Review
Microbiology
Karla Esquilin-Lebron, Sarah Dubrac, Frederic Barras, Jeffrey M. Boyd
Summary: Building iron-sulfur clusters and assembling Fe-S proteins are crucial for sustaining life, with genes coding for these proteins found in almost every sequenced genome. Defective assembly of Fe-S proteins can lead to cell death or metabolic defects.
Review
Chemistry, Inorganic & Nuclear
Ingie Elchennawi, Sandrine Ollagnier de Choudens
Summary: Tuberculosis (TB) is the leading cause of death by a single pathogen, resulting in 1.5 million deaths annually worldwide. Mycobacterium tuberculosis, the cause of TB, is exposed to various stresses that inhibit bacterial proliferation and survival. The SUF machinery, a complex protein assembly system involved in iron-sulfur cluster assembly, is an attractive target for the development of novel anti-TB drugs.
Review
Biochemistry & Molecular Biology
Corinne Cassier-Chauvat, Fanny Marceau, Sandrine Farci, Soufian Ouchane, Franck Chauvat
Summary: From bacteria to plants and humans, the glutathione system plays a crucial role in defense against various stresses and maintaining cellular homeostasis. Glutathione directly scavenges reactive oxygen species and serves as a cofactor for important enzymes involved in detoxification. This review focuses on the glutathione system in selected model organisms, with an emphasis on cyanobacteria due to their evolution of photosynthesis and the synthesis of important glutathione-derived metabolites. Cyanobacteria provide a unique genetic approach to analyze the specific roles and redundancy of the glutathione system components.
Review
Plant Sciences
Bing Yang, Chenyun Xu, Yuting Cheng, Ting Jia, Xueyun Hu
Summary: Iron-sulfur (Fe-S) clusters are ancient protein cofactors that exist ubiquitously in organisms and play important roles in various life processes. Plastids, semi-autonomous organelles believed to originate from cyanobacterial endosymbionts, have a Fe-S cluster biosynthesis and delivery pathway similar to cyanobacteria. Fe-S clusters are crucial for the normal functioning of downstream Fe-S proteins in plastids. Recent research has made significant progress in understanding this pathway and this review summarizes these findings as well as the remaining scientific challenges.
PLANT CELL REPORTS
(2023)
Article
Microbiology
Russell P. Swift, Krithika Rajaram, Rubayet Elahi, Hans B. Liu, Sean T. Prigge
Summary: Fd/FNR system plays a central role in the apicoplast of malaria parasites, providing reducing power to FeS proteins. This study found that Fd, FNR, and certain FeS proteins are essential for parasite survival, but not required for organelle maintenance. Additionally, the study demonstrated functional redundancy of SufA and NfuApi in delivering FeS clusters to Fd and other FeS proteins.
Article
Microbiology
Russell P. Swift, Krithika Rajaram, Rubayet Elahi, Hans B. Liu, Sean T. Prigge
Summary: The study revealed the importance of the Fdx/FNR system and FeS proteins in the survival of malaria parasites, as well as the redundant role of FeS transfer proteins in providing FeS clusters.
Article
Biochemistry & Molecular Biology
Jing Du, Zhaoyang Huang, Yanchun Li, Xueying Ren, Chaoting Zhou, Ruolan Liu, Ping Zhang, Guojie Lei, Jianxin Lyu, Jianghui Li, Guoqiang Tan
Summary: Copper is a necessary mineral nutrient but excess copper is cytotoxic. Wilson's disease is an autosomal recessive hereditary disease characterized by pathological copper accumulation in organs. The molecular mechanism of Wilson's disease is not fully understood and further research is needed to develop therapeutic strategies.
FREE RADICAL BIOLOGY AND MEDICINE
(2023)
Article
Biotechnology & Applied Microbiology
Xiaojun Ren, Feng Liang, Zhengfen He, Bingqian Fan, Zhirong Zhang, Xiudi Guo, Yukuan Du, Yilin Pang, Jianghui Li, Jianxin Lyu, Guoqiang Tan
Summary: This study demonstrates that under cold stress conditions, Escherichia coli's ferredoxin (Fdx) preferentially binds iron instead of [2Fe-2S] clusters, but can assemble clusters on its own as temperatures increase. The assembly of [2Fe-2S] clusters in Fdx follows a unique pathway distinct from the Isc or Suf systems.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Jing Zhang, Zechen Bai, Min Ouyang, Xiumei Xu, Haibo Xiong, Qiang Wang, Bernhard Grimm, Jean-David Rochaix, Lixin Zhang
Summary: Fe-S clusters are ancient and important for life processes. The biogenesis of Fe-S clusters involves iron acquisition, sulfur mobilization, and cluster formation. Two Arabidopsis DnaJ proteins, DJA6 and DJA5, play a crucial role in facilitating iron incorporation into Fe-S clusters during chloroplast Fe-S cluster biogenesis. Loss of these proteins leads to defects in chloroplast Fe-S protein accumulation and photosynthesis dysfunction. Evolutionary analysis shows that DJA6 and DJA5 are highly conserved in photosynthetic organisms and have a strong evolutionary relationship with SUFE1, SUFC, and SUFD.
Review
Chemistry, Inorganic & Nuclear
Jason C. Crack, Nick E. Le Brun
Summary: Iron-sulfur (FeS) clusters are protein cofactors essential for various biological functions such as electron transfer, catalysis, and gene regulation. Recent progress in using mass spectrometry has provided new insights into FeS cluster assembly and chemistry, particularly in transcriptional regulators coordinating cellular responses to changing conditions.
COORDINATION CHEMISTRY REVIEWS
(2021)
Review
Oncology
Jaewang Lee, Jong-Lyel Roh
Summary: Iron dysregulation is a characteristic of cancer, characterized by increased expression of genes involved in iron metabolism and iron-sulfur cluster (ISC) biogenesis. Dysregulated iron homeostasis leads to elevated intracellular labile iron, which can result in the formation of excessive cytotoxic radicals and susceptibility to regulated cell death, including ferroptosis. Various strategies, such as redox operations, iron chelation, and replacement of iron with redox-inert metals, can destabilize or restrict ISC formation and function, providing potential therapeutic approaches for cancer treatment. Targeting ISCs to induce ferroptosis shows promise in cancer therapy. This review provides a comprehensive overview of iron metabolism, ferroptosis, and the role of ISC modulation in cancer cells, as well as the potential of targeting ISCs for ferroptosis induction in cancer treatment. Further research and clinical trials are needed to validate these strategies for various cancers, with the goal of developing novel and effective cancer treatments.
Article
Biochemistry & Molecular Biology
Hassan Al-Tameemi, William N. Beavers, Javiera Norambuena, Eric P. Skaar, Jeffrey M. Boyd
Summary: S. aureus USA300 isolates utilize copBL and copAZ gene products to prevent Cu intoxication. A mutant strain lacking copAZ and copBL was sensitive to Cu and accumulated intracellular Cu. Mutations in the mntABC operon permitted growth in the presence of Cu, with transposon insertions in mntA being recessive. Defective MntABC resulted in decreased cellular Cu accumulation and protection to FeS enzymes from Cu poisoning.
MOLECULAR MICROBIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Naoki Shigi
Summary: Sulfur-containing biomolecules play crucial roles in cells, including enzyme cofactors, RNA composition, and regulation of gene translation efficiency. Modulating sulfur modification allows cells to adapt to different environments. Mutations in related genes can lead to diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Michael S. Petronek, Douglas R. Spitz, Bryan G. Allen
Summary: Cancer cells have a higher demand for iron due to their differential need for Fe-S biogenesis, which promotes cell growth and genomic stability. Researchers suggest that targeting Fe-S biogenesis through redox manipulation, Fe chelation, and Fe mimicry could be potential therapeutic strategies for cancer treatment.
Article
Cell Biology
Arthavan Selvanathan, Bindu Parayil Sankaran
Summary: Iron-sulfur clusters (ISCs) are highly conserved moieties found in crucial proteins in bacteria, plants and mammals, with defects in the ISC pathway being associated with various human disease states. These disorders often involve neurological phenotypes and share common biochemical features. Therapies are primarily supportive, but improved understanding of the pathophysiology may lead to disease-modifying therapies in the future.
Article
Microbiology
Guo-Wei Qiu, Hai-Bo Jiang, Hagar Lis, Zheng-Ke Li, Bin Deng, Jin-Long Shang, Chuan-Yu Sun, Nir Keren, Bao-Sheng Qiu
Summary: A novel class of substrate-selective iron porin, Slr1908, was found in the outer membrane of cyanobacteria, primarily involved in inorganic iron uptake. Widely distributed homologues of Slr1908 in freshwater and marine cyanobacteria play a role in tightly controlling iron flux into the cell, especially in environments with fluctuating iron concentrations.
ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Plant Sciences
Qi Yu, Zhong-Chun Zhang, Miao-Yu Wang, Alexander Scavo, Julian I. Schroeder, Bao-Sheng Qiu
Summary: The protein translation factor SaeIF1 plays an important regulatory role in the cadmium accumulation of Sedum alfredii by affecting Cd uptake, transportation, and detoxification, which contributes to the hyperaccumulation characteristics of the plant.
Article
Microbiology
Guo-Wei Qiu, Hagar Lis, Bao-Sheng Qiu, Nir Keren
Summary: Research indicates that cyanobacteria undergo a succession of physiological changes during iron deprivation and subsequent recovery. Cells show adaptive strategies to push metabolic limits under declining intracellular iron quotas, with only cells exhibiting high auto-fluorescence able to grow and reconstitute thylakoid membranes. Phenotypic heterogeneity within populations is crucial for survival and proliferation when facing iron fluctuations in natural environments.
ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Microbiology
Hai-Feng Xu, Guo-Zheng Dai, Yu-Jie Wang, Chao Cheng, Jin-Long Shang, Ren-Han Li, Ke Liu, Deqiang Duanmu, Bao-Sheng Qiu
Summary: Through genome analysis of a subaerial desert cyanobacterium, it was found that it contains various phytochromes and cyanobacteriochromes that can sense multiple wavelengths of light. Additionally, a CBCR capable of acclimating to weak light environments at dawn was identified.
ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Plant Sciences
Yaqiong Li, Min Chen
Summary: This study investigates the mechanism of photosynthesis in cyanobacteria by heterologously expressing phycobilisome-related genes and analyzing the construction of chromoproteins under different light conditions. The process of building phycobiliproteins under far-red light and the catalytic properties of phycobiliprotein lyases were revealed through experimental testing. The roles of specialized APC and contributions of PBP lyases were discussed, providing insights into the light-harvesting antenna complexes in cyanobacteria.
PHOTOSYNTHESIS RESEARCH
(2022)
Article
Plant Sciences
Miguel A. Hernandez-Prieto, Roger Hiller, Min Chen
Summary: Chlorophyll f, a new type of chlorophyll, allows oxygenic-photosynthetic organisms to thrive in environments with scarce white light but abundant far-red light. The study of its ligand properties and energy transfer characteristics reveals its role in extending the photosynthetic spectral range.
PHOTOSYNTHESIS RESEARCH
(2022)
Article
Plant Sciences
Li-Qin Shen, Zhong-Chun Zhang, Jin-Long Shang, Zheng-Ke Li, Min Chen, Renhui Li, Bao-Sheng Qiu
Summary: In this study, a strain of cyanobacteria capable of producing chlorophyll f under far-red light was taxonomically transferred to the genus Kovacikia and established as a new species called Kovacikia minuta sp. nov. Phylogenetic analysis revealed its relationship with other Kovacikia strains, and the draft genome provided insights into its genetic characteristics. This study contributes to our understanding of the diversity of chlorophyll f-producing cyanobacteria and lays a foundation for future research on the evolution of far-red light photoacclimation in cyanobacteria.
JOURNAL OF PHYCOLOGY
(2022)
Article
Microbiology
Fanyue Wang, Min Chen
Summary: Chromatic acclimation is a mechanism for optimizing light capture efficiency in cyanobacteria. Through genomic analysis, we identified different types of chromatic acclimation and their associated photoreceptors in cyanobacteria. We also found that different cyanobacteria have different capabilities for chromatic acclimation based on their genomic compositions and photoregulatory pathways.
Article
Plant Sciences
Alex Wu, Jason Brider, Florian A. Busch, Min Chen, Karine Chenu, Victoria C. Clarke, Brian Collins, Maria Ermakova, John R. Evans, Graham D. Farquhar, Britta Forster, Robert T. Furbank, Michael Groszmann, Miguel A. Hernandez-Prieto, Benedict M. Long, Greg Mclean, Andries Potgieter, G. Dean Price, Robert E. Sharwood, Michael Stower, Erik van Oosterom, Susanne von Caemmerer, Spencer M. Whitney, Graeme L. Hammer
Summary: Photosynthetic manipulation provides new opportunities for enhancing crop yield, but the understanding of its impact on crop growth and yield in different environments is limited. This study used simulations to predict the effects of altering photosynthesis on wheat and sorghum yield and uncovered the complex interactions between photosynthesis and crop dynamics.
PLANT CELL AND ENVIRONMENT
(2023)
Article
Multidisciplinary Sciences
Hai-Feng Xu, Guo-Zheng Dai, Yang Bai, Jin-Long Shang, Bin Zheng, De-Min Ye, Huazhong Shi, Aaron Kaplan, Bao-Sheng Qiu
Summary: This study identified a gene family of high light-inducible proteins (Hlips) that respond positively to dehydration in the Nostoc flagelliforme genome. Mutants lacking the Hlips gene cluster showed decreased desiccation tolerance due to impaired photosystem II repair, while heterologous expression of the Hlips gene cluster enhanced desiccation tolerance in Nostoc sp. PCC 7120. A transcription factor Hrf1 was also identified as a negative regulator for the adaptation of N. flagelliforme to the harsh desert environment. Phylogenetic analysis revealed the convergent evolution of desiccation tolerance through the coevolution of tandem Hlips duplication and Hrf1 in subaerial Nostoc species, providing insights into the mechanism of desiccation tolerance in photosynthetic organisms.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Plant Sciences
Li-Qin Shen, Zhong-Chun Zhang, Li Huang, Lu-Dan Zhang, Gongliang Yu, Min Chen, Renhui Li, Bao-Sheng Qiu
Summary: Two new species of cyanobacteria were identified, which can produce chlorophyll f, the fifth chlorophyll of oxygenic organisms, and can be induced to produce chlorophyll f under far-red light conditions. This study expands the diversity of chlorophyll f-producing cyanobacteria and provides valuable samples to study the essential binding sites of chlorophyll f within cyanobacterial photosystems.
JOURNAL OF PHYCOLOGY
(2023)
Article
Plant Sciences
Yi-Wen Yang, Ke Liu, Da Huang, Chen Yu, Si-Zhuo Chen, Min Chen, Bao-Sheng Qiu
Summary: Expanded orange carotenoid protein paralogs in subaerial Nostoc species have specialized functions as either singlet oxygen quenchers or phycobilisome fluorescence quenchers for survival in desiccation conditions. The different paralogs displayed different activities, with HCP2 being the most effective singlet oxygen quencher and OCPx1 exhibiting stronger phycobilisome fluorescence quenching compared to OCPx2. The crystal structures and mutant analysis revealed important roles for Trp111 and Met125 in OCPx2, which showed a more flexible regulation in energy quenching activities compared to OCPx1.