Article
Biochemistry & Molecular Biology
Marta Carnovali, Giuseppe Banfi, Giovanni Porta, Massimo Mariotti
Summary: Foods can modulate inflammation and skeletal development, and the intestine plays a key role in bone health regulation. Soybean meal can induce intestinal inflammation in zebrafish, which affects different mechanisms of bone development in different zones of the skeleton.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Marta Carnovali, Roberto Valli, Giuseppe Banfi, Giovanni Porta, Massimo Mariotti
Summary: Inflammatory bowel disease has been found to be associated with low bone mineral density. Zebrafish is a suitable model for studying the effects of intestinal inflammation on bone metabolism. Research shows that soybean meal can induce intestinal inflammation in zebrafish, leading to changes in bone structure and activity of bone cells.
Article
Physiology
Aobo Pang, Cong Peng, Ruitao Xie, Zhuoduo Wang, Beiping Tan, Tingting Wang, Wei Zhang
Summary: This study investigated the effects of replacing fish meal with fermented soybean meal (FSBM) on the growth performance, intestinal morphology, immunity, and microbiota of the pearl gentian grouper. The results showed that the FSBM diets significantly reduced the growth of the fish and caused pathological manifestations of intestinal inflammation. Gene expression levels and the composition of the gut microbiota were also affected by the FSBM diets. The study highlights the negative role of dietary FSBM on intestinal flora and calls for more attention to be paid to this issue.
FRONTIERS IN PHYSIOLOGY
(2023)
Article
Gastroenterology & Hepatology
Zheng Zhou, Wenjing Yang, Tianming Yu, Yu Yu, Xiaojing Zhao, Yanbo Yu, Chuncai Gu, Anthony J. Bilotta, Suxia Yao, Qihong Zhao, George Golovko, Mingsong Li, Yingzi Cong
Summary: This study investigated the role of GPR120 in regulating neutrophil function against enteric bacteria. Gpr120 (-/-) mice showed altered gut microbiota composition and exhibited more severe intestinal inflammation upon infection or DSS insults. GPR120 agonist enhanced neutrophil production of reactive oxygen species and extracellular traps, leading to inhibition of bacterial growth.
Article
Immunology
Jianhua Zhao, Xin Yang, Zongsheng Qiu, Rongfei Zhang, Hong Xu, Ting Wang
Summary: This study showed that supplementation of 1%-2% AGn and 0.1%-0.2% TB in high-level soybean meal diet can alleviate enteritis in largemouth bass caused by high-level soybean meal. The recommended dose is 2% AGn and 0.2% TB.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Fisheries
Vikas Kumar, Femi J. Fawole, Nicholas Romano, Md Sakhawat Hossain, Shyam N. Labh, Ken Overturf, Brian C. Small
Summary: The study demonstrated that supplementation of BSFL effectively prevented SBM-induced intestinal enteritis and enhanced innate immunity, while the use of BSFLO showed some immunological benefits, especially when plant-based proteins were used at elevated levels.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Pawel Serek, Monika Oleksy-Wawrzyniak
Summary: The intestinal barrier, composed of cells, mucus, and microbiota, plays a crucial role in maintaining immune homeostasis. Disturbances in its tightness can lead to a range of issues, including systemic infections and autoimmune diseases. Zonulin, a protein found in epithelial cells, plays a key regulatory role.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Isabela M. Di Chiacchio, Elena Gomez-Abenza, Isadora M. Paiva, Danilo J. M. de Abreu, Juan Francisco Rodriguez-Vidal, Elisangela E. N. Carvalho, Stephan M. Carvalho, Luis David Solis-Murgas, Victoriano Mulero
Summary: This study analyzed the effects of bee pollen on growth, gut microbial composition, and skin melanoma development in zebrafish. The results showed that bee pollen influenced the microbiota composition and melanoma development, but unexpectedly promoted tumor growth.
SCIENTIFIC REPORTS
(2022)
Article
Fisheries
Wentao Wang, Jinjin Zhang, Rui Shao, Shufei Liang, Hui Zhang, Weiqi Xu, Mingzhu Li, Qinghui Ai, Kangsen Mai, Min Wan
Summary: This study aimed to investigate the effects of acetate on turbot fed a diet with a high proportion of soybean meal (SBM). The results showed that the addition of 1.0% acetate effectively promoted fish growth, improved mucosal barrier and digestive enzyme activities in the intestine, alleviated intestinal inflammation and oxidative stress caused by high SBM diet, and increased the anti-infectious ability of turbot. Therefore, the supplementation of acetate can effectively alleviate the adverse effects of high SBM diet on turbot growth and intestinal health, and improve fish resistance to bacterial infection.
Article
Genetics & Heredity
Luis Valenzuela, Sebastian Pacheco, Gonzalo Rincon, Leonardo Pavez, Natalia Lam, Adrian J. Hernandez, Patricio Dantagnan, Felipe Gonzalez, Felipe Jilberto, M. Cristina Ravanal, Cecilia Ramos, Hector Garcia, Cristian Araneda, Pilar E. Ulloa
Summary: The study found that the tolerance of golden pompano to soybean meal is mainly due to transcriptomic changes in lipid metabolism. Upregulation of genes related to lipid metabolism and downregulation of genes related to immunity may be the reasons for suppressing inflammatory responses and promoting growth. Genotypic variations in DEGs could potentially serve as biomarkers for early selection of fish tolerant to soybean meal or other plant-based diets.
Article
Biochemistry & Molecular Biology
Meng Sun, Qing Liu, Qiuxia Liang, Shuo Gao, Kaiyan Zhuang, Yun Zhang, Huazheng Zhang, Kechun Liu, Gaimei She, Qing Xia
Summary: Toosendanin (TSN) from Toosendan Fructus has promising anti-tumor capacity but is suspected to be hepatotoxic. This study found that TSN treatment led to liver injury in zebrafish, and identified inflammation, autophagy, and apoptosis as mechanisms involved in TSN-induced hepatotoxicity.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2021)
Article
Cell Biology
Natalia Nowik, Tomasz K. Prajsnar, Anna Przyborowska, Krzysztof Rakus, Waldemar Sienkiewicz, Herman P. Spaink, Piotr Podlasz
Summary: Galanin, a conserved peptide found across different species, was traditionally thought to be related to neurotransmission but recent studies have shown its involvement in immune responses. Knockout of galanin in zebrafish increased susceptibility to Mycobacterium marinum and Staphylococcus aureus infections, while administration of a galanin analogue improved the fish's ability to control the infections. Transcriptional data revealed fewer gene regulations in response to mycobacterial infections in gal-/- mutants compared to gal+/+ counterparts, indicating a significant impact of galanin deficiency on immune-related pathways. The results suggest that galanin not only serves as a neurotransmitter, but also has implications in immune responses to bacterial infections, highlighting the complexity of the neuroendocrine system and its connection to immunity.
Article
Multidisciplinary Sciences
Li Xiaobin, Xie Jinglong, Zhao Fang, Wang Chenchen, Yang Kailun
Summary: The study found that HXBM408 bacteria can increase the degradation capability of soybean isoflavones shortly after ingestion, increase the number of beneficial intestinal flora, and improve the structure of the flora.
Article
Chemistry, Analytical
Zhenzhen Jia, Yujie Zhang, Chuanyu Zhang, Xueyong Wei, Mingzhen Zhang
Summary: In this study, a sensitive and user-friendly biosensor based on pregnancy test strips was developed to analyze IBD-associated biomarkers and provide auxiliary diagnosis of IBD. The results showed a correlation between IAP and intestinal inflammation, indicating the potential of IAP as a biomarker for IBD.
ANALYTICAL CHEMISTRY
(2023)
Article
Developmental Biology
Samuel Patula, Michal Wojno, Lee J. Pinnell, Frank Oliaro, Chrissy Cabay, Giovanni S. Molinari, Karolina Kwasek
Summary: The study demonstrates that nutritional programming has a significant impact on the utilization of plant protein and the gut microbiome in zebrafish.
Article
Fisheries
Ruben Avendano-Herrera, Isabel Benavides, Jaime A. Espina, Daniela Soto-Comte, Matias Poblete-Morales, Juan A. Valdes, Carmen G. Feijoo, Ariel E. Reyes
JOURNAL OF FISH DISEASES
(2020)
Article
Microbiology
Marjorie Pizarro-Guajardo, Paulina Calderon-Romero, Alba Romero-Rodriguez, Daniel Paredes-Sabja
FRONTIERS IN MICROBIOLOGY
(2020)
Article
Immunology
Camila J. Solis, M. Kristina Hamilton, Mario Caruffo, Juan P. Garcia-Lopez, Paola Navarrete, Karen Guillemin, Carmen G. Feijoo
FRONTIERS IN IMMUNOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Ana Raquel Maia, Rodrigo Reyes-Ramirez, Marjorie Pizarro-Guajardo, Anella Saggese, Ezio Ricca, Loredana Baccigalupi, Daniel Paredes-Sabja
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Materials Science, Biomaterials
Andreas Tapia-Arellano, Eduardo Gallardo-Toledo, Camilo Ortiz, Jonathan Henriquez, Carmen G. Feijoo, Eyleen Araya, Rodrigo Sierpe, Marcelo J. Kogan
Summary: The study demonstrated successful functionalization of gold nanoprisms with PEG and Ang2 for enhanced blood-brain barrier penetration, showing no toxicity in vitro and in vivo. The nanomaterial showed improved BBB crossing in a zebrafish larvae model, making it a promising candidate for biomedical applications in CNS-related diseases.
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
(2021)
Article
Multidisciplinary Sciences
Pablo Castro-Cordova, Paola Mora-Uribe, Rodrigo Reyes-Ramirez, Glenda Cofre-Araneda, Josue Orozco-Aguilar, Christian Brito-Silva, Maria Jose Mendoza-Leon, Sarah A. Kuehne, Nigel P. Minton, Marjorie Pizarro-Guajardo, Daniel Paredes-Sabja
Summary: Spores produced by Clostridioides difficile during infection play a crucial role in the disease recurrence. Entry of the spores into the intestinal mucosa via host fibronectin and vitronectin pathways, and inhibition of spore entry can lead to reduced recurrence of infection in a mouse model.
NATURE COMMUNICATIONS
(2021)
Article
Microbiology
Orlando Vargas, Maria Soledad Gutierrez, Mario Caruffo, Benjamin Valderrama, Daniel A. Medina, Katherine Garcia, Angelica Reyes-Jara, Magaly Toro, Carmen G. Feijoo, Paola Navarrete
Summary: The study investigates the impact of two probiotic yeasts on the microbiome and neutrophil response in zebrafish larvae, showing that they can modulate the microbiota configuration, reduce inflammation, and enhance survival rate. Yeast pre-inoculation prevents changes in key taxa and metabolic pathways induced by pathogen infection, highlighting the important role of yeasts in shaping the microbiome and influencing the survival of infected larvae.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Cell Biology
Nayaret Chamorro, David A. Montero, Pablo Gallardo, Mauricio Farfan, Mauricio Contreras, Marjorie De la Fuente, Karen Dubois, Marcela A. Hermoso, Rodrigo Quera, Marjorie Pizarro-Guajardo, Daniel Paredes-Sabja, Daniel Ginard, Ramon Rossello-Mora, Roberto Vidal
Summary: The study found that patients with inflammatory bowel diseases (IBDs) have dysbiosis characterized by an increase in pro-inflammatory bacteria and a decrease in beneficial bacteria. The development of the disease is related to alterations in the gut microbiota, but the mechanisms are not fully understood. The research also identified a panel of bacterial biomarkers that can distinguish between a healthy gut microbiota and dysbiosis with high accuracy, which could be used for developing non-invasive diagnostic methods.
Editorial Material
Immunology
Nan Wu, Rune Waagbo, Min Wan, Carmen G. Feijoo, Wei-Dan Jiang
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Narges Aghaallaei, Rashi Agarwal, Joergen Benjaminsen, Katharina Lust, Baubak Bajoghli, Joachim Wittbrodt, Carmen G. Feijoo
Summary: The study reveals the existence of a specific region in the intestine of medaka fish where interaction between Ccl25-expressing cells and Ccr9a(+) T cells occurs. This interaction is observed during both homeostasis and inflammation, with a higher frequency and duration during inflammation. The findings suggest that the mucosal immune response in the medaka fish intestine is organized and orchestrated by this specific region.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yolanda Prado, Cesar Echeverria, Carmen G. G. Feijoo, Claudia A. A. Riedel, Claudio Cabello-Verrugio, Juan F. F. Santibanez, Felipe Simon
Summary: Sepsis syndrome is characterized by enhanced secretion of pro-inflammatory cytokines and reactive oxygen species, leading to vascular hyperpermeability, hypotension, multiple organ dysfunction syndrome (MODS), and increased mortality. Endotoxemia-derived sepsis plays an important role in sepsis syndrome, where endotoxin interacts with endothelial cells and induces detrimental effects. Nutrient consumption, including omega-3 fatty acids, ascorbic acid, and polyphenolic antioxidant flavonoids, modulates the progression of sepsis. This study aimed to investigate the impact of dietary supplementation with these nutrients on the modulation of fibrotic endothelial gene-expression patterns during sepsis and their effects on sepsis outcomes. The findings suggest that the consumption of omega-3 fatty acids and polyphenolic antioxidant flavonoids improves endotoxemia outcomes, highlighting the potential role of specific nutrient consumption in sepsis treatment.
Article
Biochemistry & Molecular Biology
Yolanda Prado, Pablo Tapia, Felipe Eltit, Cristian Reyes-Martinez, Carmen G. Feijoo, Felipe M. Llancalahuen, Claudia A. Riedel, Claudio Cabello-Verrugio, Jimmy Stehberg, Felipe Simon
Summary: Sepsis syndrome is a highly lethal uncontrolled response to infection, characterized by coagulopathy. Oxidized high-density lipoprotein (oxHDL) plays a role in promoting hemostatic dysfunction in septic-shock patients (SSP), leading to a prothrombotic phenotype. A study on 42 patients (26 SSP and 16 non-SSP) and 39 healthy volunteers found that increased plasma oxHDL level in SSP was associated with increased mortality and elevated risk of death, indicating its predictive value. The underlying mechanism involves endothelial protein expression regulation mediated by the LOX-1 receptor.
Correction
Multidisciplinary Sciences
Juan P. Garcia-Lopez, Alexandre Grimaldi, Zelin Chen, Claudio Meneses, Karina Bravo-Tello, Erica Bresciani, Alvaro Banderas, Shawn M. Burgess, Pedro P. Hernandez, Carmen G. Feijoo
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Juan P. Garcia-Lopez, Alexandre Grimaldi, Zelin Chen, Claudio Meneses, Karina Bravo-Tello, Erica Bresciani, Alvaro Banderas, Shawn M. Burgess, Pedro P. Hernandez, Carmen G. Feijoo
Summary: This study characterizes the developmental origins and functional differences of neutrophils derived from rostral blood island and caudal haematopoietic tissue lineages in zebrafish. The result shows that rostral neutrophils exhibit higher expression of csf3b and migration capacity compared to caudal neutrophils in steady state. Both populations have a core transcriptional profile upon injury, but caudal neutrophils respond heterogeneously. These findings reveal the functional differences of co-existing neutrophil populations with distinct developmental origins.
NATURE COMMUNICATIONS
(2023)
Article
Biology
Ivanka Jimenez-Dinamarca, Yolanda Prado, Pablo Tapia, Sebastian Gatica, Clemens Alt, Charles P. Lin, Cristian Reyes-Martinez, Carmen G. Feijoo, Cristobal Aravena, Alejandra Gonzalez-Canacer, Simon Correa, Diego Varela, Claudio Cabello-Verrugio, Felipe Simon
Summary: This study found that TRPM7 in endothelial cells regulates coagulation during sepsis, contributing to inflammation and increased mortality. Additionally, TRPM7 expression in circulating endothelial cells accurately predicts mortality in septic shock patients, making it a potential prognostic biomarker and target for treating sepsis-related coagulation disorders.
BIOLOGICAL RESEARCH
(2023)