Article
Genetics & Heredity
Ninghong Li, Yingxue Dong, Min Lv, Li Qian, Xu Sun, Lin Liu, Yongping Cai, Honghong Fan
Summary: Dendrobium officinale flowers from different cultivars exhibit distinct smells and produce various volatile terpenoids, with transcriptome analysis identifying genes and transcription factors related to terpenoid biosynthesis, providing new insights into the biosynthetic mechanism of D. officinale flowers.
FRONTIERS IN GENETICS
(2021)
Article
Agricultural Engineering
Min Lv, Xu Sun, Decong Li, Guo Wei, Lin Liu, Feng Chen, Yongping Cai, Honghong Fan
Summary: This study provides novel insights into the catalytic and regulatory mechanisms underlying the biosynthesis of volatile terpenoids in Dendrobium officinale. Transcriptome analysis and terpene metabolic profiling identified a sesquiterpene synthase gene and several MYB genes involved in the biosynthesis and regulation of terpenoids. The study sheds light on the functional relationships between these genes and their roles in terpenoid biosynthesis.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Biochemistry & Molecular Biology
Danqi Zeng, Jaime A. Teixeira da Silva, Mingze Zhang, Zhenming Yu, Can Si, Conghui Zhao, Guangyi Dai, Chunmei He, Juan Duan
Summary: The study revealed that DoAP2 genes in D. officinale may play important roles in plant regeneration and flower development, with some DoAP2 genes down-regulated during flower development. Analysis using a dual-luciferase reporter gene system suggested that certain DoAP2 proteins may act as transcriptional repressors in the nucleus of plant cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Genetics & Heredity
Qianyu Yang, Weibo Xiang, Zhihui Li, Yuxin Nian, Xiaoyun Fu, Guangzhu Zhou, Linbao Li, Jun Zhang, Guiyun Huang, Xiao Han, Lu Xu, Xiao Bai, Lei Liu, Di Wu
Summary: In this study, the HD-ZIP gene family was identified and analyzed in Dendrobium officinale. The expression patterns of these genes in different tissues and under cold stress were investigated. Three key candidates, Dohdz5, Dohdz9, and Dohdz12, were identified as important genes involved in the response to cold stress.
FRONTIERS IN GENETICS
(2022)
Article
Plant Sciences
Maite Colinas, Jacob Pollier, Dries Vaneechoutte, Deniz G. Malat, Fabian Schweizer, Liesbeth De Milde, Rebecca De Clercq, Joana G. Guedes, Teresa Martinez-Cortes, Francisco J. Molina-Hidalgo, Mariana Sottomayor, Klaas Vandepoele, Alain Goossens
Summary: Catharanthus roseus produces a variety of MIA metabolites in a heavily branched pathway, with gene expression being highly specific to cell type, organ, and stress. Further study of ORCA TFs reveals subfunctionalization in terms of regulating target genes and working synergistically with MYC2. Specific amino acid residues within ORCA DNA-binding domains play a role in regulating MIA pathway branches differently.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Zhenming Yu, Guihua Zhang, Jaime A. Teixeira da Silva, Conghui Zhao, Jun Duan
Summary: In this study, a novel transcription factor DobHLH4 was identified in Dendrobium officinale and shown to positively regulate linalool biosynthesis by interacting with linalool synthase DoTPS10 and jasmonic acid pathway component DoJAZ1. Overexpression of DobHLH4 in D. officinale petals significantly increased linalool production by upregulating the expression of key biosynthetic genes, particularly DoTPS10. This suggests that DobHLH4 may serve as a promising target for metabolic engineering to enhance linalool production.
Article
Pharmacology & Pharmacy
Ming Lv, Qingqing Liang, Xiaofang He, Xiaocui Du, Yuhan Liu, Yan Liu, Chongye Fang
Summary: This study investigated the hypoglycemic effect and mechanism of D. officinale leaves. The results showed that D. officinale leaves significantly lowered blood glucose levels in mice on a high-fat diet, potentially by activating the insulin signaling pathway. Further examination revealed that the >3 kDa ethanol-soluble fraction of D. officinale leaves played a major role in lowering blood glucose.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Yasuyuki Yamada, Fumihiko Sato
Summary: Plants produce a diverse range of low-molecular-weight secondary compounds, with nitrogen-containing alkaloids being the most biologically active and commonly used in pharmaceuticals. Recent advancements in molecular biology technologies have facilitated a more detailed understanding of alkaloid biosynthesis pathways and the role of transcription factors in regulating these processes, leading to potential applications in alkaloid engineering.
Article
Multidisciplinary Sciences
Chun Yao Li, Susan Gibson
Summary: Decreasing the expression levels of negative transcriptional regulators ZCT1, ZCT2, and ZCT3 can effectively increase the expression of TIA biosynthetic genes in Catharanthus roseus. However, the impact of reducing ZCT levels on TIA biosynthetic genes varies, and may indirectly affect the TIA pathway by suppressing GBF1 expression.
Article
Agricultural Engineering
Sucheta Singh, Shiv S. Pandey, Rashmi Tiwari, Alok Pandey, Karuna Shanker, Alok Kalra
Summary: This study successfully increased the content and yield of medicinally important alkaloids in Catharanthus roseus plants by developing different microbial consortia, with varying effects observed in different seasons and cultivars.
INDUSTRIAL CROPS AND PRODUCTS
(2021)
Article
Plant Sciences
Elham Khataee, Farah Karimi, Khadijeh Razavi
Summary: The study compared the effects of different carbon sources on physiological aspects, especially medicinal alkaloid biosynthesis and related gene expression in Catharantus roseus. Both treatments showed incremental effects on alkaloid production and upregulated important genes in the biosynthetic pathway. Defensive responses were also enhanced, with sorbitol having a stronger effect.
FUNCTIONAL PLANT BIOLOGY
(2021)
Article
Plant Sciences
Chunyan Jiao, Mengke Wei, Honghong Fan, Cheng Song, Zhanjun Wang, Yongping Cai, Qing Jin
Summary: The study on Dendrobium officinale revealed that the total alkaloid content significantly increased after 4 hours of treatment with TIA precursors and MeJA. Transcriptome sequencing indicated enrichment of genes related to alkaloid biosynthetic pathways, as well as identification of genes encoding key enzymes and transcription factors regulating alkaloid biosynthesis in D. officinale. The findings provide insights into the regulatory mechanisms involved in the accumulation of alkaloids in D. officinale induced by TIA precursors and MeJA.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Chemistry, Applied
Shengchang Tao, Yuchen Song, Shaobo Ding, Ruirong He, Qiankun Shi, Fang Hu
Summary: Photodynamic therapy (PDT) eradicates tumors by generating toxic reactive oxygen species (ROS) using a photosensitizer (PS) and appropriate light. We enhanced the immune response to tumors after PDT by using a biocompatible herb polysaccharide as the carrier of the PS. The use of Dendrobium officinale polysaccharide (DOP) and positively charged nanoparticles significantly improved the immune response after PDT.
CARBOHYDRATE POLYMERS
(2023)
Article
Horticulture
Yang Liu, Shan-De Duan, Yin Jia, Li-Hong Hao, Di-Ying Xiang, Duan-Fen Chen, Shan-Ce Niu
Summary: Wild D. officinale is endangered, while cultivated D. officinale has inferior quality and low medicinal components. In this study, tetraploids of D. officinale were successfully generated using colchicine treatment. Morphological observations showed that tetraploids had smaller size, different leaf shape, and larger stem diameter. Cytological observations revealed larger stomata and fewer stomata per unit area in polyploid plants. Chromosomal observations showed that treatment with 0.2% colchicine for 48 hours resulted in the highest tetraploid induction rate.
Article
Plant Sciences
Zhengfei Luo, Lian Liu, Qiong Nie, Mingjin Huang, Chunlii Luo, Yedong Sun, Yongyan Ma, Jianxin Yu, Fuqiang Du
Summary: This study analyzed the secondary metabolites and antioxidant activity of D. officinale from three regions using a metabolomics approach. A total of 411 metabolites were identified, including 136 differential metabolites. These differential metabolites were mainly enriched in secondary metabolic pathways, particularly flavonoids, phenolic acids, and amines. Furthermore, correlation analysis revealed that 48 differential metabolites were significantly positively correlated with antioxidant capacity, with flavonoids being the main influencing factors. These findings provide valuable guidance for variety screening, quality control, and product development of D. officinale.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Polymer Science
Chen Song, Ying You, Chengrong Wen, Yinghuan Fu, Jingfeng Yang, Jun Zhao, Shuang Song
Summary: Low-molecular-weight carrageenan has advantages in solubility, absorption efficiency, and bioavailability compared to original carrageenan. A photocatalytic degradation method with only TiO2 has been developed to prepare low-molecular-weight carrageenan with high sulfation degree and low viscosity. The study also proposed the degradation rules and provided an effective green method for the degradation of carrageenan.
Article
Chemistry, Multidisciplinary
Xingying Zhang, Chen Song, Huijia Nong, Kaige Xu, Xiaozhuo Wu, Wen Zhong, Malcolm Xing, Leyu Wang
Summary: An asymmetrical DIW hydrophobic/hydrophilic membrane was developed using heat-processed graphene oxide (GO) ink. Additionally, a Masked spin coating method was utilized to create a microscale GO/hydrophilic reduced GO (rGO) physiological sensor and a macroscale moisture-driven GO/rGO actuator. Depositing mussel-inspired polydopamine (PDA) coating on the one side of the DIW rGO resulted in a flexible and moisture-responsive PDA-rGO/hydrophilic rGO microlattice (ECP) that could attach to the curved surface of the epicardium without gaps. This conformable ECP demonstrated promising therapeutic effects on rats' infarcted hearts by reconstructing the conductive microenvironment and enhancing neovascularization.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Engineering, Chemical
Jiarui Wang, Xiaocheng Peng, Chen Song, Jian Wen, Simin Wang
Summary: Improving the separation efficiency of fine particles is crucial in the face of worsening environmental pollution. This study investigates the impact of four key parameters on gas cyclone performance: cyclone body height, particle concentration, initial supersaturation degree, and inlet temperature. Through the use of a numerical model and optimization techniques, the research identifies the key factors affecting separation efficiency and provides important guidance for optimization and practical application.
CANADIAN JOURNAL OF CHEMICAL ENGINEERING
(2023)
Article
Engineering, Chemical
Fabian Hartung, Billy Joe Franks, Tobias Michels, Dennis Wagner, Philipp Liznerski, Steffen Reithermann, Sophie Fellenz, Fabian Jirasek, Maja Rudolph, Daniel Neider, Heike Leitte, Chen Song, Benjamin Kloepper, Stephan Mandt, Michael Bortz, Jakob Burger, Hans Hasse, Marius Kloft
Summary: This paper provides a comprehensive evaluation and analysis of modern unsupervised anomaly detection methods based on deep learning for chemical process data. The Tennessee Eastman process dataset is focused on as a standard benchmark for evaluating anomaly detection methods for almost three decades. The study will aid in selecting appropriate anomaly detection methods for industrial applications. Reconstruction-based methods are found to be the preferred choice, followed by generative and forecasting-based methods.
CHEMIE INGENIEUR TECHNIK
(2023)
Review
Engineering, Chemical
Marco Gaertler, Martin Hollender, Benjamin Kloepper, Sylvia Maczey, Ruomu Tan, Chen Song, Franz David Baehner, Stefan Kraemer, Gregor Just, Valentin Khaydarov, Leon Urbas, Rebecca Gedda
Summary: This paper provides an overview of several machine learning approaches that can be used in an industrial setting to automatically label batch phases based on sensor data.
CHEMIE INGENIEUR TECHNIK
(2023)
Article
Neurosciences
Chen Song, Yishu Yin, Yue Qin, Tianzhu Li, Deyong Zeng, Ting Ju, Fangyuan Duan, Yingchun Zhang, Weihong Lu
Summary: This study found that Acanthopanax senticosus extract has a protective effect on radiation-induced learning and memory impairment. In the experiment with mice, the extract improved learning and memory ability, changed neurotransmitter levels in the hippocampus and colon, and caused changes in the gut microbiota. The extract also increased expression of tight junction proteins in the colon, inhibited inflammation, and influenced protein expression in the hippocampus.
CNS NEUROSCIENCE & THERAPEUTICS
(2023)
Article
Immunology
Yuan Liu, Lanyu Wang, Wenyi Du, Yukang Huang, Yi Guo, Chen Song, Zhiqiang Tian, Sen Niu, Jiaheng Xie, Jinhui Liu, Chao Cheng, Wei Shen
Summary: The objective of this study was to develop a machine learning algorithm to identify high-risk factors for mortality in patients diagnosed with gastric cancer during and prior to treatment. The results showed that the XGBoost algorithm demonstrated superior predictive capacity for risk factors affecting mortality in gastric cancer patients one year, three years, and five years posttreatment. Key prognostic factors included age, tumor invasion, lymph node metastasis, peripheral nerve invasion, multiple tumors, tumor size, carcinoembryonic antigen (CEA) level, carbohydrate antigen 125 (CA125) level, carbohydrate antigen 72-4 (CA72-4) level, and H. pylori infection.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Plant Sciences
Xiaomei He, Wenwu Zhang, Irfan Ali Sabir, Chunyan Jiao, Guohui Li, Yan Wang, Fucheng Zhu, Jun Dai, Longyun Liu, Cunwu Chen, Yingyu Zhang, Cheng Song
Summary: In this study, differentially expressed genes involved in the JA signaling pathway were identified and the critical roles of the bHLH family, particularly the MYC2 subfamily, were revealed. Evolutionary analysis showed that whole genome duplication and segmental duplication events drove the expansion and functional divergence of bHLH genes. Multiple sequence alignments demonstrated the presence of conserved domains in all bHLH proteins. Expression profiling and qRT-PCR results suggested that bHLH subgroups IIIe and IIId may have an antagonistic role in the JA-mediated expression of stress-related genes.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Multidisciplinary Sciences
Qianyue Li, Xin Cheng, Chen Song, Taigang Liu
Summary: In this study, a deep learning model called m6A-BERT-Stacking was proposed to accurately detect m6A sites in various tissues of three species, which is critical for revealing their biological functions and influence on diseases.
Article
Ecology
Boyuan Zhu, Jianghui Meng, Renfang Pan, Haiyan Hu, Chen Song, Zhengping Zhu, Jineng Jin
Summary: By analyzing and modeling the Longmaxi Formation in Luzhou, southern Sichuan Basin, this study finds that the development of mesopores in organic matter is the key factor for the formation of high-quality reservoirs. An improved model is proposed to accurately predict organic matter porosity. This research is of great significance for the classification and understanding of shale gas reservoirs.
FRONTIERS IN ECOLOGY AND EVOLUTION
(2023)
Article
Biochemistry & Molecular Biology
Yutong Li, Juntao Bie, Long Zhao, Chen Song, Tianzhuo Zhang, Meiting Li, Changjiang Yang, Jianyuan Luo
Summary: SLC25A51 is upregulated in multiple cancers and promotes cancer cells proliferation. Loss of SLC25A51 elevates mitochondrial proteins acetylation levels due to SIRT3 dysfunctions, leading to impaired P5CS enzymatic activity and reduced proline contents. Fludarabine phosphate, an FDA-approved drug, inhibits SLC25A51 functions and decreases mitochondrial NAD(+) and induces proteins hyperacetylation, synergizing with aspirin to enhance anti-tumor efficacy.
CELL DEATH AND DIFFERENTIATION
(2023)
Article
Horticulture
Muhammad Aamir Manzoor, Guohui Li, Wang Xinya, Mengna Wang, Yu Zhao, Irfan Ali Sabir, Iftikhar Hussain Shah, Han Wang, Muhammad Abdullah, Gyung-Tae Kim, Cheng Song, Yongping Cai
Summary: This study systematically characterized and named 15 PbAL genes in Pyrus bretschenedri, and found that they could be classified into six subfamilies. Expression patterns of PbAL genes varied in different organs and fruit development stages, and stress-related cis-acting elements were found in their promoter regions. Furthermore, PbAL genes showed differential expression under IAA, GA, ME, and ABA treatments.
SCIENTIA HORTICULTURAE
(2023)
Article
Plant Sciences
Guohui Li, Muhammad Aamir Manzoor, Guoyu Wang, Cunwu Chen, Cheng Song
Summary: This study investigated the KNOX gene family in the Dendrobium huoshanense genome using bioinformatics methods. Phylogenetic relationship and promoter analysis revealed the potential roles of these genes in plant development and stress responses. qRT-PCR experiments confirmed the differential expression of DhKNOX genes under different treatments.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Horticulture
Cheng Song, Muhammad Aamir Manzoor, Di Mao, Xiang Ren, Wenwu Zhang, Yingyu Zhang
Summary: This study found that the application of melatonin significantly enhanced various aspects of tomato plant growth and reduced the damage caused by cadmium stress. By increasing the activity of antioxidant enzymes and decreasing the levels of oxidative stress markers, melatonin improved the resistance of tomato plants to cadmium stress.
SCIENTIA HORTICULTURAE
(2024)
Article
Plant Sciences
Peipei Wei, Yaobiqi Li, Cheng Song, Muhammad Aamir Manzoor, Jun Dai, Qihui Yin, Yanmei Zhang, Bangxing Han
Summary: This study investigated the correlation between the expression of key enzyme genes and the accumulation of coumarin content in different growth and development phases of Peucedanum praeruptorum Dunn. The results revealed the regulatory role of key enzymes in controlling coumarin production and accumulation in the plant.
ACTA PHYSIOLOGIAE PLANTARUM
(2023)
