Review
Biotechnology & Applied Microbiology
Serik Eliby, Sara Bekkuzhina, Olena Kishchenko, Gulnur Iskakova, Gulnar Kylyshbayeva, Satyvaldy Jatayev, Kathleen Soole, Peter Langridge, Nikolai Borisjuk, Yuri Shavrukov
Summary: Doubled haploid production is a valuable biotechnology that accelerates the breeding of new wheat varieties and plays important roles in studying genetic control, marker-assisted selection, genomics, and genetic engineering in wheat. This paper reviews recent advances in androgenesis and gynogenesis techniques, with a focus on the in vitro culture phase, and the emerging innovative approaches in researching and producing wheat doubled haploids. The use of CRISPR-based genome editing for inducing doubled haploids in wheat is also discussed.
BIOTECHNOLOGY ADVANCES
(2022)
Review
Plant Sciences
Isidre Hooghvorst, Salvador Nogues
Summary: The doubled haploid technique aims to generate pure inbred lines through haploid plant generation and chromosome doubling. Efforts have been focused on haploid production methods such as androgenesis, gynogenesis, and parthenogenesis. Recent interest has been in optimizing chromosome doubling methods to increase rates and efficiency of doubled haploid plant production.
PLANT CELL REPORTS
(2021)
Review
Agronomy
Meisam Zargar, Tatiana Zavarykina, Sergey Voronov, Irina Pronina, Maryam Bayat
Summary: Doubled haploid (DH) plants, with a doubled set of chromosomes, speed up the selection process by fixing genetic traits in a homozygous state in each locus within one generation. Recent research focuses on the production of doubled haploids in vivo and their fundamental bases, exploring mechanisms such as parthenogenesis induction, genome elimination during interspecific hybridization, and centromeric histone 3 (CENH3) modification.
Article
Plant Sciences
Csaba Lantos, Mihaly Jancso, Arpad Szekely, Eva Nagy, Timea Szaloki, Janos Pauk
Summary: Doubled haploid (DH) plant production, such as anther culture (AC), is a useful tool in modern rice breeding. This study compared the effects of different combinations of plant growth regulators on callus induction in rice, and identified the best combination for in vitro green plant production.
Article
Plant Sciences
Csaba Lantos, Mihaly Jancso, Arpad Szekely, Timea Szaloki, Shoba Venkatanagappa, Janos Pauk
Summary: An efficient protocol for in vitro anther culture in rice breeding, especially for indica genotypes, was evaluated. Three different induction media (N6NDK, N(6)NDZ, Ali-1) and four plant regeneration media (mMSNBK1, MSNBK3, MSNBKZ1, MSNBKZ2) were tested to increase the efficiency of in vitro androgenesis. The production of calli and green plantlets varied among different genotypes and media combinations. Flow cytometric analyses showed that 51.89% of the acclimatized indica rice plantlets had undergone spontaneous chromosome doubling. The utilization of DH plants has been integrated into the Hungarian rice breeding program, and tetraploid lines offer potential for future research and breeding directions.
Article
Plant Sciences
Samira Tajedini, Baratali Fakheri, Mohsen Niazian, Nafiseh Mahdinezhad, Abdelbagi Mukhtar Ali Ghanim, Ali Kazemi Pour, Ivan Ingelbrecht, Mehran E. Shariatpanahi
Summary: Two different androgenesis pathways, shed-microspore culture (SMC) and anther culture (AC), were developed for haploid induction in mutagenized M-1 rice cultivars. The effects of various factors on induction and regeneration phases were evaluated. The SMC method showed better results in terms of the number of regenerated embryos. Chromosome counting and flow cytometry analysis confirmed the presence of haploid plants, providing a valuable foundation for rice breeding research.
JOURNAL OF PLANT GROWTH REGULATION
(2023)
Article
Biotechnology & Applied Microbiology
Renata Orlowska
Summary: The study investigates the relationship between copper ions and DNA methylation in triticale regeneration, suggesting that copper ions affect regeneration efficiency through cytosine methylation and may induce mutations.
JOURNAL OF APPLIED GENETICS
(2022)
Article
Biotechnology & Applied Microbiology
Yuki Sasaki, Masahiro Komeno, Akihiro Ishiwata, Ayako Horigome, Toshitaka Odamaki, Jin-Zhong Xiao, Katsunori Tanaka, Yukishige Ito, Kanefumi Kitahara, Hisashi Ashida, Kiyotaka Fujita
Summary: Gum arabic arabinogalactan protein (AGP) can be effectively degraded by the cooperative action of GAfase, exo-beta 1,3-galactanase (Bl1,3Gal), and BlArafE, leading to the production of alpha-L-Rhap-(1 -> 4)-beta-D-GlcpA-(1-6)-beta-D-Galp-(1 -> 6)-D-Gal tetrasaccharide. This oligosaccharide can be utilized by commensal bacteria, potentially facilitating cross-feeding interactions between bifidobacteria and other gut microbial groups.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Agronomy
Stanislava Grozeva, Velichka Todorova, Amol N. Nankar
Summary: The Balkan pepper breeding program aims to use in vitro embryogenesis to develop doubled haploid pepper lines with valuable traits. The varieties Stryama and breeding line 560/06 showed the highest androgenic potential, with a successful adaptation rate of 79.03% for regenerated plants. Flow cytometry analysis confirmed all diploid plants to be androgenic in origin.
Article
Biochemistry & Molecular Biology
Kamil Zielinski, Ewa Dubas, Zuzana Gersi, Monika Krzewska, Agnieszka Janas, Anna Nowicka, Ildiko Matusikova, Iwona Zur, Shohei Sakuda, Jana Moravcikova
Summary: This study reports on the biochemical, cytochemical, and molecular studies on PR proteins and AGP during the early stages of androgenesis induction in rye breeding lines. The initiation of androgenesis was associated with high activity of beta-1,3-glucanases and chitinases, with some isoforms of beta-1,3-glucanases being anther-specific. Additionally, stress pre-treatments were found to promote AGP accumulation in the androgenesis-responsive rye line.
Review
Plant Sciences
Brett Hale, Alison M. R. Ferrie, Sreekala Chellamma, J. Pon Samuel, Gregory C. Phillips
Summary: Androgenesis is widely used in plant breeding programs to redirect cell fate and study cell cycle regulation, meiotic recombination, and embryogenesis. The focus has shifted from protocol development to understanding how protocols work in order to extend their application to other genotypes and species. Transcriptomic and epigenetic analyses have revealed cellular and molecular responses associated with androgenesis, and gene transfer and genome editing technologies show promise for enhancing the genetic composition of microspores used in breeding.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Paul Chege, Erzsebet Kiss, Csaba Lantos, Andrea Palagyi, Janos Pauk
Summary: The study aimed at producing doubled haploids of sorghum successfully from five genotypes, leading to the development of an improved protocol. Temperature and light conditions were found to play a crucial role in the successful process of regeneration and rooting, with sorghum's high tillering ability and abiotic stress tolerance observed to contribute to the attainment of haploid plantlets.
PHYTON-INTERNATIONAL JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Article
Cell Biology
Harsha Samtani, Aishwarye Sharma, Paramjit Khurana
Summary: In this study, DH wheat plants overexpressing the HVA1 gene were found to be tolerant to both drought and heat stress. Transcriptome analysis revealed the upregulation of transcription factors DREB and HsfA6 under drought and heat stress, respectively, and their role in providing heat tolerance through the ABA-mediated pathway. This study highlights the potential role of HVA1 in the heat stress signaling pathway.
Review
Biology
Ricardo Mir, Antonio Calabuig-Serna, Jose M. Segui-Simarro
Summary: This review compiles the latest advances in doubled haploid plant production, the application of doubled haploid lines in breeding programs, and future perspectives for alternative technologies for doubled haploid generation in eggplant. Eggplant is globally cultivated for its edible fruit, and breeding programs aim to produce F1 hybrids by crossing highly homozygous pure lines. Doubled haploid individuals can be induced from haploid cells in a single generation, offering a more efficient alternative to traditional self crossing methods.
Article
Plant Sciences
Carolina Camacho-Fernandez, Jose M. Segui-Simarro, Ricardo Mir, Kim Boutilier, Patricia Corral-Martinez
Summary: Different types of embryogenic structures exhibit specific cell wall characteristics that determine their developmental fate. Highly embryogenic structures have thin walls rich in specific components, while less embryogenic structures have thick walls and lower values of cell wall components.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Agronomy
M. Tyrka, S. Oleszczuk, J. Rabiza-Swider, H. Wos, M. Wedzony, J. Zimny, A. Ponitka, A. Slusarkiewicz-Jarzina, R. J. Metzger, P. S. Baenziger, A. J. Lukaszewski
MOLECULAR BREEDING
(2018)
Review
Plant Sciences
Joanna Chojak-Kozniewska, Elzbieta Kuzniak, Janusz Zimny
FRONTIERS IN PLANT SCIENCE
(2018)
Article
Plant Sciences
Sylwia Oleszczuk, Natalia Grzechnik, Annaliese S. Mason, Janusz Zimny
PLANT CELL REPORTS
(2019)
Article
Biotechnology & Applied Microbiology
Renata Orlowska, Katarzyna A. Pachota, Joanna Machczynska, Agnieszka Niedziela, Katarzyna Makowska, Janusz Zimny, Piotr T. Bednarek
ELECTRONIC JOURNAL OF BIOTECHNOLOGY
(2020)
Article
Plant Sciences
Renata Orlowska, Janusz Zimny, Piotr T. Bednarek
Summary: In this study, the role of copper ions as a mediator of DNA sequence variation in the CHG context was highlighted while silver ions did not show any mediating effect. Modifications in in vitro culture conditions explained DNA sequence variation, demethylation, and de novo methylation in the CHG context. Incorporating copper ions in the induction medium should be approached cautiously.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Agronomy
Janusz Zimny, Slawomir Sowa, Piotr Otreba, Janusz Kozdoj, Aleksandra Zimny, Joanna Kaczmarek, Sylwia Oleszczuk, Andrzej Czaplicki, Malgorzata Jedryczka
Summary: The study on transgenic winter triticale in field experiments in central Poland reveals that factors such as temperature increase, wind strength, and direction have a positive impact on pollen grain dispersal. Lower amounts of transgenic pollen grains were observed at a distance of 85 meters, with potential migration distances up to 100 meters in extreme atmospheric conditions.
Article
Biochemistry & Molecular Biology
Krzysztof Michalski, Christian Hertig, Dariusz R. Mankowski, Jochen Kumlehn, Janusz Zimny, Anna M. Linkiewicz
Summary: Cas endonuclease-mediated genome editing offers a long-awaited molecular biological approach for modifying predefined genomic target sequences in living organisms. The implementation of this technology can be challenging for species like triticale, but pre-validated cas9/gRNA constructs provide a strong foundation for the targeted induction of heritable modifications in triticale genes. The study also demonstrates that in-del induction frequency in triticale can be increased by TREX2 nuclease activity, regardless of gRNA performance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Agronomy
Katarzyna Makowska, Sylwia Oleszczuk, Janusz Zimny
CZECH JOURNAL OF GENETICS AND PLANT BREEDING
(2017)
