期刊
ENGINEERING IN LIFE SCIENCES
卷 15, 期 7, 页码 689-699出版社
WILEY
DOI: 10.1002/elsc.201400203
关键词
Bioreactor; Copper; Iron; Methanotrophs; Polyhydroxyalkaonate
资金
- Advanced Manufacturing Cooperative Research Centre (AMCRC) - Australian Government's Cooperative Research Centre Scheme [2.3.4]
- AMCRC PhD fellowship at James Cook University
Methane (CH4) is a potent greenhouse gas and mitigation is important to reduce global warming impacts. In this study, we aimed to convert CH4 to polyhydroxybutyrate (PHB; a biopolymer) by enrichment of methanotrophic consortia in bioreactors. Two different methanotrophic consortia were established form landfill top-cover (landfill biomass [LB]) and compost soils (compost biomass [CB]), through cultivation under CH4:CO2:air (30:10:60) in batch systems. The established cultures were then used as inoculi (0.5g LB or CBL-1) in continuous stirred tank reactors (CSTRs) aerated with CH4:CO2:air at 0.25Lmin(-1). Under stable CSTRs operating conditions, the effect of spiking with 1:1 copper:iron (final concentrations of 5M) was tested. Methane oxidation capacity (MOC), biomass dry weight (DWbiomass), PHB, and fatty acid methyl esters (FAMEs) contents were used as effect parameters. A maximum MOC of 481.9 +/- 8.9 and 279.6 +/- 11.3mg CH(4)g(-1) DW(biomass)h(-1) was recorded in LB-CSTR and CB-CSTR, respectively, but PHB production was similar for both systems, that is 37.7mgg(-1) DWbiomass. Treatment with copper and iron improved PHB production (22.5% of DWbiomass) in LB-CSTR, but a reduction of 13.6% was observed in CB-CSTR. The results indicated that CH4 to PHB conversion is feasible using LB-CSTRs and addition of copper and iron is beneficial.
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