Paula Paccielli Freire, Jeferson dos Santos Souza, Mariana Costa de Mello, Mario de Oliveira Neto, Robson Francisco Carvalho
Summary: The study revealed that genes such as FASLG, CTSW, CTSE, VCAM1, and BAG3 are upregulated in aged blood and associated with immune response, inflammation, and blood clots. These findings suggest that certain blood molecules may be related to the severe progression of COVID-19.
JOURNAL OF MOLECULAR MEDICINE-JMM
(2022)
Article
Veterinary Sciences
Leticia de Andrade Ramos, Claudia Valeria Seullner Brandao, Carlos Guillermo Arce, Renata Haddad Pinho, Lenise Garbelotti Goncalves, Carlos Roberto Padovani
Summary: The corneal topography and tomography of cats were evaluated using a dual Scheimpflug and Placido disc device, which allowed for detailed examination of the cornea.
VETERINARY OPHTHALMOLOGY
(2022)
Article
Veterinary Sciences
Bruno Martinucci, Maira Smaniotto Cucielo, Brenda Carvalho Minatel, Sarah Santiloni Cury, Gabriel Henrique Caxali, Mirian Carolini Esgoti Aal, Sergio Luis Felisbino, Danillo Pinhal, Robson Francisco Carvalho, Flavia Karina Delella
Summary: This study investigated the impact of fibronectin on miRNA expression in prostate cancer cells and found that these miRNAs may be involved in the development of prostate cancer. This is significant in understanding prostate cancer in both men and other animals.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Fernanda Cristina Bergamo Alves, Rafael Guilen de Oliveira, David Rafael Abreu Reyes, Gabriela Azevedo Garcia, Juliana Ferreira Floriano, Raghavendra Hallur Lakshmana Shetty, Edson Assuncao Mareco, Maeli Dal-Pai-Silva, Spencer Luiz Marques Payao, Fatima Pereira de Souza, Steven S. Witkin, Luis Sobrevia, Angelica Mercia Pascon Barbosa, Marilza Vieira Cunha Rudge
Summary: Gestational diabetes mellitus (GDM) is a window of opportunity for predicting future complications such as type 2 diabetes mellitus and pelvic floor muscle disorders. This study focuses on the transcriptome profiling of the rectus abdominis muscle (RAM) in GDM women and identifies differentially expressed genes associated with muscle structure, muscle protein synthesis, muscle damage repair, and glycogenesis. These findings shed light on the molecular mechanisms of GDM and may lead to innovative interventions for preventing and treating post-GDM urinary incontinence.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Robson Francisco Carvalho, Luisa Matos do Canto, Cecilie Abildgaard, Mads Malik Aagaard, Monica Sogaard Tronhjem, Marianne Waldstrom, Lars Henrik Jensen, Karina Dahl Steffensen, Silvia Regina Rogatto
Summary: This study comprehensively characterizes the crosstalk between cancer-associated fibroblasts (CAFs) and ovarian cancer cells, identifying critical signaling pathways, ligands, and receptors with prognostic and therapeutic significance. Potential therapeutic targets were identified, and genes associated with the PI3K pathway were found to be predictive of overall survival in ovarian cancer.
CELL COMMUNICATION AND SIGNALING
(2022)
Article
Genetics & Heredity
S. S. Cury, J. S. Oliveira, C. A. O. Biagi-Junior, W. A. Silva Jr, P. P. Reis, O. Cabral-Marques, E. N. Hasimoto, P. P. Freire, R. F. Carvalho
Summary: Lung cancer patients with COVID-19 have an increased risk of severe disease and poor outcomes. Analyzing the interactions between SARS-CoV-2 and lung cancer cells may reveal molecular mechanisms associated with COVID-19 development and severity. Transcriptome data analysis showed that lung cancer cells infected with SARS-CoV-2 resembled primary lung tumors more than non-malignant lung tissues. Furthermore, the analysis identified cancer genes BRCA1 and CENPF, which are known or predicted to interact with SARS-CoV-2 proteins, as well as other potential host factors associated with COVID-19 infection and severity.
Article
Agronomy
Elton Luiz Scudeler, Bruno Vinicius Daquila, Shelly Favorito de Carvalho, Helio Conte, Carlos Roberto Padovani, Daniela Carvalho dos Santos
Summary: This study evaluated the effects of chronic exposure to azadirachtin on the morphology and ultrastructure of S. eridania larval testes as well as larval development. The results showed that azadirachtin damaged the testes of S. eridania larvae, compromising spermatogenesis and the reproductive potential of this species, making azadirachtin a promising botanical insecticide for integrated pest management programs.
PEST MANAGEMENT SCIENCE
(2023)
Article
Veterinary Sciences
Leticia de Andrade Ramos, Claudia Valeria Seullner Brandao, Carlos Guillermo Arce, Laura Micheline, Micaella Gordon Gandolfi, Carlos Roberto Padovani
Summary: This study compared the central corneal thickness (CCT) and simulated keratometry (SimK) measured by different devices in cats. The results showed no statistical difference in the CCT and SimK measurements regardless of the device used.
VETERINARY OPHTHALMOLOGY
(2023)
Article
Ophthalmology
Magda Massae Hata Viveiros, Marcia Guimardes Silva, Jose Galberto Martins da Costa, Anselmo Gomes de Oliveira, Carolina Rubio, Carlos Roberto Padovani, Claudia Aparecida Rainho, Silvana Artioli Schellini
Summary: This study aimed to investigate the anti-inflammatory effects of α-humulene and β-caryophyllene on pterygium fibroblasts. The results showed that β-caryophyllene significantly reduced the production of IL-6 by pterygium fibroblasts. Additionally, there were no significant variations in the levels of other inflammatory cytokines after exposure to α-humulene, β-caryophyllene, or both compounds together.
INTERNATIONAL JOURNAL OF OPHTHALMOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Edson Assuncao Mareco, Daniel Garcia de la Serrana, Tassiana Gutierrez de Paula, Bruna Tereza Thomazini Zanella, Bruno Oliveira da Silva Duran, Rondinelle Artur Simoes Salomao, Bruno Evaristo de Almeida Fantinatti, Victor Hugo Garcia de Oliveira, Vander Bruno dos Santos, Robson Francisco Carvalho, Maeli Dal-Pai-Silva
Summary: Using RNA-Seq analysis, we characterized the transcriptome of white muscle from Pacu, Tambaqui, and their interspecific hybrid (Tambacu). Comparative analysis revealed a higher number of expressed genes in the hybrid genotype. We also identified target genes related to cellular processes through differential expression analysis and validated their expression using RT-qPCR.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS
(2023)
Article
Medicine, Research & Experimental
Sarah Santiloni Cury, Diogo de Moraes, Jakeline Santos Oliveira, Paula Paccielli Freire, Patricia Pintor dos Reis, Miguel Luiz Batista Jr, Erica Nishida Hasimoto, Robson Francisco Carvalho
Summary: A prediction model was developed to identify patients with muscle loss in non-small lung cancer. Transcriptome and cellular composition analysis revealed an inflammatory and immunosuppressive tumor microenvironment in patients with low muscle mass.
JOURNAL OF TRANSLATIONAL MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Diogo de Moraes, Felippe Mousovich-Neto, Sarah Santiloni Cury, Jakeline Oliveira, Jeferson dos Santos Souza, Paula Paccielli Freire, Maeli Dal-Pai-Silva, Marcelo Alves da Silva Mori, Geysson Javier Fernandez, Robson Francisco Carvalho
Summary: Aging causes changes in body composition, including an increase in visceral fat mass, which is associated with age-related diseases. Visceral fat can affect systemic metabolism by secreting molecules that act in distal tissues, particularly the liver. However, little is known about age-related changes in visceral fat in humans.
Review
Biochemistry & Molecular Biology
Erika Stefani Perez, Bruno Oliveira Silva Duran, Bruna Tereza Thomazini Zanella, Maeli Dal-Pai-Silva
Summary: The muscle phenotype of fish is regulated by various factors, and this study focuses on the research of fish biology and skeletal muscle growth, particularly using the pacu as an experimental model. New regulators of muscle phenotype have been identified through in vivo, in vitro, and in silico studies, contributing to a better understanding of muscle growth mechanisms and potential applications in the productive sector.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2023)
Article
Reproductive Biology
Vanessa Caroline Fioravante, Alana Rezende Godoi, Victoria Mokarzel de Barros Camargo, Patricia Fernanda Felipe Pinheiro, Marcelo Martinez, Carlos Roberto Padovani, Francisco Eduardo Martinez
Summary: The low and high consumption of ethanol in postpuberty impairs reproductive parameters and the development of ethanol-naive offspring, and these effects are dose-related.
REPRODUCTION AND FERTILITY
(2022)