Article
Chemistry, Analytical
Xin Hua, Xi Liu, Qian Zhu, Yu Liu, Sisi Zhou, Puzhen Huang, Quan Li, Songqin Liu
Summary: Recent studies have discovered a new extracellular secretion pathway for autophagosomes, which is closely related to cancer development. A three-dimensional microfluidic chip was developed to efficiently capture and detect secretory autophagosomes, showing promising potential for cancer diagnosis.
ANALYTICAL CHEMISTRY
(2022)
Article
Biotechnology & Applied Microbiology
Christoph Keysberg, Helga Schneider, Kerstin Otte
Summary: This study presents a purification setup using tangential flow filtration (TFF) and fast-performance liquid chromatography (FPLC)-based size exclusion chromatography (SEC) suitable for industrial production of therapeutic extracellular vesicles (EVs). Potential host cell lines were evaluated, with telomerase-immortalized Wharton's jelly mesenchymal stromal cells (WJ-MSC/TERT273) found to secrete high amounts of regenerative EVs, and Cevec's amniocyte producer cells (R) (CAP (R)) and human embryonic kidney (HEK293) suspension cells suitable for designer EVs with high yields. Chemical adjuvants were used to enhance EV secretion in HEK293 cells, with the combination of fenoterol, iodoacetamide, and dinitrophenol increasing EV yield threefold and cellular secretion rate fivefold.
BIOTECHNOLOGY AND BIOENGINEERING
(2023)
Review
Chemistry, Analytical
Ning Zhang, Haolin Chen, Chenjie Yang, Xufang Hu, Nianrong Sun, Chunhui Deng
Summary: This article reviews the recent progress of nanomaterials in the separation and analysis of extracellular vesicles, focusing on the applications of nanomaterials in the enrichment of extracellular vesicles and their contents. Some tips for exosome separation and analysis are briefly suggested.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2022)
Review
Chemistry, Inorganic & Nuclear
Jiayi Tan, Yu Wen, Ming Li
Summary: Exosomes, a class of extracellular vesicles secreted by cells, have been identified as promising diagnostic biomarkers for various diseases. Current research focuses on developing biosensors for exosomes with improved performance, aiming at practical applications in clinical and point-of-care diagnostics.
COORDINATION CHEMISTRY REVIEWS
(2021)
Article
Multidisciplinary Sciences
Shizhen Shen, Zhangjin Shen, Conghui Wang, Xiaodong Wu, Lingfang Wang, Lele Ye, Songfa Zhang, Xiaodong Cheng
Summary: In this study, a method for extracting extracellular vesicles (EVs) from epithelial ovarian cancer (EOC) tissues was optimized to investigate the effect of cryopreservation on the phenotypes, contents, and biological functions of the extracted EVs. The results showed that cryopreservation affected the yield and distribution of EVs, but not their morphology or particle mass ratio. The storage of tissues at -80°C increased both small and large EV yields, while the storage of lysates at -80°C decreased large EV yield and increased small EV yield. The cryopreserved EOC tissue retained a high percentage of proteins and miRNAs, and the extracted EVs could promote angiogenesis and invasive migration ability in human endothelial cells. This suggests that cryopreserved EOC tissue has clinical value for studying tissue-derived EVs, especially those related to EV proteins.
Article
Agriculture, Dairy & Animal Science
Seok Hee Lee, Saul Lira-Albarran, Islam M. Saadeldin
Summary: This study provides a comprehensive proteomic analysis of OC-EVs in dogs, revealing the involvement of these proteins in key cellular metabolic processes related to oocyte maturation and embryonic development. These findings offer valuable insights for understanding canine reproductive physiology and establishing an efficient in vitro embryo production system.
Article
Multidisciplinary Sciences
Joshua D. Spitzberg, Scott Ferguson, Katherine S. Yang, Hannah M. Peterson, Jonathan C. T. Carlson, Ralph Weissleder
Summary: This study developed a technique called MASEV for multiplexed analysis of EVs, which allows the interrogation of thousands of individual EVs using multi-channel fluorescence staining. The findings reveal the presence of multiple biomarkers in single vesicles and the potential loss of rare EV subtypes through affinity purification. These findings demonstrate the importance of MASEV in uncovering the heterogeneity and biology of EVs and improving diagnostic specificity.
NATURE COMMUNICATIONS
(2023)
Review
Chemistry, Multidisciplinary
Jie Wang, Peng Ma, Daniel H. Kim, Bi-Feng Liu, Utkan Demirci
Summary: Exosomes, nano-sized extracellular vesicles secreted by cells, have revolutionized various areas such as liquid biopsies, cancer biology, drug delivery, and immunotherapy. Microfluidic platforms show promise in overcoming the limitations of conventional techniques by offering high precision processing, microscale liquid handling, and integration with various functional units for efficient exosome separation and sensing.
Review
Chemistry, Multidisciplinary
Silvia Fischer, Elisabeth Deindl
Summary: Extracellular vesicles (EVs) are important for cell-to-cell communication by delivering various RNA, proteins, lipids, and nucleotides. Different types of EVs, including exosomes, cell surface-derived EVs, and apoptotic bodies, contain different RNA species and can be isolated, quantified, and characterized using specific methods.
APPLIED SCIENCES-BASEL
(2021)
Article
Chemistry, Multidisciplinary
Fatih Inci
Summary: Isolating nanoparticles from complex fluids is crucial in various fields, and a microfluidic device with integrated filters has been developed to achieve multiple uses for separating exosomes from cell culture media.
Review
Biochemistry & Molecular Biology
Hiu Yee Kwan, Minting Chen, Keyang Xu, Baisen Chen
Summary: This article critically reviews the impact of obesity on the exosomal cargo contents and the biogenesis of adipocyte-derived exosomes. It also discusses the differences in exosomal contents in different adipose depots, as well as the impact of obesity on exosomes derived from stromal vascular fraction components. The review also touches upon the development and potential application of exosome-based therapy for obesity and its comorbidities.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Nekane Romero-Garcia, Javier Huete-Acevedo, Cristina Mas-Bargues, Jorge Sanz-Ros, Mar Dromant, Consuelo Borras
Summary: The exponential growth in the elderly population and their associated socioeconomic burden have recently brought aging research into the spotlight. To integrate current knowledge and guide potential interventions, nine biochemical pathways are summarized under the term hallmarks of aging. These hallmarks are deeply inter-related and act together to drive the aging process. Altered intercellular communication is particularly relevant since it explains how damage at the cellular level translates into age-related loss of function at the organismal level. As the main effectors of intercellular communication, extracellular vesicles (EVs) might play a key role in the aggravation or mitigation of the hallmarks of aging. This review aims to summarize this role and provide context for the multiple emerging EV-based gerotherapeutic strategies that are currently under study.
Article
Chemistry, Multidisciplinary
Jelle Penders, Anika Nagelkerke, Eoghan M. Cunnane, Simon Pedersen, Isaac J. Pence, R. Charles Coombes, Molly M. Stevens
Summary: The study focuses on extracellular vesicles (EVs) and introduces a high-throughput label-free EV analysis method for cancer diagnosis and monitoring, eliminating interpreter bias. By developing a dedicated device and using dimensional reduction analysis (DRA), a convenient and comprehensive approach for comparing multiple EV spectra is demonstrated. The study shows the high sensitivity and specificity of the dedicated SPARTA system in differentiating cancer and noncancer EVs, and its consistent predictive ability across multiple EV isolations.
Review
Biochemistry & Molecular Biology
Yu-Chen Jia, Yi-Xuan Ding, Wen-Tong Mei, Yu-Ting Wang, Zhi Zheng, Yuan-Xu Qu, Kuo Liang, Jia Li, Feng Cao, Fei Li
Summary: Comprehensive reviews and large population-based cohort studies have significantly reduced the incidence and mortality of pancreatitis. The study of extracellular vesicles (EVs), especially exosomes, in pancreatitis has shown potential for identifying cell-to-cell communication and influencing the disease's development and progression. Plasma exosomes of acute pancreatitis can reach the alveolar cavity and activate alveolar macrophages to cause acute lung injury, suggesting their use as biomarkers and therapeutic agents for this disease.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2021)
Review
Endocrinology & Metabolism
Simon Scheck, Emily S. J. Paterson, Claire E. E. Henry
Summary: Endometriosis is a chronic inflammatory disease that has negative impacts on quality of life and fertility. Extracellular vesicles (EVs) play a role in the development of endometriosis and have potential for diagnosis and treatment. However, there are challenges in standardizing EV isolation techniques and reporting, as well as comparing studies due to variations in patient population and sample collection methods.
REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
(2022)